• Title/Summary/Keyword: biochemical properties

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Purification and Biochemical Properties of Storage Protein SP-2 in Tobacco Budworm (Helicoverpa assulta Guenee) (담배나방 저장단백질 SP-2의 정제 및 생화학적 특성)

  • 정성은;채순용;김선봉;이형철
    • Journal of the Korean Society of Tobacco Science
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    • v.18 no.1
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    • pp.39-48
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    • 1996
  • A storage protein(SP-2) was confirmed in haemolymph during larval-pupal-adult development of tobacco burdworm(Helicoverpa assulta Guenee), and its biochemical characteristics were investigated. The titer of SP-2 showed a peak at mature larva, decreased gradually through the late pupal stage, and became undetectable at adult period. As the results from electrophoretic mnysis, SP-2 was confirmed to be glycolipoprotein(M.W. 332kDa) relatively stable to heat( $\leq$ 68$^{\circ}C$ ). This storage protein was determined to be a tetramer composed of a single subunit with MW of 83kDa, and the isoelectric point was 5.7. The amino acid composition of the SP-2 was characterized. It has relatively high content of methionine and histidine, whereas the contents of tyrosine and phenylalanine were relatively low.

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Study on urea precursor effect on the electroactivities of nitrogen-doped graphene nanosheets electrodes for lithium cells

  • Kim, Ki-Yong;Jung, Yongju;Kim, Seok
    • Carbon letters
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    • v.19
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    • pp.40-46
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    • 2016
  • Nitrogen-atom doped graphene oxide was considered to prevent the dissolution of polysulfide and to guarantee the enhanced redox reaction of sulfur for good cycle performance of lithium sulfur cells. In this study, we used urea as a nitrogen source due to its low cost and easy preparation. To find the optimum urea content, we tested three different ratios of urea to graphene oxide. The morphology of the composites was examined by field emission scanning electron microscope. Functional groups and bonding characterization were measured by X-ray photoelectron spectroscopy. Electrochemical properties were characterized by cyclic voltammetry in an organic electrolyte solution. Compared with thermally reduced graphene/sulfur (S) composite, nitrogen-doped graphene/S composites showed higher electroactivity and more stable capacity retention.

Electrochemical characterization of activated carbon-sulfur composite electrode in organic electrolyte solution

  • Kim, Dongyoung;Park, Soo-Jin;Jung, Yongju;Kim, Seok
    • Carbon letters
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    • v.14 no.2
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    • pp.126-130
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    • 2013
  • In this study, we present a more electrochemically enhanced electrode using activated carbon (AC)-sulfur (S) composite materials, which have high current density. The morphological and micro-structure properties were investigated by transmission electron microscopy. Quantity of sulfur was measured by thermogravimetric analysis analysis. The electrochemical behaviors were investigated by cyclic voltammetry. As a trapping carbon structure, AC could provide a porous structure for containing sulfur. We were able to confirm that the AC-S composite electrode had superior electrochemical activity.

Capacitance behaviors of Polyaniline/Graphene Nanosheet Composites Prepared by Aniline Chemical Polymerization

  • Kim, Jieun;Park, Soo-Jin;Kim, Seok
    • Carbon letters
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    • v.14 no.1
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    • pp.51-54
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    • 2013
  • In this study, polyaniline (PANI)/graphene nanosheet (GNS) composites were synthesized through chemical oxidation polymerization by changing the weight ratio of aniline monomers. To examine the morphological structure of the composites, scanning electron microscopy and transmission electron microscopy (TEM) were conducted. TEM results revealed that fibril-like PANI with a diameter of 50 nm was homogeneously coated on the surface of the GNS. The electrochemical properties of the composites were studied by cyclic voltammetry in 1 M $H_2SO_4$ electrolyte. Among the prepared samples, the PANI/GNS (having 40 wt% aniline content) showed the highest specific capacitance, 528 $Fg^{-1}$, at 10 $mVs^{-1}$. The improved performance was attributed to the GNS, which provides a large number of active sites and good electrical conductivity. The resulting composites are promising electrode materials for high capacitative supercapacitors.

Preparation and characterization of Melamine-Formaldehyde Resin Microcapsules Containing Fragrant Oil

  • Hwang, Jun-Seok;Kim, Jin-Nam;Wee, Young-Jung;Yun, Jong-Sun;Jang, Hong-Gi;Kim, Sun-Ho;Ryu, Hwa-Won
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.4
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    • pp.332-336
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    • 2006
  • In this study, melamine-formaldehyde microcapsules were prepared via in situ polymerization using peppermint oil as a core material, melamine-formaldehyde as the wall material, Tween 20 as the emulsifier, and poly (vinyl alcohol) as a protective colloid. The melamine-formaldehyde microcapsules prepared in this study were then evaluated with regard to their structures, thermal properties, particle size distributions, morphologies, and release behaviors.

Preparation and Characterization of Hydrogels containing Silicone or Fluorine

  • Kim, Eui Seok;Shim, Sang-Yeon
    • Journal of the Korean Applied Science and Technology
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    • v.34 no.3
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    • pp.650-656
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    • 2017
  • The water-swollen hydrogels containing silicone or fluorine were prepared by copolymerization of 2-hydroxy ethyl methacylate (HEMA) with 3-(trimethoxysilyl)propyl methacrylate(SM) or 2,2,2-trifluoroethyl acrylate(FA). When the content of SM or FA increased in copolymers, there was tendency of water absorbance to decrease, whereas contact angles to increase. The hydrogels containing FA showed 2 ~ 4% higher water content and 4 ~ 5% lower contact angles compared to that of SM. Tensile strengths decreased as the content of SM increased. However, FA exhibited strength of $2.2Mpa/cm^2$ which is similar to $2.3Mpa/cm^2$ of B. FA, which implies comparatively low adherence, hence, showed better protein resistance properties than SM-based hydrogel. The photo-polymerization was also applied instead of thermal polymerization to enhance the energy efficiency. As a result, the reaction yield reached over 95% within 1 minute.

Study on electrochemical performances of sulfur-containing graphene nanosheets electrodes for lithium-sulfur cells

  • Son, Ki-Soo;Kim, Seok
    • Carbon letters
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    • v.15 no.2
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    • pp.113-116
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    • 2014
  • Due to their morphology, electrochemical stability, and function as a conducting carbon matrix, graphene nanosheets (GNS) have been studied for their potential roles in improving the performance of sulfur cathodes. In this study, a GNS/sulfur (GNS/S) composite was prepared using the infiltration method with organic solvent. The structure, morphology and crystallinity of the composites were examined using scanning electron microscopy, transmission electron microscopy, and X-ray diffraction. The electrochemical properties were also characterized using cyclic voltammetry (CV). The CV data revealed that the GNS/S composites exhibited enhanced specific-current density and ~10% higher capacity, in comparison with the S-containing, activated-carbon samples. The composite electrode also showed better cycling performance for multiple charge/discharge cycles. The improvement in the capacity and cycling stability of the GNS/S composite electrode is probably related to the fact that the graphene in the composite improves conductivity and that the graphene is well dispersed in the composites.

Fungal and mushroom hydrophobins: A review

  • Wu, Yuanzheng;Li, Jishun;Yang, Hetong;Shin, Hyun-Jae
    • Journal of Mushroom
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    • v.15 no.1
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    • pp.1-7
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    • 2017
  • Hydrophobins are surface active proteins that are produced by filamentous fungi including mushrooms. Their ability to self-assemble into an amphipathic membrane at any hydrophilic-hydrophobic interface is most intriguing. These small secreted proteins comprise of eight conserved cysteine residues which form four disulfide bridges and an extraordinary hydrophobic patch. Hydrophobins play critical roles in fungal (and/or mushrooms) growth as structural components and in the interaction of fungi and mushrooms with the environment. The biophysical and biochemical properties of the isolated proteins are remarkable, such as strong adhesion, high surface activity and the formation of various self-assembled structures. With the increasing demands of hydrophobins from fungi and mushroom sources, production and purification in large scale is under challenge. Various applications, ranging from food industries, cosmetics, nanotechnology, biosensors and electrodes, to biomaterials and pharmaceuticals are emerging and a bright future is foreseen.

Antigenicity Studies of the Aqueous Extract of Fresh Ginseng in Guinea Pigs

  • Lee, Jong-Wha;Rhee, Man-Hee;Park, Jong-Il;Han, Sang-Seop
    • Korean Journal of Pharmacognosy
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    • v.25 no.1
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    • pp.35-40
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    • 1994
  • Aqueous extreact of fresh ginseng (AFG) was examined for the antigenicity in Hartley guinea pigs in comparision with ovalbumin (OVA). When guinea pigs were sensitized with AFG emulsified with complete Freund's adjuvant (CFA), these animals showed negative reactions in active systemic anaphylaxis (ASA), active cutaneous anaphylaxis (ACA) and passive cutaneous anaphylaxis (PCA) tests and passive hemagglutination (PHA) reaction. On the contrary, when guinea pigs were sensitized with OVA emulsified with CFA as positive controls, these animals disclosed positive reactions in ASA, ACA and PCA tests and PHA reaction. From these results, AFG was considered not to possess antigenic properties in guinea pigs. In addition, the dose levels of AFG empolyed in the present experiment were confirmed not to suppress immune reactions.

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Investigating Biochemical Properties of Bacillus aryabhattai DA2 from Diesel-Contaminated Soil

  • Kim, Sang-Jun;Adhikari, Arjun;Lee, Ko-Eun;Joo, Gil-Jae
    • Korean Journal of Environmental Biology
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    • v.36 no.2
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    • pp.199-205
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    • 2018
  • Petroleum energy is the major source of the world energy market, and its massive usage, and the corresponding extreme environmental pollution, imposes a serious threat on the ecological cycles. By screening oil-contaminated soil, we isolated, identified, and characterized a novel strain that represents a considerable diesel-degrading potentiality; the Bacillus aryabhattai DA2 strain is registered in the NCBI with the accession number MG571630, and it possesses an efficient tributyrin-degrading capacity. The optimal condition for diesel degradation by DA2 strain was observed at pH between 7-8 and at the temperature of $30^{\circ}C$. The strain is resistant to salt as well as the antibiotics like ampicillin and streptomycin. These results indicate B. aryabhattai is one of the potential candidates for the remediation of the diesel-contaminated sites.