• Asian-Australasian Journal of Animal Sciences
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• v.28 no.5
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• pp.620-631
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• 2015

A total of 59 lactic acid bacteria (LAB) strains were isolated from corn stover silage. According to phenotypic and chemotaxonomic characteristics, 16S ribosomal DNA (rDNA) sequences and recA gene polymerase chain reaction amplification, these LAB isolates were identified as five species: Lactobacillus (L.) plantarum subsp. plantarum, Pediococcus pentosaceus, Enterococcus mundtii, Weissella cibaria and Leuconostoc pseudomesenteroides, respectively. Those strains were also screened for antimicrobial activity using a dual-culture agar plate assay. Based on excluding the effects of organic acids and hydrogen peroxide, two L. plantarum subsp. plantarum strains ZZU 203 and 204, which strongly inhibited Salmonella enterica ATCC $43971^T$, Micrococcus luteus ATCC $4698^T$ and Escherichia coli ATCC $11775^T$ were selected for further research on sensitivity of the antimicrobial substance to heat, pH and protease. Cell-free culture supernatants of the two strains exhibited strong heat stability (60 min at $100^{\circ}C$), but the antimicrobial activity was eliminated after treatment at $121^{\circ}C$ for 15 min. The antimicrobial substance remained active under acidic condition (pH 2.0 to 6.0), but became inactive under neutral and alkaline condition (pH 7.0 to 9.0). In addition, the antimicrobial activities of these two strains decreased remarkably after digestion by protease K. These results preliminarily suggest that the desirable antimicrobial activity of strains ZZU 203 and 204 is the result of the production of a bacteriocin-like substance, and these two strains with antimicrobial activity could be used as silage additives to inhibit proliferation of unwanted microorganism during ensiling and preserve nutrients of silage. The nature of the antimicrobial substances is being investigated in our laboratory.

• Microbiology and Biotechnology Letters
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• v.17 no.5
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• pp.529-532
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• 1989

A strain of Streptomyces sp. (YS-221-B) extracellularly produced an inhibitory substance for $\alpha$-D-Glucosidase. The substance was purified 96-fold from culture filtrate by dialysis, heat treatment, adsorption on active carbon, Bio-Gel P-10 and Sephadex G-75 column chromatography with yield of 9.2%. The substance was stable in pH range from 7.0 to 11.0 at 37$^{\circ}C$, and a treatment at 10$0^{\circ}C$ for 20 min diminished only 15% of the original activity. The inhibitor was not inactivated by the treatment of $\alpha$-, $\beta$-amylases, glucoamylases, trypsin and chymotrypsin but inactivated by pyoteases from Streptomyces griseus and Tritirachium album.

• Tissue Engineering and Regenerative Medicine
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• v.15 no.6
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• pp.761-769
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• 2018

BACKGROUND: Bioprinting has recently appeared as a powerful tool for building complex tissue and organ structures. However, the application of bioprinting to regenerative medicine has limitations, due to the restricted choices of bio-ink for cytocompatible cell encapsulation and the integrity of the fabricated structures. METHODS: In this study, we developed hybrid bio-inks based on acrylated hyaluronic acid (HA) for immobilizing bio-active peptides and tyramine-conjugated hyaluronic acids for fast gelation. RESULTS: Conventional acrylated HA-based hydrogels have a gelation time of more than 30 min, whereas hybrid bio-ink has been rapidly gelated within 200 s. Fibroblast cells cultured in this hybrid bio-ink up to 7 days showed >90% viability. As a guidance cue for stem cell differentiation, we immobilized four different bio-active peptides: BMP-7-derived peptides (BMP-7D) and osteopontin for osteogenesis, and substance-P (SP) and Ac-SDKP (SDKP) for angiogenesis. Mesenchymal stem cells cultured in these hybrid bio-inks showed the highest angiogenic and osteogenic activity cultured in bio-ink immobilized with a SP or BMP-7D peptide. This bio-ink was loaded in a three-dimensional (3D) bioprinting device showing reproducible printing features. CONCLUSION: We have developed bio-inks that combine biochemical and mechanical cues. Biochemical cues were able to regulate differentiation of cells, and mechanical cues enabled printing structuring. This multi-functional bio-ink can be used for complex tissue engineering and regenerative medicine.

• Korean Journal of Medicinal Crop Science
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• v.27 no.6
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• pp.412-418
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• 2019

Background: Previously, studies have observed that the leaf extract of Cotoneaster wilsonii Nakai has potent antioxidant and anti-inflammatory activites. Therefore, further research was conducted to separate the active antioxidant and anti-inflammatory compounds in the leaf of the C. wilsonii. Methods and Results: The anti-oxidant effects were evaluated by analyzing the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, estimating the totla phenolic content (TPC) and total flavonoid content (TFC), and using reducing power assay method. The ethyl acetate (EtOAc) fraction demonstrated the highest DPPH radical scavenging activity (IC₅₀; 9.69 ㎍/㎖), and the highest TPC and TFC (345.98 mg·GAE/g, and 74.23 mg·QE/g). Moreover, it indicated the highest effects on nitric oxide production. Therefore, the active compound was separated using the EtOAc fraction, determined on the basis of spectral data, and identified as (+)-catechin. Conclusions: The first compound separated was made from the leaf of C. wilsonii was (+)-catechin, which produced potent anti-oxidant and anti-inflammatory effects.

• The Plant Pathology Journal
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• v.20 no.4
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• pp.293-296
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• 2004

During the screening of antiviral substances having inhibitory effects on Tobacco mosaic virus (TMV) infection on tobacco plants, we found a bacterial isolate KTB3, and identified it as Acinetobacter sp. which strongly inhibited the infection of TMV When the culture filtrate from KTB3 was applied on the upper surface of the Xanthi-nc tobacco leaves at the same time, or 24 hours before TMV inoculation, almost complete inhibition was achieved. Likewise, 86% inhibition was achieved, when the culture filtrate was applied on the underside of the leaves. In field trials, transmission of TMV from diseased seedlings to healthy ones during transplanting work was reduced by 92%, when the culture filtrate was sprayed onto the tobacco seedlings, cv. NC82, 24 hours before transplanting. No toxic effect was observed on the tobacco plants. Antiviral substance from the culture filtrate was purified by ethanol precipitation, dialysis, DEAE-cellulose, and Sephadex G75 gel column chromatography. The partially purified active material which showed positive color reaction to sugar and protein inhibited TMV infection by 60% at 1 ${\mu}$g/ml.

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.189.2-189.2
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• 2003

Seeds of Euryale ferox have been used for disorder of kidney, hysterorrhea of female and a tonic. In this study, in order to investigate the efficacy of antioxidant activity, the bio-activity guided fraction and isolation of physiologically active substance were performed. Roots, stems, flowers(seeds) were extracted with MeOH and each fractions were examined antioxidant activity by DPPH method. It was revealed that flowers(seeds) fration has significantly antioxidant activity. From flowers(seeds) frction, H$_2$O, 30%, 60%, 100% MeOH and acetone fractions were examined antioxidant activity by DPPH method. (omitted)

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.203.1-203.1
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• 2003

Cudrania tricuspidata have been used for anti-inflammatory, anti-hepatotoxic, anti-hypertensive and anti-diabetic activities. In this study, in order to investigate the efficacy of antioxidant activity, the bio-activity guided fraction and isolation of physiologically active substance were performed. H$_2$O, 30%, 60%, 100% MeOH and acetone fractions were examined antioxidant activity by DPPH method. It was revealed that 30%, 60%, 100% MeOH frations have significantly antioxidant activity. (omitted)

• Korean Journal of Exercise Nutrition
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• v.23 no.4
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• pp.26-31
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• 2019

[Purpose] Numerous epidemiological studies have shown that it is possible to prescribe exercise for neurodegenerative disease, such as Alzheimer's disease and Parkinson's disease. However, despite the availability of diverse scientific knowledge, the effects of exercise in this regard are still unclear. Therefore, this study attempted to investigate a substance, such as black chokeberry (Aronia melanocapa L.) that could improve the ability of the treatment and enhance the benefits of exercising in neurodegenerative diseases. [Methods] The cell viability was tested with 2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolim-5-carboxanilide and the cells were stained with ethidium homodimer-1 solution. The mRNA expression levels were evaluated by microarray. The active compounds of black chokeberry ethanolic extract (BCE) were analyzed by gas chromatography. The chemical shift analysis in the brain was performed using magnetic resonance spectroscopy. [Results] BCE treatment decreased hydrogen peroxide-induced L6 cell death and beta amyloid induced primary neuronal cell death. Furthermore, BCE treatment significantly reduced the mRNA levels of the inflammatory factors, such as IL-1α, Cxcl13, IL36rn, Itgb2, Epha2, Slamf8, Itgb6, Kdm6b, Acvr1, Cd6, Adora3, Cd27, Gata3, Tnfrsf25, Cd40lg, Clec10a, and Slc11a1, in the primary neuronal cells. Next, we identified 16 active compounds from BCE, including D-mannitol. In vivo, BCE (administered orally at a dosage of 50 mg/kg) significantly regulated chemical shift in the brain. [Conclusion] Our findings suggest that BCE can serve as a candidate for neurodegenerative disease therapy owing to its cyto-protective and anti-inflammatory effects. Therefore, BCE treatment is expected to prevent damage to the muscles and neurons of the athletes who continue high intensity exercise. In future studies, it would be necessary to elucidate the effects of combined BCE intake and exercise.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.1
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• pp.38-46
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• 2014

The purpose of this study was to investigate the effects of sowing time on the bio-active content of major soybean cultivars in a southern region of South Korea. Experiments were carried out in Naju, Jeonnam Province (latitude $35^{\circ}$ 04'N, longitude $126^{\circ}$ 54'E) from 2008 to 2010. The cultivars grown in summer included Saeol-kong and Tawon-kong and those grown in autumn included Taekwang-kong, Pungsannamul-kong, and Cheongja3, respectively. Sowing dates were May 15 and 30, June 15 and 30, and July 15 of each year. Daidzein content was increased in all the test cultivars grown from later sowing, and the contents of genistein and total isoflavone were increased in all the test cultivars except for Cheongja3. On the other hand, glycitein content showed no significant change in any of the test cultivars across the 5 sowing dates except for Tawon-kong. Cyanidin-3-glucoside (C3G) content was significantly increased in Tawon-kong and Cheongja3, and delphinidin-3-glucoside (D3G) and total anthocyanin contents were significantly increased in Tawon-kong. However, pelargonidin-3-glucoside (Pg3G) and petunidin-3-glucoside (Pt3G) contents showed no significant difference in plants sown across the 5 dates. Lutein content in Cheongja3 was between 2.7 and $3.0{\mu}g/g$ based on sowing date and did not vary significantly, while chlorophyll content increased with later sowing and was significantly higher in the cultivars sown on June 30 and July 15. Crude fat content was higher in Taekwang-kong and Cheongja3 when the cultivars were sown earlier; crude fat did not vary in the other test cultivars based on sowing date. Palmitic acid composition was not affected by sowing date in most of the test cultivars. Stearic acid was increased in Saeol-kong, Tawon-kong, and Cheongja3 with later sowing. Oleic acid and linoleic acid were significantly higher in Saeol-kong at an earlier sowing date. The composition of linolenic acid showed significantly increased dates in most of the test cultivars with later sowing. There were no significant differences in carbohydrate and crude protein content across 5 different sowing dates in most of the test cultivars. However, crude protein content was higher in Saeol-kong when sowing occurred earlier, and was higher in Tawon-kong at later sowing dates.

• Food Engineering Progress
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• v.13 no.4
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• pp.326-334
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• 2009

Blood coagulation and aggregation of platelet are crucial events in the pathogenesis of various ischemic diseases. The substance which can prevent blood coagulation and platelet aggregation was extracted from wood ear mushroom (Auricularia auricular-judae) and its anticoagulation activity was investigated. The dried A. auricular-judae was extracted with 0.1 N NaOH and its supernatant was further extracted with methanol and ethanol followed by $H_{2}O$. The resulting methanol soluble fraction showed significant antithrombotic activity in activated partial thromboplastin time, thrombin time, and prothrombin time assays with values of 100, 124, and 54 sec, respectively. This active substance was purified with DEAE-Sepharose CL6B and Sephacryl 400-HR and was found to be polysaccharide with the average molecular weight of over 150 kDa. This polysaccharide was xyloglucomannan of which the main component was mannose, and its anticoagulant activity was mostly mediated by inhibition of thrombin activity.