• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.29-29
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• 2022

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2013.05a
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• pp.1511-1512
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• 2013

• Journal of International Society for Simulation Surgery
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• v.3 no.2
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• pp.49-59
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• 2016

3D printing is also known as additive manufacturing technique in which has been used in various commercial fields such as engineering, art, education, and medicine. The applications such as fabrication of tissues and organs, implants, drug delivery, creation surgical models using 3D printer in medical field are expanding. Recently, 3D printing has been developing for produce biomimetic 3D structure using biomaterials containing living cells and that is commonly called "3D bio-printing". The 3D bio-printing technologies are usually classified four upon printing methods: Laser-assisted printing, Inkjet, extrusion, and stereolithograpy. In the bio-printing, bio-inks (combined hydrogels and living cells) are as important components as bio-printing technologies. The presence of various types of bioinks, however, in this review, we focused on the bio-inks which enables bioprinting efficacy using hydrogels with living cells.

• Journal of Microbiology and Biotechnology
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• v.27 no.12
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• pp.2156-2164
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• 2017

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is considered as an antitumor agent owing to its ability to induce apoptosis of cancer cells without imparting toxicity toward most normal cells. TRAIL is produced in poor yield because of its insoluble expression in the cytoplasm of E. coli. In this study, we achieved soluble expression of TRAIL by fusing maltose-binding protein (MBP), b'a' domain of protein disulfide isomerase (PDIb'a'), or protein disulfide isomerase at the N-terminus of TRAIL. The TRAIL was purified using subsequent immobilized metal affinity chromatography and amylose-binding chromatography, with the tag removal using tobacco etch virus protease. Approximately 4.5 mg of pure TRAIL was produced from 125 ml flask culture with a purification yield of 71.6%. The endotoxin level of the final product was $0.4EU/{\mu}g$, as measured by the Limulus amebocyte lysate endotoxin assay. The purified TRAIL was validated and shown to cause apoptosis of HeLa cells with an $EC_{50}$ and Hill coefficient of $0.6{{\pm}}0.03nM$ and $2.41{\pm}0.15$, respectively. The high level of apoptosis in HeLa cells following administration of purified TRAIL indicates the significance and novelty of this method for producing high-grade and high-yield TRAIL.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.9
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• pp.4229-4233
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• 2012

With the advances in modern diagnostic expertise for cancer, certain approaches allowing scanning of the complete genome and the proteome are becoming very useful for researchers. These high throughput techniques have already proven power, over traditional detection methods, in differentiating disease sub-types and identifying specific genetic events during progression of cancer. This paper introduces major branches of omics-technology and their applications in the field of cancer. It also addresses current road blocks that need to be overcome and future possibilities of these methods in oncogenic detection.

• Proceedings of the Korean Society of Computer Information Conference
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• 2012.07a
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• pp.409-410
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• 2012

본 논문에서는 스마트케어 서비스를 위해, Android 운영체제를 기반으로 하는 Device에서 ADK를 이용해 혈압계를 제어하는 방법을 제안한다. 이 방법은 소프트웨어와 하드웨어를 결합한 kit인 ADK를 사용하여 Hardware를 잘 모르는 사람들도 Android에서 Hardware의 GPIO에 좀 더 쉽게 접근하고 제어할 수 있어 보다 다양한 기능을 구현할 수 있다. 또한 이 방법으로 얻은 혈압계 데이터를 Database에 저장하여 데이터통신망을 통해 전문의에게 Feedback을 받음으로써 굳이 병원을 방문하지 않더라도 개인의 건강을 보다 편리하게 관리할 수 있어 한 단계 수준 높은 진단을 받을 수 있다.

• Journal of the Korea Convergence Society
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• v.6 no.6
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• pp.255-261
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• 2015

The purpose of this study is to study of correlation of characteristcs of bio-medical ethics and self-esteem for undergraduate students and to compare bio-medical ethics perception between health science and non-health science undergraduate students. We investigated bio-medical ethics and self-esteem of 651 undergraduate students including health science students and non-health science students for two weeks in May, 2015. The results of this study showed significant correlation between self-esteem and bio-medical ethics. The higher self-esteem, the more important bio-medical ethics. But it showed no significant difference between two groups in bio-medical ethics about brain death, euthanasia except biotechnology.

• 한국초전도학회:학술대회논문집
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• 2007.07a
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• pp.52-52
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• 2007

• The Korean Journal of Physiology and Pharmacology
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• v.23 no.5
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• pp.335-344
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• 2019

Obesity causes inflammation and impairs thermogenic functions in brown adipose tissue (BAT). The adipokine lipocalin 2 (LCN2) has been implicated in inflammation and obesity. Herein, we investigated the protective effects of caloric restriction (CR) on LCN2-mediated inflammation and oxidative stress in the BAT of high-fat diet (HFD)-fed mice. Mice were fed a HFD for 20 weeks and then either continued on the HFD or subjected to CR for the next 12 weeks. CR led to the browning of the white fat-like phenotype in HFD-fed mice. Increased expressions of LCN2 and its receptor in the BAT of HFD-fed mice were significantly attenuated by CR. Additionally, HFD+CR-fed mice had fewer neutrophils and macrophages expressing LCN2 and iron-positive cells than HFD-fed mice. Further, oxidative stress and mitochondrial fission induced by a HFD were also significantly attenuated by CR. Our findings indicate that the protective effects of CR on inflammation and oxidative stress in the BAT of obese mice may be associated with regulation of LCN2.

• Perspectives in Nursing Science
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• v.5 no.1
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• pp.45-58
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• 2008

The nursing informatics has been booming in Taiwan since 2003 when we started to use the end user computing strategy to promote it. We used Excel 2003, which was well known and used by our clinical nurses, as well as the embedded VBA to teach them how simple information applications could and should be built to meet their information management needs in order to support their professional responsibility. Many cost-effective projects were successfully done and the importance and potentials of nursing informatics started to be noticed. Our training strategy and materials are introduced in this paper.