Applied Biological Chemistry
- Volume 38 Issue 4
- /
- Pages.293-296
- /
- 1995
- /
- 2468-0834(pISSN)
- /
- 2468-0842(eISSN)
Purification and Identification of Antimicrobial Substances in Phenolic Fraction of Fig Leaves
무화과잎 페놀성 분획중의 항미생물 활성물질의 정제 및 동정
-
Kang, Seong-Kuk
(Department of Food Science and Technology, Chonnam National University) ;
- Chung, Dong-Ok (Central Research Lab., Il Yang Pharmaceutical Company) ;
- Chung, Hee-Jong (Department of Food Science and Technology, Chonnam National University)
- Published : 1995.08.31
Abstract
Fig leaves were extracted with methanol and then fractionated with ethyl acetate and various buffers to get active fractions and determined the antimicrobial activities. The acidic and phenolic fractions fractionated from the methanol extract of fig leaves showed the strong antimicrobial activities, but the basic and neutral fractions did not show any activities. The degree of antimicrobial activities of phenolic fraction against tested bacteria was higher than those of acidic fraction, but these against yeasts and mold were almost equivalent to those of acidic fraction. Especially, phenolic fraction was mostly affected on Staphylococcus aureus and Pseudomonas aeruginosa. Four antimicrobial substances purified from the phenolic fraction which showed the strongest antimicrobial activities among the fractions from fig leaves, were identified as psoralen(
무화과잎의 메탄올추출물을 용매분획하여 얻은 분획중 산성 분획과 페놀성 분획이 항미생물 활성이 강한 것으로 나타났고 페놀성 분획이 산성 분획보다 항미생물 활성이 더 강하여 페놀성 분획중의 항미생물 활성물질을 동정하기 위하여 먼저 silica gel column chromatography, RP-8 column chromatography 및 preparative liquid chromatography를 행하여 정제하였고 정제된 항미생물 활성물질을 IR,