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http://dx.doi.org/10.3746/jkfn.2012.41.7.907

Piceatannol-Induced G1 Arrest of the Cell Cycle is Associated with Inhibition of Prostaglandin E2 Production in Human Gastric Cancer AGS Cells  

Choi, Yung-Hyun (Dept. of Biochemistry, Dongeui University College of Oriental Medicine, Dept. of Biomaterial Control (BK21 Program), and Anti-Aging Research Center & Blue-Bio Industry Regional Innovation Center, Dongeui University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.41, no.7, 2012 , pp. 907-913 More about this Journal
Abstract
Piceatannol (trans-3,4,3',5'-tetrahydroxystilbene) is a polyphenol detected in grapes, rhubarb, and sugarcane. Although recent experimental data revealed that this compound is known to exhibit immunosuppressive and antitumorigenic activities in several cell lines, the molecular mechanisms underlying anticancer activity are poorly understood. In the present study, we investigated possible further mechanisms by which piceatannol exerts its anti-proliferative action in cultured human gastric cancer AGS cells. Piceatannol treatment resulted in the inhibition of growth and G1 arrest of the cell cycle in a concentration-dependent manner, as determined by MTT assay and flow cytometry analysis. The induction of G1 arrest by piceatannol was associated with the modulation of cyclin-dependent kinases (Cdks) and cyclins, up-regulation of the expression of Cdk inhibitor p21 (WAF1/CIP1) in both transcriptional and translational levels, and the inhibition of phosphorylation of retinoblastoma proteins and E2F1 expression. In addition, piceatannol treatment caused a progressive decrease in the expression levels of cyclooxygenase (COX)-2 without significant changes in the levels of COX-1, which was correlated with a decrease in prostaglandin $E_2$ synthesis.
Keywords
piceatannol; G1 arrest; p21; COX-2;
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