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http://dx.doi.org/10.5657/kfas.2005.38.6.379

Analysis of Oxolinic Acid in Fish Products Using HPLC  

LEE Hee Jung (National Fisheries Research and Development Institute)
LEE Tae Seek (National Fisheries Research and Development Institute)
SON Kwang Tae (National Fisheries Research and Development Institute)
KIM Poong Ho (National Fisheries Research and Development Institute)
JO Mi Ra (National Fisheries Research and Development Institute)
PARK Mi Jung (National Fishes Products Quality Inspection Service)
Yi Young Ho (The National Assembly)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.38, no.6, 2005 , pp. 379-384 More about this Journal
Abstract
A high-performance liquid chromatography assay method for oxolinic acid in fish products was developed, evaluated and validated through the monitoring of oxolinic acid based on farming and distribution. The recovery rate of the developed method was $102.3-106.7\%$ as compared to conventional methods. The stock solution was stable for 3 weeks under refrigerated condition at $4^{\circ}C$ The performance limit was evaluated as 0.01ppm of oxolinic acid in fish muscle. 478 fish samples such as olive flounder, genuine porgy, common sea bass and black rock fish collected from fish farms in the coastal area from September 2001 to October 2004 were analyzed to evaluate overall efficiency of the modified method and to monitor the actual condition of oxolinic acid usage in fish farm. According to the monitoring results, the modified method was suitable for analysis of oxolinic acid in fish muscle and oxolinic acid might be used in a small portion of fish farms. The suggested analysis method of oxolinic acid was registered in the Korean Official Methods of Food Analysis and is being utilized for fishery products by the Korea Food and Drug Adminstration and the National Fisheries Products Quality Inspection Service.
Keywords
HPLC; Oxolinic acid;
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  • Reference
1 KQS (Kobe Quarantine Station). 1996. Analysis of synthetic antimicrobials in livestock and seafood products imported. - Rapid determination of residual synthetic antibacterials in fish and meat by HPLC with linear gradient elution. Bull. Kobe Quarant. Station, 1-7
2 Larocque, L., M. Schnurr, S. Sved and A. Weninger. 1991. Determination of oxolinic acid residues in salmon muscle tissue by liquid chromatography with fluorescence detection. J. Assoc. Off. Anal. Chern., 74, 608-611
3 Nose, N., Y. Hashino, Y. Kikuchi, M. Horie, K. Saitoh, T. Kawachi and H. Nakazawa. 1987. Simultaneous liquid chromatographic determination of residual synthetic antibacterials in cultured fish. J. Assoc. Off. Anal. Chern., 70, 714-717
4 Pouliquen, H. and F. Armand. 2000. Determination of oxolinic acid in faeces and turbot (Scophthalmus maximus) by high-performance liquid chromatography using fluorescence detection. J. Chromatogr. B, 749, 127-133   DOI   ScienceOn
5 Ueno, R., M. Okumura, Y. Horiguchi and S.S. Kubota. 1988. Levels of oxolinic acid in cultured rainbow trout and amago salmon after oral administration. Nippon Suisan Gakkaishi, 54, 485-489   DOI
6 Ikai, Y., H. aka, N, Kawamura, M. Yamada, K. Harada, M. Suzuki and H. Nakazawa. 1989. Improvement of chemical analysis of antibiotics: XVI. Simple and rapid determination of residual pyridonecarboxylic acid antibacterials in fish using a prepacked amino cartridge J. Chromatogr. A, 477, 397-406   DOI   ScienceOn
7 Chun, S.K. 1992. Guidance for use of aquaculture medicine. In: Disease of marine farmed fish. Susan Times, Seoul, 126-131
8 Hamamoto, K. 1986. Rapid high-performance liquid chromatographic method for the determination of oxolinic acid in chicken plasma. J. Chromatogr. B, 381, 453-456   DOI   ScienceOn
9 Horii, S., C. Yasuoka and M. Matsumoto. 1987. Highperformance liquid chromatographic method for the simultaneous determination of oxolinic, nalidixic and piromidic acids in cultured fish. J. Chromatogr. A, 388, 459-466   DOI   ScienceOn
10 Hustvedt, S.O., R. Salte and V. Vassik. 1991. Absorption, distribution and elimination of oxolinic acid in Atlantic salmon after various routes of administration. Aquaculture, 95, 193-199   DOI   ScienceOn
11 Inglis, V. and R.H. Richards. 1991. The in vitro susceptibility of Aeromonas salmonicida and other fish pathogenic bacteria to 29 antimicrobial agent. J. Fish Dis., 14, 641-650   DOI
12 Jacobsen, M.D. 1989. Withdrawal times of freshwater rainbow trout after treatment with oxolinic acid, oxytetracycline and trimethoprim. J. Fish Dis. 12, 29-36   DOI   ScienceOn
13 Jeong, H.D. and S.K. Chun. 1992. The utilization of antibiotics and the treatment of bacterial diseases in fish. J. Fish Pathol., 5, 37-48
14 Kasuga, Y., A. Sugitani and F. Yamada. 1982. Simultaneous determination of oxolinic, nalidixic and piromidic acids in fishes by high performance liquid chromatography. J. Food Hyg. Soc. Jpn., 23, 344-347   DOI
15 KAHPA (Korea Animal Health Products Association). 2001. The Guidebook of Veterinary Antibiotics, Kyungsung Munhwasa, GyeongGi-Do, 645-653
16 KFDA (Korea Food and Drug Administration). 2000. Korea Food Code (A supplementary), Chapter 7. General method for examination, Munyoungsa, Seoul, 363-365
17 Kim, K.H., M.R. Song, S.N. Choe, M.S. Choe and K.H. Park. 1998. Oxolinic acid residue in the cultured eel tissues and its change to heating process. J. Fd. Hyg. Safety, 13, 14-19