• 한국균학회지
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• 제50권4호
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• pp.347-355
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• 2022

A fungal isolate belonging to the phylum Ascomycota was isolated and identified as Talaromyces veerkampii in 2017 during a survey of fungal diversity in field soils in Korea. This fungal isolate was identified and described based on macro- and micromorphological and molecular characterization. The identification was also based on partial 18S-ITS1-5.8S-ITS2-28S rDNA and calmodulin (CaM)-encoding gene sequencing data. Talaromyces veerkampii has not been previously reported in Korea. Thus, we report here a newly discovered species from soil in Korea along with its morphological and molecular characteristics.

• Journal of Ecology and Environment
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• 제46권4호
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• pp.292-303
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• 2022

Background: Climate change significantly influences the geographical distribution of plant species worldwide. Selecting indicator species allows for better-informed and more effective ecosystem management in response to climate change. The Korean Peninsula is the northernmost distribution zone of warm temperate evergreen broad-leaved (WTEB) species in Northeast Asia. Considering the ecological value of these species, we evaluated the current distribution range and future suitable habitat for 13 WTEB tree species designated as climate-sensitive biological indicator species. Results: Up-to-date and accurate WTEB species distribution maps were constructed using herbarium specimens and citizen science data from the Korea Biodiversity Observation Network. Current northern limits for several species have shifted to higher latitudes compared to previous records. For example, the northern latitude limit for Stauntonia hexaphylla is higher (37° 02' N, Deokjeokdo archipelago) than that reported previously (36° 13' N). The minimum temperature of the coldest month (Bio6) is the major factor influencing species distribution. Under future climate change scenarios, suitable habitats are predicted to expand toward higher latitudes inland and along the western coastal areas. Conclusions: Our results support the suitability of WTEB trees as significant biological indicators of species' responses to warming. The findings also suggest the need for consistent monitoring of species distribution shifts. This study provides an important baseline dataset for future monitoring and management of indicator species' responses to changing climate conditions in South Korea.

• 한국환경복원기술학회지
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• 제25권2호
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• pp.55-67
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• 2022

Land use is a critical factor that affects the hydrological characteristics of watersheds, thereby determining the biological condition of streams. This study analyzes the effects of land uses in the watersheds on biological indicators of streams across the Han River basin using a linear model (LM) and generalized additive model (GAM). LULC and biological monitoring data of streams were obtained from the Korean Ministry of Environment. The proportions of urban, agricultural, and forest areas in the watersheds were regressed to the three biological indicators, including diatom, benthic macroinvertebrate, and fish of streams. The estimated LM and GAM models for the biological indicators were then compared, using regression determination R² and AIC values. The results revealed that GAM models performed better than the LM models in explaining the variances of biological indicators of streams, indicating the non-linear relationships between biological indicators and land uses in watersheds. Also, the results suggested that the indicator of macroinvertebrates was the most sensitive indicator to land uses in watersheds. Although non-linear relationships between watershed land uses and biological indicators of streams could vary among biological indicators, it was consistent that streams' biological integrity significantly deteriorated by a relatively low percentage of urban areas. Meanwhile, biological indicators of streams were negatively affected by the relatively high percentage of agricultural areas. The results of this study can be integrated into effective quantitative criteria for the watershed management and land use plans to enhance the biological integrity of streams. In specific, land uses management plans in watersheds may need more close attention to urban land use changes than agricultural land uses to sustain the biological integrity of streams.

• Journal of Microbiology and Biotechnology
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• 제31권6호
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• pp.890-901
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• 2021

The 11α-hydroxylation of canrenone can be catalyzed by Aspergillus ochraceus in bioreactors, where the geometry of the impeller greatly influences the biotransformation. In this study, the effects of the blade number and impeller diameter of a Rushton turbine on the 11α-hydroxylation of canrenone were considered. The results of fermentation experiments using a 50 mm four-blade impeller showed that 3.40% and 11.43% increases in the conversion ratio were achieved by increasing the blade number and impeller diameter, respectively. However, with an impeller diameter of 60 mm, the conversion ratio with a six-blade impeller was 14.42% lower than that with a four-blade impeller. Data from cold model experiments with a large-diameter six-blade impeller indicated that the serious leakage of inclusions and a 22.08% enzyme activity retention led to a low conversion ratio. Numerical simulations suggested that there was good gas distribution and high fluid flow velocity when the fluid was stirred by large-diameter impellers, resulting in a high dissolved oxygen content and good bulk circulation, which positively affected hyphal growth and metabolism. However, a large-diameter six-blade impeller created overly high shear compared to a large-diameter four-blade impeller, thereby decreasing the conversion ratio. The average shear rates of the former and latter cases were 43.25 s^-1 and 35.31 s^-1, respectively. We therefore concluded that appropriate shear should be applied in the 11α-hydroxylation of canrenone. Overall, this study provides basic data for the scaled-up production of 11α-hydroxycanrenone.

• 한국콘텐츠학회논문지
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• 제18권12호
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• pp.611-623
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• 2018

본 연구는 중년기 여성의 친부모 부양경험에 관한 연구로써 친부모를 부양하는 중년 여성 10명을 대상으로 연구하였다. 그리고 질적연구 방법의 하나로 심층 면담을 통해 자료를 수집하여 Colaizzi의 현상학적 방법으로 분석하였다. 수집된 모든 자료들에서 총 244개의 문장을 추출하였고, 그 중에서 67개의 의미 있는 문장들을 도출하였다. 그리고 그 의미 있는 문장들을 9개의 하위주제로 범주화 하였고, 이어서 4개의 상위 주제를 도출하였다. 연구 결과로는 첫째, 부양에 대한 이유와 동기로는 어쩔 수 없는 상황에서 부양하게 되었고, 그리고 자녀로서의 당연한 의무감과 책임감을 들 수 있었다. 둘째, 부양경험에서 겪는 심리적 현상으로는 부양부담으로 인한 무거운 마음을 감수하고 후회하지 않도록 부모 곁을 지켜드리는 모습으로 나타났다. 셋째, 부양경험의 의미로는 부모님을 평생의 선물처럼 여기고 서로를 향한 마음이 깊어지는 긍정적 요소로 작용하고 있었다. 끝으로, 부양경험의 영향으로는 부모에 대한 이해 증가와 행복에 대한 깨달음 그리고 현재 삶에 대한 성찰과 미래 삶에 대한 조망 등의 결과를 얻게 되었다.

• The Plant Pathology Journal
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• 제37권6호
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• pp.521-532
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• 2021

Knowledge and better understanding of functions of the microbial community are pivotal for crop management. This study was conducted to study bacterial structures including Acidovorax species community structures and diversity from the watermelon cultivated soils in different regions of South Korea. In this study, soil samples were collected from watermelon cultivation areas from various places of South Korea and microbiome analysis was performed to analyze bacterial communities including Acidovorax species community. Next generation sequencing (NGS) was performed by extracting genomic DNA from 92 soil samples from 8 different provinces using a fast genomic DNA extraction kit. NGS data analysis results revealed that, total, 39,367 operational taxonomic unit (OTU), were obtained. NGS data results revealed that, most dominant phylum in all the soil samples was Proteobacteria (37.3%). In addition, most abundant genus was Acidobacterium (1.8%) in all the samples. In order to analyze species diversity among the collected soil samples, OTUs, community diversity, and Shannon index were measured. Shannon (9.297) and inverse Simpson (0.996) were found to have the highest diversity scores in the greenhouse soil sample of Gyeonggi-do province (GG4). Results from NGS sequencing suggest that, most of the soil samples consists of similar trend of bacterial community and diversity. Environmental factors play a key role in shaping the bacterial community and diversity. In order to address this statement, further correlation analysis between soil physical and chemical parameters with dominant bacterial community will be carried out to observe their interactions.

• 미생물과산업
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• 제15권1호
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• pp.29-34
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• 1989

It is our common understanding that biological materials like microorganisms, tissue and cell cultures, seeds, plants and animals are inevitable resources for the development of science and technology. Culture collections which are reservior of biological materials now occupy a central position in life sciences and biotechnology. The World Data Center of Microorganisms (WDC) is a infrastructure of culture collections in the world realizing quick and smooth exchanges of information and microorganisms to support research and development in those fields. The WDC was relocated from University of Queensland in Australia to out institute RIKEN in 1986. This article introduces a function for WDC in RIKEN (RIKAGAKU-KENKYUUSHO, the Institute of Physical and Chemical Research)

• Genomics & Informatics
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• 제7권4호
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• pp.212-216
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• 2009

WebChemDB is an integrated chemical database retrieval system that provides access to over 8 million publicly available chemical structures, including related information on their biological activities and direct links to other public chemical resources, such as PubChem, ChEBI, and DrugBank. The data are publicly available over the web, using two-dimensional (2D) and three-dimensional (3D) structure retrieval systems with various filters and molecular descriptors. The web services API also provides researchers with functionalities to programmatically manipulate, search, and analyze the data.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2000년도 추계종합학술대회
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• pp.328-332
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• 2000

최근 지놈(Genome) 프로젝트를 통해 DNA 염기서열에 대한 정보가 밝혀짐에 따라 분자 수준의 유전자 정보를 다루는 기법이 활발히 연구되고 있다. 그리고 밝혀진 서열정보들의 방대함으로 미루어볼 때 이들 정보를 데이터베이스화하고 효과적인 분석을 행하기 위한 새로운 컴퓨터의 알고리즘의 개발 또한 시급한 일이다. 이러한 측면에서 ,본 논문에서는 분자생물학에서 매우 중요한 연구 대상으로 삼고있는 프로모터 서열과 유전자간의 연관성으로 발현되는 특징을 알아내기 위한 연관 규칙 탐사 알고리즘을 연구한다. 기존의 탐사 알고리즘은 트랜잭션 데이터를 대상으로 하지만 본 논문에서는 생물학적 데이터를 대상으로 하였기 때문에 데이터의 형태와 생물학적인 특성을 수용하는 변형된 연관규칙 알고리즘을 설계한다. 본 연구를 통하여 얻어진 결과는 실제 생물학적 실험'대상의 후보조합을 최소화 하므로써 많은 시간과 노력 비용을 절감할 수 있다.

• ALGAE
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• 제21권1호
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• pp.1-10
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• 2006

Genomic data is accumulating in public database at an unprecedented rate. Although presently dominated by the sequences of metazoan, plant, parasitic, and picoeukaryotic taxa, both expressed sequence tag (EST) and complete genomes of free-living algae are also slowly appearing. This wealth of information offers the opportunity to clarify many long-standing issues in algal and plant evolution such as the contribution of the plastid endosymbiont to nuclear genome evolution using the tools of comparative genomics and multi-gene phylogenetics. A particularly powerful approach for the automated analysis of genome data from multiple taxa is termed phylogenomics. Phylogenomics is the convergence of genomics science (the study of the function and structure of genes and genomes) and molecular phylogenetics (the study of the hierarchical evolutionary relationships among organisms, their genes and genomes). The use of phylogenetics to drive comparative genome analyses has facilitated the reconstruction of the evolutionary history of genes, gene families, and organisms. Here we survey the available genome data, introduce phylogenomic pipelines, and review some initial results of phylogenomic analyses of algal genome data.