• Journal of Plant Biotechnology
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• 제36권4호
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• pp.301-308
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• 2009

Higher agricultural commodity prices are a particular concern for food importing countries like Korea that has a very low self-sufficiency ratio. Korean people eat approximately 4.5 million metric tons of rice each year, which is met without a problem by domestic production. The domestic production of corn and soybean which are important raw materials for commercial food processing and livestock feed is only minimal. Demands of corn and soybean in Korea are approximately 7.2 million and 1.3 million metric tons per year, respectively. Since Korean consumers are reluctant to accept biotech (GM) foods, Korean food processors are fighting an up-hill battle in purchasing non-biotech (non- GM) crops which are becoming scarce.

• 한국작물학회지
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• 제49권spc1호
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• pp.195-202
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• 2004

• 농업과학연구
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• 제44권2호
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• pp.188-195
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• 2017

Biotech crops can only be commercialized after they receive safety approvals, which require thorough risk assessments of their release to the environment. Environmental release experiments are indispensable for environmental risk assessments, and each country has been preparing its own regulations for the safety management of experiments on the environmental release of biotech crops in confined fields. In this study, we compared and analyzed the safety management regulations of the environmental release of biotech crops in Korea, USA, Japan, European Union, and China. Each country had safety management regulations for the environmental release of biotech crops, and these regulations were generally not much different from the Korean regulations. However, there was a difference amongst the USA, Japan, and China in regulations for isolation distances to prevent gene diffusion through pollen-flow during environmental release experiments of biotech crops. In order to establish the isolation distance regulation suitable for the Korean environment, relevant data were collected and presented. For setting the isolation distance for environment release of biotech crops, it is suggested to refer to the isolation distance information provided in the Guidance of Seed Management in Korea. The results of this study are expected to help establish the safety management of biotech crops in Korea.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2008년도 춘계학술발표회
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• pp.284-285
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• 2008

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2008년도 춘계학술발표회
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• pp.55-56
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• 2008

• Molecules and Cells
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• 제41권4호
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• pp.342-350
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• 2018

Flowering time is determined by florigens. These genes include, Heading date 3a (Hd3a) and Rice FT 1 (RFT1) in rice, which are specifically expressed in the vascular tissues of leaves at the floral transition stage. To study the cis-regulatory elements present in the promoter region of Hd3a, we generated transgenic plants carrying the 1.75-kb promoter fragment of Hd3a that was fused to the ${\beta}$-glucuronidase (GUS) reporter gene. Plants expressing this construct conferred a vascular cell-specific expression pattern for the reporter gene. However, GUS was expressed in leaves at all developmental stages, including the early seedling stage when Hd3a was not detected. Furthermore, the reporter was expressed in roots at all stages. This suggests that the 1.75-kb region lackings cis-elements that regulate leaf-specific expression at the appropriate developmental stages. Deletion analyses of the promoter region indicated that regulatory elements determining vascular cell-specific expression are present in the 200-bp region between -245 bp and -45 bp from the transcription initiation site. By transforming the Hd3a-GUS construct to rice cultivar 'Taichung 65' which is defective in Ehd1, we observed that Ehd1 is the major regulatory element that controls Hd3a promoter activity.

• Molecules and Cells
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• 제42권5호
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• pp.406-417
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• 2019

RICE FLOWERING LOCUS T 1 (RFT1) is a major florigen that functions to induce reproductive development in the shoot apical meristem (SAM). To further our study of RFT1, we overexpressed the gene and examined the expression patterns of major regulatory genes during floral transition and inflorescence development. Overexpression induced extremely early flowering in the transgenics, and a majority of those calli directly formed spikelets with a few spikelets, thus bypassing normal vegetative development. FRUITFULL (FUL)-clade genes OsMADS14, OsMADS15, and OsMADS18 were highly induced in the RFT1-expressing meristems. OsMADS34 was also induced in the meristems. This indicated that RFT1 promotes the expression of major regulatory genes that are important for inflorescence development. RFT1 overexpression also induced SEPALLATA (SEP)-clade genes OsMADS1, OsMADS5, and OsMADS7 in the greening calli before floral transition occurred. This suggested their possible roles at the early reproductive stages. We found it interesting that expression of OsFD1 as well as OsFD2 and OsFD3 was strongly increased in the RFT1-expressing calli and spikelets. At a low frequency, those calli produced plants with a few leaves that generated a panicle with a small number of spikelets. In the transgenic leaves, the FUL-clade genes and OsMADS34 were induced, but SEP-clade gene expression was not increased. This indicated that OsMADS14, OsMADS15, OsMADS18, and OsMADS34 act immediately downstream of RFT1.

• Journal of Plant Biotechnology
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• 제36권4호
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• pp.327-335
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• 2009

Creeping bentgrass (Agrostis stolonifera L.) is economically important as the principal turfgrass species for golf course greens and fairways in temperate climates around the world. As the utilization area of the turfgrass species increases recently, the demand for new and improved cultivars increases. Thus, substantial progress has been made in applying modern biotechnology to develop genetically engineered (i.e., biotech) creeping bentgrass with new traits that eluded the breeders. This review article addresses the advances made in developing biotech creeping bentgrass, which are categorized in the following topics: (i) genetic transformation of creeping bentgrass, (ii) development of various biotech creeping bentgrasses by genetic engineering, and (iii) progresses in the deregulation of herbicideresistant creeping bentgrass.

• 한국약용작물학회지
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• 제13권3호
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• pp.118-121
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• 2005

한국에서 재배되고 있는 당귀속 (Angelica) 식물 7종을 대상으로 핵형 분석을 수행하여 다음과 같은 결과를 얻었다. 당귀속 식물들의 기본염색체 수는 x=11로, 체세포 염색체 수는 모든 종에서 2n = 2x = 22로 관찰되었다. 참당귀의 핵형은 K(2n) = 2x = 20m + 2sm, 일당귀의 핵형은 K(2n) = 2x = 12m + 10sm, 당당귀의 핵형은 K(2n) = 2x =16m + 6sm, 고본의 핵형은 K(2n) = 2x = 22m, 바디나물은 K(2n) = 2x = 18m + 4sm, 구릿대는 K(2n)= 2x = 10m + 10sm 2st, 왜친궁은 K(2n)= 2x = 22t로 각각 구분되었다. 염색체 크기는 $3.56\;{\mu}M-8.91\;{\mu}M$ 사이였다. 고본의 체세포 염색체는 모두 중부 염색체로, 왜천궁은 모두 차단부 염색체로만 관찰되어 다른 종들과 이질적인 핵형을 보였다. 한국에 본포하고 있는 당귀속 식물의 핵형과 러시아, 일본, 중국에 분포하는 식물들의 핵형과 비교에서는 일부 다형현상이 관찰되었다.

• 한국약용작물학회지
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• 제11권1호
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• pp.24-30
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• 2003

11종의 약리식물의 수용성 추출물에서 세포독성능과 TGEV와 PEDV에 대한 항바이러스 효능을 분석하였다. 이들 추출물은 바이러스의 숙주세포로 사용되는 ST세포와 Vero세포에 대해 다른 세포독성능을 나타내었는데, 오가피 추출물은 TGEV에 대한 숙주세포주인 ST 세포에 대해 75.81 %의 세포독성을 나타내었으며, 화살나무, 무화과나무, 까마종이, 마황, 창이자 추출물은 20-50% 사이의 세포독성을 나타내었다. 그러나 마황 추출물(35.66%) 이외의 10종의 추출물은 PEDV에 대한 숙주세포주인 Vero 세포에 대해 세포독성능을 나타내지 않았다. 세포독성이 낮은 식물추출물에서 초피나무, 구지뽕나무, 누리장나무, 고삼 추출물이 TGEV에 대하여 효과적인 항바이러스능을 나타내었으며, PEDV에 대하여는 초피나무, 조각자나무, 고삼, 까마종이, 마황 추출물에서 효과적인 항바이러스능을 나타내었다. 이중에서 고삼과 초피나무 추출물은 TGEV와 PEDV에 대해서 효과적인 항바이러스능을 나타내었다.