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http://dx.doi.org/10.1080/12298093.2021.2001906

Discovery and Functional Study of a Novel Genomic Locus Homologous to Bα-Mating-Type Sublocus of Lentinula edodes  

Lee, Yun Jin (Department of Bio & Medical Big Data (BK4 program) and Research Institute of Life Sciences, Gyeongsang National University)
Kim, Eunbi (Department of Bio & Medical Big Data (BK4 program) and Research Institute of Life Sciences, Gyeongsang National University)
Eom, Hyerang (Department of Bio & Medical Big Data (BK4 program) and Research Institute of Life Sciences, Gyeongsang National University)
Yang, Seong-Hyeok (Department of Bio & Medical Big Data (BK4 program) and Research Institute of Life Sciences, Gyeongsang National University)
Choi, Yeon Jae (Department of Bio & Medical Big Data (BK4 program) and Research Institute of Life Sciences, Gyeongsang National University)
Ro, Hyeon-Su (Department of Bio & Medical Big Data (BK4 program) and Research Institute of Life Sciences, Gyeongsang National University)
Publication Information
Mycobiology / v.49, no.6, 2021 , pp. 582-588 More about this Journal
Abstract
The interaction of mating pheromone and pheromone receptor from the B mating-type locus is the first step in the activation of the mushroom mating signal transduction pathway. The B mating-type locus of Lentinula edodes is composed of Bα and Bβ subloci, each of which contains genes for mating pheromone and pheromone receptor. Allelic variations in both subloci generate multiple B mating-types through which L. edodes maintains genetic diversity. In addition to the B mating-type locus, our genomic sequence analysis revealed the presence of a novel chromosomal locus 43.3 kb away from the B mating-type locus, containing genes for a pair of mating pheromones (PHBN1 and PHBN2) and a pheromone receptor (RCBN). The new locus (Bα-N) was homologous to the Bα sublocus, but unlike the multiallelic Bα sublocus, it was highly conserved across the wild and cultivated strains. The interactions of RcbN with various mating pheromones from the B and Bα-N mating-type loci were investigated using yeast model that replaced endogenous yeast mating pheromone receptor STE2 with RCBN. The yeast mating signal transduction pathway was only activated in the presence of PHBN1 or PHBN2 in the RcbN producing yeast, indicating that RcbN interacts with self-pheromones (PHBN1 and PHBN2), not with pheromones from the B mating-type locus. The biological function of the Bα-N locus was suggested to control the expression of A mating-type genes, as evidenced by the increased expression of two A-genes HD1 and HD2 upon the treatment of synthetic PHBN1 and PHBN2 peptides to the monokaryotic strain of L. edodes.
Keywords
Mating pheromone; receptor; Lentinula edodes; activation;
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1 Caldwell GA, Naider F, Becker JM. Fungal lipopeptide mating pheromones: a model system for the study of protein prenylation. Microbiol Rev. 1995;59(3):406-422.   DOI
2 Wang W, Lian L, Xu P, et al. Advances in understanding mating type gene organization in the mushroom-forming fungus Flammulina velutipes. G3. 2016;6(11):3635-3645.   DOI
3 Ohm RA, de Jong JF, Lugones LG, et al. Genome sequence of the model mushroom Schizophyllum commune. Nat Biotechnol. 2010;28(9):957-963.   DOI
4 Michaelis S, Barrowman J. Biogenesis of the Saccharomyces cerevisiae pheromone a-factor, from yeast mating to human disease. Microbiol Mol Biol Rev. 2012;76(3):626-651.   DOI
5 Riquelme M, Challen MP, Casselton LA, et al. The origin of multiple B mating specificities in Coprinus cinereus. Genetics. 2005;170(3):1105-1119.   DOI
6 Olesnicky NS, Brown AJ, Honda Y, et al. Self-compatible B mutants in Coprinus with altered pheromone-receptor specificities. Genetics. 2000;156(3):1025-1033.   DOI
7 Halsall JR, Milner MJ, Casselton LA. Three subfamilies of pheromone and receptor genes generate multiple B mating specificities in the mushroom Coprinus cinereus. Genetics. 2000;154(3):1115-1123.   DOI
8 Parag Y, Koltin Y. The structure of the incompatibility factors of Schizophyllum commune: constitution of the three classes of B factors. Mol Gen Genet. 1971;112(1):43-48.   DOI
9 Ha B, Kim S, Kim M, et al. Activation of the mating pheromone response pathway of Lentinula edodes by synthetic pheromones. Mycobiology. 2018;46(4):407-415.   DOI
10 Kim S, Ha B, Kim M, et al. Investigation of mating pheromone-pheromone receptor specificity in Lentinula edodes. Genes. 2020;11(5):506.   DOI
11 Shim D, Park SG, Kim K, et al. Whole genome de novo sequencing and genome annotation of the world popular cultivated edible mushroom, Lentinula edodes. J Biotechnol. 2016;223:24-25.   DOI
12 Michaelis S, Herskowitz I. The a-factor pheromone of Saccharomyces cerevisiae is essential for mating. Mol Cell Biol. 1988;8(3):1309-1318.   DOI
13 Casselton LA, Olesnicky NS. Molecular genetics of mating recognition in basidiomycete fungi. Microbiol Mol Biol Rev. 1998;62(1):55-70.   DOI
14 Wu L, van Peer A, Song W, et al. Cloning of the Lentinula edodes B mating-type locus and identification of the genetic structure controlling B mating. Gene. 2013;531(2):270-278.   DOI
15 Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016;33(7):1870-1874.   DOI
16 Ha B, Moon YJ, Song Y, et al. Molecular analysis of B mating-type diversity in Lentinula edodes. Sci Hortic. 2019;243:55-63.   DOI
17 Kues U. From two to many: multiple mating-types in basidiomycetes. Fungal Biol Rev. 2015;29:126-166.   DOI
18 Ju Y, Kim S, Kim M, et al. Structure analysis of a and B mating-type loci in a representative commercial strain of Pleurotus eryngii. Sci Horti. 2020;274:109686.   DOI
19 Wirth S, Freihorst D, Krause K, et al. What role might non-mating receptors play in Schizophyllum commune? J Fungi. 2021;7(5):399.   DOI
20 Kues U. Life history and developmental processes in the basidiomycete Coprinus cinereus. Microbiol Mol Biol Rev. 2000;64:316-353.   DOI
21 O'Shea SF, Chaure PT, Halsall JR, et al. A large pheromone and receptor gene complex determines multiple B mating type specificities in Coprinus cinereus. Genetics. 1998;148(3):1081-1090.   DOI
22 Raper JR, Krongelb GS, Baxter MG. The number and distribution of incompatibility factors in Schizophyllum commune. Am Nat. 1958;92(865):221-232.   DOI
23 Nakayama N, Miyajima A, Arai K. Nucleotide sequences of ste2 and ste3, cell type-specific sterile genes from Saccharomyces cerevisiae. EMBO J. 1985;4(10):2643-2648.   DOI