The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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PCR Analysis for the Discrimination of Leonuri Herba Medicine on the Basis of Chloroplast DNA Sequence Comparison in Six Lamiaceae Species

꿀풀과 6개종의 Chloroplast 부위 유전자를 이용한 익모초(益母草) 감별 PCR 분석

  • Lee, Jae-Woong (Aging Research Center, Korea Institute of Oriental Medicine) ;
  • Kim, Young-Hwa (Aging Research Center, Korea Institute of Oriental Medicine) ;
  • Choi, Go-Ya (Aging Research Center, Korea Institute of Oriental Medicine) ;
  • Ko, Byoung-Seob (Aging Research Center, Korea Institute of Oriental Medicine) ;
  • Kim, Young-Sun (Aging Research Center, Korea Institute of Oriental Medicine) ;
  • Chae, Sung-Wook (Aging Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Hye-Won (Aging Research Center, Korea Institute of Oriental Medicine) ;
  • Oh, Seung-Eun (Department of Biological Sciences, Konkuk University) ;
  • Park, Sang-Un (Department of Agriculture, Chungnam National University) ;
  • Lee, Mi-Young (Aging Research Center, Korea Institute of Oriental Medicine)
  • 이재웅 (한국한의학연구원 노화연구센터) ;
  • 김영화 (한국한의학연구원 노화연구센터) ;
  • 최고야 (한국한의학연구원 노화연구센터) ;
  • 고병섭 (한국한의학연구원 노화연구센터) ;
  • 김영선 (한국한의학연구원 노화연구센터) ;
  • 채성욱 (한국한의학연구원 노화연구센터) ;
  • 이혜원 (한국한의학연구원 노화연구센터) ;
  • 오승은 (건국대학교 이과대학 생명과학과) ;
  • 박상언 (충남대학교 농업생명과학대학) ;
  • 이미영 (한국한의학연구원 노화연구센터)
  • Received : 2011.04.26
  • Accepted : 2011.09.17
  • Published : 2011.09.30
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Abstract

Objectives : The application of polymerase chain reaction (PCR) for the discrimination of the herbal medicine Leonuri Herba (Leonurus japonicus) was evaluated by the comparison of the DNA sequence with Lamiaceae herbal medicine. Method : Genetic analysis showed that phylogenetic tree and comparing sequences through the DNA analysis of rbcL (ribulose-1, 5-bisphosphatecarboxylase) region and trnL-F (tRNA-Leu, trnL-trnF intergeni cspacer, and tRNA-Phe) region of chloroplast DNA from six Lamiaceae sold in market. And we developed IMCF and IMCR primers in order to distinction Leonuri Herba in six Lamiaceae using rbcL and trnL-F sequences. Results : Genetic analysis showed that six Lamiaceae showed individual group on phylogenetic tree. PCR amplification product of Leonuri Herba and another five Lamiaceae were developed for amplification of a 281 bp sequence and the specific PCR amplification of a 460 bp sequence that was exclusive to Leonuri Herba was designed using IMCF and IMCR primers. Conclusion : PCR analysis based on the chloroplast DNA sequences allows the discrimination of Leonuri Herba-based medicine.

Keywords

References

  1. Herbology Editorial Committee of Korean Medicine schools. Herbology [Boncho-hak]. Seoul:Young-Lim Press. 2004:463-4.
  2. Korea Food & Drug Administration. The Korean Pharmacopoeia. Ninth Edition. 2007:966.
  3. State Pharmacopoeia Committee of the People's Republic of China. Pharmacopoeia of the People's Republic of China 2010 Edition. Beijing:People's Medical Publishing House. 2010:272.
  4. Ministry of Health, Labour and Welfare of the Japan. The 16th Edition of the Japanese Pharmacopoeia. 2011:1591.
  5. Wu ZY, Raven PH, eds. Flora of China. Vol. 17(Verbenaceae through Solanaceae). Beijing: Science Press, and St. Louis:Missouri Botanical Garden Press. 1994:163.
  6. Wu CY, Li HW, eds. Flora Republicae Popularis Sinicae. Vol. 65(2). Beijing:Science Press. 1977:508-12.
  7. Song YE, Ku CS, Mun SP, Ryu JS, Kim DH, Choi JS, Choi YG. Volatile aroma compounds and their characteristics of Labiatae by Solid-Phase Microextraction (SPME). Korean J. Medicinal Crop Sci. 2002;10(2):120-5.
  8. Lee JC, Choi YH, KIM YH. Essential oils in aerial parts of Agastache rugosa O. Kuntze. Korean J. Medicinal Crop Sci. 1994;2(2):168-73.
  9. Lee BK, Bang JK, Kim JK, Park CB, Kim KS, Kim NH, Song JS, Lee BH. Chemotaxonomy based on essential oil composition in Elsholtzia ciliata and Agastache rugosa by SDE and headspace. Treat. of Crop Res. 2000;1:425-30.
  10. Lin JJ, Kuo J, Ma J, Saunders JA, Beard HS, MacDonald MH, Kenworthy W, Ude GN, Metthews BF. Identification of molecular markers in soybean comparing RFLP, RAPD and AFLP DNA mapping techniques. Plant Molecular Biology Reporter. 1996;14(2):156-69. https://doi.org/10.1007/BF02684905
  11. Lane DJ, Pace B, Olsen GJ, Stahl DA, Sogin ML, Pace NR. Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc. Natl. Acad. Sci. USA. 1985;82(20):955-9. https://doi.org/10.1073/pnas.82.4.955
  12. Lee W, Park JH. Intraspecific sequence variation of trnL/F intergenic region (cpDNA) in Sedum takesimense Nakai (Crassulaceae) and aspects of geographic distribution. Korean J. Pl. Taxon. 2010;40(3):157-62.
  13. Kim MK, Baigalmaa J, Sun H, Noh JH, Kim SY, Yang DC. Phylogenetic analysis of Ji-Mo (Anemarrhena asphodeloides) on the basis of chloroplast DNA sequences. Korean. J. Medicinal Crop Sci. 2008;16(1):20-6.
  14. Oh SH, Potter D. Description and Phylogenetic positoin of a new angiosperm family, Guamatelaceae, inferred from chloroplast rbcL, atpB and matK sequences. Systematic Botany. 2006;31(4):730-8. https://doi.org/10.1600/036364406779695889
  15. Lee MY, Doh EJ, Kim ES, Kim YW, Ko BS, Oh SE. Application of the multiplex PCR method for discrimination of Artemisia iwayomogi from other Artemisia herbs. Biol. Pharm. Bull. 2008;31(4):685-90. https://doi.org/10.1248/bpb.31.685
  16. Lee JW, Kim YH, Ko BS, Ryuk JA, Oh SE, Park SU, Lee MY. ITS-PCR analysis for the discrimination of Moutan Cortex. Korean. J. Medicinal Crop Sci. 2010;18(1):40-5.
  17. Yu Q, Shen XX, Shen YF, Chen JH, Shi CG, Wang ZA. AFLP analysis of genetic diversity of Leonurus japonicus germplasm resources. Chinese Traditional and Herbal Drugs. 2009;8:1296-9
  18. Wen CX, Wu ZM, Tian W, Liu M, Zhou QM, Xie XL. AFLP analysis of genetic diversity of salvia miltiorrhiza Bge. Acta Agriculturae Boreali-Sinica. 2007;S2:122-5
  19. Shasany AK, Darokar MP, Dhawan S, Gupta AK, Gupta S, Shukla AK, Patra NK, Khanuja SPS. Use of RAPD and AFLP markers to identify inter-and intraspecific hybrids of Mentha. J. Hered. 2005;96(5):542-9. https://doi.org/10.1093/jhered/esi091
  20. Gobert V, Moja S, Colson M, Taberlet P. Hybridization in the section Mentha (Lamiaceae) inferred from AFLP markers. Am. J. Bot. 2002;89(12):2017-23. https://doi.org/10.3732/ajb.89.12.2017
  21. Baigalmaa J, Kim MK, Noh JH, Sun H, Yang DC. Phylogenetic analysis of Schizonepeta Spike on the basis of DNA sequences. Korean J. of Medicinal Crop. Sci. 2009;17(1):46-53
  22. An SM, Ryuk JA, Kim YH, Chae BC, Kim HJ, Kim KH, Kang KK, Ko BS, Lee MY. Genetic analysis of Polygonati Rhizoma and Polygonati Odorati Rhizoma using random amplified microsatellite polymorphism. Korean J. Medicinal Crop Sci. 2006;14(3):125-9.
  23. Percifield RJ, Hawkins JS, McCoy JA, Widrlechner MP, Wendel JF. Genetic Diversity in Hypericum and AFLP Markers for Species-Specific Identification of H. perforatum L. Planta Med 2007;73(15):1614-21. https://doi.org/10.1055/s-2007-993749