Journal of Korean Society of Forest Science (한국산림과학회지)
- Volume 71 Issue 1
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- Pages.9-13
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- 1985
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- 2586-6613(pISSN)
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- 2586-6621(eISSN)
Isolation and Culture of Protoplasts from Embryonic Cotyledons of Pinus densiflora S. et Z.
소나무 자엽(子葉)을 이용(利用)한 원형질체(原形質體) 나출(裸出) 및 배양(培養)
- Lee, Kyung Joon (College of Agriculture, Seoul National Univ.) ;
- Lee, Jae Soon (Institute of Forest Genetics) ;
- Youn, Yang (Institute of Forest Genetics) ;
- Lee, Suk Koo (Institute of Forest Genetics)
- Received : 1985.08.08
- Published : 1985.12.30
Abstract
Protoplasts were isolated from cotyledons of germinating Pinus densiflora S. et Z. seeds. The seeds were germinated for nine days, and excised embryonic cotyledons about 3-4mm long were incubated with Cellulase Onozuka R-10 and Macerozyme. After 8 hours of incubation, large number of viable protoplasts were isolated. Isolated protoplasts were cultured in a medium containing basal salts of
침엽수류(針葉樹類)의 원형질체(原形質體) 배양(培養)의 가능성(可能性)을 시험(試驗)하기 위하여 소나무 종자(種子)의 자엽(子葉)에서 원형질체(原形質體)를 나출(裸出)하여 배양(培養)을 시도(試圖)하였다. 9일(日)동안 발아(發芽)시킨 채종원산(採種園産) 소나무 종자(種子)에서 길이가 3~4mm 가량 되는 자엽(子葉)을 잘라내서 Cellulase Onozuka R-10과 Macerozyme을 첨가(添加)한 효소용액(酵素溶液)에서 8시간(時間) 처리(處理)하여, 활성(活性)이 높은 원형질체(原形質體)를 대상(大量)으로 얻었다. 나출(裸出)된 원형질체(原形質體)를