• Journal of Korean Biological Nursing Science
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• 제22권4호
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• pp.260-270
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• 2020

Purposes: Sepsis is a critical condition in which nurses should detect clinical manifestations and provide early intervention to prevent unwanted serious conditions in the patients. The initial occurrence and management of sepsis take place in general units, but there is a lack of knowledge in nurses. The purpose of this study was to examine the effects of a case-based sepsis education program and compare the case-based education program with and without smartphone applications. Methods: A quasi-experimental pre-test-post-test design with a control group was used. We provided a case-based education program with and without smartphone applications to the nurses and tested the effects of the program on knowledge, the accuracy of sepsis assessment, and self-efficacy as outcome variables. A total of 60 nurses in general units participated. To test differences in knowledge, the accuracy of sepsis assessment, and self-efficacy regarding sepsis between the groups over time, a mixed-design ANCOVA was used for parametric analysis, and generalized estimating equations (GEE) were used for nonparametric analysis. Results: There were significant differences in knowledge, the accuracy of sepsis assessment, and self-efficacy between the groups and within the groups over time. The intervention groups treated with the case-based education program showed improved outcome variables compared to the control group. There was no difference between case-based education with the smartphone application or without the application. Conclusions: The case-based education improved knowledge, the accuracy of sepsis assessment, and self-efficacy in the care of sepsis by nurses working in the general wards. The results suggest that the case-based education program for nurses was effective and eventually improved patient health outcomes.

• Applied Biological Chemistry
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• 제38권6호
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• pp.490-495
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• 1995

Cellulomonas sp. KL-6 균주가 생산하는 cellulase 중 CMCase나 FPase는 세포외로 다량 분비되나 ${\beta}-glucosidase$는 cell bound form으로 존재하여 배양액에서의 생산은 극히 미약하였다. CMCase와 FPase는 배양 5일째에, ${\beta}-glucosidase$는 배양 4일째에 각각 최대생산을 나타내었다. $CaCO_3(0.1%)$가 첨가된 효소생산 최적조건에서 CMCase는 $82\;unit/m{\ell}$를, FPase는 $80\;unit/m{\ell}$를 그리고 ${\beta}-glucosidase$는 $1.2\;unit/m{\ell}$를 각각 생산하였고 이는 기본배지에서 보다 $60{\sim}70%$의 생산증가를 가져왔다. KL-6 균주는 lignase와 laccase의 생산은 없었다.

• 한국균학회지
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• 제40권4호
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• pp.296-298
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• 2012

본 연구는 야생버섯으로부터 혈관계질환 관련 생리활성을 확인하여 혈관계질환을 위한 기능성식품 개발에 재료로 사용하기 위해 야생버섯 33종의 야생버섯 메탄올 추출물을 제조하여 심혈관계 질환 생리활성을 확인하였다. 긴대밤그물버섯(Boletellus elatus)과 벽돌빛뿌리버섯(Heterobasidion insulasis)이 높은 혈전용해 활성과 트롬빈저해효과, 항산화활성을 나타내 혈관계질환 치료나 예방을 위한 기능성식품 개발에 이용 가능할 것으로 기대된다.

• Journal of Korean Biological Nursing Science
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• 제2권2호
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• pp.38-52
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• 2000

This study was conducted to develop web-based distance learning program of basic nursing physical science which was composed of a multimedia contents such as documents, voices and pictures. This was to advance curriculum of basic nursing physical science based on a proper nursing characteristics related to other major nursing subjects and induce a new and creative method to gain a knowledge. Based on a general learning design model, distance learning program was developed centered on a structure and function of heart physical science in 1999. The stages of development were like this. Firstly, at the analytic stage, specific units and contents for developing multimedia contents were decided and presentation methods for each item were designed and then the evaluation items were developed. Secondly, at the design stage, the most effective design of multimedia construction was selected and interfaces, basic frames, and presentation elements were chosen. Thirdly, at the developmental stage, internet files were designed using Namo web editor 4.0 and this files were modified with Adobe Photoshop.

• 생명과학회지
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• 제32권9호
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• pp.690-697
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• 2022

본 연구는 홍해삼의 유전체를 분석하여 홍해삼의 유전자 마커 개발을 위한 기초 자료로 활용하기 위해 수행되었다. 울릉도_일반과 울릉도_토착으로 microsatellite marker 분석을 실시하였다. 그 결과 dinucleotide repeat 서열이 81.3~81.4%로 가장 많이 차지 되었으며, 반복서열 개수가 증가될수록 감소되는 경향을 보였다. 일반적으로 microsatellite는 5~10 반복수 사이에 집중적으로 존재하였으며, 반복 서열의 크기가 클수록 반복수가 적어지는 양상을 보였다. Di, tri, tetra-nucleotides 반복에서 각각 (AT)₅, (AAT)₅, (AAAT)₅ 등이 가장 높은 것들로 나타났다. (CG), (CCG) 등은 동일 반복 단위의 다른 반복 단위에 비교하여 매우 낮게 관찰되었다. Di-와 tri-nucleotide는 반복수가 각각 35와 32까지 지속적으로 나타난 다음에 비연속적으로 44와 43 반복까지 계수 되었다. Tetra-, penta- 및 hexa-nucleotide는 각각 25, 21 및 14까지 연속적으로 나타났다. 본 분석결과에 따르면 microsatellite는 특이서열반복에 대해 편중되는 경향성을 보이는 것으로 나타났다. 따라서 홍해삼의 microsatellite 분석에서 고유의 반복 서열과 반복수를 유지하는 것으로 추정되므로 향후 연구를 위한 기초 자료로 활용하는 것이 가능할 것으로 판단된다.

• Journal of Microbiology and Biotechnology
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• 제20권1호
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• pp.132-137
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• 2010

The skc gene encoding streptokinase (SK) with a molecular mass of approximately 47.4 kDa was cloned from Streptococcus equisimilis ATCC 9542 and heterologously overexpressed in Streptomyces lividans TK24 and E. coli using various strong promoters. When the promoter for sprT [Streptomyces griseus trypsin (SGT)] was used in the host S. lividans TK24, a 47.4-kDa protein was detected along with a smaller hydrolyzed protein (44 kDa), suggesting that posttranslational hydrolysis had occurred as has been reported in other expression systems. The casein/plasminogen plate assay revealed that the plasmid construct containing the SGT signal peptide was superior to that containing the SK signal peptide in terms of SK production. Maximal production of SK was calculated to be about 0.25 unit/ml of culture broth, a value that was five times higher than that obtained with other expression systems using ermE and tipA promoters in the same host. When the skc gene was expressed in E. coli BL21(${\Delta}DE3$)pLys under the control of the T7 promoter, a relatively large amount of SK was expressed in soluble form without hydrolysis. SK activity in E. coli/pET28a-$T7_pSK_m$ was more than 2 units/ml of culture broth, even though about half of the expressed protein formed an inactive inclusion body.

• Journal of Microbiology
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• 제39권4호
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• pp.305-313
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• 2001

The spr D gene encoding Strptomyces griseus protease D(SGPD); a chymotrypsin-like proteae, was cloned from Strptomyces griseus IFO13350 and sequence. Most of the amino-acid sequence deduced from the nucleotide sequence is idential to that Strptomyces griseus IMRU3499 except that one amino acid has been deleted and Trp 369 has been substituted into Cys369 in the SGPD from S. griseus IFO13350 without affecting the protease activity. The spr D gene was overexpressed in Streptomyce liv-idans TK24 as a heterologous host. Various media with different compositions were also used to max-imize the productivity of SGPD inthe heterologous host. The SGPD productivity was best when the transformant S. lividans TK24 was cultivated in R2YE medium. The relative chymotrypsin activity of the culture broth measured with an artificial chromogenic substrate, N-scuccinyl-ala-ala-pro-phe-p-nitroanilide, was 16 units/ml. A high level of SGPD was also produced in YEME and SAAM medial but it was relatively lower that in R2YE medium and negligible amounts of SGPD were produced in GYE, GAE and Benedict media. The growth of S. lividans reacted the maximum level of cell mass at days 3 and 4 of the culture, but SGPD production started in the stationary phase of cell growth and kept increase in till the 10$^{th}$ day of culture in R2YE and YEME medium, but in GYE media the productivity reached maximum level at 8days of cultivation. The introduction of the spr D gene into S. lividans TK24 triggered biosyntheis of the pigmented antibiotic , actinorhodin, which implies some protease may paly a very improtant role in secondary-metabolite formation in sStreptomyces.

• 대한의생명과학회지
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• 제16권4호
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• pp.271-277
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• 2010

This study was conducted to investigate the biological activities of Areca catechu L. The antioxidative, fibrinolytic, thrombin inhibitory, and ${\alpha}$-glucosidase inhibitory activities of Areca catechu L extracted with hexane, $CHCl_3$, ethyl acetate, butanol, and water were measured. The water fraction showed the highest extraction yield at 3.65% (w/w). The butanol, $CHCl_3$, water, and ethyl acetate fractions showed strong antioxidative activities at 81.6%, 87.1%, 88.0%, and 89.5%, respectively. The fibrinolytic activity was strong only in the ethyl acetate fraction at 0.84 plasmin units/ml. The 100-fold dilution of the water fraction had the strongest thrombin inhibitory activity at 59.2%. The 100-fold dilution of butanol fraction displayed the strongest ${\alpha}$-glucosidase inhibitory activity at 88.6%. In conclusion, the extracts of Areca catechu L hold promise for use in the development of biofunctional foods to prevent cardiovascular diseases.

• 한국수산과학회지
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• 제55권3호
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• pp.338-344
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• 2022

This study evaluated the external form of Rhodeus notatus TSV (transparent-scaled variant) type identified in Korea for the first time and compared it with that of the normal type. The TSV units newly found on Rhodeus notatus are iii10 of dorsal fin and anal fin iii10, which are similar to that in the normal type, considering the observing coefficient of each part's fins. The external form is, transparent enough to see the red gill filament in the operculum, the spiral digestive organ is observed opaquely, and numerous melanophores are stained at the upper part of abdomen. The coloration and scales are different between TSV type and normal type; however, there is no difference in the external form and meristic character.

• Journal of Korean Biological Nursing Science
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• 제15권3호
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• pp.139-146
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• 2013

Purpose: The purpose of this study was to identify knowledge and the Need of pathophysiology, perceived connectivity between knowledge of pathophysiology and clinical practice, and confidence and satisfaction with clinical performance by applying the knowledge to clinical practice, and to analyze the correlation between the knowledge, perceived connectivity, and confidence and satisfaction of clinical nurses. Methods: Subjects consisted of 149 clinical nurses who work at 7 general hospitals and 6 university hospitals located in Seoul, Incheon, and Gyeonggi province in Korea. Those who learned pathophysiology during their undergraduate program were recruited by using a convenient sampling. The data were collected between April 23 and May 24 in 2012. Participants responded to self-administered questionnaires about knowledge and the need of pathophysiology, and the perceived connectivity between knowledge and clinical practice, and confidence and satisfaction with clinical performance by applying knowledge to clinical practice. Results: Mean scores of knowledge and the need of pathophysiology were $2.88{\pm}0.34$ and $2.33{\pm}0.39$ each. Scores of knowledge and the need of 3 units such as fluid and electrolyte imbalance, acid-base imbalance, and alterations in the respiratory system were high among 23 units while that of adolescent disorders was the lowest. The mean score of perceived connectivity between knowledge and clinical practice was $7.32{\pm}1.57$, and those of confidence and satisfaction with clinical performance by applying the knowledge to clinical practice were $7.66{\pm}1.66$ and $7.42{\pm}1.67$ respectively. Knowledge of pathophysiology was positively correlated with the perceived connectivity between knowledge and clinical practice, and confidence, and satisfaction with clinical performance. Conclusion: The results indicate that knowledge and the need of pathophysiology in clinical nurses is above average, and that knowledge of pathophysiology is important in promoting perceived connectivity with clinical practice and increasing confidence and satisfaction with clinical performance by applying knowledge to clinical practice.