• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.7
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• pp.907-913
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• 2012

Piceatannol (trans-3,4,3',5'-tetrahydroxystilbene) is a polyphenol detected in grapes, rhubarb, and sugarcane. Although recent experimental data revealed that this compound is known to exhibit immunosuppressive and antitumorigenic activities in several cell lines, the molecular mechanisms underlying anticancer activity are poorly understood. In the present study, we investigated possible further mechanisms by which piceatannol exerts its anti-proliferative action in cultured human gastric cancer AGS cells. Piceatannol treatment resulted in the inhibition of growth and G1 arrest of the cell cycle in a concentration-dependent manner, as determined by MTT assay and flow cytometry analysis. The induction of G1 arrest by piceatannol was associated with the modulation of cyclin-dependent kinases (Cdks) and cyclins, up-regulation of the expression of Cdk inhibitor p21 (WAF1/CIP1) in both transcriptional and translational levels, and the inhibition of phosphorylation of retinoblastoma proteins and E2F1 expression. In addition, piceatannol treatment caused a progressive decrease in the expression levels of cyclooxygenase (COX)-2 without significant changes in the levels of COX-1, which was correlated with a decrease in prostaglandin $E_2$ synthesis.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.6
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• pp.782-789
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• 2012

This study was designed to evaluate the anti-metabolic syndrome effects of capsule-filled cheonggukjang (CGJ) added with arrowroot (Pueraria thunbergiana) extracts on body weight, adiposity and lipid metabolism in ob/ob mice. Experimental groups were normal control group (NC: basal diet), positive control group (PC: 2% CGJ), CGJ added with arrowroot extracts group (AR: 2% arrowroot in CGJ), and capsule-filled CGJ added with arrowroot extracts group (ARC: 2% arrowroot CGJ capsule). Each group was fed experimental diet for 10 weeks. Final body weight gain and atherogenic index were significantly lower in the ARC than NC group. Serum levels of total cholesterol, LDL-cholesterol, and triglycerides, blood glucose and atherogenic index were significantly lower in the ARC than NC group. Furthermore, fatty liver and regional lipid accumultion in ob/ob mice were inhibited in the ARC group. The hepatic activities of superoxide dismutase, catalase and glutathione S-transferase were significantly higher in the ARC than NC group. Therefore, the anti-matabolic syndrome effects of the ARC group were higher than the AR group. In conclusion, these results indicated that CGJ added with arrowroot mediates its anti-obesity effects in ob/ob mice by improving lipid metabolism and antioxidant enzyme.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.6
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• pp.774-781
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• 2012

This study investigated dose-response effects of chlorogenic acid (CA) on glucose metabolism and the antioxidant system in streptozotocin (STZ)-induced diabetic mice with a high-fat diet (HFD). Male ICR mice were fed with a HFD (37% calories from fat) for 4 weeks prior to intraperitoneal injection with STZ (100 mg/kg body weight). Diabetic mice were supplemented with two doses of CA (0.02% and 0.05%, wt/wt) for 6 weeks. Both doses of CA significantly improved fasting blood glucose level, glucose tolerance and insulin tolerance without any changes in plasma insulin and C-peptide levels. Plasma leptin concentration was significantly higher in the CA-supplemented groups than in the diabetic control group. Both doses of CA significantly increased hepatic glucokinase activity and decreased glucose-6-phosphatase activity compared to the diabetic control group. The ratio of glucokinase/glucose-6-phosphatase was dose-independently higher in CA-supplemented mice than in diabetic control mice. CA supplementation dose-independently elevated superoxide dismutase and catalase activities, whereas it lowered lipid peroxide levels compared to the diabetic control mice in the liver and erythrocyte. These results suggest that low-dose CA may be used as a hypoglycemic agent in a high-fat diet and STZ-induced diabetic mice.

• The Korean Journal of Pesticide Science
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• v.9 no.3
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• pp.205-213
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• 2005

This study was conducted to evaluate the response of the selected aquatic plants to sulfonylurea(SU)-herbicides, which have been used widely in rice-cultivating areas in Korea, and the possibility of using them as bio-indicator species for biodiversity conservation. The aquatic plants identified in Kangwon province where butachlor have been used extensively for many years were much more varied than those in Chonnam where SU-herbicides have been used extensively for many years. The growth responses of Monochoria korsakowii, Marsilea quadrifolia and Salvinia natans to sulfonylurea herbicides such as bensulfuron-methyl(BSM) and pyrazosulfuron-ethyl(PSE), were much more sensitive than those to butachlor and molinate. The $GR_{50}$ values of BSM and PSE for M. korsakowii, M. quadrifolia and S. natans were relatively very low when compared with those of butachlor and molinate. The number of internode and dry weight of M. quadrifolia which was exposed to herbicides runoff from rice fields were seriously affected. The acetolactate synthase(ALS) activities of M. quadrifolia sensitively inhibited by BSM and PSE were, and $I_{50}$(Inhibition 50%) were 5.6 and 2.1 nM, respectively.

• The Korean Journal of Pesticide Science
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• v.15 no.3
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• pp.303-309
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• 2011

This study was carried out to investigate the evaluation of insecticidal efficacy and control effect in field of 10 environmental-friendly agricultural materials (EFAM) and seasonal occurrence at the vineyards to the Lycorma delicatula. L. delicatula nymphs occurred from late May to late August the highest early June (1st nymph) while adults occurred from late July to November with the highest peak of early October (preovipositing female) in 2010. Total population density of L. delicatula was the highest in early June. For the 3rd~4th nymphal instars and adults, EFAM (Spider, Jindikap-plus and Byejin-${\alpha}$) showed perfect insecticidal activity 2hour after treatment. Seoncho showed insecticidal activities 96.7% within 48 hour. Residual effects between EFAM showed 55.5% Spider at recommended concentration at 7 days after treatment(DAT), the other EFAM had low efficacy. Jindikap-plus and Spider showed 99% control value on nymphs in the field test at 3DAT, appeared control value more than 90% at 7DAT. Also, the control effects of Jindikap-plus and Spider were showed the adult mortality of 100% and 98.2% respectively at 3DAT, 90.6% and 84.6% respectively at 7DAT. Whereas the other EFAM had low efficacy. Jindikap-plus and Spider were excellent control effects compared with Acetamiprid WP.

• Korean Journal of Weed Science
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• v.30 no.3
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• pp.243-251
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• 2010

The study was conducted to evaluate the weed suppressing effects of two winter cover crops, Chinese milk vetch (CMV) and rye, in minor cereal fields in 2009. The suppressing activities of cover crops were due to the allelopathy of cover crops during weed emerging periods and competitive activity during vegetative growth of weeds. Weed suppression by cover crops were changed from 26.5 g $m^{-2}$, 0.1 g $m^{-2}$ and 0.2 g $m^{-2}$ in control, rye field and CMV field, respectively, on June 22 to 428.6 g $m^{-2}$, 1 g $m^{-2}$ and 403.9 g $m^{-2}$ in control, rye field and CMV field, respectively, on July 22. The weed dry weights were further changed to 656.3 g $m^{-2}$, 607.8 g $m^{-2}$ and 511.8 g $m^{-2}$ in control, rye field and CMV field, respectively, on August 22. The suppressing effect of cover crops also observed in minor cereals. Plant height and dry weight of sorghum was reduced to 68.7% and 33.6% of handweeding, respectively, on August 5. The reduction was recovered to 82.7% and 55.6% of control, respectively, on August 26. In addition, heading date of minor cereals was delayed by 7 days with rye.

• Korean Journal of Food Science and Technology
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• v.48 no.6
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• pp.590-596
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• 2016

This study was designed to investigate the antioxidant activities of the ethanol extracts (GBE) of grape branches (Campbell Early). The total polyphenol and flavonoid content of GBE was $201.42{\pm}4.16$ and $11.85{\pm}0.44mg\;GAE/g$, respectively. The antioxidant activity of GBE was measured using the ABTS and DPPH assays, and the $IC_{50}$ values were $45.60{\pm}0.09$ and $299.13{\pm}0.22$, respectively. GBE inhibited the production of pro-inflammatory mediators (NO, iNOS, $PGE_2$, COX-2, $IL-1{\beta}$, and IL-6) in lipopolysaccharide-stimulated RAW 264.7 macrophages in a dose-dependent manner. Moreover, GBE treatment significantly suppressed the production of $TNF-{\alpha}$ and IL-6 cytokines in phorbol 12-myristate 13-acetate plus calcium ionophore A23187-stimulated HMC-1 human mast cells. Furthermore, the administration of GBE markedly inhibited the scratching behavior induced by the compound 48/80 in ICR mice. These results suggested that GBE has potential as a therapeutic agent against inflammation and itch-related skin diseases.

• Journal of Life Science
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• v.23 no.8
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• pp.1010-1018
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• 2013

The greenhouse whitefly, Bemisia tabaci, is considered one of the most destructive pests of crops. In this study, we aimed to determine the optimal liquid culture conditions in shake flasks for maximal sporulation of Beauveria bassiana M130 using rice bran. The optimal initial pH for the spore production of B. bassiana using extracted rice bran medium was 5.2 and $28^{\circ}C$. The screening in shake flasks of carbon and nitrogen sources resulted in the identification of an optimal medium based on 0.5% $(NH_4)_2SO_4$, with extracted rice bran 8:1. Using this medium, a production level of $2.15{\times}10^9$ spores per ml was obtained after six days from culture inoculation at $28^{\circ}C$ in a rotary shaking incubator at 130 rpm. In addition, the specific activities of extracellular enzymes of chitinase and protease were $4,296{\mu}mol$ and $375{\mu}mol$, respectively. These results suggest that Beauveria bassiana M130 could be a bio-controller for the greenhouse whitefly.

• Korean Journal of Food Science and Technology
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• v.31 no.3
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• pp.823-830
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• 1999

The suppressing effects of crude extracts of three Korean teas, persimmon leaf tea extract (PLTE), green tea extract (GTE) and oolong tea extract (OTE), were studied on the induction of sister chromatid exchange (SCE) in cultured Chinese hamster ovary cells. When cells were treated with tea extract after mitomycin C (MMC) treatment, the frequency of MMC-induced SCEs were decreased at the high concentration $(1000\;{\mu}g/mL)$ of PLTE in the presence of S9 mix and at low concentrations $(20{\sim}80\;{\mu}g/mL)$ of PLTE in the absence of S9 mix, Whereas GTE and OTE showed suppressing effects on the MMC-induced SCEs at low concentrations $(10{\sim}20\;{\mu}g/mL)$ for OTE and $160\;{\mu}g/mL$ for GTE only in the presence of S9 mix. MMC-induced SCEs were decreased by post-treatment with each tea extracts with S9 mix in the G1 phase of the cell cycle. These results suggest that PLTE, GTE and OTE could have bio antimutagenic activities, and also suggest that PLTE might have unknown antimutagenic components which would be responsible for the inhibitory effect against direct acting mutagenicity.

• Research in Plant Disease
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• v.23 no.2
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• pp.177-185
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• 2017

Bacillus amyloliquefaciens LM11 was isolated from the feces of larvae of the rhino beetle and showed strong antifungal activities against various phytopathogenic fungi by producing biosurfactants. In this study, our overall goal was to determine relationship between biosurfactants produced from the LM11 strain and its role in growth inhibition of phytopathogenic fungi. Production and expression levels of B. amyloliquefaciens LM11 biosurfactants were significantly differed depending on growth phases. Transcriptional and biochemical analysis indicated that the biosurfactants of the LM11 strain were greatly enhanced in late log-phase to stationary phase. Inhibitions of phytopathogenic mycelial growth and spore germination were directly correlated (P<0.001, R=0.761) with concentrations of the LM11 cell-free culture filtrates. The minimum inhibitory surface tension of the culture filtrate of the B. amyloliquefaciens LM11 grown in stationary phase to inhibit mycelial growth of the phytopathogenic fungi was 38.5 mN/m (P<0.001, R=0.951-0.977). Our results indicated that the biosurfactants of B. amyloliquefaciens LM11 act as key antifungal metabolites in biocontrol of plant diseases, and measuring surface tension of the cell-free culture fluids can be used as an easy indicator for optimal usage of the biocontrol agents.