• Title/Summary/Keyword: biological sample

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Desmutagenic Activity of Heated Mountain Herb Juices (산채류(山菜類) 가열즙(加熱汁)의 돌연변이 억제 작용에 관(關)한 연구(硏究))

  • Ham, Seung-Shi
    • Applied Biological Chemistry
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    • v.31 no.1
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    • pp.38-45
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    • 1988
  • Potential mutagenicity of ten heated edible mountain herbs were examined with spore recassay, Ames test and DNA breaking test. Samples of edible mountain herbs were prepared with water extraction at $100^{\circ}C$ for 20 minutes. With the rec-assay, no significant mutagengic activity could be obtained from all of the samples, but among the eight of metal ions added to sample solution, $Pb^{2+}$ to R. crispus heated juice, $Zn^{2+}$ to L. fischeri and S. bracycarpa heated juice increased mutagenic activity of the samples. With the Ames test and DNA breaking test, all of the samples did not show mutagenicity. However, breaking action was activated on heated L. fischeri, P. japonicus. A. triphylla and A. tataricus juices in the presence of 25mM $Cu^{2+}$. But heated A. elata, H. aurantiaca, A. triphylla, S. bracycarpa and A. scaber juices were inactivated in the presence of 25mM $Fe^{2+}$. Desmutagenic activities against benzo$({\alpha})$pyrene significantly increased as increasing concentration of the heated edible mountain herb juices.

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Investigation on Biogenic Amines in Plant-based Minor Korean Fermented Foods (소규모 국내생산 식물 발효식품의 바이오제닉아민 잔류특성)

  • Kim, Jin Hyo;Ryu, Sung-Ji;Lee, Ji-Won;Kim, Young-Wan;Hwang, Han-Joon;Kwon, Oh-Kyoung
    • Journal of Applied Biological Chemistry
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    • v.56 no.2
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    • pp.113-117
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    • 2013
  • Ten major residual biogenic amines including toxic histamine and tyramine were investigated in the plant-based minor Korean fermented food. From the analyses of pickled vegetables, fermented vegetable extracts, fermented tea, black garlic and herbal rice wines, more than 100 mg/kg of histamine were found in pickled soy leaf and pickled mulberry leaf, and also over 1,000 mg/kg of total biogenic amines were found in pickled soy leaf. No sample was found over in black garlic, fermented tea, fermented vegetable extracts and herbal rice wine, less than 100 mg/kg of histamine and/or 1,000 mg/kg of total biogenic amines were observed. Interestingly, all the tested rice wines were found to be over 100 mg/kg of agmatine residue.

Evaluation of emotion-based messages designed to motivate Hispanic and Asian parents of early adolescents to engage in calcium-rich food and beverage parenting practices

  • Banna, Jinan Corinne;Reicks, Marla;Gunther, Carolyn;Richards, Rickelle;Bruhn, Christine;Cluskey, Mary;Wong, Siew Sun;Misner, Scottie;Hongu, Nobuko;Johnston, N Paul
    • Nutrition Research and Practice
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    • v.10 no.4
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    • pp.456-463
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    • 2016
  • BACKGROUND/OBJECTIVES: Setting healthful beverage expectations, making calcium-rich foods and beverages (CRF/B) available, and role modeling are parenting practices promoting calcium intake among early adolescents. This study aimed to evaluate emotion-based messages designed to motivate parents of early adolescents to perform these practices. SUBJECTS/METHODS: Emotion-based messages were developed for each parenting practice and tested in 35 parents from 5 states. Findings were used to modify messages and develop a survey administered via Amazon MechanicalTurk to a convenience sample of Asian (n = 166) and Hispanic (n = 184) parents of children 10-13 years. Main outcome measures were message comprehension, motivation, relevance, acceptability, and novelty. Engagement in the parenting practices was also assessed. RESULTS: Message comprehension was acceptable for the majority of parents. Most also agreed that messages were motivational (setting healthful beverage expectations (69.0%), making CRF/B available (67.4%), and role modeling (80.0%)), relevant and acceptable. About 30-50% indicated they had not seen the information before. Many parents indicated they were already engaging in the practices (> 70%). No racial/ethnic differences were observed for responses to messages or engaging in parenting practices. CONCLUSIONS: Results indicate that emotion-based messages designed to motivate parents to engage in parenting practices that promote calcium intake among early adolescents were motivating, relevant, and acceptable.

Solvent Fractionation of Fig Leaves and its Antimicrobial Activity (무화과 잎의 용매분획 및 항미생물 활성)

  • Kang, Seong-Kuk;Chung, Hee-Jong
    • Applied Biological Chemistry
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    • v.38 no.4
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    • pp.289-292
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    • 1995
  • Fig leaves were extracted with methanol and then fractionated with ethyl acetate and various buffers to get active fractions, and their antimicrobial activities in each fraction were determined. Acidic fraction and phenolic fraction of fig leaves showed strong antimicrobial activities, but the basic fraction and neutral fraction did not show any activities. The degree of antimicrobial activities in phenolic fraction against tested bacteria were higher than those in acidic fraction, but these against yeasts and mold were almost equivalent to those in acidic fraction. Especially, phenolic fraction showed the strongest activities against Staphylococcuns aureus and Pseudomonas aeruginosa. Although there were some differences among microorganisms, minimum inhibition concentrations(MIC) in acidic and phenolic fractions against tested microorganisms were 0.05 to 0.1 g eq./ml and 0.01 to 0.25 g eq./ml, on the basis of fresh sample weight, respectively. Minimum lethal concentrations(MLC) in acidic and phenolic fractions were 0.25 to 2.00 g eq./ml and 0.05 to 1.50 g eq./ml, respectively. These supposed the antimicrobial activities in phenolic fraction were generally higher than those in acidic fraction and also phenolic fraction had lower MIC and MLC values than acidic fraction.

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Biological Hydrogen Production By Pre-treatment of Sugar Wastewater Using Acidic or Alkaline Chemicals (산·알칼리 전처리를 통한 제당 폐수의 생물학적 수소생산)

  • Lee, Tae-Jin
    • Journal of Korean Society of Environmental Engineers
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    • v.35 no.1
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    • pp.10-16
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    • 2013
  • Characteristics of biological hydrogen production rate and organic acid under anaerobic fermentation process were investigated with sugar wastewater. Hydrogen production rate was higher with alkaline pre-treatment than acidic pre-treatment, resulting in 70% increment. An adequate supply of the nutrients (N or P) into raw sugar wastewater could increase hydrogen production rate. Carbohydrate degradation of the anaerobic fermentation process was not directly related with hydrogen production. Sugar wastewater with the addition of the nutrients shows 3 times higher B/A ratio than the raw sugar wastewater. B/A ratio of the wastewater with alkaline pre-treatment and nutrients addition was most higher than other samples, showing 4.02 of B/A ratio. Higher B/A ratio shows higher hydrogen production rate at each sample.

Chemical Components and Comparison of Biological Activities on the Fruit of Natural Bogbunja (Rubus coreanus Miquel) (국내산 복분자 열매에 대한 화학적 조성 및 생리활성 비교)

  • 신공식;박필재;부희옥;고정연;한성수
    • Korean Journal of Plant Resources
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    • v.16 no.2
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    • pp.109-117
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    • 2003
  • This study was carried out to investigate biological and antioxidative activities on the fruit of bogbunja (Rubus coreanus Miquel). Total contents of phenolic compounds contents in cultivars ripened fruit and immatured wild-type fruit were 222 and 190mg/g, respectively, Polysaccharide contents of immatured wild-type fruit were the highest value of 320U. For EDA analysis, immatured wild-type fruit showed over 95% in 100$\mu\textrm{g}$/mL of sample concentration, which is the the most effective. Levels of SOD-like activities in immatured and cultivar ripened fruits were 81% and 77%, respectively, For the inhibitory effect on lipid peroxidation, all of bogbunja prepared were similar with those of $\alpha$-tocopherol as control. The inhibition of ACE activities on the water extracts of bogbunja fruit showed over 98%, especially, in immature wild-type and cultivar bogbunja.

Studies on the Indoles in Common Reed. -[Part 1] Indole Compounds Occuring in the Shoot of Common Reed [Phragmites Communis Trin.]- (갈대의 INDOLE 화합물(化合物) 연구(硏究) -[제1보](第一報) 갈대 유아(幼芽)의 Indole 화합물(化合物) 검색(檢索)-)

  • Kim, Y.H.;Lee, C.Y.;Kim, I.S.
    • Applied Biological Chemistry
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    • v.19 no.1
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    • pp.24-30
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    • 1976
  • Rhizomes of the common reed (Phragmites communis Trin.) were incubated for three days in the dark. Methanol extract of the shoots was thin layer chromatographed with several solvent systems and visualized with five reagents. The results may be summarized as follows: 1. Serotonin, tryptophan, and tryptamine were identified by cochromatography with the respective authentic compounds. Bufotenine, N-methylserotonin, and N,N-dimethyltryptamine were tentatively identified by their Rf values and colour reactions. The presence of skatole and gramine was suggested. 2. It was esteemed that the common reed might have an active methylation/hydroxylation system of indole compounds at least for a period of time. 3. The presently devised 'overlap' thin layer chromatographic technique may be a useful tool for the identification of a compound whose Rf value was diverse from that of the authentic one by the interferance containing in a sample material.

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Sports injuries and the changes in physical activity, perceived health state and exercise self-efficacy according to the sports injuries of the elderly who participate in physical activities (신체활동참여 노인의 운동손상과 운동손상유무에 따른 신체활동, 지각된 건강상태 및 운동자기효능감의 차이)

  • Seo, Kyung Hee;Eun, Young;Jeon, Mi Yang
    • Journal of Korean Biological Nursing Science
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    • v.18 no.2
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    • pp.102-109
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    • 2016
  • Purpose: The purpose of this study was to describe sports injuries among elderly people and to compare physical activity, perceived health status and exercise self-efficacy between elderly people who had experienced a sports injury and elderly people who hadn't. Methods: The sample of this study were 100 elderly in Korea, 51 of whom had had a sports injury. The study used questionnaires to gather data. There were 8 demographic factors, 4 factors related to physical activity, perceived health status and exercise self-efficacy of the subjects. Data were analyzed by frequencies, chi-square test, and t-test. Result: Among the sports injured elders, one occurrence of a sport injury was 86.3%. There were more outdoor than indoor sports injuries of elders (66.7%), and sports injuries of elders occurred more in the winter (82.7%). The major cause of the sports injuries was loss of balance. Ankles were frequently damaged from these injuries. The most significant change after the sports injury was reduction of physical activity (60.8%). There were significant differences between sports injured elderly and non-injured elderly for age (${\chi}^2=2.58$, p=.011) and the amount of physical activity (t=1.96, p=.050), but there was no significant difference in perceived health status and exercise self-efficacy. Conclusion: To prevent sports injuries in the elderly means to maintain a medium level of physical activity and to improve their balance.

Relationships between Sleep Quality, Fatigue and Depression on Health Promoting Behavior by Shift-Work Patterns in University Hospital Nurses (교대근무 형태에 따른 대학병원 간호사의 수면 질, 피로, 우울 및 건강증진 행위 간의 관계)

  • Son, Youn-Jung;Park, Young-Rye
    • Journal of Korean Biological Nursing Science
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    • v.13 no.3
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    • pp.229-237
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    • 2011
  • Purpose: The objective of this study was to investigate the relationships between sleep quality, fatigue, depression and health promoting behavior by shift-work patterns in university hospital nurses. Methods: A cross-sectional, descriptive survey was conducted using a convenient sample. The self-administered questionnaires were given to 165 nurses (shift workers 91, non-shift workers 74) employed in two university hospital located in Seoul and Gyeunggi-do. Data were collected from February to March, 2010. Results: The shift workers showed significantly lower sleep quality and health promoting behavior, and higher fatigue than non-shift workers. There were significant correlations between fatigue, depression and health promoting behavior in shift workers. There were significant correlations between sleep quality, fatigue, depression and health promoting behavior in non-shift workers. In multiple linear regression analysis, after controlling for total work experiences, predictor of health promoting behavior in shift workers was only fatigue (${\beta}$=-.29, p<.001). Predictors of health promotion behavior in non-shift workers were depression (${\beta}$=-.37, p=.002) and fatigue (${\beta}$=-.27, p=.023). Conclusion: This study failed to provide a clear explanation why sleep quality does not have a comparable effect on health promotion behaviors of shift-workers as well as non-shift workers. However, future research is needed to determine the various risk factors influencing on health promotion behaviors by which shift-work pattern exacerbates fatigue.

Differences in Temporal Variation of Ground Beetle Assemblages (Coleoptera: Carabidae) between Two Well-Preserved Areas in Mt. Sobaeksan National Park

  • Jung, Jong-Kook;Suk, Sang-Wook;Kim, Byeong-Young;Hong, EuiJeong;Kim, Youngjin;Jeong, Jong-Chul
    • Journal of Forest and Environmental Science
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    • v.33 no.2
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    • pp.122-129
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    • 2017
  • Understanding how future climate conditions will be impact on the biodiversity and species composition is important, because biodiversity becomes more important in environment assessment. To understand the biological changes including diversity and species composition over time (temporal variation within a year), the species diversity and composition of ground beetles were investigated in two well-preserved areas in the Sobaeksan National Park using pitfall traps. In addition, relationships between ground beetles and environmental variables were studied by considering temporal variation. We collected 2,146 ground beetle specimens representing 45 species, and individual-based rarefaction curves indicated that similar species richness was found between Geumseon Valley (GV) and Namcheon Valley (NV). The Bray-Curtis matrix comparisons between study sites were characterized by similar ground beetles sample heterogeneity, while temporal variations in abundance, species richness, and ${\beta}-diversity$ of ground beetles showed rather difference over time according to location of study sites. In GV site, minimum temperature was selected as the best predictor for abundance, species richness, and ${\beta}-diversity$ of ground beetles, while those relationships in NV site were more complicated. In conclusion, our study suggests that understanding the different response of ground beetles to climatic variables related to local habitat conditions is important to predict the effect of climate change on biological communities.