• Korean Journal of Clinical Laboratory Science
• /
• v.37 no.2
• /
• pp.65-70
• /
• 2005

Those specifications of the CLIA analytical tolerance limits are consistent with the performance goals in Six Sigma Quality Management. Six sigma analysis determines performance quality from bias and precision statistics. It also shows if the method meets the criteria for the six sigma performance. Performance standards calculates allowable total error from several different criteria. Six sigma means six standard deviations from the target value or mean value and about 3.4 failures per million opportunities for failure. Sigma Quality Level is an indicator of process centering and process variation total error allowable. Tolerance specification is replaced by a Total Error specification, which is a common form of a quality specification for a laboratory test. The CLIA criteria for acceptable performance in proficiency testing events are given in the form of an allowable total error, TEa. Thus there is a published list of TEa specifications for regulated analytes. In terms of TEa, Six Sigma Quality Management sets a precision goal of TEa/6 and an accuracy goal of 1.5 (TEa/6). This concept is based on the proficiency testing specification of target value +/-3s, TEa from reference intervals, biological variation, and peer group median mean surveys. We have found rules to calculate as a fraction of a reference interval and peer group median mean surveys. We studied to develop total error allowable from peer group survey results and CLIA 88 rules in US on 19 items TP, ALB, T.B, ALP, AST, ALT, CL, LD, K, Na, CRE, BUN, T.C, GLU, GGT, CA, phosphorus, UA, TG tests in chematology were follows. Sigma level versus TEa from peer group median mean CV of each item by group mean were assessed by process performance, fitting within six sigma tolerance limits were TP ($6.1{\delta}$/9.3%), ALB ($6.9{\delta}$/11.3%), T.B ($3.4{\delta}$/25.6%), ALP ($6.8{\delta}$/31.5%), AST ($4.5{\delta}$/16.8%), ALT ($1.6{\delta}$/19.3%), CL ($4.6{\delta}$/8.4%), LD ($11.5{\delta}$/20.07%), K ($2.5{\delta}$/0.39mmol/L), Na ($3.6{\delta}$/6.87mmol/L), CRE ($9.9{\delta}$/21.8%), BUN ($4.3{\delta}$/13.3%), UA ($5.9{\delta}$/11.5%), T.C ($2.2{\delta}$/10.7%), GLU ($4.8{\delta}$/10.2%), GGT ($7.5{\delta}$/27.3%), CA ($5.5{\delta}$/0.87mmol/L), IP ($8.5{\delta}$/13.17%), TG ($9.6{\delta}$/17.7%). Peer group survey median CV in Korean External Assessment greater than CLIA criteria were CL (8.45%/5%), BUN (13.3%/9%), CRE (21.8%/15%), T.B (25.6%/20%), and Na (6.87mmol/L/4mmol/L). Peer group survey median CV less than it were as TP (9.3%/10%), AST (16.8%/20%), ALT (19.3%/20%), K (0.39mmol/L/0.5mmol/L), UA (11.5%/17%), Ca (0.87mg/dL1mg/L), TG (17.7%/25%). TEa in 17 items were same one in 14 items with 82.35%. We found out the truth on increasing sigma level due to increased total error allowable, and were sure that the goal of setting total error allowable would affect the evaluation of sigma metrics in the process, if sustaining the same process.

• Microbiology and Biotechnology Letters
• /
• v.39 no.1
• /
• pp.56-62
• /
• 2011

We investigated quality characteristics of Seoktanju (one of the Korean traditional rice wine) which was fermented using five kinds of Korean commercial Nuruks. The purpose of this study was to research what effects on the quality of Seoktanju by using different Nuruks. We analyzed general component such as each mash's temperature change patterns, pH, titrable acidities, reducing sugar contents, volatile acids, and sugar contents during fermentation periods and studied sensory evaluation of produced Seoktanju (10 days). On the whole, temperature change patterns in the each mashes were depend on room temperature. All Seoktanju's pH was reduced rapidly up to three days after first mashing (pH 3.13-3.57) and after that was increased gradually. The end of fermentation pH was pH 3.6-4.05. Mostly, acidities were indicated high(0.59%) and Nuruk-B was showed highest acid value. These results seems to be different as occasion organic acids producing activity depend on the number of yeast, material contents, optimal temperature in the each mashes by fungi and lactic acid bacteria in Nuruks. In reducing sugar contents and sugar contents, Nuruk-C treatment were showed the highest value with 5.36%, $23^{\circ}brix$, respectively and alcohol content was lowest with 8.6%. In the five kinds of reproduced Seoktanju, alcohol content was the highest in the treated Nuruk-A group. Volatile acid value was the highest with 132.6~263.7 ppm at the 3 day after first mashing day but as the fermentation time goes on, it was reduced sharply by 5.25~5.94 ppm. Sensory evaluation was performed with 5 point scale, the Seoktanju using Nuruk-D was presented by 4 point, while Nuruk-A was presented lowest by 2.77 point on overall acceptability.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.28 no.4
• /
• pp.323-330
• /
• 2008

This study was carried out to evaluate the effect of application of cattle manure and chemical fertilizer on dry matter productivity and nutritive values of rye and hairy vetch according to two different sowing methods such as single or mixed-sowed cultivation. Dry matter and TDN yields for rye single culture, and rye and hairy vetch mixed culture were 7.2 and 4.0 ton/ha, and 8.0 and 4.4 ton/ha, respectively. They were significantly (p<0.05) higher than that (4.5 and 2.7 ton/ha) of hairy vetch single culture. Moreover, plots produced by mixed-sowing cultivation had a higher crude protein (CP) as 7.7% than that of only rye plot, and higher TDN and RFV as 55.8 and 79.4%, respectively. Within rye alone plots, completely cattle slurry application produced significantly higher annual DM and TDN yields (7.4 and 4.1 ton/ha) than those of P+K fertilization (7.2 and 3.9 ton/ha) as a chemical fertilizer and non-fertilizer (5.5 and 3.1 ton/ha). Cattle slurry application plot revealed 75.52 and 78.97% of N+P+K fertilization plot for annual DM and TDN, respectively. Within mixed-sowing cultivation with rye and hairy vetch, completely cattle slurry application produced 7.6 ton/ha DM and 4.5 ton/ha TDN, showing 79.50 and 86.77% of N+P+K fertilization plot, respectively. Furthermore, the CP content for cattle slurry plots was 8.5%, which was significantly (p<0.05) higher than those of other plots, and it was also highest in TDN (58.3%) and RFV (86). Overall, rye mixed-sowing with hairy vetch rather than rye alone sowing reduced DM yields, but leaded to enhancement of feed value and nutritive yields. And also, it would be expected that cattle slurry application in comparison with chemical fertilizers might result in reutilization of resources, improved forage quality and, extended harvest period by preventing feed value from rapidly deteriorating.

• Ocean and Polar Research
• /
• v.36 no.1
• /
• pp.39-47
• /
• 2014

The black scraper Thamnaconus modestus was a commercially important fish species in the 1980s, but suddenly its commercial significance decreased in the 1990s mainly due to continuous overfishing. Recently, in order to reverse the depleted stocks of the black scraper and help the species recover, seed production technology has emerged. This has led to the farming of the black scraper in several parts of the southern coast of Korea. Since detailed research on its metabolism in relation to water temperature has been scanty, this was the investigative focus of the present study. The standard metabolism rates of the black scraper (9-10 months old, total length=$22.6{\pm}0.8cm$, wet weight=$140.3{\pm}13.9g$) were measured at seven different water temperature settings (12, 15, 17, 20, 23, 26, $28^{\circ}C$) to understand the relationship between metabolism and water temperature. Relationships between water temperature (WT) and oxygen consumption rate (OCR) were obtained as SOCR (weight-specific oxygen consumption rate) = 0.0117WT - 0.0135 ($r^2=0.9351$) and IOCR (oxygen consumption rate per individual) = 1.8160WT - 5.4007 ($r^2$ = 9428). The $Q_{10}$ (temperature sensitivity), an indicator of the sensitivity of biological function to temperature, was analyzed. In our experiment, when the water temperature increased, the $Q_{10}$ value decreased. The $Q_{10}$ value was 6.27 in waters where the temperature ranged from $12-15^{\circ}C$ and this was much higher than the values obtained in waters where temperatures ranged between (1) $15-23^{\circ}C$ and (2) $23-28^{\circ}C$. Consequently, it was shown that the black scraper is a warm water species and inhabiting waters in the temperature range from $15-28^{\circ}C$ is deemed appropriate.

• Korean Journal of Ichthyology
• /
• v.10 no.1
• /
• pp.98-105
• /
• 1998

The effect of ammonia on survival and growth of the larval rockfish, Sebastes schlegeli was examined by a static renewal bioassay method. The tolerance of larvae to ammonia toxicity was more sensitive at the early larvae, but increased with the development of larvae. In 14 day-old-larvae after parturition, the mortality with treatment of each concentration of ammonia was 5% at control group and 0.0112 $NH_3$mg/l, whereas it was increased up to 27.5% at exposure group of 0.1230 $NH_3$mg/l with higher concentration. Regression equation between ammonium concentration(X) and mortality(Y) was followed; Y=0.516+3.482 X($r^2=0.4737$, P<0.01). The NOEC(no-observable-effect concentration) and LOEC (lowest-observable-effect concentration) to mortality compared to control group were 0.100 $NH_3mg/l$ and 0.1230 $NH_3$mg/l, respectively and chronic value(ChV) which is the geometric mean of the NOEC and LOEC was 0.1110 $NH_3$mg/l. Body length after 7-days exposure in control group, 0.0112 $NH_3$mg/l and 0.1230 $NH_3$mg/l were 7.8325mm, 7.700mm and 7.05mm, respectively. The NOEC, LOEC and chronic value(ChV) were 0.0335 $NH_3$mg/l, 0.0558 $NH_3$mg/l and 0.0432 $NH_3$mg/l, respectively.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.21 no.1
• /
• pp.240-246
• /
• 2020

This study was conducted to develop specific Single Nucleotide Polymorphism (SNP) markers to identify the genetic characteristics and breed of White Hanwoo (WH) using a molecular biological method. SNP genotyping was performed with an Illumina Bovine HD 777K SNP chip using DNA extracted from 48 Hanwoo and 22 WH. The minor allele frequency (MAF) difference of each SNP was calculated and the statistical significance (P-value) of the MAF difference was calculated through Fisher's Exact test (Genotype). SNPs with 100% difference in the MAF difference were selected based on marker selection criteria. The nine SNP markers with genetic differences were selected. The selected markers have different alleles as being Hanwoo- and WH- specific. Therefore, based on these results, it can be concluded that the Hanwoo and WH varieties can be clearly distinguished by using these SNPs. So, the patent of the WH breed identification markers was registered. WH is a breed that shows the characteristics of a Korean native species that is separate from the native Hanwoo. It is expected that genetic characteristics research on the WH can be used to identify the breed and as a knowledge base for enhancing the value of breeding stock.

• Korean Journal of Ecology and Environment
• /
• v.48 no.4
• /
• pp.253-262
• /
• 2015

The sustainability of Cypripedium japonicum, a rare plant designated by the Korea Forest Service, is threatened due to artificial factors such as habitat loss and climate change etc. and internal factors such as changes in biological properties of the habitat etc. but conservation research has not been performed in South Korea. The objective of this study is to establish the species conservation strategies by analyzing the characteristics of their habitats, including: 1) Population characteristics, and 2) habitat analysis of the vegetation and abiotic environments. From April to September, 2014, population characteristics [density (stems $m^{-2}$), flowering rate (%), and leaf area ($cm^2$)] in Cypripedium japonicum habitats such as Chuncheon (CC), Hwacheon (HC), Muju (MJ), and Gwangyang (GY) and vegetation characteristics (plant sociological research and ordination analysis), and abiotic environments [temperature ($^{\circ}C$), relative humidity (%), transmitted light ($mol{\cdot}m^{-2}{\cdot}d^{-1}$) and canopy openness (%)] were measured. Cypripedium japonicum was mainly distributed at elevation 450 to 990 m and 5 to $30^{\circ}$ slope. Slope direction was shown as 0 to $110^{\circ}$. Habitats temperature (mean $18.94^{\circ}C$) was well matched to seasonal changes. Differences among sites showed greater level according to latitude difference. It showed the highest in habitat, GY located in the South. On the other hand, relative humidity (77.38%) didn't show much difference among sites. The average degree of canopy openness was 18.17%. It showed the highest at HC (22.1%) and the lowest at MJ (16.1%). The average degree of transmitted light was $9.1mol{\cdot}m^{-2}{\cdot}d^{-1}$. It showed the highest at CC ($10.6mol{\cdot}m^{-2}{\cdot}d^{-1}$) and the lowest at GY ($6.87mol{\cdot}m^{-2}{\cdot}d^{-1}$). Chlorophyll content showed average 26.12 SPAD. It showed the highest at MJ (30.64 SPAD value) and the lowest at HC (23.69 SPAD value). Leaf area was average $253.35cm^2$. It showed the highest at CC ($281.51cm^2$) and the lowest at HC ($238.23cm^2$).

• Korean Journal of Soil Science and Fertilizer
• /
• v.42 no.1
• /
• pp.53-57
• /
• 2009

Heavy metal pollution may be one of the most serious challenges confront crop production and human health. Therefore, the selection of heavy metal tolerance cultivars which adapted to the contaminated fields will introduced a suitable solution for management this critical environmental risk. The objectives of this research is to assess human health risk using geochemical analyses and exposure assessment of heavy metals in rice cultivars. Risk for inhabitants in the closed mine area was comparatively assessed for As, Cd, Cu and Pb in 10 rice varieties as a major exposure pathway. The average daily dose (ADD) of each heavy metal was estimated by analyzing the exposure pathways to rice and soil. For the non-carcinogenic risk characterization, Hazard Quotient (HQ) and Hazard Index (HI) were calculated using toxicity indices provided by US-EPA IRIS. The different rice varieties revealed a wide range of HI values from 23.6 to 34.3, indicating that all rice varieties have a high potential toxic risk. The DA rice variety showed the lowest HI value while the TB rice variety the highest. The probabilities of cancer risk for As via rice consumption were varied with rice varieties ranging from 2.0E-03 to 3.5E-03 which exceeded the regulatory acceptable risk of 1 in 10,000 set by US-EPA. The DA rice variety also showed the lowest value while the TB rice variety gave the highest value. Our results indicate that risk assessment can be contribute to screen the pollution safe rice cultivars in paddy fields affected by the mining activity.

• Korean Journal of Food Science and Technology
• /
• v.52 no.6
• /
• pp.610-615
• /
• 2020

Edible insects might be used as a means to solve food insecurity caused by population growth. Many studies have investigated the biological activity of insects; however, few studies have investigated the nutritional value of insects. Therefore, the aim of this study was to investigate the nutritional value of Allomyrina dichotoma larva protein (ADP) as a source of protein replacement. In vivo studies were conducted to determine the food efficiency ratio (FER), protein efficiency ratio (PER), and true digestibility (TD) of ADP. Experiments were conducted in 3 groups of 8 animals per group using twenty-four 4-week-old SD rats. The experimental groups included the general diet group (Con), in which 20% of the total Kcal in the diet was composed of casein protein, and the ADP group (ADP), in which 20% of the total Kcal was composed of ADP protein, and a non-protein diet group (NP) to measure the protein (metabolic fecal nitrogen) excreted by metabolic processes in the body. As a result of this experiment, we found that the FERs were 0.52 and 0.41 in the casein protein intake (Con) and ADP groups, respectively, thus showing a significantly lower level in the ADP group. The PERs of ADP and Con were 2.39 and 2.63, respectively. The TD of Con and ADP were 91% and 80%, respectively.

• Journal of Naturopathy
• /
• v.7 no.1
• /
• pp.10-19
• /
• 2018

The purpose of this study was to investigate the distribution and variation of the anion and cation number in the aerosols at 16 sites in the Jeju area. The average value of anion counts was raged from 449.35 ions/cm³ at Jeju city to 3471.25 ions/cm³ in the Chunjiyeon falls. In order, the lowest Jeju-si < hamdeok < 1100 m < farm < gyorae A < saryoni < jeolmul < gyorae B < geomunoreum < halla forest < hallasan garden < seongpanak < dongbaeksan < jeongbang < wonyang, respectively. There was statistically significant difference between the anion counts of the measured values in the order of elevation. The mean value of cation measurements was from 90 ions/cm³ for Cheonjiyeon, to 729.8 ions/cm³ for Halla forest garden, which showed the highest value. In order, the lowest 729.8 ions/cm³ of Cheonjiyeon < 1100 m < dongbaeksan < Jeju-si < saryoni < wonyang < seongpanak < hamdeok < jeongbang < gyorae B < jeolmul < Farm < gyorae A < halla forest < geomun and < halla garden, respectively. The geographically low area and the high area were measured low and difference in the two ions. The differences between the content of the anion, cation and/or altitude were significant each other in the variance analysis. The correlation between the anion and cation content and/or altitude was statistically significant (r=.396, p<.001). In conclusion, the result of showing the temporal distribution and variation of the anion and cation content in the aerosols in Jeju island forests provides important information for healthcare.