• Food Science and Preservation
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• v.13 no.1
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• pp.83-87
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• 2006

Gastrodia elata Blume is a major imp0l1ant medicinal resource in Korea. In order to confirm the biological activities of Gastrodia elata Blume, we carried out various in vitro assays. Of them, anti-oxidant and anti-tumor activities were detected from assays. The prototype of Gastrodia elata Blume extracts was used for 1he evaluation of DPPH, FRAP, hydroxyradical scavenging assay as anti-oxidant assays, as well as anti-tumor asctivities as wound assay and invasion assay. As a result, the prototype of Gastrodia elata Blume extracts showed potent anti-oxidative activity and anti-tumor activity in vitro. These above results suggest that 1he Gastrodia elata Blume extracts could have potential to alleviate oxidation process, cell motility activity, and tumorigenesis.

• Molecules and Cells
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• v.45 no.6
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• pp.388-402
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• 2022

Malignant meningiomas often show invasive growth that makes complete tumor resection challenging, and they are more prone to recur after radical resection. Invasive meningioma associated transcript 1 (IMAT1) is a long noncoding RNA located on Homo sapiens chromosome 17 that was identified by our team based on absolute expression differences in invasive and non-invasive meningiomas. Our studies indicated that IMAT1 was highly expressed in invasive meningiomas compared with non-invasive meningiomas. In vitro studies showed that IMAT1 promoted meningioma cell invasion through the inactivation of the Krüppel-like factor 4 (KLF4)/hsa-miR22-3p/Snai1 pathway by acting as a sponge for hsa-miR22-3p, and IMAT1 knockdown effectively restored the tumor suppressive properties of KLF4 by preserving its tumor suppressor pathway. In vivo experiments confirmed that IMAT1 silencing could significantly inhibit the growth of subcutaneous tumors and prolong the survival period of tumor-bearing mice. Our findings demonstrated that the high expression of IMAT1 is the inherent reason for the loss of the tumor suppressive properties of KLF4 during meningioma progression. Therefore, we believe that IMAT1 may be a potential biological marker and treatment target for meningiomas.

• The Korean Journal of Pain
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• v.36 no.1
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• pp.60-71
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• 2023

Background: The purpose of this research was to assess the role of heparanase (HPSE)/syndecan1 (SDC1)/nerve growth factor (NGF) on cancer pain from melanoma. Methods: The influence of HPSE on the biological function of melanoma cells and cancer pain in a mouse model was evaluated. Immunohistochemical staining was used to analyze HPSE and SDC1. HPSE, NGF, and SDC1 were detected using western blot. Inflammatory factors were detected using ELISA assay. Results: HPSE promoted melanoma cell viability, proliferation, migration, invasion, and tumor growth, as well as cancer pain, while SST0001 treatment reversed the promoting effect of HPSE. HPSE up-regulated NGF, and NGF feedback promoted HPSE. High expression of NGF reversed the inhibitory effect of HPSE down-regulation on melanoma cell phenotype deterioration, including cell viability, proliferation, migration, and invasion. SST0001 down-regulated SDC1 expression. SDC1 reversed the inhibitory effect of SST0001 on cancer pain. Conclusions: The results showed that HPSE promoted melanoma development and cancer pain by interacting with NGF/SDC1. It provides new insights to better understand the role of HPSE in melanoma and also provides a new direction for cancer pain treatment.

• Journal of Microbiology and Biotechnology
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• v.24 no.2
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• pp.287-294
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• 2014

The transcription factor E2F1 is active during G1 to S transition and is involved in the cell cycle and progression. A recent study reported that increased E2F1 is associated with DNA damage and tumor development in several tissues using transgenic models. Here, we show that E2F1 expression is regulated by tristetraprolin (TTP) in prostate cancer. Overexpression of TTP decreased the stability of E2F1 mRNA and the expression level of E2F1. In contrast, inhibition of TTP using siRNA increased the E2F1 expression. E2F1 mRNA contains three AREs within the 3'UTR, and TTP destabilized a luciferase mRNA that contained the E2F1 mRNA 3'UTR. Analyses of point mutants of the E2F1 mRNA 3'UTR demonstrated that ARE2 was mostly responsible for the TTP-mediated destabilization of E2F1 mRNA. RNA EMSA revealed that TTP binds directly to the E2F1 mRNA 3'UTR of ARE2. Moreover, treatment with siRNA against TTP increased the proliferation of PC3 human prostate cancer cells. Taken together, these results demonstrate that E2F1 mRNA is a physiological target of TTP and suggests that TTP controls proliferation as well as migration and invasion through the regulation of E2F1 mRNA stability.

• KSBB Journal
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• v.24 no.5
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• pp.425-431
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• 2009

Hepatocyte growth factor (HGF) and its receptor play an important role in the formation and progression of glioma. In this study, I investigated the ability of HGF to recover of the PSA siRNA-suppressed cell proliferation, migration and invasion in U-251-MG cells. PSA siRNA-transfected U-251-MG cells showed the reduction of the proliferation, migration and invasion with compared to control. Treatment of HGF on the PSA siRNA-transfected U-251-MG cells recovered the ability of proliferation, migration and invasion. These data suggest that PSA and HGF may use unique and parallel signaling cascade leading to the proliferative, migrative and invasive phenotype of U-251-MG cells. I also showed that PSA cooperated with HGF to a migrative and invasive phenotype via the increased secretion of matrix metalloproteinase-2 (MMP-2) and MMP-9.

• Journal of Environmental Impact Assessment
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• v.25 no.3
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• pp.190-208
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• 2016

This study was carried out to understand the spatial distribution and conservation, restoration plan for vegetation in the Hwaeom wetland protected area. Total 29 phytosociological releves consisted of 117 plant species (unidentified species contained) was made during field studies conducted at 2008 and 2013 applying Braun-Branquet method. The collected vegetations were arranged 8 plant communities and thus divided hydric types (Drosera rotundifolia-Eleocharis congesta community, Carex forficula-Molinia japonica community) and xeric types (Miscanthus sinensis community, Rhododendron yedoense var. poukhanense community, Tripterygium regelii community, Carex gifuensis-Quercus mongolica community) under moisture conditions. The hydric types growing in hydric conditions distributed along hollowed out ground or the water channel that have a key qualification to protect. In the wetland protected area, the hydric vegetation types were mostly covered about 3.8% and the xeric types dominating by Miscanthus sinensis were most wildly distributed about 51.5%. Various woody plant species invaded the place. In 2008, invasion plants observed with total 14 species - 8 tree species (227 individual), 6 shrub species (51 individual) and alders (Aluns japonica) found in 2013. We suggested finding out that the sustainable conservation and management in Hwaeom wetland should be setting up plan focused on activity and variability such as continuous monitoring (changing vegetation types, spatial distribution, invasion plants) and zonation management (core zone, buffer zone, transition zone).

• Ecology and Resilient Infrastructure
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• v.2 no.3
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• pp.191-197
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• 2015

Invasive alien species are considered to be one of the main factors that cause biodiversity loss. Establishment of management strategies through continuous monitoring and risk assessment is a key element for invasive alien species management policy. In the present study, we introduce examples of ecological risk assessment tools developed in Japan, Germany-Austria and Belgium. Invasive alien species have been designated in Japan based on the assessment of risks to ecosystems, human health and primary industry. German-Austrian Black List Information System categorized alien species into Black List, White List and Grey List according to their risks to biodiversity. In the Harmonia Information System developed in Belgium, invasiveness, adverse impacts on native species and ecosystem functions and invasion stages were assessed and alien species were categorized into Black List, Watch List and Alert List. These international risk assessment tools may be helpful to improve our national risk assessment protocol for the prioritization of invasive alien species management.

• Ecology and Resilient Infrastructure
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• v.2 no.3
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• pp.255-263
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• 2015

Biological invasion of alien plants is considered to be one of the most serious threats to biodiversity in riparian zones. The effects of two invasive alien plants, Sicyos angulatus and Paspalum distichum var. indutum, on the flora and community structure of the riparian vegetation were investigated at 22 sites at streams in Korea. Sicyos angulatus has invaded the central Korean Peninsula. This alien plant has caused problems to stream managers because of its aggressive vining growth. It had suppressed native vegetation such as trees, shrubs and tall grasses on bank slope and higher floodplains. Paspalum distichum var. indutum has become more widespread in the southern part of Korea. This invasive plant has shallow rhizomes and creeping, extensively branched stolons. It forms a dense mat over lotic or slowly-flowing water and threatens submerged and short emergent hydrophytes. In order to control the introduction and expansion of alien plants, limitation of artificial disturbances and appropriate alien plant management are needed in riparian areas.

• Korean Journal of Environmental Biology
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• v.41 no.2
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• pp.138-144
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• 2023

The main factor of biodiversity decline in major biological populations around the world is invasion of alien species. To protect native species, it is necessary to manage alien species. Recently, to eradicate ecosystem disturbance caused by alien species in Korea, many efforts have been made to capture individuals using nets and purchase captured individuals. However, there is no standard for classifying species due to the form of nest site or external characteristics of eggs of freshwater turtles. Thus, Mauremys reevesii eggs might be discarded due to mistaking as eggs of alien turtles. Based on more data, this study aims to compare and analyze external differences among eggs of Trachemys scripta elegans, Pseudemys concinna, and M. reevesii and use them as reference materials in the process of eradicating alien turtles. This study measured characteristics of eggs of the three turtle species. As a result of comparison, all variables of external characteristics of alien turtles and M. reevesii eggs showed significant differences. The shape of egg was also different, with eggs of T. scripta elegans and P. concinna showing a bicone shape and those of M. reevesii showing an ellipsoid shape. In conclusion, based on results of previous studies and the present study, eggs of M. reevesii, T. scripta elegans, and P. concinna are different in shape and structure. Thus, it is possible to distinguish between M. reevesii and invasive alien turtle using their eggs.

• Journal of the Korean Institute of Intelligent Systems
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• v.11 no.4
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• pp.320-324
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• 2001

According to the rapid growth of computer and internet recently, A hacking to steal infonnations and the computer vinls to destroy the data in computer are now prevailing in the whole world. A study of methods to protect the data of computer is in progress. One of the study is constmction of computer immune system using biological immune system tbat has ability of removal and protection from extemal invasion. In this paper, we make a change detection algorithm which is based on ability of distinction between self and nonself in T-cytotoxic cell that is one of biological immune cell. In algorithm, MHC receptors are composed of a part of self-file that is recognized as itself and those shall distinguish self-file from the changed file. As a result of applying this algorithm to the changed self-files, we prove the efficacy of detection of the self-files changed by computer virus and hacking.