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Purification of Antithrombotic Material from Auricularia auricular-judae Extracts and Its Antithrombotic Activity  

Park, Young-Seo (Department of Food Science and Biotechnology, Kyungwon University)
Choi, Hyuk-Joon (BK bio Co., Ltd.)
Choi, Tae Hyun (Korea Institute of Radiological and Medical Sciences)
Publication Information
Food Engineering Progress / v.13, no.4, 2009 , pp. 326-334 More about this Journal
Abstract
Blood coagulation and aggregation of platelet are crucial events in the pathogenesis of various ischemic diseases. The substance which can prevent blood coagulation and platelet aggregation was extracted from wood ear mushroom (Auricularia auricular-judae) and its anticoagulation activity was investigated. The dried A. auricular-judae was extracted with 0.1 N NaOH and its supernatant was further extracted with methanol and ethanol followed by $H_{2}O$. The resulting methanol soluble fraction showed significant antithrombotic activity in activated partial thromboplastin time, thrombin time, and prothrombin time assays with values of 100, 124, and 54 sec, respectively. This active substance was purified with DEAE-Sepharose CL6B and Sephacryl 400-HR and was found to be polysaccharide with the average molecular weight of over 150 kDa. This polysaccharide was xyloglucomannan of which the main component was mannose, and its anticoagulant activity was mostly mediated by inhibition of thrombin activity.
Keywords
Auricularia auricular-judae; anticoagulant activity; polysaccharide;
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1 Ham SS, Kim DS, Lee DS. 1997b. Antimutagenic effect ofmethyl alcohol extracts from Auricularia auricula and Gyrophoraesculenta. Korean J. Food Sci. Technol. 29: 1281-1287   과학기술학회마을   ScienceOn
2 McGinnis DM, Outschoorn AS. 1991. Problems of componentactivities of heparin. Pharmacop. Forum 5: 2438-2441
3 Kim SS, Kim YP. 1995. Korean mushrooms. Yu-Pung Co. Ltd.,Seoul, Korea, p. 321
4 Lee SA, Jung KS, Shim MJ, Choi OC, Kim PK. 1981. The studyon anticancer component of Korea Basidiomycetes (II), Schizophyllumand Auricularia auricula-judae-judae. Korean Soc.Mycol. 9: 25-32   ScienceOn
5 Chang JS, Kim HJ, Bae JT, Park SH, Kim SE, Kim OM. 1998.Inhibition effects of Auricularia auricula-judae-judae methanolextract on lipid peroxidation and liver damage in benzo(a)pyrenetreatedmice. J. Korean Soc. Food Sci. Nutr. 27: 712-727   과학기술학회마을   ScienceOn
6 Merton RE, Thomas DP. 1987. Experimental studies on the relativeefficacy of dermatan sulphate and heparin as antithromboticagents. Thromb. Haemost. 8: 839-842   PUBMED   ScienceOn
7 Ham SS, Kim DH, Choi KP, Lee DS. 1997a. Antigenotoxiceffects of methyl alcohol extracts from Auricularia auricula andGyrophora esculenta. J. Korean Soc. Food Sci. Nutr. 26: 57-62   과학기술학회마을   ScienceOn
8 Nader HB, Dietrich CP. 1989. Natural occurrence and possiblebiological role of heparin. In: Heparin: chemical and biologicalproperties-clinical application. Lane DA, Lindahl U (eds) AcademicPress, London, Auckland. p. 81
9 Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. 1956.Colorimetric method for determination of sugars and relatedsubstances. Anal. Chem. 175: 595-603   DOI
10 Epstein FH. 1999. Vascular-bed: specific hemostasis and hypercoagulablestates. New Engl. J. Med. 340: 1555-1564   DOI   PUBMED   ScienceOn
11 Jaques LB. 1979. Heparin: an old drug with a new paradigm. Sci.206: 528-533   DOI   PUBMED   ScienceOn
12 Kenneth GM. 1997. Thrombosis: theoretical considerations. Am. J.Clin. Nutr. 65: 1657-1664   ScienceOn