• Journal of the Optical Society of Korea
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• v.14 no.2
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• pp.65-76
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• 2010

Plasmonic-based biosensing technologies have been successfully commercialized and applied for monitoring various biomolecular interactions occurring at a sensor surface. In particular, the recent advances in nanofabrication methods and nanoparticle syntheses provide a new route to overcome the limitations of a conventional surface plasmon resonance biosensor, such as detection limit, sensitivity, selectivity, and throughput. In this paper, optical and physical properties of plasmonic nanostructures and their contributions to a realization of enhanced optical detection platforms are reviewed. Following vast surveys of the exploitation of metallic nanostructures supporting localized field enhancement, we will propose an outlook for future directions associated with a development of new types of plasmonic sensing substrates

• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.87-87
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• 2013

Sample concentration and purification processes are essential in the bio-analytical and pharmaceutical fields because most bio samples or media are extremely sophisticated. To concentrate and purify specific substances, passive membrane type filters have been utilized, which is driven by size or charge differences between target and others. The traditional and representative method to identify nucleic acid sequences in the complex biosample is gel electrophoresis, which has been worked by size and net charge of molecules. The adsorption phenomena have been also utilized to concentrate and purify biomolecules. This adsorption of biomolecule can be controlled under specific salts and surfaces as well as surface area. To utilize the differences of physical properties of molecules or bio-targets such as virus, bacteria, and cells, the nanotechnologies can be introduced in target concentration, purification, and isolation processes. In here, I'd like to briefly survey typical examples of nanobiotechnologies which are introduced in sample treatment. Also I specifically demonstrate two different simple techniques to concentrate and detect bacteria from the samples using multifunctional silica nanotube (SNT).

• Journal of Magnetics
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• v.13 no.1
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• pp.30-33
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• 2008

A highly sensitive, giant magnetoresistance-spin valve (GMR-SV) biosensing device with high linearity and very low hysteresis was fabricated by photolithography. The detection of magnetic nanoparticles and Fe-hemoglobin inside red blood cells using the GMR-SV biosensing device was investigated. When a sensing current of 1 mA was applied to the current electrode in the patterned active devices with an area of $2{\times}6{\mu}m^2$, the output signals were about 13.35 mV. The signal from even one drop of human blood and nanoparticles in distilled water was sufficient for their detection and analysis.

• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.3
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• pp.202-206
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• 2000

The genetically-engineered Escherichia coli strain, DPD2540, which contains a fabA:::luxCDAbefusion gene, gives a bioluminescent output when membrane fatty acid synthesis is needed. For more pactical application of this strain in the filed as biosensor, freezedrying was adopted. A 12% surcrose solution with Luria-Bertani (LB) broth, as determined by the viability after freeze-drying, was found to be most most effective composition for lyophilization solution among various compositions testitons tested. Rapid freezing with liquid nitrogen also gave the best viability after freeze-drying as compared to samples frozen at-7$0^{\circ}C$ and -2$0^{\circ}C$. The biosensing activities of the cells showed a greater sensitivity when the cells from the expontial phase were freeze-dried. Finally, the optimum temperature for use of the freeze-dried cells in the biodencor field was determined.

• Applied Science and Convergence Technology
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• v.25 no.1
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• pp.7-14
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• 2016

Nanoplasmonics is a developing field that offers attractive optical, electrical, and thermal properties for a wide range of potential applications. Based on the compelling characteristics of this field, researchers have shed light on the possibilities of integrated photonics and biosensing platforms using nanoplasmonic principles. Single and unique nanostructures with plasmons can act as individual transducers that convert desired information into measurable and readable signals. In this review, we will discuss nanoplasmonic sensors, especially those in relation to photodetectors for future optical interconnects, and bioinformation sensing platforms based on nanoplasmonics, thus providing a viable approach by which to create sensors corresponding to target applications. In addition, we also discuss scalable fabrication processes for the creation of unconventional nanoplasmonic devices, which will enable next-generation plasmonic devices for wearable, flexible, and biocompatible systems.

• Polymer Science and Technology
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• v.26 no.2
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• pp.126-139
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• 2015

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2012.05a
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• pp.101-102
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• 2012

• Proceedings of the Optical Society of Korea Conference
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• 2008.02a
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• pp.271-272
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• 2008

• Smart Structures and Systems
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• v.8 no.1
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• pp.17-37
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• 2011

The microcantilever (MCL) sensor is one of the most promising platforms for next-generation label-free biosensing applications. It outperforms conventional label-free detection methods in terms of portability and parallelization. In this paper, an overview of recent advances in our understanding of the coupling between biomolecular interactions and MCL responses is given. A dual compact optical MCL sensing platform was built to enable biosensing experiments both in gas-phase environments and in solutions. The thermal bimorph effect was found to be an effective nanomanipulator for the MCL platform calibration. The study of the alkanethiol self-assembly monolayer (SAM) chain length effect revealed that 1-octanethiol ($C_8H_{17}SH$) induced a larger deflection than that from 1-dodecanethiol ($C_{12}H_{25}SH$) in solutions. Using the clinically relevant biomarker C-reactive protein (CRP), we revealed that the analytical sensitivity of the MCL reached a diagnostic level of $1{\sim}500{\mu}g/ml$ within a 7% coefficient of variation. Using grazing incident x-ray diffractometer (GIXRD) analysis, we found that the gold surface was dominated by the (111) crystalline plane. Moreover, using X-ray photoelectron spectroscopy (XPS) analysis, we confirmed that the Au-S covalent bonds occurred in SAM adsorption whereas CRP molecular bindings occurred in protein analysis. First principles density functional theory (DFT) simulations were also used to examine biomolecular adsorption mechanisms. Multiscale modeling was then developed to connect the interactions at the molecular level with the MCL mechanical response. The alkanethiol SAM chain length effect in air was successfully predicted using the multiscale scheme.

• Bulletin of the Korean Chemical Society
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• v.34 no.6
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• pp.1883-1886
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• 2013