• 제목/요약/키워드: biological aging

검색결과 462건 처리시간 0.028초

유카(Yucca shidigera)추출물의 첨가가 Bacillus subtilis p01을 이용한 청국장의 품질 특성에 미치는 영향 (Effect of Yucca (Yucca shidigera) Extract on Quality Characteristics of Chungkookjang using Bacillus subtilis p01)

  • 인재평;이시경
    • Applied Biological Chemistry
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    • 제47권2호
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    • pp.176-181
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    • 2004
  • Bacillus subtilis p01 균주를 사용한 청국장 제조 시 유카 추출물의 첨가가 청국장의 숙성중 품질 특성에 미치는 영향을 검토하고자 청국장 숙성 과정중의 아미노태 질소, 암모니아태 질소, amylase 활성, pretense 활성, 유기산 성분의 변화, 향기성분의 변화를 조사하였다. 청국장의 숙성 중 아미노태질소 함량은 유카 첨가구에서 증가 하였으며, 암모니아태 질소는 유카 첨가구에서 감소하였다. Amylase 활성은 유카 첨가구들이 대조구와 비교 시 높게 나타났으며, 유카 추출물을 0.5 mg/g 첨가 시 효소활성이 가장 높았다. Pretense 활성 역시 유카 추출물 첨가구가 무첨가구보다 다소 높게 나타났다. 유기산은 유카 첨가구들에서 citric acid, acetic acid, malic acid가 검출되었고, 2,5-Dimethylpyrazine이 증가하였고, 불쾌취로 작용되는 cis-3-hexane이 숙성 기간 중 감소함을 보였다.

Insight from sirtuins interactome: topological prominence and multifaceted roles of SIRT1 in modulating immunity, aging, and cancer

  • Nur Diyana Zulkifli;Nurulisa Zulkifle
    • Genomics & Informatics
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    • 제21권2호
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    • pp.23.1-23.9
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    • 2023
  • The mammalian sirtuin family, consisting of SIRT1-SIRT7, plays a vital role in various biological processes, including cancer, diabetes, neurodegeneration, cardiovascular disease, cellular metabolism, and cellular homeostasis maintenance. Due to their involvement in these biological processes, modulating sirtuin activity seems promising to impact immuneand aging-related diseases, as well as cancer pathways. However, more understanding is required regarding the safety and efficacy of sirtuin-targeted therapies due to the complex regulatory mechanisms that govern their activity, particularly in the context of multiple targets. In this study, the interaction landscape of the sirtuin family was analyzed using a systems biology approach. A sirtuin protein-protein interaction network was built using the Cytoscape platform and analyzed using the NetworkAnalyzer and stringApp plugins. The result revealed the sirtuin family's association with numerous proteins that play diverse roles, suggesting a complex interplay between sirtuins and other proteins. Based on network topological and functional analysis, SIRT1 was identified as the most prominent among sirtuin family members, demonstrating that 25 of its protein partners are involved in cancer, 22 in innate immune response, and 29 in aging, with some being linked to a combination of two or more pathways. This study lays the foundation for the development of novel therapies that can target sirtuins with precision and efficacy. By illustrating the various interactions among the proteins in the sirtuin family, we have revealed the multifaceted roles of SIRT1 and provided a framework for their possible roles to be precisely understood, manipulated, and translated into therapeutics in the future.

Effects of Aging Period Prior to Freezing on Meat Quality of Hanwoo Muscle (Longissimus dorsi)

  • Kim, Hyun-Wook;Lee, Eui-Soo;Choi, Yun-Sang;Choi, Ji-Hun;Han, Doo-Jeong;Kim, Hack-Youn;Song, Dong-Heon;Choi, Seul-Gi;Kim, Cheon-Jei
    • 한국축산식품학회지
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    • 제31권6호
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    • pp.799-806
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    • 2011
  • This study was conducted to evaluate the effect of the aging period prior to freezing on the meat quality of Hanwoo longissimus dorsi (LD) muscle. Three different combinations of aging and freezing periods (0/90, 20/70, and 40/50) were examined using LD muscle at 24 h postmortem under an identical storage time of 90 d. The pH and lightness slightly increased with increasing aging period. However, there were no significant (p>0.05) differences in redness and yellowness. The solitary freezing treatment (0/90) had the significantly (p<0.05) lowest moisture content. The un-aged treatment had a significantly (p<0.05) higher total loss than the aged treatments due to an increase in thaw drip loss. The aging significantly improved the myofibrillar fragmentation index and shear force of Hanwoo LD muscle (p<0.05). In addition, the aged treatments produced a higher flavor, tenderness, juiciness, and overall acceptability relative to un-aged treatment. However, there was no significant (p>0.05) difference in shear force and sensorial properties between 20 and 40 d aging prior to freezing. Therefore, 20 d aging prior to freezing may be a sufficiently effective strategy to improve the tenderness and sensorial properties of Hanwoo LD muscle.

꿀풀과 6개종의 Chloroplast 부위 유전자를 이용한 익모초(益母草) 감별 PCR 분석 (PCR Analysis for the Discrimination of Leonuri Herba Medicine on the Basis of Chloroplast DNA Sequence Comparison in Six Lamiaceae Species)

  • 이재웅;김영화;최고야;고병섭;김영선;채성욱;이혜원;오승은;박상언;이미영
    • 대한본초학회지
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    • 제26권3호
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    • pp.15-21
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    • 2011
  • Objectives : The application of polymerase chain reaction (PCR) for the discrimination of the herbal medicine Leonuri Herba (Leonurus japonicus) was evaluated by the comparison of the DNA sequence with Lamiaceae herbal medicine. Method : Genetic analysis showed that phylogenetic tree and comparing sequences through the DNA analysis of rbcL (ribulose-1, 5-bisphosphatecarboxylase) region and trnL-F (tRNA-Leu, trnL-trnF intergeni cspacer, and tRNA-Phe) region of chloroplast DNA from six Lamiaceae sold in market. And we developed IMCF and IMCR primers in order to distinction Leonuri Herba in six Lamiaceae using rbcL and trnL-F sequences. Results : Genetic analysis showed that six Lamiaceae showed individual group on phylogenetic tree. PCR amplification product of Leonuri Herba and another five Lamiaceae were developed for amplification of a 281 bp sequence and the specific PCR amplification of a 460 bp sequence that was exclusive to Leonuri Herba was designed using IMCF and IMCR primers. Conclusion : PCR analysis based on the chloroplast DNA sequences allows the discrimination of Leonuri Herba-based medicine.

만성 폐쇄성 폐질환의 새로운 병인 (New Paradigms in the Pathogenesis of Chronic Obstructive Pulmonary Disease)

  • 김휘정
    • Tuberculosis and Respiratory Diseases
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    • 제69권5호
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    • pp.323-330
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    • 2010
  • A key mechanism in the pathogenesis of chronic obstructive pulmonary disease is thought to be an abnormal inflammatory response in the lungs to the inhalation of toxic particles and gases, derived from tobacco smoke, air pollution, and/or occupational exposures. This review highlights the potential participation of several alternative pathogenetic processes, particularly involving the potential participation of biological and pathobiological processes related to aging, including oxidative stress and enhanced expression of markers of senescence/aging in emphysematous lungs, and the potential for enhanced tissue destruction involving alveolar cell apoptosis.

Longevity regulation by NMD-mediated mRNA quality control

  • Son, Heehwa G.;Lee, Seung-Jae V.
    • BMB Reports
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    • 제50권4호
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    • pp.160-161
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    • 2017
  • Proper maintenance of biological components is crucial for longevity and healthy aging. Although the role of homeostatic maintenance systems for DNA and protein in longevity is established, it remains largely unknown for RNA. In our recent work, we show that nonsense-mediated mRNA decay (NMD) promotes longevity in the roundworm C. elegans by enhancing RNA quality control. We find that the activity of NMD decreases during aging, raising the possibility that RNA quality declines in old animals. We then show that key components of NMD complex are required for prolonged lifespan in C. elegans. In addition, animals with reduced insulin/insulin-like growth factor-1 (IGF-1) signaling (IIS), a representative longevity model, display increased NMD activity. Thus, up-regulation of NMD appears to play crucial roles in longevity conferred by reduced IIS via enhancing mRNA quality control. As both IIS and NMD pathways are evolutionarily conserved, mammals including humans may be equipped with similar RNA quality control systems to achieve longevity.

AMPK Alchemy: Therapeutic Potentials in Allergy, Aging, and Cancer

  • Ram Hari Pokhrel;Suman Acharya;Sunil Mishra;Ye Gu;Umar Manzoor;Jeon-Kyung Kim;Youngjun Park;Jae-Hoon Chang
    • Biomolecules & Therapeutics
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    • 제32권2호
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    • pp.171-182
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    • 2024
  • All cells are equipped with intricate signaling networks to meet the energy demands and respond to the nutrient availability in the body. AMP-activated protein kinase (AMPK) is among the most potent regulators of cellular energy balance. Under ATP -deprived conditions, AMPK phosphorylates substrates and affects various biological processes, such as lipid/glucose metabolism and protein synthesis. These actions further affect the cell growth, death, and functions, altering the cellular outcomes in energy-restricted environments. AMPK plays vital roles in maintaining good health. AMPK dysfunction is observed in various chronic diseases, making it a promising target for preventing and alleviating such diseases. Herein, we highlight the different AMPK functions, especially in allergy, aging, and cancer, to facilitate the development of new therapeutic approaches in the future.

연령증가에 따른 정상 한국인 대퇴골의 재형성에 관한 연구 (A Study on the Age-related Remodeling of Femur in Normal Korean Adult)

  • 강승백;배태수;최재봉;최귀원
    • 대한의용생체공학회:학술대회논문집
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    • 대한의용생체공학회 1997년도 추계학술대회
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    • pp.489-492
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    • 1997
  • The general pattern of adaptation in the appendicular skeleton with aging is that the subperiosteal apposition of bone occur along with endosteal absorption. This remodeling of diaphysis to a cylinder of larger diameter is hypothesized to serve a mechanical compensatory unction by increasing the moment of inertia as the cortex thins with aging. These findings is only true of the diaphysis of long bone. Measuring the area and inertia at each section of femur, the age-related change of proximal emur and diaphysis is observed. After screening by physical and radiological examination, 200 normal Korean adults divided 5 groups in both male and female based on age. Twenty persons were in each group. One femur in each person was analyzed using CT images. femur scanned with 60 to 80 slices and this images were digitized. Then 2-D images were reconstructed into 3-D images. Using the nonlinear method, normalization and interpolation technique, 7 locations of interest (trochanteric area: 1, 2 subtrochanteric area: 3, 4, isthmic area: 5, 6, 7) were determined. On the each cross section at each location, the area (total, cortical and medullary) and 5 inertia of moment were measured. The results were analyzed statistically. With aging, significant area change occurred mainly in diaphysis and female. In trochanteric area, no significant change was noted. With aging, total and medullary area were increased, but cortical area was not changed. In diaphysis, lateral bendingresistanceincreasedsignificantly. No inertia change was noted in trochanteric area. Anteroposterior bending resistance was constant with aging. In more than age 60, total area and medullary area were larger than that of others. Lateral bending resistance was higher especially in diaphysis. In diaphysis, with aging, the decreased properties is compensated with the increased lateral bending resistance by geometric remodeling. In trochanteric area, no compensation occur. With aging, especially in more than age 60, the higher rate of trochanteric fracture is expected.

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Modulation of senoinflammation by calorie restriction based on biochemical and Omics big data analysis

  • Bang, EunJin;Lee, Bonggi;Noh, Sang-Gyun;Kim, Dae Hyun;Jung, Hee Jin;Ha, Sugyeong;Yu, Byung Pal;Chung, Hae Young
    • BMB Reports
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    • 제52권1호
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    • pp.56-63
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    • 2019
  • Aging is a complex and progressive process characterized by physiological and functional decline with time that increases susceptibility to diseases. Aged-related functional change is accompanied by a low-grade, unresolved chronic inflammation as a major underlying mechanism. In order to explain aging in the context of chronic inflammation, a new integrative concept on age-related chronic inflammation is necessary that encompasses much broader and wider characteristics of cells, tissues, organs, systems, and interactions between immune and non-immune cells, metabolic and non-metabolic organs. We have previously proposed a novel concept of senescent (seno)-inflammation and provided its frameworks. This review summarizes senoinflammation concept and additionally elaborates modulation of senoinflammation by calorie restriction (CR). Based on aging and CR studies and systems-biological analysis of Omics big data, we observed that senescence associated secretory phenotype (SASP) primarily composed of cytokines and chemokines was notably upregulated during aging whereas CR suppressed them. This result further strengthens the novel concept of senoinflammation in aging process. Collectively, such evidence of senoinflammation and modulatory role of CR provide insights into aging mechanism and potential interventions, thereby promoting healthy longevity.

황색종 잎담배의 발효숙성 촉진에 관한 종합적 연구 (A Comprehensive Study on the Forced Aging of Flue-cured Tobacco-Leaves)

  • 배효원
    • Applied Biological Chemistry
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    • 제13권1호
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    • pp.1-27
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    • 1970
  • 황색종 잎담배의 인공발효숙성은 효모처리에 의한 인공발효, 단순한 인공숙성, 2년 저장숙성 및 미처리구로 나누어 미생물학, 물리학, 화학, 생화학적으로 그 변화과정을 종합적으로 연구하여 다음과 같은 결과를 얻었다. 1. 한국산 잎담배 Y.S.A의 발호숙성을 촉진시키는 최적 조건으로서는 및 담배를 온도 $40^{\circ}C$평형함수율 18%, 관계습도 74%에 놓아두는 것이 잎담배의 모든 품질적 특성에 가장 양호한 결과를 주는 것임을 알게되었다. 2. 잎담배를 $40^{\circ}C$의 숙성온도 및 74%의 상대습도에서 효모를 첨가하여 약 20일 내외에서 숙성을 촉진시켜 숙성시키면 잎담배의 여러가지 물리적 및 화학적 특성이 1년 및 2년 저장숙성시킨 것과 비슷하게 됨을 알게 되었음으로 그 실용적 효과를 입증할 수 있었다. 3. 온도 $40^{\circ}C$와 평형함수율 18%의 숙성조건하에서 잎담배에 효모를 첨가하여 또는 단순히 조기숙성시켰을 때 잎담배는 그 숙성에 필요한 물리화학적 특성은 약 20일만에 얻을 수 있다는 것을 알게되었다. 4, 본 실험에서 잎담배의 발효숙성기간중 미생물의 동태는 효모와 세균은 15일까지 증가하였다가 감소하였고 곰팡이류는 끝까지 계속 증가하였다. 5, 효모구 및 속성구 잎담배의 이화학성은 발효숙성이 $15{\sim}20$일 진행함에 따라 pH는 저하하였고 팽승성과 연소성이 양호하여 졌고 자극순도, 명도주재파장등 색택에 관련되는 물리성이 2년저장 잎담배와 비등하여졌다. 또 총환원성물질, 전당, 전환원당, 알카로이드량은 감소하였고 유기산, ether 추출물은 증가, 전질소 및 단백질량, 조섬유, 회분등은 별 변화가 없었다. 6. 효모 및 속성구의 잎담배는 발효숙성이 15-30일 진행되는 동안 그 개개의 화학성분이 다음과 같이 변화하였다. 즉, 시일이 경과함에 따라 당 류-sucrose, rhamnose, glucose 색소류-chlorophyll, carotenes, xanthophyll, violaxanthine polyphenols-rutin, chlorogenic 및 caffeic acid 유기산-iso?tutylic, crotonic, capronic, galacturonic,tartaric, succinic, citric acid 알카로이드-nicotine,nornicotine등 화합물은 감소하였다. 한편 시일이 경과함에 따라 당 류-fructose, maltose, raffnose 아미노산-proline, cystine, 유 기 산-formic, acetic, propionic, malic, oxalic,malonic, α-ketoglutaric, fumaric, glutaric acid 등 화합물은 증가하였다. 7. 잎담배 발효숙성중 생화학적 특성의 변화는 다음과 같았다. 즉, 30일간의 발효숙성기간중 산소흡수량은 점차 감소하였으며, ${\alpha}-amylase$ ${\beta}-amylase$}활성도는 점차 약하여 졌으나 catalase, invertase는 그 활성도가 일단 숙성중기에 높아졌다가 낮아졌다.

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