Food Engineering Progress (산업식품공학)

Korean Society for Industrial Food Engineering (한국산업식품공학회)
권호 표지

Purification of Antithrombotic Material from Auricularia auricular-judae Extracts and Its Antithrombotic Activity

목이버섯 추출물로부터 항혈전물질의 정제와 항혈전효과

  • Received : 2009.10.26
  • Accepted : 2009.11.17
  • Published : 2009.11.30
⟨ Previous Next ⟩

Abstract

Blood coagulation and aggregation of platelet are crucial events in the pathogenesis of various ischemic diseases. The substance which can prevent blood coagulation and platelet aggregation was extracted from wood ear mushroom (Auricularia auricular-judae) and its anticoagulation activity was investigated. The dried A. auricular-judae was extracted with 0.1 N NaOH and its supernatant was further extracted with methanol and ethanol followed by $H_{2}O$. The resulting methanol soluble fraction showed significant antithrombotic activity in activated partial thromboplastin time, thrombin time, and prothrombin time assays with values of 100, 124, and 54 sec, respectively. This active substance was purified with DEAE-Sepharose CL6B and Sephacryl 400-HR and was found to be polysaccharide with the average molecular weight of over 150 kDa. This polysaccharide was xyloglucomannan of which the main component was mannose, and its anticoagulant activity was mostly mediated by inhibition of thrombin activity.

목이버섯으로부터 항혈전활성과 항혈소판응집활성을 지니는 물질을 추출하여 추출물의 혈행개선활성을 조사하였다. 건조 목이버섯을 0.1 N NaOH, methanol, ethanol 등의 용매를 사용하여 추출하여 각 추출물의 항혈전활성을 activated partial thromboplastin time, thrombin time과 prothrombin time 값으로 측정한 결과 methanol 용해성 분획이 각각 100, 124, 54 s로 조사한 분획 중에서 가장 높은 활성을 나타내었다. 활성 분획을 DEAE-Sepharose CL6B ion exchange column chromatography와 Sephacryl 400-HR gel permeation column chromatography로 정제하였으며 활성물질은 분자량이 150 kDa 이상인 다당류이며 mannose가 주요 구성당으로 되어 있는 xyloglucomannan의 복합다당체인 것으로 확인되었다. 정제된 다당류의 항혈전활성은 thrombin 활성을 저해하기 때문인 것으로 해석되었다.

Keywords

Acknowledgement

Supported by : 중소기업청, 경원대학교

References

  1. Chang JS, Kim HJ, Bae JT, Park SH, Kim SE, Kim OM. 1998.Inhibition effects of Auricularia auricula-judae-judae methanolextract on lipid peroxidation and liver damage in benzo(a)pyrenetreatedmice. J. Korean Soc. Food Sci. Nutr. 27: 712-727
  2. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. 1956.Colorimetric method for determination of sugars and relatedsubstances. Anal. Chem. 175: 595-603 https://doi.org/10.1021/ac60111a017
  3. Epstein FH. 1999. Vascular-bed: specific hemostasis and hypercoagulablestates. New Engl. J. Med. 340: 1555-1564 https://doi.org/10.1056/NEJM199905203402007
  4. Ham SS, Kim DH, Choi KP, Lee DS. 1997a. Antigenotoxiceffects of methyl alcohol extracts from Auricularia auricula andGyrophora esculenta. J. Korean Soc. Food Sci. Nutr. 26: 57-62
  5. Ham SS, Kim DS, Lee DS. 1997b. Antimutagenic effect ofmethyl alcohol extracts from Auricularia auricula and Gyrophoraesculenta. Korean J. Food Sci. Technol. 29: 1281-1287
  6. Jaques LB. 1979. Heparin: an old drug with a new paradigm. Sci.206: 528-533 https://doi.org/10.1126/science.386509
  7. Kenneth GM. 1997. Thrombosis: theoretical considerations. Am. J.Clin. Nutr. 65: 1657-1664
  8. Kim SS, Kim YP. 1995. Korean mushrooms. Yu-Pung Co. Ltd.,Seoul, Korea, p. 321
  9. Lee SA, Jung KS, Shim MJ, Choi OC, Kim PK. 1981. The studyon anticancer component of Korea Basidiomycetes (II), Schizophyllumand Auricularia auricula-judae-judae. Korean Soc.Mycol. 9: 25-32
  10. McGinnis DM, Outschoorn AS. 1991. Problems of componentactivities of heparin. Pharmacop. Forum 5: 2438-2441
  11. Merton RE, Thomas DP. 1987. Experimental studies on the relativeefficacy of dermatan sulphate and heparin as antithromboticagents. Thromb. Haemost. 8: 839-842
  12. Nader HB, Dietrich CP. 1989. Natural occurrence and possiblebiological role of heparin. In: Heparin: chemical and biologicalproperties-clinical application. Lane DA, Lindahl U (eds) AcademicPress, London, Auckland. p. 81