Browse > Article

Biochemical Characterization of Tannases from Paecilomyces variotii and Aspergillus niger  

Battestin, Vania (Food Science Department, Faculty of Food Engineering, Campinas State University)
Pinto, Gustavo Adolfo Saavedra (Embrapa Agroindustrial Tropical)
Macedo, Gabriela Alves (Embrapa Agroindustrial Tropical)
Publication Information
Food Science and Biotechnology / v.16, no.2, 2007 , pp. 243-248 More about this Journal
Abstract
A biochemical characterization of the tannases from Paecilomyces variotii (produced at Unicamp), Aspergillus niger (purchased from Industrial Kerry Bio-Science) and A. niger cnpat 001 (purchased from Embrapa Agroindustrial Tropical-Brazil) was carried out. P variotii is a new strain obtained from the screening of 500 fungi that were tested for their production of tannase. The biochemical properties of this new tannase from P variotii were determined and compared with those of two other tannase preparations. The tannase produced from P. variotii showed optimum activity at pH 6.5. The functional temperature range of the tannases was from $20-70^{\circ}C$, with optima at $70^{\circ}C$ for P. variotii and at $60^{\circ}C$ for the commercially obtained tannase, whereas A. niger cnpat 001 showed optimum activity at $40^{\circ}C$. The effects of 1 mM preparations of cations and anions, inhibitors, surfactants, and chelators on the tannase activity from P. variotii were also studied.
Keywords
Biochemical characterization; tannase; Paecilomyces variotii; residue; fermentation;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 3  (Related Records In Web of Science)
Times Cited By SCOPUS : 3
연도 인용수 순위
1 Saxena S, Saxena RK. Statistical optimization of tannase production from Penicillium variable using fruits (chebulic myrobalan) of Terminalia chebula. Biotechnol. Appl. Bio. 39: 99-106 (2004)   DOI   ScienceOn
2 Batra A, Saxena RK. Potential tannase producers from the genera Aspergillus and Penicillium. Process Biochem. 40: 1553-1557 (2005)   DOI   ScienceOn
3 Lekha PK, Lonsane BK. Comparative titres, location and properties of tannin acyl hydrolase produced by Aspergillus niger PKL 104 in solid-state, liquid surface, and submerged fermentations. Process Biochem. 29: 497-503 (1994)   DOI   ScienceOn
4 Mondal KC, Banerjee D, Jana M, Pati BR. Colorimetric assay method for determination of the tannin acyl hydrolase activity. Anal. Biochem. 295: 168-171 (2001)   DOI   ScienceOn
5 Barthomeuf C, Regerat F, Pouratt H. Production, purification, and characterization of tannase from Aspergillus niger LCF 8. J. Ferment. Technol. 77: 320-323 (1994)
6 Iibuchi S, Minoda Y, Yamada S. Studies on tannin acyl hydrolase of microorganisms. Part III. Purification of the enzyme and some properties of it. Agr. Biol. Chem. Tokyo 32: 803-809 (1968)   DOI
7 Yamada H, Adachi O, Watanbe M, Sato N. Studies on fungal tannase. Fomation, purification, and catalytic properties of tannase of Aspergillus flavus. Agr. Biol. Chem. Tokyo 32: 1070-1078 (1968)   DOI
8 Bradoo S, Gupta R, Saxena RK.. Parametric optimization and biochemical regulation of extracellular tannase from Aspergillus japonicus. Process Biochem. 32: 135-139 (1997)   DOI   ScienceOn
9 Sigma DS, Mooser G. Chemical studies of enzyme active sites. Annu, Rev. Biochem. 44: 889-931 (1975)   DOI   ScienceOn
10 Sakai T, Sakamoto T. Purification and some properties of a protopectin-solubilizing enzyme that has potent activity on sugar beet protopectin. Agr. Biol. Chem. Tokyo 54: 879-809 (1990)   DOI
11 Njoroge RN, Li D, Park JT, Cha H, Kim MS, Kim JW. Characterization and application of a novel thermostable glucoamylase cloned from a hyperthermophilic Archaeon Sulfolobus. Food Sci. Biotechnol. 14: 860-865 (2005)
12 Lopes MFS, Leitao AL, Regalia M, Marques JJF, Carrondo MJT, Crespo MTB. Characterization of a highly thermostable extracellular lipase from Lactobacillus plantarum. Int. J. Food Microbiol. 76: 107-115 (2002)   DOI   ScienceOn
13 Van de Lagemaat J, Pyle DL. Modelling the uptake and growth kinetics of Penicillium glabrum in a tannic acid-containing solidstate fermentation for tannase production. Process Biochem. 40: 1773-1782 (2005)   DOI   ScienceOn
14 Belmares R, Contreras-Esquival JC, Rodriguez-Herrera R, Coronel AR, Aguilar CN. Microbial production of tannase: an enzyme with potential use in food industry. Lebensm.-Wiss. Technol. 37: 857-864 (2004)   DOI   ScienceOn
15 Jinwal UK, Roy U, Chowdhury AR, Bhaduri AP, Roy PK. Purification and characterization of an alkaline lipase from a newly isolated Pseudomonas mendocina PK -12CS and chemoselective hydrolysis of fatty acid ester. Bioorg. Med. Chem. 11: 1041-1046 (2003)   DOI   ScienceOn
16 Kim TU, Gu BG, Jeong JY, Byun SM, Shin YC. Purification and characterization ofa maltotetraose-forming alkaline a-amylase from an alkalophilic Bacillus strain GM8901. Appl. Environ. Microb. 61: 3105-3112 (1995)
17 Reese ET, Maguire A. Surfactants as stimulants of enzymes production by microorganisms. Appl. Environ. Microb. 17: 242-245 (1969)
18 Sharma S, Bhat TK, Dawra RK.. Isolation, purification, and properties of tannase from Aspergillus niger van Thieghem. World J. Microb. Biot. 15: 673-677 (1999)   DOI   ScienceOn
19 Whitaker JR. Principles of enzymology for food sciences. pp. 255-282. In: Enzyme Inhibitors. Oxford University Press, New York, NY, USA (1972)
20 Choi JM, Han J, Yoon BS, Chng JH, Shin DB, Lee SK, Hwang JK, Ryang R. Antioxidant properties of tannic acid its inhibitors effects on paraquat-induced oxidative stress in mice. Food Sci. Biotechnol. 15: 728-734 (2006)   과학기술학회마을
21 Aguilar CN, Gutierrez-Sanchez G. Review: Sources, properties, applications and potential uses of tannin acyl hydrolase. Food Sci. Technol. Int. 7: 373-382 (2001)   DOI
22 Vaquero I, Marcobal A, Munos R. Tannase activity by lactic acid bacteria isolated from grape must and wine. Intern. J. Food Microbiol. 96: 199-204 (2004)   DOI   ScienceOn
23 Aoki K, Shinke H, Nishira H. Purification and some properties of yeast tannase. Agr. Biol. Chem. Tokyo 40: 79-85 (1976)   DOI
24 Koyama H. Purification and characterization of 5-Oxo-L-prolinase (L-pyroglutamate hydrolase) from Alcaligenes sp. F-137. Agr. Biol. Chem. Tokyo 52: 735-741 (1988)   DOI
25 Zhong X, Peng L, Zheng S, Sun Zhizhi, Ren Y, Dong M, Xu A. Secretion, purification, and characterization of a recombinant Aspergillus oryzae tannase in Pichia pastoris. Protein Expres. Purif. 36: 165-169 (2004)   DOI   ScienceOn
26 Vongsuvanlert V, Tani Y. Characterization of D-sorbitol dehydrogenase involved in D-sorbitol production of a methonol yeast, Candida boidinii (Kloeckera sp.) no. 2201. Agr. Biol. Chem. Tokyo 52: 419-426 (1988)   DOI
27 Saxena RK., Davidson WS, Sheoran A, Giri B. Purification and characterization of an alkalibe thermostable lipase from Aspergillus carneus. Process Biochem. 39: 239-247 (2003)   DOI   ScienceOn
28 Iizurni T, Nakamura K, Fukase T. Purification and characterization of a thermostable lipase from newly isolated Pseudomonas sp. KWI-56. Agr. Biol. Chem. Tokyo 54: 1253-1258 (1990)   DOI
29 Kar B, Banerjee R, Bhattacharyya BM. Effect of additives on the behavioural properties of tannin acyl hydrolase. Process Biochem. 38: 1285-1293 (2003)   DOI   ScienceOn
30 Rajkumar S, Nandy SC. Isolation, purification, and some properties of Penicilliun chrysogenum tannase. Appl. Envirom. Microb. 46: 525-527 (1983)
31 Battestin V, Macedo G, Pastore P. Otimizing the fermentation broth for tannase production by a new isolated strain Paecilomyces variotii. abstract no. 118:S49. In: Abstracts: 12th European Congress in Biotechnology. J. Technol. Copenhagen, Denmark (2005)