• 전기학회논문지
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• 제58권12호
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• pp.2474-2479
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• 2009

This paper presents an atmospheric microplasma-jet device for bio~medical application. The microplasma-jet device consists of four components; a thin Ni anode, porous alumina insulator, a stainless steel cathode and an aluminum case. The anode has 8 holes, and hole diameter and depth are $200 {\mu}m$ and $60 {\mu}m$, respectively. The discharge test was performed in atmospheric pressure using nitrogen gas and AC voltage at the optimum gas flow rate of 4 Vmin. The plasma-jet is ejected stably for the input voltage ranging from 5.5 to $9.5 kV_{p-p}$. The plasma becomes dense as the input voltage increases, which was verified by the hydrophilicity change of PMMA surface treated by the plasma. The temperature increasement of the aluminum film exposed to plasma-jet illustrates that the micro plasma-jet device is feasible for bio-medical application.

• BMB Reports
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• 제40권1호
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• pp.7-14
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• 2007

Helicases are ubiquitous enzymes, which utilize the energy liberated during nucleotide triphosphate hydrolysis to separate double-stranded nucleic acids into single strands. These enzymes are very attractive targets for the development of new antibacterial compounds. The PcrA DNA helicase from Staphylococcus aureus is a good candidate for drug discovery. This enzyme is unique in the genome of S. aureus and essential for this bacterium. Furthermore, it has recently been published that it is possible to identify inhibitors of DNA helicases such as PcrA. In this report, we study the properties of recombinant PcrA from S. aureus purified from Escherichia coli to develop ATPase and helicase assays to screen for inhibitors.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2023년도 추계학술발표대회
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• pp.1004-1005
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• 2023

본 연구에서는 PVDF 압전 센서와 IR 체온 센서 등을 활용하여 비접촉, 무(無) 구속 방식으로 요람에 있는 영유아의 바이탈 신호를 상시 측정하여 '영아 돌연사 증후군' 등 위험한 상황에 빠졌을 때 애플리케이션을 통하여 즉각 보호자에게 알리고 대처 방안을 제시하며, 상시 영·유아 모니터링이 불가능한 맞벌이 부모 등에게 자동 상태 경보 서비스를 제공하기 위하여 스마트 요람을 개발하였다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.967-967
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• 2005

• 한국의학물리학회:학술대회논문집
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• 한국의학물리학회 2006년도 제33회 추계학술대회 발표논문집
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• pp.25-25
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• 2006

• 한국정밀공학회지
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• 제23권11호
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• pp.24-33
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• 2006

• Korean Journal of Acupuncture
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• 제29권1호
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• pp.71-81
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• 2012

Objectives : The purpose of this paper was to suggest new diagnostic method that was to supersede the estimation of electrical properties at acupoints. Thus, we developed the multi- frequencies bioelectrical impedance measurement system so as to analyze the state of bio-ions in body fluid as body compositions, not skin impedance at acupoint. Methods : At low frequency, the current does not penetrate the cell membrane and at high frequency, the current passes through both intracellular and extracellular fluid because of the decreas of cell membrane impedance. To confirm the reflection of composition in extracellular fluid or intracellular fluid of segment such as acupoint, the system was developed to detect the acupoint potential between adjacent two points in the area of LU3, LU4 and LU9 using 5,50 and 200KHz. Results : The detected acupoint potential has been decreased according to elevation of frequency. As a result of correlation of left/right identical acupoint, we observed a high correlation of three types of acupoint potential at multi-frequencies. Moreover, we observed the low correlation at 5KHz, and that was a significant factor to be considered as unbalanced relationship of identical acupoints. Conclusions : On the basis of meridian theoretical point of view, we may infer the acupoint's physiological composition using the multi-frequencies bioelectrical impedance measurement system.

• 디지털융복합연구
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• 제11권4호
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• pp.311-320
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• 2013

본 연구는 일 대학 간호과와 비 간호과 학생의 생명의료윤리 의식을 파악하여 대학생들의 올바른 생명윤리 의식 함양에 필요한 윤리교육의 방향과 교육과정 내용을 위한 기초자료를 제공하고자 시도하였다. 생명의료윤리 의식은 4점 만점에 간호과 학생들이 평균 3.04점으로 비 간호과 학생들의 2.88점보다 높았고 이들 간에 유의한 차이가 있었다(t=6.79, p<.001). 두 그룹간에 태아의 생명권, 태아진단, 신생아 생명권, 안락사, 장기이식, 뇌사영역에서 통계적으로 유의한 차이를 나타내었다. 대상자들의 생명의료윤리 의식에 영향을 미치는 주요 요인은 간호과에서는 한 학기 이상 윤리관련 수업 경험 유무, 생명윤리 관련 문제로 고민한 경험 유무, 윤리적 가치관이었고, 비 간호과는 학년으로 나타났다. 결과적으로 대상자들의 낮은 생명의료윤리 의식은 각 학과별로 기본적인 생명윤리 교육부터 고학년으로 올라갈수록 체계적이고 복합적인 윤리적 사고 판단과 의사결정 능력, 가치관 고취를 위한 교육과정을 통해 충족되어야 할 것이다. 추후 생명의료윤리 의식의 하위영역별 집중연구나 질적 연구를 제언한다.

• 자연과학논문집
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• 제14권2호
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• pp.93-102
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• 2004

A yeast strain SL-27 found to produce active intracellular tyrosinase inhibitor was screened from 972 kinds of yeasts. It was identified as Malassezia pachydermatis based on microbiological characteristics. The optimum pH and temperature for the growth of Malassezia pachydermatis SL-27 were pH 7.0 and $37^{\circ}C$, respectively. The optimal culture conditions for the production of tyrosinase inhibitor by Malassezia pachydermatis SL-27 were investigated. The optimal medium cimposition for tyrosinase inhibitor production was determined to be 1.0% casamino acid, 2.0% glucose, 0.1% $KH_2PO_4$, 0.05% $MgSo_{4-}7H20$ and each 0.01 of $CaCl_2$ and NaCl. Optimal initial pH and temperature for the production of tyrosinase inhibitor were pH 5.0 and $30^{\circ}C$, respectively. The maximum tyrosinase inhibitory activity of 84%/mL of cell-free extract was showed after 12 h of cultivation under the optimal culturing conditions.

• Mycobiology
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• 제49권6호
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• pp.582-588
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• 2021

The interaction of mating pheromone and pheromone receptor from the B mating-type locus is the first step in the activation of the mushroom mating signal transduction pathway. The B mating-type locus of Lentinula edodes is composed of Bα and Bβ subloci, each of which contains genes for mating pheromone and pheromone receptor. Allelic variations in both subloci generate multiple B mating-types through which L. edodes maintains genetic diversity. In addition to the B mating-type locus, our genomic sequence analysis revealed the presence of a novel chromosomal locus 43.3 kb away from the B mating-type locus, containing genes for a pair of mating pheromones (PHBN1 and PHBN2) and a pheromone receptor (RCBN). The new locus (Bα-N) was homologous to the Bα sublocus, but unlike the multiallelic Bα sublocus, it was highly conserved across the wild and cultivated strains. The interactions of RcbN with various mating pheromones from the B and Bα-N mating-type loci were investigated using yeast model that replaced endogenous yeast mating pheromone receptor STE2 with RCBN. The yeast mating signal transduction pathway was only activated in the presence of PHBN1 or PHBN2 in the RcbN producing yeast, indicating that RcbN interacts with self-pheromones (PHBN1 and PHBN2), not with pheromones from the B mating-type locus. The biological function of the Bα-N locus was suggested to control the expression of A mating-type genes, as evidenced by the increased expression of two A-genes HD1 and HD2 upon the treatment of synthetic PHBN1 and PHBN2 peptides to the monokaryotic strain of L. edodes.