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Isolation and Culture of Protoplasts from Embryonic Cotyledons of Pinus densiflora S. et Z. (소나무 자엽(子葉)을 이용(利用)한 원형질체(原形質體) 나출(裸出) 및 배양(培養))

  • Lee, Kyung Joon;Lee, Jae Soon;Youn, Yang;Lee, Suk Koo
    • Journal of Korean Society of Forest Science
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    • v.71 no.1
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    • pp.9-13
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    • 1985
  • Protoplasts were isolated from cotyledons of germinating Pinus densiflora S. et Z. seeds. The seeds were germinated for nine days, and excised embryonic cotyledons about 3-4mm long were incubated with Cellulase Onozuka R-10 and Macerozyme. After 8 hours of incubation, large number of viable protoplasts were isolated. Isolated protoplasts were cultured in a medium containing basal salts of $B_5$ medium, vitamines, amino acids, organic acid, sugars, and growth hormones. The first evidence of protoplast budding was observed after twelve hours in culture, and it suggested that high potential of the embryonic cotyledons for rapid cell division affected the early budding, rather than effect of culture medium was shown in twelve hours. The three- to four-cell stage was reached after three to four days of culture. Most cell divisions were achieved by additional buddings rather than equal binary cell division. No further cell division was observed beyond the four-cell stage. Protoplasts isolated from fully expanded cotyledons (germinated for 17 to 24 days) seldom initiated or failed to initiate cell division.

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Preparation of Al2O3 Thin Films by Atomic Layer Deposition Using Dimethylaluminum Isopropoxide and Water and Their Reaction Mechanisms

  • An, Ki-Seok;Cho, Won-Tae;Sung, Ki-Whan;Lee, Sun-Sook;Kim, Yun-Soo
    • Bulletin of the Korean Chemical Society
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    • v.24 no.11
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    • pp.1659-1663
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    • 2003
  • $Al_2O_3$ thin films were grown on H-terminated Si(001) substrates using dimethylaluminum isopropoxide [DMAl: $(CH_3)_2AlOCH(CH_3)_2$], as a new Al precursor, and water by atomic layer deposition (ALD). The selflimiting ALD process by alternate surface reactions of DMAI and $H_2O$ was confirmed from measured thicknesses of the aluminum oxide films as functions of the DMAI pulse time and the number of DMAI-$H_2O$ cycles. Under optimal reaction conditions, a growth rate of ~1.06 ${\AA}$ per ALD cycle was achieved at the substrate temperature of $150\;^{\circ}C$. From a mass spectrometric study of the DMAI-$D_2O$ ALD process, it was determined that the overall binary reaction for the deposition of $Al_2O_3\;[2\;(CH_3)_2AlOCH(CH_3)_2\;+\;3\;H_2O\;{\rightarrow}\;Al_2O_3\;+\;4\;CH_4\;+\;2\;HOCH(CH_3)_2]$can be separated into the following two half-reactions: where the asterisks designate the surface species. Growth of stoichiometric $Al_2O_3$ thin films with carbon incorporation less than 1.5 atomic % was confirmed by depth profiling Auger electron spectroscopy. Atomic force microscopy images show atomically flat and uniform surfaces. X-ray photoelectron spectroscopy and cross-sectional high resolution transmission electron microscopy of an $Al_2O_3$ film indicate that there is no distinguishable interfacial Si oxide layer except that a very thin layer of aluminum silicate may have been formed between the $Al_2O_3$ film and the Si substrate. C-V measurements of an $Al_2O_3$ film showed capacitance values comparable to previously reported values.

Activated Carbon Adsorption Characteristics of Multi-component Volatile Organic compounds in a Fixed Bed Adsorption Bed (고정층 흡착탑에서 다성분 휘발성 유기화합물의 활성탄 흡착 특성)

  • Cho, Jong Hoon;Lee, Sihyun;Rhee, Young Woo
    • Korean Chemical Engineering Research
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    • v.54 no.2
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    • pp.239-247
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    • 2016
  • This study aims to examine absorption characteristics of toluene, isopropyl alcohol (IPA), ethyl acetate (EA), and ternary-compounds, all of which are widely used in industrial processes, by means of four types of commercial activated carbon substances. It turned out that among the three types of volatile organic compounds, the breakthrough point of activated carbon and that of IPA, whose affinity was the lowest, were the lowest, and then that of EA and that of toluene in the order. With the breakthrough point of IPA, which was the shortest, as the standard, changes in the breakthrough points of unary-compounds, binary-compounds, and ternary-compounds were examined. As a result, it turned out that the larger the number of elements, the lower the breakthrough point. This resulted from competitive adsorption, that is, substitution of substances with a low level of affinity with those with a high level of affinity. Hence, the adsorption of toluene-IPA-EA and ternary-compounds require a design of the activated carbon bed based on the breakthrough of IPA, and in the design of activated carbon beds in actual industries as well, a substance whose level of affinity is the lowest needs to be the standard.

A Search Method for Components Based-on XML Component Specification (XML 컴포넌트 명세서 기반의 컴포넌트 검색 기법)

  • Park, Seo-Young;Shin, Yoeng-Gil;Wu, Chi-Su
    • Journal of KIISE:Software and Applications
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    • v.27 no.2
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    • pp.180-192
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    • 2000
  • Recently, the component technology has played a main role in software reuse. It has changed the code-based reuse into the binary code-based reuse, because components can be easily combined into the developing software only through component interfaces. Since components and component users have increased rapidly, it is necessary that the users of components search for the most proper components for HTML among the enormous number of components on the Internet. It is desirable to use web-document-typed specifications for component specifications on the Internet. This paper proposes to use XML component specifications instead of HTML specifications, because it is impossible to represent the semantics of contexts using HTML. We also propose the XML context-search method based on XML component specifications. Component users use the contexts for the component properties and the terms for the values of component properties in their queries for searching components. The index structure for the context-based search method is the inverted file indexing structure of term-context-component specification. Not only an XML context-based search method but also a variety of search methods based on context-based search, such as keyword, search, faceted search, and browsing search method, are provided for the convenience of users. We use the 3-layer architecture, with an interface layer, a query expansion layer, and an XML search engine layer, of the search engine for the efficient index scheme. In this paper, an XML DTD(Document Type Definition) for component specification is defined and the experimental results of comparing search performance of XML with HTML are discussed.

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Design of Naphtha Cracker Gas Splitter Process in Petlyuk Column (납사열분해 가스분리공정에서의 Petlyuk Column 설계)

  • Lee, Ju Yeong
    • Journal of the Korean Institute of Gas
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    • v.24 no.1
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    • pp.17-22
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    • 2020
  • Light Naphtha is distillated from crude oil unit and separated into the methane, ethylene and propylene by boiling point difference in sequence. This separation is conducted using a series of binary-like columns. This separation method is known that the energy consumed in the reboiler is used to separate the heaviest components and most of this energy is discarded as vapor condensation in the overhead cooler. In this study, the first two columns of the separation process are replaced with the Petlyuk column. A structural design was exercised by the stage computation with ideal tray efficiency in equilibrium condition. Compared with the performance of a conventional system of 3-column model, The design outcome shows that the procedure is simple and efficient because the composition of the liquid component in the column tray was designed to be similar to the equilibrium distillation curve. The performance of the new process indicates that an energy saving of 12.1% is obtained and the cost savings of 44 million won per day based on gross domestic product is reduced under same total number of trays and the initial investment cost is saved.

Classifying Sub-Categories of Apartment Defect Repair Tasks: A Machine Learning Approach (아파트 하자 보수 시설공사 세부공종 머신러닝 분류 시스템에 관한 연구)

  • Kim, Eunhye;Ji, HongGeun;Kim, Jina;Park, Eunil;Ohm, Jay Y.
    • KIPS Transactions on Software and Data Engineering
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    • v.10 no.9
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    • pp.359-366
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    • 2021
  • A number of construction companies in Korea invest considerable human and financial resources to construct a system for managing apartment defect data and for categorizing repair tasks. Thus, this study proposes machine learning models to automatically classify defect complaint text-data into one of the sub categories of 'finishing work' (i.e., one of the defect repair tasks). In the proposed models, we employed two word representation methods (Bag-of-words, Term Frequency-Inverse Document Frequency (TF-IDF)) and two machine learning classifiers (Support Vector Machine, Random Forest). In particular, we conducted both binary- and multi- classification tasks to classify 9 sub categories of finishing work: home appliance installation work, paperwork, painting work, plastering work, interior masonry work, plaster finishing work, indoor furniture installation work, kitchen facility installation work, and tiling work. The machine learning classifiers using the TF-IDF representation method and Random Forest classification achieved more than 90% accuracy, precision, recall, and F1 score. We shed light on the possibility of constructing automated defect classification systems based on the proposed machine learning models.

An Analysis for Influencing Factors in Purchasing Electric Vehicle using a Binomial Logistic Regression Model (Focused on Suwon City) (이항로지스틱 회귀모형을 이용한 전기차 구매 영향요인 분석 (수원시를 중심으로))

  • Kim, Sukhee;Jeong, Gahyung
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.38 no.6
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    • pp.887-894
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    • 2018
  • An electric vehicle is emerging as an alternative to the response of global climate change and sustainability. However, an Electric vehicle has not been popular due to the constraints such as its price or technical limitations. In order to analyze the effect of purchasing electric vehicles, this study conducted a binary logistic regression model that demonstrates the relation between purchasing and influencing variables. Variables which have high correlation were excluded from the model through the correlation analysis to prevent multicollinearity. Socio-economic variables such as the number of owned vehicles, sex, ages are not significant. On the other hand, Variables related to prices, charging and policy are found to have a significant to effect on the purchase of electric vehicles. In accordance with the model estimated result, it seems to be necessary to improve the charging incentives, or to provide electric car information and to expand opportunities for experience electric vehicles. The result is also expected to be helpful for spreading electric vehicles and formulating policies.

Effects of Yigong-san for the Treatment of Anorexia in Children: A Systematic Review and Meta-Analysis (소아 식욕부진에 대한 이공산(異功散) 치료의 효과 : 체계적 문헌고찰 및 메타분석)

  • Lee, Bo-ram;Ha, Da-jung;Huh, Tae-young;Park, Sang-eun;Lee, Sun-haeng;Chang, Gyu-tae
    • The Journal of Internal Korean Medicine
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    • v.43 no.4
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    • pp.542-558
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    • 2022
  • Objectives: The purpose of this study was to assess the effect of Yigong-san on anorexia in children by conducting a systematic review and meta-analysis of randomized controlled trials (RCTs). Methods: Eleven electronic databases were searched on May 30, 2022 to collect relevant studies. All studies published up to the search date were considered. RCTs reporting the effect of Yigong-san on the treatment of anorexia in children were included. The primary outcome was an improvement in clinical anorexia symptoms after treatment. In this meta-analysis, continuous and binary outcomes were assessed, and the data were presented as the mean difference and risk ratio with their 95% confidence intervals. The risk of bias was assessed using the Cochrane Collaboration's risk of bias tool. Results: A total of nine studies were included in this systematic review. The treatment group (Yigong-san only or Yigong-san plus conventional treatment) showed a statistically significant effect compared to the control group (conventional treatment only) in total effective rate (Yigong-san only: RR 1.26, 95% CI 1.17, 1.36, I2=0%; Yigong-san plus conventional treatment: RR 1.32, 95% CI 1.18, 1.47, I2=0%), clinical symptoms, some of the anthropometric outcomes, and biological markers related to appetite and growth in children with anorexia. No serious adverse events related to Yigong-san were reported. Conclusions: Yigong-san showed statistically significant effects as a treatment for anorexia in children. However, the number of studies included in the meta-analysis was insufficient, and the herbs contained in the Yigong-san used in the included studies were not standardized. Future research should focus on the implementation of methodologically robust clinical research.

Comparing the Effects of Regional Household Expenditure Burden on Childbirth Intention of Married Women: The Case of Capital and Non-Capital Regions (지역별 가계지출 부담이 기혼여성의 출산 의사에 미치는 영향: 수도권과 비수도권 비교를 중심으로)

  • Lee, Da-Eun;Seo, Wonseok
    • Journal of Cadastre & Land InformatiX
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    • v.51 no.2
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    • pp.151-168
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    • 2021
  • This study compared and analyzed the effect of the burden of household expenditure in the metropolitan and non-metropolitan areas on the intention to childbirth intention to married women using a panel logit model. To this end, this analysis targeted married women aged 25 to 39 who are highly likely to be fertile. The main results are as follows; First of all, it was confirmed that the economic power of spouse can be an important factor in the childbirth intention regardless of region. Second, it was found that the higher the satisfaction of marriage had a positive effect on the childbirth intention, and also higher the value that children must have, the higher the childbirth intention. Third, it was confirmed that the burden of household expenditure is the most important factor in the willingness to childbirth intention, excluding factors such as the number of existing children. In particular, the burden on education spending in both the capital region and non-capital region was found to be a key reason for the decrease in the childbirth intention. Lastly, the burden of household expenditure showed different effects on childbirth intention on depending on the region. Specifically, in the capital region, medical spending and loans had a greater impact, while, in the non-capital region, transportation and communication costs had a greater impact on childbirth intentions. Through the results, this study confirmed the implication that easing the burden on household expenditure is continuously necessary to enhance childbirth, and that discriminatory policy approaches are required depending on the area of residence.

A Study of Film Crews' Needs to Participate in Overseas Production : On the Basis of Binary Logistics Analysis and Marginal Effect Method (영화제작인력들의 해외활동 참여욕구에 관한 연구 : 이분형 로지스틱 분석 및 한계효과분석을 중심으로)

  • Lee, Hee-Jin
    • Journal of Korea Entertainment Industry Association
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    • v.13 no.4
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    • pp.27-37
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    • 2019
  • This study empirically identified and sought implications for the degree of desire by film crews to participate in overseas production and the factors affecting them, based on the recognition that the active participation of skilled film crews in overseas production and joint ventures can be an alternative to the poor treatment of domestic film industry sites and the reduction of job losses due to problems with career barriers. In order for respondents to verify research questions, binomial logistic and marginal effects analysis using SPSS and STATA, the data obtained by surveying 402 film crews operating in various areas. The notable findings are as follow: First, about 65%(263 people) of the respondents expressed their desire to participate in overseas activity, indicating that a considerable number of film crews are looking forward to opportunities with overseas productions. Second, major factors that have a positive effect on film crews' need to participate in overseas activities have been found to be professionality, artistic identity, Korean Wave persistence, and career barriers. In other words, the higher the professionality, the more clearly the identity of the artist, the more positive expectations of the possibility of the Korean Wave continuing, and the more serious the problem of career disability, the clearer was the desire to participate in overseas production and joint-work activities.