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Objective Identification of Human Sperm Hyperactivation by Computerized Sperm Motion Analysis (Computerized Sperm Motility Analyzer를 이용한 Human Sperm의 Hyperactivated Motility의 객관적 관찰에 관한 연구)

  • Lee, Hee-Kyung;Lee, Chan;Kim, Hyun-Sook;Kim, Young-Tae;Kim, Sun-Haeng;Ku, Pyoung-Sahm
    • Clinical and Experimental Reproductive Medicine
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    • v.21 no.1
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    • pp.1-11
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    • 1994
  • The occurrence and time course of capacitation, acrosomal loss, and hyperactivated motility require quantitative definition in order to characterize fertile human sperm. Recently the method has been developed to estimate the quality of spermatozoa by using kinematic parameters such as curvilinear velocity(VCL), average path velocity(VAP), linearity(LIN), straightness(STR), amplitude of lateral head displacement(ALH), and beat cross frequence(BCF) from Computer Assisted Sperm Analysis (CASA). In this study, using the Hamilton Thorn motility analyzer HTM 2030(Hamilton Thorn Research, Beverly, MA), we attempted to identify the spermatozoa with hyperactivated motility (HA) objectively and to monitor hyperactivation of human spermatozoa during incubation in capacitating media and after treatment of calcium ionophore as compared with acrosome status. And we examined whether HA are related to the result of SPA. Semen samples obtained from 16 healthy men were prepared by swim up technique and preincubated in a capacitating media(modified BWW medium) for 5 hours and treated with calcium ionophore solution. The acrosome reaction was detected with PSA-FITC labelling of the acrosome and in vitro sperm ferilizing capacity was assessed by the zona free hamster ovum penetration assay (SPA). The incidence of hyperactivated sperm was 2.6% in fresh semen, 14.3% of the swim up population, 13.7% after 5h of incubation. Significant increase of percentage of hyperactivated sperm was observed after the incubation (p<0.05) but after treatment, no significant changes of percentage of hyperactivated sperm(l1.8%) in contrast to significant rise in the percentage of acrosome reacted cells. Correlation analysis failed to show any significant relationship between the percentage of sperm with HA and SPA score. In conclusion, although no direct correlations were found between the results of SPA and HA, hyperactivation of sperm is associated with capacitation and monitoring hyperactivated sperm will be expected as a method of evaluating the functional quality of sperm such as SPA.

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A Study on Independent Rhythm Technique of Synthesizer & Percussions - Focusing on the Sting's "Nothing Like The Sun" Album - (신디사이저와 퍼커션의 독자적인 리듬기법 연구 - Sting의 "Nothing Like The Sun" 앨범을 중심으로 -)

  • Ohm, Soo-Han
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.12
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    • pp.327-332
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    • 2016
  • Sting (Gordon Sumner) is a British musician and singer-songwriter. He was the principal songwriter, lead singer, and bassist for the new wave rock band 'The Police' from 1977 to 1984, before launching a solo career in 1985. With his stylish and sophisticated sensibility and musical spectra, he achieved 100 million album sales. In this thesis, after he disbanded the group THE POLICE, his career as a solo artist with his wide variety of musical colors has been acknowledged for his reputation over more than 30 years. Therefore, I analyze 'Sting' of music included in 'Straight To My Heart' and 'Be Still My Beating Heart' in. Songs in indicate that the drum part the tunes that there is in a record doesn't break away from the basic way of music. The role of the whole rhythm division through the various percussions or can see a synthesizer than the role as the cord musical instrument that there are lots of tunes placing a great deal of weight on the role as the rhythmic musical instrument more. The unique instrument-based rhythm of the early Sting and role dispensation create a unique sound that is quite different from what the musician subsequently recorded. The feature and rhythmic characteristic, which is the arrangement that appeared outstandingly in this record, I am trying to explain about the way in which I lead the basic flow of the tune through the rhythm division of the other musical instruments which I split that is, the drum beat and break away from a rhythm from the divided general way and include a percussion.

The Comparative Analysis of EMG Activities on the Lower Limb Muscles during Power Walking and Normal Walking (파워보행과 일반보행 시 하지근의 근전도 비교 분석)

  • Gi, Se-Joon;Chae, Woen-Sik;Kang, Nyeon-Ju;Jang, Jae-Ik;Yoon, Chang-Jin
    • Korean Journal of Applied Biomechanics
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    • v.18 no.4
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    • pp.125-133
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    • 2008
  • The purpose of this study was to compare EMG activities on the lower limb muscles during power walking and mormal walking. Seventeen subjects who have no known musculoskeletal disorders performed walking exercise at a cadence of 140 beats/min. After surface electrodes were attached to rectus femoris, vastus medialis, vastus lateralis, biceps femoris, tibialis anterior, medial gastrocnemius, averageed IEMG and peak IEMG, were measured. The result showed that the power walking did influence the averaged IEMG and peak IEMG. The EMG activity of the quadriceps during power walking was significantly higher than the corresponding values in normal walking during most phases. The averaged IEMG and peak IEMG of gastrocnemius muscles at the end of the double limb stance increased significantly when going from normal walking to power walking. The results indicate that power walking had greater effect on EMG activities on the lower limb muscles and demonstrate that the wide range of benefits can be obtained from power walking in respect to health and fitness. This study suggests that power walking has the potential to improve aerobic fitness and assist in weight management.

Characteristics of Individuals Seeking Comprehensive Health Check-ups (종합 건강검진을 원하는 피검진자의 건강특성에 관한 연구)

  • 성미혜
    • Journal of Korean Academy of Nursing
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    • v.27 no.3
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    • pp.563-576
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    • 1997
  • This study was carried out to identify basic data for more efficient operation of comprehensive health check-up centers in the national health care system. The researcher reviewed and analyzed the main symptoms of the subjects according to sex, age, occupation and positive rate in the screening tests of a comprehensive health check-up, performed at one comprehensive health check-up center in located in a hospital. The subjects were 512 persons who had a check-up at the comprehensive health check-up, center in K university hospital in Seoul from October 2, 1996 to March 30, 1997. A questionaire developed by the researcher to obtain subject's general characteristics, main symptoms, medical examination, diagnosed disease was used as the tool for the study. The researcher analyzed the data with SPSS PC/sup +/ program. : the distribution of the subject's general characteristics and main symptoms was described with percentages and the difference between main symptoms and screening tests analyzed with X²-test, t-test, ANOVA. The result of this study are as follows : 1. For the distribution of diagnosis in males. liver disease was most frequent and hypertension was second : in females, hypertention and liver disease were not common in that order. 2. The most common complaints were indigestion in the digestive system, sputum in the respiratory system, irregular heart beat in the cardiovascular system, frequent urine in the urinary system vertigo in the hematologic system. 3. The main symptoms according to sex were for females, a statistically siguificant higher rate of complaints in the digestive system, cardiovascular system, urinary system, hematologic system, musculoskeletal, mental & nervous system as compared to males. 4. The main symptoms according to age were that in the the second decade there wara higher rate of complaints in the digestive system, and cardiovascular system than in the older age groups : and in the fifth decade more in the musculoskeletal system. 5. The main symptoms according to occupation were that formers and fishers had a higher rate of complaints in the mental and nervous system as compared to other occupation groups : and office workers, in gynecology. 6. The main symptoms according to diagnosis, were symptoms of the digestive system, frequent in liver disease : symptoms of the cardiovascular system in allergic disease. 7. The screening tests which showed the highest rates were UGI /FGS, PFT : In males a higher rate of abnormality was seen in abdominal ultrasono- graphy and in, females, in mammography. 8. In screening tests according to sex, males showed a higher positive rate for chest X-rays, and LFT, and females, in mammography, pap smear, CBC. 9. In screening tests according to age, higher positive rates of obesity, high blood pressure, chest X-ray, mammography, pap smear, FBS, CBC, urine test were seen in the fifth decade : PFT and abdominal ultrasonography in the third decade. The subjects who wanted comprehensive health check-up were not asymptomatic but had an illness with several symptoms. It is found that the most frequent diagnosed diseases were hypertention and liver disease. So proper observations must be done for them. Comprehensive health check-ups should be changed to a comprehensive examination including treatment beyond examination for those subjects with positive results.

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Advanced Calendar Queue Scheduler Design Methodology (진보된 캘린더 큐 스케줄러 설계방법론)

  • Kim, Jin-Sil;Chung, Won-Young;Lee, Jung-Hee;Lee, Yong-Surk
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.34 no.12B
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    • pp.1380-1386
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    • 2009
  • In this paper, we propose a CQS(Calendar Queue Scheduler) architecture which was designed for processing multimedia and timing traffic in home network. With various characteristics of the increased traffic flowed in home such as VoIP, VOD, IPTV, and Best-efforts traffic, the needs of managing QoS(Quality of Service) are being discussed. Making a group regarding application or service is effective to guarantee successful QoS under the restricted circumstances. The proposed design is aimed for home gateway corresponding to the end points of receiver on end-to-end QoS and eligible for supporting multimedia traffic within restricted network sources and optimizing queue sizes. Then, we simulated the area for each module and each memory. The area for each module is referenced by NAND($2{\times}1$) Gate(11.09) when synthesizing with Magnachip 0.18 CMOS libraries through the Synopsys Design Compiler. We verified the portion of memory is 85.38% of the entire CQS. And each memory size is extracted through CACTI 5.3(a unit in mm2). According to the increase of the memory’sentry, the increment of memory area gradually increases, and defining the day size for 1 year definitely affects the total CQS area. In this paper, we discussed design methodology and operation for each module when designing CQS by hardware.

Rapping as a Means of Improving Self-Expression: A Case Study of Adolescent Survivors of Childhood Cancer (소아암 완치 청소년의 자기표현 경험을 위한 랩 만들기 사례)

  • Choi, Jieun
    • Journal of Music and Human Behavior
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    • v.16 no.2
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    • pp.27-51
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    • 2019
  • This case study aimed to investigate changes in self-expression following participation in a rap making program with adolescent survivors of childhood cancer. The rap making program was constructed based on the contextual support music therapy model. Three adolescent survivors of childhood cancer participated in six 80-minute individual sessions. During the sessions, each participant engaged in the following tasks: song discussion, lyric creation, and rapping over a selected beat. At pre and posttest, the Self-Expression Scale was completed by participants. Their verbal expressions lyrics were observed during the sessions, and individual interviews with the participants were conducted at the completion of the program. The results demonstrated that the mean rating of the Self-Expression Scale increased after the rap making intervention. Analysis of the participants' verbal expressions and lyrics demonstrated that participants were experiencing difficulties adjusting to school that they wanted to resolve. Furthermore, the analysis of the interviews at posttest found that participants experienced positive changes in self-perception, self-expression, and expectations for their future, compared to the pretest when the participants expressed negative self-perceptions due to difficulties in interpersonal relationships at school and physical limitations. This indicates that rap making can be an effective resource for providing this population with the means to recognize positive attributes about themselves and improve self-expression.

Out-of-Hospital Resuscitation of Cardiac Arrest by 119 Emergency Medical Service System (119구급대에 의해 소생한 병원 전 심장정지 환자 1례)

  • Yun, Hyeong-Wan;Lee, Jae-Min;Jung, Ji-Yeon
    • Fire Science and Engineering
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    • v.24 no.5
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    • pp.142-149
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    • 2010
  • The aim of this study was to report the out-of-hospital resuscitation of cardiac arrest along with literature consideration. CPR is technology of resuscitating patient by maintaining oxygen supply to organ, by preventing progression from clinical death to biological death, and by recovering heart beat and circulation, through circulatory support of mechanical ventilation. It is not what every patient with cardiac standstill is revived even if being implemented CPR. Patient's survival rate is decided by how quickly and correctly CPR was executed. A patient, who wasn't witnessed the cardiac arrest on the field, was performed 5-cycle CPR for 2 minutes on the field before being transferred to hospital, and was allowed to be used AED. A person, who was observed, is recommended to be used AED immediately, and then is said to be needed ACLS (advanced cardiac life support). In the Out-of-Hospital stage, it is rare in a case that 119 Emergency Medical Service System transfers by being ROSC and in a case of executing ACLS. Cardiac arrest was witnessed on the field, but CPR wasn't executed. First-aid staff executed CPR after arriving, and led to ROSC on the field with ACLS, there by having experienced 1 case of a patient's surviving to leave hospital by being ROSC on the field.

Effects of Incubation and Thawing Temperature on Frozen-thawed Stallion Epididymal Spermatozoa (말의 정소상체 정자의 동결 후 해동 온도 및 Incubation의 효과)

  • Kim, Keun-Jung;Lee, Kyung-Bon;Lee, Ji-Hye;Kim, Eun-Young;Han, Kil-Woo;Park, Kang-Sun;Kim, Min-Kyu
    • Journal of Embryo Transfer
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    • v.28 no.3
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    • pp.297-302
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    • 2013
  • Cryopreservation of epididymal spermatozoa offers a potential tool for rescuing genetic material from males of genetically elite populations. Castration, catastrophic injury, sudden death or any other event that makes semen collection or mating impossible may prematurely terminate a stallion reproduction. Stallion epididymal spermatozoa vary widely in the loss of progressive motility, acrosomal integrity, and viability during freezing and thawing. The objective of this work was to investigate the effect of (1) freezing package types on cryopreservation efficiency, (2) thawing temperatures (37, 56 or $70^{\circ}C$) on Computer Assisted Sperm Analysis (CASA) parameters and (3) post-thawing incubation time (0, 1, 2 or 4h) on castrated stallion epididymis. Post-thawed sperm motility ranged between 59.69% and 64.28% ($56^{\circ}C$ and $37^{\circ}C$) in various thawing temperatures. When stallion epididymis sperm was frozen, straw was better than freezing tube on VCL (Velocity of Curvilinear Line) and VAP (Velocity of Average Path) parameter. Higher percentage of motility was observed at $37^{\circ}C$ thawing temperature even though no significant difference was observed among various temperatures. The motility, VCL, ALH (Amplitude of Lateral Head displacement), VAP, BCF (Beat-Cross Frequency) and STR (Straightness index) parameter of post-thawed sperm were significantly decreased by increasing the incubation time for all thawing temperatures. The present study showed that type of freezing package (Straw vs. Freezing tube) was not significantly different on cryopreservation efficiency. Furthermore, stallion epididymal spermatozoa frozen-thawed at $37^{\circ}C$ for 1 min resulted the highest proportion of motility and velocity movement. In addition, motility and viability of frozen-thawed stallion epididymal spermatozoa were also decreased by incubation.

A Study on the Experience of Back Pain and Developmental Factors of Male High School Students in an Urban area (일 도시지역 남자고교생들의 요통경험과 발생요인에 관한 연구)

  • Chung, Seung-Hee;Cho, Young-Shin
    • Journal of the Korean Society of School Health
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    • v.12 no.2
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    • pp.321-337
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    • 1999
  • The purpose of this study was to obtain basic data for the prevention and control of adolescent back pain through analyzing connections between study environments and physical posture. The subjects were 960 male general high school students in the Chonju area and the data were collected by a self-reported questionnaire from Mar. 17 to Mar. 22, 1999. The collected data were analyzed by a frequency, chi-square and t-test using an SPSS program. The results are as follows; 1) The experience rate of back pain perceived by subjects was 67.5% and by each grade: 1st-27.5%, 2nd-35.4%, 3rd-37.1%. The relationship between grades and the experience of back pain didn't show any significant difference. 2) The causes of back pain perceived by subjects such as 'postures are not good' was 56.7%, 'sitting too much time in a chair' was 39.1%, and 'too severe exercise' was 32.4%. 27.8% had back pain first during the 3rd grade of middle school, and 23.9% had it first during the 1st grade of high school. 3) Intensity of subjects' back pain spread from 'moderate' at 49.6%, to 'severe' at 16.4%. Concerning the frequency of back pain, 58.6% said it was 'irregalar'. 4) Among interventions to deal with back pain: 'move by exercising my back or ask friends to beat my back' was 41.0%, 'just bear it' was 23.1%, and at home 'don't have 'any treatment' was ranked first, at 54.9%. 5) The relationship between subjects' general characteristics and back pain experiences: height (t=-1.99, P=.046), sitting/height (t=-2.61, P=.009), self-perceived condition of health (${\chi}^2=23.530$, P=.000), family history (${\chi}^2=43.903$, P=.000) showed significant difference, but the kinds of transportation, sleeping postures, sleeping method and smoking didn't show significant differences. 6) The relationship between subjects' learning environment and back pain experiences, the height of students' desk and chair showed significant difference (${\chi}^2=23.054$, P=.000), but the sitting time didn't show significant difference. 7) The relationship between the characteristics of subjects' physical postures and back pain experiences: standing postures (${\chi}^2=15.105$, P=.001), and sitting postures (${\chi}^2=20.264$X2, P=.001) showed significant difference, but lifting postures didn't show significant difference.

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A Case Study on Art Therapy for the Behavior modification of Elementary School Children (초등학교 아동의 문제행동 수정을 위한 미술치료 사례 연구)

  • Shin, Hwa-Jeong
    • The Korean Journal of Elementary Counseling
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    • v.7 no.1
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    • pp.227-248
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    • 2008
  • This study aims to examine the effects of art therapy on challenging behavior of schoolchildren. For this purpose, I selected one class of 4th year student of S primary school, located at Jeongeup city, Jeonlabukdo, and selected 4 study subjects through examination of K-CBCL children. juvenile behavior evaluation scale, characters, school life and K - HTP test. With regard to procedure of this study, I carried out art therapy program at the art class for 60 minutes one time a week for 4 months, a total of 17 sessions from Apr. 3, 2007 to July 24, 2007. I made this program with some modification in reference to Arts Therapy Technique (Counpia Remote Training Center, 2005), Group Art Therapy (Choi Seon Nam. Kim Gap Sook. Jeon Jong Kuk, 2007) and the Effects of Peer Group Art Activity on Sociality Skill of Offensive Children (Park Ju Yeon. Lee Byeon In, 2005). For each child, I compared and analyzed the changes in score of K-CBCL children. juvenile behavior evaluation scale, behavior by K-HTP (dynamic house-wood-human figure test) analysis and by school life(KSD) image analysis before and after treatment. I also carried out qualitative analysis on behavioral feature of each child for each session. This study obtained the following conclusions. First, child A showed positive change in comparison before and after art treatment program of score K-CBCL children. juvenile behavior evaluation, K-HTP image test and school life. Since he was so active and wanted to obtain good evaluation from anyone that he frequently had quarrel with friends, spoke against others well and beaten others much but was changed and did not speak against others or beat. Second, child B showed clear difference in comparison before and after art treatment program of score K-CBCL children. juvenile behavior evaluation, but no significant difference in K-HTP image test and school life. However, I observed substantial changes. While he could not speak frankly to others, felt empty in mind and was offensive in personal relations, he was changed, did not have quarrel with others or speak against others that much and improved his relationship with friends or teachers. Third, child C showed positive change in score of K-CBCL children juvenile behavior evaluation after art therapy program. In comparison of K-HTP image test and school life treatment before and after treatment, he still longed for mothers in mind but found rather stable after treatment. Fourth, child D showed positive chance in score of K-CBCL children. juvenile behavior evaluation scale after art therapy program, but almost no changes in K-HTP image test. But he depicted good relationship and socializing with other friends in school life. While he could not carry his point due to social immaturity and felt depressed, he got more confident. This result of study showed that art therapy had positive effects on correction of schoolchildren.

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