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http://dx.doi.org/10.7783/KJMCS.2019.27.6.412

Biological Activities and Separation of Active Substance of Extract and Fractions from Cotoneaster wilsonii Nakai Leaf  

Yoo, Nam Ho (Division of Bio-Resource Sciences, Kangwon National University)
Kim, Hee Kyu (Gangwondo Forest Science Institute)
Song, Jae Mo (Gangwondo Forest Science Institute)
Lee, Chan Ok (Gangwondo Forest Science Institute)
Park, Ju Hee (Lambio Institute, Kangwon National University,)
Park, Byung Jun (Division of Bio-Resource Sciences, Kangwon National University)
Choi, Yeong Bin (Division of Bio-Resource Sciences, Kangwon National University)
Baek, Young Sun (Division of Bio-Resource Sciences, Kangwon National University)
Hwang, Yeon Ji (Division of Bio-Resource Sciences, Kangwon National University)
Kim, Myong Jo (Division of Bio-Resource Sciences, Kangwon National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.27, no.6, 2019 , pp. 412-418 More about this Journal
Abstract
Background: Previously, studies have observed that the leaf extract of Cotoneaster wilsonii Nakai has potent antioxidant and anti-inflammatory activites. Therefore, further research was conducted to separate the active antioxidant and anti-inflammatory compounds in the leaf of the C. wilsonii. Methods and Results: The anti-oxidant effects were evaluated by analyzing the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, estimating the totla phenolic content (TPC) and total flavonoid content (TFC), and using reducing power assay method. The ethyl acetate (EtOAc) fraction demonstrated the highest DPPH radical scavenging activity (IC50; 9.69 ㎍/㎖), and the highest TPC and TFC (345.98 mg·GAE/g, and 74.23 mg·QE/g). Moreover, it indicated the highest effects on nitric oxide production. Therefore, the active compound was separated using the EtOAc fraction, determined on the basis of spectral data, and identified as (+)-catechin. Conclusions: The first compound separated was made from the leaf of C. wilsonii was (+)-catechin, which produced potent anti-oxidant and anti-inflammatory effects.
Keywords
Cotoneaster wilsonii Nakai; Anti-oxidant; Anti-inflammatory; Catechin;
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