• Title/Summary/Keyword: biochemical properties

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Biochemical Characterization of Tannases from Paecilomyces variotii and Aspergillus niger

  • Battestin, Vania;Pinto, Gustavo Adolfo Saavedra;Macedo, Gabriela Alves
    • Food Science and Biotechnology
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    • v.16 no.2
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    • pp.243-248
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    • 2007
  • A biochemical characterization of the tannases from Paecilomyces variotii (produced at Unicamp), Aspergillus niger (purchased from Industrial Kerry Bio-Science) and A. niger cnpat 001 (purchased from Embrapa Agroindustrial Tropical-Brazil) was carried out. P variotii is a new strain obtained from the screening of 500 fungi that were tested for their production of tannase. The biochemical properties of this new tannase from P variotii were determined and compared with those of two other tannase preparations. The tannase produced from P. variotii showed optimum activity at pH 6.5. The functional temperature range of the tannases was from $20-70^{\circ}C$, with optima at $70^{\circ}C$ for P. variotii and at $60^{\circ}C$ for the commercially obtained tannase, whereas A. niger cnpat 001 showed optimum activity at $40^{\circ}C$. The effects of 1 mM preparations of cations and anions, inhibitors, surfactants, and chelators on the tannase activity from P. variotii were also studied.

An Experimental Study on Development of Physical Properties and Durability of Concrete Spread with Inorganic Antibiotics (무기질 항균제 도포에 의한 콘크리트의 경화성상 및 내구성상 향상에 관한 실험적 연구)

  • Kim, Moo-Han;Khil, Bae-Su;Kim, Jae-Hwan;Cho, Bong-Suk;Lee, Eui-Bae
    • Journal of the Korea Institute of Building Construction
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    • v.5 no.3 s.17
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    • pp.75-82
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    • 2005
  • Sewage facilities are positively necessary for environment improvement such as rainwater removal, sewage disposal, preservation of the quality of water and health of the citizens in present-day. Meanwhile, a deterioration of the concrete sewer pipe is increasing rapidly due to the chemical and physical attack and especially biochemical attack that is to say biodeterioration. So, in advanced countries, prediction techniques and corrosion inhibition system for sewer concrete are developed and are being applied. Also, antibiotics were developed already but application of that is low because it is not economical and has no practical use. But, in domestic, countermeasures for the corrosion of sewage concrete are not sufficient and biochemical attack is not reflected in those essentially. In this study, to prevent biochemical corrosion of the sewer concrete, surface of the concrete was spread with liquefied inorganic antibiotics and then its engineering properties were experimentally investigated. As a result, compressive strength of the specimen spread with antibiotics were similar to those of non spread, Both bond strength and abrasion amount of the specimen spread with antibiotics were inferior to non spread. Properties of absorption and air permeability of the specimen spread with antibiotics were superior to non spread. Finally, carbonation depth, chloride ion penetration depth and weight change ration of the specimen spread with antibiotics were smaller than non spread.

Effect of Colloidal Silica on the Photochromic Properties of Hard Coating Films Prepared by Sol-Gel Method (Colloidal Silica가 Sol-Gel 하드 코팅 막의 광 변색 특성에 미치는 영향)

  • Shin, Yong Tak;Lee, Min Ji;Cho, Kyung Sook;Song, Ki Chang
    • Korean Chemical Engineering Research
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    • v.49 no.5
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    • pp.535-540
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    • 2011
  • Organic-inorganic hybrid coating solutions were prepared starting from colloidal silica, lanthanum nitrate and ${\gamma}$-glycidoxypropyl trimethoxysilane by the sol-gel method. Also, spiropyran-doped hard coating solutions were prepared by mixing the spiropyran solution, obtained after dissolving spiropyran dye into tetrahydrofuran solvent, with the organic-inorgnic hybrid coating solutions. The spiropyran-doped hard coating solutions were applied as a thin layer to polycarbonate sheets, and their photochromic properties were investigated. The effect of amount of colloidal silica added into the coating solutions was investigated on the photochromic properties of coating films. Both decoloration rate and pencil hardness of the coating films were increased with increasing the amounts of colloidal silica.

Effect of Solvents on the Photochromic Properties of Spiropyran in Hard Coating Films Prepared by Sol-Gel Method (Spiropyran의 분산용매가 Sol-Gel 하드 코팅 막의 광 변색 특성에 미치는 영향)

  • Kim, Dae Hyun;Shin, Yong Tak;Lee, Ju Yeon;Hong, Wongil;Lee, Bum Suk;Song, Ki Chang
    • Korean Chemical Engineering Research
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    • v.47 no.4
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    • pp.430-435
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    • 2009
  • Spiropyran-doped organic-inorganic hybrid coatings were prepared starting from glycidoxypropyl triethoxysilane and vinyltriethoxysilane by a sol-gel method. They were applied as a thin layer to polycarbonate sheets and their photochromic properties were investigated. The effect of polarity of solvents dissolving the spiropyran was investigated on the photochromic properties. The decoloration rate of the spiropyran decreased with increasing the polarity of solvents dissolving the spiropyran because the open form of the spiropyran was easily stabilized in the polar gel matrix.

Incidence of Bordetella bronchiseptica Infection in Youngnam Swine Herds and the Biochemical Properties of the Organisms Recovered from Pigs with Atrophic Rhinitis (영남지방 돼지의 Bordetella bronchiseptica 감염상황 및 분리균의 생화학적 특성)

  • Jang, Hee-kyung;Kim, Bong Hwan
    • Korean Journal of Veterinary Research
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    • v.28 no.1
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    • pp.75-81
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    • 1988
  • The present study was conducted to investigate the Bordetella bronchiseptica infection in Youngnam swine herds during the period from August 1986 to July 1987 and some properties of the organisms isolated from these Korean swine. B. bronchiseptica was recovered from 25 of 70 (35.7%) glowing pigs of 4 to 10 weeks of age and from 12 of 13 (92.3%) herds. From 115 slaughter pigs, 58(50.4%) pigs were culture positive and the pigs from 13 of 14 (92.9%) herds were found to be infected with B. bronchiseptica. The majority of biochemical and cultural properties of B. bronchiseptica isolated from Korean swine were identical to those of the standard strain employed and some 97.6% of the isolates showed the characters of phase I organism on primary isolation.

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An Exposure Experiment for the Evaluation of Corrosion Properties of Sewage Concrete Covered with Antibiotics (항균제를 도포한 하수시설 콘크리트의 생화학적 부식특성 평가를 위한 폭로시험)

  • Roh, Kyung-Min;Lee, Eui-Bae;Kim, Young-Duk;Lee, Byoung-Ky;Kim, Gyu-Yong;Kim, Moo-Han
    • Proceedings of the Korea Concrete Institute Conference
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    • 2006.05b
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    • pp.73-76
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    • 2006
  • Sewage facilities mainly consisted of concrete structures are being deteriorated seriously by biodeterioration originated from sulfur-oxidizing bacteria. To prevent biochemical corrosion of the sewage concrete, antibiotics which prevent growth of sulfur-oxidizing bacteria were developed. Recently, to evaluate biochemical corrosion properties of concrete, antimicrobial performance and resistance to sulfuric acid were investigated respectively. But, in this study, to evaluate it complexly, concrete specimen covered with antibiotics were exposed in the actual sewage environment and were investigated about corrosion properties after three months. As a result, weight change ratio, nature potential and sulfuric ratio of concrete covered with antibiotics were less than plain concrete.

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Adhesive Properties of Emulsion PSA Polymerized Using Tween Series Nonionic Surfactants (Tween계 비이온 계면활성제를 이용하여 중합한 에멀션점착제의 접착물성)

  • Lim, Tae Kyun;Lee, Myung Cheon
    • Korean Chemical Engineering Research
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    • v.52 no.3
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    • pp.289-293
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    • 2014
  • In making emulsion type pressure sensitive adhesive (PSA), environmentally friendly Tween series nonionic surfactants were used to find out the possibility of substituting the traditional nonyl-phenyl nonionic surfactant (NP-40) by comparing their adhesive properties. Results exhibited that the PSA used Tween series nonionic surfactants showed much better adhesive properties in peel strength and holding power than that used NP-40.

Properties of Enterococcus faecium isolated from the intestineof slaughting pigs (도축돈 장관에서 분리한 Enterococcus faeciurnab 특성)

  • Kim, In-doc;Ahn, Mi-hyun;Seok, Ho-bong
    • Korean Journal of Veterinary Research
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    • v.44 no.4
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    • pp.581-586
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    • 2004
  • The growth characteristics and the properties of the Enterococcus faecium (E. faecium) isolated from intestine of slaughting pig were examined in order to confirm whether it will be used practically as probiotics or not. E. faecium was identified by morphological and biochemical tests including of final confirming by API strep kit. Among 264 samples, 44 isolates (16.7%) of E. faecium were observed as an exellent growth as a range of $1{\times}10^7CFU/ml{\sim}3.3{\times}10^9CFU/ml$ in viable bacterial count on MRI medium. All isolates were shown non-haemolysis on the blood agar exception of 28 isolates shown ${\alpha}$-haemolysis and were identified as E. faecium by biochemical test using API strep enzyme kits. Eight strains (18.2%) were finally selected from which they were excellent sensitivity in showing of the acid-tolerance and the bile-tolerance in compare with reference strain.

Prevalence and factors associated with Blackleg outbreak in north western Gyeongbuk province (경북 문경지방에서 발생한 기종저의 역학적 특성)

  • 장영술;김규태;김영환;김순태;도재철;김대원;김봉환
    • Korean Journal of Veterinary Service
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    • v.25 no.1
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    • pp.75-86
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    • 2002
  • Outbreaks from September 1999 to July 2000 of blackleg in 4 milking and fatting cattle farms in the vicinity of Munkyong(northwestern part of Gyeongbuk province) were investigated biochemical characteristics of isolates and were surveyed epidemiological works during the period of 2 months from August to September 2000. Clostridium chauvoei, the etiological agent of blackleg is a serious pathogen of cattle and sheep causing fever, depression and lameness with high mortality in growing phase of 6 to 18 months old mainly beef cattle. Biochemical and cultural properties of 4 isolated Cl chauvoei were identical to those of reported results and isolates were susceptible to ampicillin, bacitracin, chlorarnphenicol, cephalothin, ciprofloxacin, erythromycin, norfloxacin, penicillin, and tetracycline, while they were resistant to amikacin, colistin, gentamicin, kanamycin, neomycin, streptomycin and trimethoprim-sulfamethoxazole. In the results of epidemiological survey, outbreak farms that concentrated region of milking or fatting cattle in each sites were so close contact within a radius of about 1km that they are separated only by wire fence or shrub harrier and flies, rodents, wild cats and birds could have the chance to move around between those farms. The origin of the outbreak blackleg were uncertain, but the transmission between farms were mainly through the movement of farm personnel, vehicles which were struck the feces or manures from farm and other mechanical factors such as flies, rodents and birds furthermore occurred 4 farms were not vaccinated. In conclusion, a periodic disinfection, vaccination and quarantine prevention are ideal methods of damage reduction by blackleg.