Mycobiology

The Korean Society of Mycology (한국균학회)
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Discovery and Functional Study of a Novel Genomic Locus Homologous to Bα-Mating-Type Sublocus of Lentinula edodes

  • Lee, Yun Jin (Department of Bio & Medical Big Data (BK4 program) and Research Institute of Life Sciences, Gyeongsang National University) ;
  • Kim, Eunbi (Department of Bio & Medical Big Data (BK4 program) and Research Institute of Life Sciences, Gyeongsang National University) ;
  • Eom, Hyerang (Department of Bio & Medical Big Data (BK4 program) and Research Institute of Life Sciences, Gyeongsang National University) ;
  • Yang, Seong-Hyeok (Department of Bio & Medical Big Data (BK4 program) and Research Institute of Life Sciences, Gyeongsang National University) ;
  • Choi, Yeon Jae (Department of Bio & Medical Big Data (BK4 program) and Research Institute of Life Sciences, Gyeongsang National University) ;
  • Ro, Hyeon-Su (Department of Bio & Medical Big Data (BK4 program) and Research Institute of Life Sciences, Gyeongsang National University)
  • Received : 2021.09.27
  • Accepted : 2021.10.30
  • Published : 2021.12.31
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Abstract

The interaction of mating pheromone and pheromone receptor from the B mating-type locus is the first step in the activation of the mushroom mating signal transduction pathway. The B mating-type locus of Lentinula edodes is composed of Bα and Bβ subloci, each of which contains genes for mating pheromone and pheromone receptor. Allelic variations in both subloci generate multiple B mating-types through which L. edodes maintains genetic diversity. In addition to the B mating-type locus, our genomic sequence analysis revealed the presence of a novel chromosomal locus 43.3 kb away from the B mating-type locus, containing genes for a pair of mating pheromones (PHBN1 and PHBN2) and a pheromone receptor (RCBN). The new locus (Bα-N) was homologous to the Bα sublocus, but unlike the multiallelic Bα sublocus, it was highly conserved across the wild and cultivated strains. The interactions of RcbN with various mating pheromones from the B and Bα-N mating-type loci were investigated using yeast model that replaced endogenous yeast mating pheromone receptor STE2 with RCBN. The yeast mating signal transduction pathway was only activated in the presence of PHBN1 or PHBN2 in the RcbN producing yeast, indicating that RcbN interacts with self-pheromones (PHBN1 and PHBN2), not with pheromones from the B mating-type locus. The biological function of the Bα-N locus was suggested to control the expression of A mating-type genes, as evidenced by the increased expression of two A-genes HD1 and HD2 upon the treatment of synthetic PHBN1 and PHBN2 peptides to the monokaryotic strain of L. edodes.

Keywords

Acknowledgement

This work was financially supported by a grant from the New Breeding Technologies Development Program (Project No. PJ01516502), Rural Development Administration, Republic of Korea.

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