• 생약학회지
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• 제1권2호
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• pp.31-32
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• 1970

Silica gel column chromatography on the ether extract from the fruits of Angelica koreana $M_{AXIMOWICZ}$ gave two kinds of crystalline products of coumarin. The following furocoumarins-imperatorin and bergapten has been identified by elemental analysis, IR, NMR spectra and physico-chemical tests.

• 대한화장품학회지
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• 제13권1호
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• pp.1-6
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• 1987

We have confirmed the phototoxicity of orange flower absolute, studied the phototoxic ingredient and developed a method to eliminate it. To confirm the phototoxicity, we tested French orange flower absolute, Moroccan absolute and Egyptian absolute with Hartley strain guinea Pig under UV irradiation ranging from 320400 nm supplied with fluorescent lamps. Using a combination of isolation techniques including HPLC and IR, we were able to confirm that the phototoxic agent was bergapten. The development of a non-phototoxic orange flower absolute using ion exchange chromatography is described.

• Applied Biological Chemistry
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• 제38권4호
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• pp.293-296
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• 1995

무화과잎의 메탄올추출물을 용매분획하여 얻은 분획중 산성 분획과 페놀성 분획이 항미생물 활성이 강한 것으로 나타났고 페놀성 분획이 산성 분획보다 항미생물 활성이 더 강하여 페놀성 분획중의 항미생물 활성물질을 동정하기 위하여 먼저 silica gel column chromatography, RP-8 column chromatography 및 preparative liquid chromatography를 행하여 정제하였고 정제된 항미생물 활성물질을 IR, $^{1}H-NMR$ 및 MS에 의한 기기분석을 통하여 동정한 결과 각각 psoralen, bergapten, umbelliferone 및 ${\beta}$-sitosterol로 구명되었다.

• Natural Product Sciences
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• 제23권2호
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• pp.97-102
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• 2017

Fourteen compounds were isolated from the stem of Angelica polymorpha. On the basis of spectral data, these compounds were identified as isoimperatorin (1), phellopterin (2), bergapten (3), xanthyletin (4), cnidilin (5), geijerine (6), (-)-3'-acetyl hamaudol (7), 7-demethylsuberosine (8), dehydrogeijerin (9), (-)-hamaudol (10), (+)-visamminol (11), divaricatol (12), scopoletin (13), and decursidate (14), respectively. Among them, compounds 4-6, 8, 9, 13, and 14 were isolated for the first time from A. polymorpha. Dehydrogeijerin (6) and geijerin (9) were isolated for the first time from genus Angelica. All isolates tested for inhibitory activity against acetylcholinesterae. Compounds 1 to 13 showed acetylcholinesterase inhibitory activity with $IC_{50}$ values ranging from 1.4 to $37.5{\mu}M$.

• 생약학회지
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• 제7권4호
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• pp.233-235
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• 1976

Substances $I{\sim}VII$ are isolated from the root of Glehnia littoralis which has been often used as a subsitute of Bang-Poong in Korea. White needle crystalline substance of $C_{29}H_{50}O$, m.p. $137{\sim}139^{\circ}C$ was confirmed as ${\beta}-sitosterol$ through elemental analysis, derivative synthesis, IR and physicochemical tests. Moreover, it was concluded that white needle crystal of $C_{12}H_{8}O_{4}$, m.p. $188{\sim}189^{\circ}$ was confirmed as bergapten, through a contrast test with standard substance and by UV and IR-spectra. It was found that substance III and VII coincided with the Rf values of imperatorin and umbelliferone and to its colors under the UV-ray, and its similarity to UV-spectrum can be recognized, but their melting points are remarkedly different respectively.

• Natural Product Sciences
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• 제11권4호
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• pp.229-232
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• 2005

The repeated column chromatographic separation of the EtOH extract of Portulaca oleracea afforded seven compounds. The structures of these isolates were identified as bergapten (1), umbelliferone (2), daidzein (3), genistein (4), protocatechuic acid (5), ferulic acid (6), and gallic acid (7) by the analysis of physico-chemical and spectral data. Their antioxidant effect on free radical scavenging was evaluated in the DPPH assay.

• 생약학회지
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• 제1권4호
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• pp.119-124
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• 1970

Evodia daniellii $H_{EMSLEY}$, a plant belonging to Rutaceae, is cultivated in Korea and used as a folkmedicine for gastric inflammation, extermination of noxious insects, and headache. The seed oil of this plant also has been used in various diseases, for example, dermatitis, scabies and so forth. From the barks, fruit peels, and seed oil of Evodia daniellii $H_{EMSLEY}$, four crystalline compounds were isolated. Three compounds except one were characterized as methyl sinapate, bergapten, and evodiamine by m.p. determination, elemental analysis, UV, IR, NMR spectra and mass analysis. Even though methyl sinapate was a known synthetic compound, it was not yet reported as a natural product. From the seed oil, unsaponifiable fraction was separated and was analyzed. It was considered to be consisted with sterols, hydrocarbons and tocopherols.

• 생약학회지
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• 제51권3호
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• pp.158-162
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• 2020

Three coumarins, one chromone, and a phytosterol were isolated from the stem of Ostericum koreanum. Basis on the spectral data, we determined to be the structures of isolated compounds as bergapten, xanthotoxin, auraptenol, hamaudol, and mixture of phytosterols. Auraptenol was isolated for the first time from this plant.

• Archives of Pharmacal Research
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• 제26권6호
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• pp.449-452
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• 2003

Systematic fractionation of Angelica gigas roots led to the isolation of linear furano(pyrano)coumarins such as bergapten (1), decursinol angelate (2), decursin (3), nodakenetin (4) and nodakenin (5). The antibacterial activities of those compounds against pathogenic bacteria were investigated. Among the compounds tested, decursinol angelate (2) and decursin (3) exhibited significant antibacterial activity against Bacillus subtilis with the minimum inhibitory concentrations (MICs) of 50 and $12.5{\;}{\mu}g/mL$, respectively.

• Archives of Pharmacal Research
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• 제22권3호
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• pp.324-326
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• 1999

Four coumarins were isolated from chloroform extract of the root of Peucedanum japonicum and identified as praeruptorin A (1), xanthotoxin (2), psoralen (3) and bergapten (4) on the basis of spectroscopic methods. The inhibitory activities of these coumarins on monoamine oxidase prepared by mouse brian were tested. The IC50 values of them were shown to be $27.4{\mu}M$ (1), $40.7{\mu}M$ (2), $35.8{\mu}M$ (3), and $13.8{\mu}M$ (4), in vitro.