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Isolation and Identification of Influenza Viruses from Busan, during 2000-2001 (2000-2001년 부산지역 호흡기 바이러스 발생 양상 비교)

  • 조경순;정명주
    • Korean Journal of Microbiology
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    • v.39 no.2
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    • pp.89-94
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    • 2003
  • Respiratory viruses were isolated from patients with acute respiratory infections in Busan during 2000-2001 and characterized for their antigenic properties. In 2000, 39 out of 43 isolated viruses were identified as influenza viruses and the others were adenoviruses. Among the isolated influenza viruses,23 were type A influenza viruses and 16 were type B influenza viruses. As a result of antigenic characterization, the influenza viruses were determined to A/Sydney/05/97(H3N2)-like, A/Beijing/262/95(H1N1)-like, and B/Harbin07/94-like viruses and serotypes of the isolated adenoviruses were type 1, 2, and 5. In 2001, 56 viruses were isolated and all of the viruses were identified as influenza viruses. They were A/panama/253/99(H3N2)-like and A/Newcaledonia/2007/99(H1Nl)-like viruses when determined by their antigenic properties. The sex distribution of the patients is as follows, 14 males (32.56%),23 females (67.44%) in 2000, and 23 males (41.07%), 33 females (58.93%) in 2001. Occurrence rate was found to be higher in female patients in both years. Age distribution of patients, in 2000, 48.84% of infection occurred in 0 to 1 year old while in 2002, 33.93% occurred among 11-20 year olds. In 2000, occurrence rate was found to be high in January and again in April and various types of viruses were isolated. These results may be useful for vaccine development and establishment of reliable epidemic data.

Study on Design Standards for Nature-like Fishway in Korea (국내 자연형 어도 설계 기준 마련을 위한 고찰 - 자연형 어도 표준 모형안의 문제점과 개선방안을 중심으로 -)

  • Hwang, Sung-Won;Kim, Jin-Oh
    • Journal of Environmental Impact Assessment
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    • v.26 no.3
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    • pp.181-194
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    • 2017
  • While over 90 percent of fishways are made up with concrete material, nature-like fishways are started to be established as a form of small stream using natural materials. Recently, the central government in collaboration with a university has proposed a 'Standard Model of Nature-like Fishway' with a purpose to provide as a national design standard of nature-like fishway. This study aims to criticize the proposed design model of nature-like fishway and to propose improvements by examining other international cases. As a result, we found problems and limitations of the proposed design model due to the lack of research and experience about making nature-like fishway in Korea. Especially, for designing bypass nature-like fishway we found the lack of studies about structure management strategies, establishment of rocks or pebbles inside the stream, and natural materials. In this sense, more studies about nature-like fishway design adaptable to our environment need to be conducted. In addition, since the proposed design standard for nature-like fishway has been created based on the limited examination of existing domestic cases, it is desirable to improve the design standard on the basis of hydrological and biological research so that they could be adapted effectively to regional and local contexts.

Numerical investigation of effects of rotating downdraft on tornado-like-vortex characteristics

  • Cao, Shuyang;Wang, Mengen;Zhu, Jinwei;Cao, Jinxin;Tamura, Tetsuro;Yang, Qingshan
    • Wind and Structures
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    • v.26 no.3
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    • pp.115-128
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    • 2018
  • Appropriate modeling of a tornado-like vortex is a prerequisite when studying the near-ground wind characteristics of a tornado and tornado-induced wind loads on structures. Both Ward- and ISU-type tornado simulators employ guide vanes to induce angular momentum to converge flow in order to generate tornado-like vortices. But in the Ward-type simulator, the guide vanes are mounted near the ground while in the ISU-type they are located at a high position to allow vertical circulation of flow that creates a rotating downdraft to generate a tornado-like vortex. In this study, numerical simulations were performed to reproduce tornado-like vortices using both Ward-type and ISU-type tornado simulators, from which the effects of rotating downdraft on the vortex characteristics were clarified. Particular attention was devoted to the wander of tornado-like vortices, and their dependences on swirl ratio and fetch length were investigated. The present study showed that the dynamic vortex structure depends significantly on the vortex-generating mechanism, although the time-averaged structure remains similar. This feature should be taken into consideration when tornado-like-vortex simulators are utilized to investigate tornado-induced wind forces on structures.

Aroma Characteristics of Raw and Cooked Tenebrio molitor Larvae (Mealworms)

  • Seo, Hojun;Kim, Haeng Ran;Cho, In Hee
    • Food Science of Animal Resources
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    • v.40 no.4
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    • pp.649-658
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    • 2020
  • This study compared aroma compositions and sensory aroma attributes of raw and cooked Tenebrio molitor larvae (mealworms). Main sensory aroma attributes of raw mealworms were strong wet-soil-like, and less-intense oily, shrimp-like and sweet-corn-like. Quantitatively, the major aroma components of raw mealworms were hydrocarbons and aldehydes. As cooking proceeded, sweet-corn-like, roasted, and fried-oil-like sensory attributes were increasingly perceived with steaming, roasting, and frying, respectively. Some pyrazines, pyrrolidines, and carbonyls increased or appeared in roasted and fried mealworms. Partial least squares regression also showed differences in raw and cooked mealworms based on aroma components and their sensory attributes. Unlike raw mealworms, steamed mealworms had a relatively strong sweet-corn-like aroma attribute, which was related to 2,4,6-trimethyl-heptane, 2,4-dimethyl-dodecane, and 3,5-dihydroxy-6-methyl-2,3-dihydropyran-4-one. In comparison, roasted and fried mealworms exhibited roasted, shrimp-like, and fried-oil-like aroma attributes, which were associated with intermediates of the Maillard reaction and lipid oxidation, such as pyrazines, alcohols, and aldehydes. This result during thermal reactions was very similar to those of meat and/or seafood. The use of mealworms as a savory-type flavor enhancer can be expected.

Pedestrian recognition using differential Haar-like feature based on Adaboost algorithm to apply intelligence wheelchair (지능형 휠체어 적용을 위해 Haar-like의 기울기 특징을 이용한 아다부스트 알고리즘 기반의 보행자 인식)

  • Lee, Sang-Hun;Park, Sang-Hee;Lee, Yeung-Hak;Seo, Hee-Don
    • Journal of Biomedical Engineering Research
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    • v.31 no.6
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    • pp.481-486
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    • 2010
  • In this paper, we suggest an advanced algorithm, to recognize pedestrian/non-pedestrian using differential haar-like feature, which applies Adaboost algorithm to make a strong classification from weak classifications. First, we extract two feature vectors: horizontal haar-like feature and vertical haar-like feature. For the next, we calculate the proposed feature vector using differential haar-like method. And then, a strong classification needs to be obtained from weak classifications for composite recognition method using the differential area of horizontal and vertical haar-like. In the proposed method, we use one feature vector and one strong classification for the first stage of recognition. Based on our experiment, the proposed algorithm shows higher recognition rate compared to the traditional method for the pedestrian and non-pedestrian.

Characterization of the molecular features and expression patterns of two serine proteases in Hermetia illucens (Diptera: Stratiomyidae) larvae

  • Kim, Won-Tae;Bae, Sung-Woo;Kim, A-Young;Park, Kwan-Ho;Lee, Sang-Beom;Choi, Young-Cheol;Han, Sang-Mi;Park, Young-Han;Koh, Young-Ho
    • BMB Reports
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    • v.44 no.6
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    • pp.387-392
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    • 2011
  • To investigate the molecular scavenging capabilities of the larvae of Hermetia illucens, two serine proteases (SPs) were cloned and characterized. Multiple sequence alignments and phylogenetic tree analysis of the deduced amino acid sequences of Hi-SP1 and Hi-SP2 were suggested that Hi-SP1 may be a chymotrypsin- and Hi-SP2 may be a trypsin-like protease. Hi-SP1 and Hi-SP2 3-D homology models revealed that a catalytic triad, three disulfide bonds, and a substrate-binding pocket were highly conserved, as would be expected of a SP. E. coli expressed Hi-SP1 and Hi-SP2 showed chymotrypsin or trypsin activities, respectively. Hi-SP2 mRNAs were consistently expressed during larval development. In contrast, the expression of Hi-SP1 mRNA fluctuated between feeding and molting stages and disappeared at the pupal stages. These expression pattern differences suggest that Hi-SP1 may be a larval specific chymotrypsin-like protease involved with food digestion, while Hi-SP2 may be a trypsin-like protease with diverse functions at different stages.

Design of High-performance Pedestrian and Vehicle Detection Circuit using Haar-like Features (Haar-like 특징을 이용한 고성능 보행자 및 차량 인식 회로 설계)

  • Kim, Soo-Jin;Park, Sang-Kyun;Lee, Seon-Young;Cho, Kyeong-Soon
    • The KIPS Transactions:PartA
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    • v.19A no.4
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    • pp.175-180
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    • 2012
  • This paper describes the design of high-performance pedestrian and vehicle detection circuit using the Haar-like features. The proposed circuit uses a sliding window for every image frame in order to extract Haar-like features and to detect pedestrians and vehicles. A total of 200 Haar-like features per sliding window is extracted from Haar-like feature extraction circuit and the extracted features are provided to AdaBoost classifier circuit. In order to increase the processing speed, the proposed circuit adopts the parallel architecture and it can process two sliding windows at the same time. We described the proposed high-performance pedestrian and vehicle detection circuit using Verilog HDL and synthesized the gate-level circuit using the 130nm standard cell library. The synthesized circuit consists of 1,388,260 gates and its maximum operating frequency is 203MHz. Since the proposed circuit processes about 47.8 $640{\times}480$ image frames per second, it can be used to provide the real-time detection of pedestrians and vehicles.

Biosynthesis of a Biological Active Single Chain Equine Chorionic Gonado-tropin

  • Min, Kwan-Sik
    • Journal of Life Science
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    • v.11 no.2
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    • pp.103-107
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    • 2001
  • The equine chorionic gonadotropin (eCG) subunits $\alpha$ and ${\beta}$ are transcribed from different genes and associate noncovalently to form the bioactive eCG heterodimer. Dimerization is rate limiting for eCG secretion, and dissociation leads to hormone inactivation. The correct conformation of the heterodimer is alto important for efficient secretion, hormone-specific post-translational modifications, receptor binding and signal transduction. To determine whether ${\alpha}$ and ${\beta}$ subunits can be synthesized as a single polypeptide chain (tethered-eCG) and also display biological activity, the tethered-eCG molecule by fusing the carboxyl terminus of the eCG ${\beta}$-subunit to the amino terminus of the af-subunit was construe-ted and transfected into chinese hamster ovary (CHO-Kl) cells. LH- and FSH-like activities were assayed in terms of testosterone production and aromatase activity in primary cultured rat Leydig cells and granulosa cells, respectively. The tethered-eCG was efficiently secreted and showed similar LH-like activity to the dimeric eCG ${\alpha}$/${\beta}$ and native eCG. FSH-like activity of the tethered-eCG was also shown similarly in comparison with the native and wild type eCG ${\alpha}$/${\beta}$. Our data for the first time suggest that the tethered-eCG can be expressed efficiently and the produced product by the CHO-K1 cells is fully LH- and FSH-like activities in rat in vitro bioassay system. Our results also suggest that this molecular can imply particular models of FSH-like activity not LH-like activity in the eCG. Taken together, these data indicate that the constructs of tethered molecule will be useful in the study of mutants that affect subunit association and/or secretion.

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Effect that Gagman Advertisement Model Use by Product Involvement gets in Brand Preference, Purchase Intention (제품 관여도에 따른 개그맨 광고 모델 사용이 브랜드 선호도, 구매 의도에 미치는 영향: 인쇄 광고를 중심으로)

  • Lee, Kwang-Sook
    • Journal of the Korean Graphic Arts Communication Society
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    • v.30 no.3
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    • pp.65-75
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    • 2012
  • Appearing gagman on various ads are presently experienced in domestic market both high involvement and low involvement products ads. Therefore, this research attempts to analyze the effectiveness of using gagman ad model in terms of building brand preference and purchasing intention among target audience. Results of this study proposed the basement of practical use when gagman is selected as a ad model in the field. For testing hypotheses attractiveness, reliability, like/dislike as dependent variables and as independent variable purchasing intention were selected. The result of analysis shows that for high involvement product reliability influenced on brand like/dislike and attractiveness of gagman model is effective to enhance purchasing intention. For low involvement product only like/dislike was significant. This can be interpreted gagman ad model is useful for building brand like/dislike and purchasing intention of high involvement product when gagman has reliability and attractiveness respectively, while for low involvement product, like and dislike is the only variable to be considered in choosing gagman ad model.

Development of Worm-like Polymeric Drug Carriers with Multiple Ligands for Targeting Heterogeneous Breast Cancer Cells

  • Lee, A-Hyeong;Oh, Kyung-Taek;Baik, Hye-Jung;Lee, Bo-Reum;Oh, Young-Taik;Lee, Don-Haeng;Lee, Eun-Seong
    • Bulletin of the Korean Chemical Society
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    • v.31 no.8
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    • pp.2265-2271
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    • 2010
  • In this study, wormorm-like polymeric micelles were construted from poly(L-lactic acid)-b-poly(ethyelen glycol) (PLLA-b-PEG) block copolymers via worm-like (or cylindrical) self- assembly that consisted of a relatively long PLLA block ($M_n$ 7K Daltons) at the core and a relatively short PEG block ($M_n$ 2K Daltons) as the shell. Several cancer-targeting moieties (such as folate, cobalamin, and cyclic arginine-glycine-aspartic (RGD) peptide) were chemically coupled with the succinylated or maleimided PEG block of PLLA-b-PEG to act as a cancer cell-specific targeting ligand for breast cancer. The worm-like micelles with muplite cancer cell-specific ligands proved to be successful in recognizing different breast cancer cells at once. This has the potential to aid in cancer-specific drug delivery and to be used as an effective treatment for breast cancer.