• Proceedings of the Korean Society of Applied Pharmacology
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• 2001.11a
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• pp.102-102
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• 2001

Taurine (2-ethaneaminosulfonic acid) is one of the major intracellular ${\beta}$ -amino acids in mammals and is required for a number of biological processes including membrane stabilization, osmoregulation, antioxidation, detoxification, modulation of calcium flux and neurornodulation. The taurine transporter (TAUT) which contains 12 hydrophobic membrane-spanning domains has been cloned from dog kidney, rat brain, mouse brain, human thyroid, placenta and retina. In this study, The TAUT cDNA from the human intestinal epithelial cell, HT-29 was cloned and sequenced. Reverse-transcription polymerase chain reaction (RT-PCR) was performed to amplify partial cDNA encoding human intestinal TAUT. The coding region of the PCR product was 732 bp long. The primers were designed to encode highly conserved amino acid sequences near the transmembrane domains III (IPYFIFLF) and Ⅵ (KYKYNSYR) both in human and mouse. The TAUT cDNA amplified was ligated into the pGEX 4T-1 expression vector. The resulting sequence of human intestinal TAUT cDNA (Accession number of NCBI Genebank is AF346763) was identical to the sequences of the TAUTs previously determined in the human placenta and retina except 3 base pairs from that of the reported human thyroid. TAUT specific antibodies were generated to use them as biological tools in the studies of the biological role of TAUT. Peptides of 149-162 amino acid residue (14 amino acids) of the TAUT were synthesized. The synthetic peptide used in this study was LFQSFQKELPWAHC. This region was chosen not only to avoid putative glycosylation sites but also to exclude regions of known homology with GABA transporters in the extracellular hydrophilic domains. The synthetic peptide, TAUT-1 was conjugated with carrier protein, kehole lympet hemocyanin (KLH) to use as an antigen. When used for immunization on a rabbit to produce polyclonal antiserum, the conjugates elicited high -titered specific anti-TAUT-1 antibodies, which reacted well with the ovalbumin (OVA) conjugated peptides in ELISA. The KLH-conjugated peptide was also used as immunizing antigen in BALB/c mice to produce TAUT specific monoclonal antibodies. From the culture supernatant of the hybridoma, the specificity of anti-TAUT-1 monoclonal antibodies was confirmed by ELISA. Further applications of more tools in TAUT expression analysis will be performed such as western blotting and flow cytometry.

• Information Systems Review
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• v.9 no.3
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• pp.99-132
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• 2007

As customer management rises by important issue in electronic commerce, virtue study about CRM have proceeded much. However, because existent researches were positive researches of most statistical base, There are some limitation that does not show dynamic change with CRM flow by flowing of time, and can not forecast propriety and future result about CRM strategy. Therefore, in order to overcome existent limitation on these CRM study, this study designed dynamic model which draws factors that compose CRM strategy of on-line shopping mall, and do based on technique in system dynamics so that can analyze dynamic change between these factors. Concretely, atomized customer focuses in the on-line shopping mall and does based on Permission marketing theory, and applied CRM of different level to atomized customers and know change of customer satisfaction measurement and discomfort degree accordingly. According to the result of Simulation practice, situation that achieve CRM strategy of different level by atomize customer more increase the customer satisfaction than situation that is not so. Dynamic pattern that presented in this study is expected that can verify validity about CRM achievement strategy of different level at each CRM point of contact & how Internet enterprise including on-line shopping mall is establishing CRM strategy reasonably.

• Economic and Environmental Geology
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• v.39 no.5 s.180
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• pp.607-613
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• 2006

For the reasonable use of low grade-shallow geothermal energy by Standing Column Well(SCW) system, the basic requirements are depth-wise increase of earth temperature like $2^{\circ}C$ per every 100m depth, sufficient amount of groundwater production being about 10 to 30% of the design flow rate of GSHP with good water quality and moderate temperature, and non-collapsing of borehole wall during reinjection of circulating water into the SCW. A closed loop type-vertical ground heat exchanger(GHEX) with $100{\sim}150m$ deep can supply geothermal energy of 2 to 3 RT but a SCW with $400{\sim}500m$ deep can provide $30{\sim}40RT$ being equivalent to 10 to 15 numbers of GHEX as well requires smaller space. Being considered as an alternative of vertical GHEX, many numbers of SCW have been widely constructed in whole country without any account for site specific hydrogeologic and geothermal characteristics. When those are designed and constructed under the base of insufficient knowledges of hydrgeothermal properties of the relevant specific site as our current situations, a bad reputation will be created and it will hamper a rational utilization of geothermal energy using SCW in the near future. This paper is prepared for providing a guideline of SCW design comportable to our hydrogeothermal system.

• Journal of the Korean Wood Science and Technology
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• v.33 no.6 s.134
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• pp.63-70
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• 2005

The purpose of this study was to investigate how to modify the physical properties of cellulose after thermal treatment. Cellulose was treated between $225^{\circ}C$ and $325^{\circ}C$ for 3 hrs under air flow, and then the thermally treated cellulose was measured to specific surface area, constitute elements, consumption ofacid and base, as well as the adsorption capacity of ethylamine vapor. The higher was the treating temperature from $225^{\circ}C$ to $325^{\circ}C$, the lower was the total yield of cellulose. Elemental analysis revealed that carbon content in thermally treated cellulose was gradually increased in proportion to temperature increment. The amount of acidic functional groups tended to increase up to $300^{\circ}C$, after then to be lowered slightly. In principle, no alkaline functional groups were found in thermally treated cellulose. In case of treatment with $325^{\circ}C$, only a few amount of alkaline functional groups were detectable. Specific surface area of thermally treated cellulose are determined to $1.9m^2/g$, which value can become higher when the treated temperature rises. The thermally treated cellulose at $275^{\circ}C$ shows the highest adsorption capacity of ethylamine at $40^{\circ}C$ for 4 hrs. Solubility of those two celluloses with WPG (Weight Percent Gain) value of 113% and 108%, respectively, was determined to almost 100%. X-ray diffractogram of thermally treated cellulose suggested that the crystalline structure of cellulose began to be destroyed at the temperature of $275^{\circ}C$. As a conclusion, changes of such a physical properties make it possible to weaken inter and/or intra hydrogen bond in crystal region of cellulose macromolecules. When thermally treated cellulose adsorbs ethylamine, it turns to be well soluble to water.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.12 no.2
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• pp.105-115
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• 2017

Innovative cluster theory promotes cluster growth as the tacit knowledge and know-how approach becomes easier through industry-academia cooperation. Industry-academia cooperation is an innovation network policy that supports joint research between industry and academia. In this respect, The Flow of recent government policy is activating I-U support office in university & research institute for enable I-U Cooperation ecosystem. Then SMB Administration was first performed "research village support program", to support SMEs in industry-university cooperative research capabilities by integrating the research, development and commercializatin of the university or research institution with excellent research base in 2013. However, I-U Cooperation R&D must be based the link strategy of Localization in order to be better composition at research village. In the case of research villages where specialized discovery strategies are well reflected, integration of similar companies in specialized fields will naturally create clusters and create synergy of research. This study searching and summarizing through a recent Hanbat National University research village. Finally, we propose the implications of government policy.

• Journal of Korea Water Resources Association
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• v.46 no.9
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• pp.921-932
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• 2013

Runoff data availability is a substantial factor for precise flood control such as flood frequency or flood forecasting. However, runoff depths and/or peak discharges for small watersheds are rarely measured which are necessary components for hydrological analysis. To compensate for this discrepancy, a lumped concept such as a Storage Function Method (SFM) was applied for the partitioned Choongju Dam Watershed in Korea. This area was divided into 22 small watersheds for measuring the capability of spatial extension of runoff data. The chosen total number of flood events for searching parameters of SFM was 21 from 1991 to 2009. The parameters for 22 small watersheds consist of physical property based (storage coefficient: k, storage exponent: p, lag time: $T_l$) and flood event based parameters (primary runoff ratio: $f_1$, saturated rainfall: $R_{sa}$). Saturated rainfall and base flow from event based parameters were explored with respect to inflow at Choongju Dam while other parameters for each small watershed were fixed. When inflow of Choongju Dam was optimized, Youngchoon and Panwoon stations obtained average of Nash-Sutcliffe Efficiency (NSE) were 0.67 and 0.52, respectively, which are in the satisfaction condition (NSE > 0.5) for model evaluation. This result is showing the possibility of spatial data extension using a lumped concept model.

• Journal of Korea Water Resources Association
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• v.40 no.12
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• pp.1007-1014
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• 2007

Recently, existing river improvement methods for flood control purpose have changed into nature friendly river improvement methods and the efforts to improve the river environment including river restoration have been made, and close-to-nature river improvement and nature friendly river restoration are actively conducted all over the country. In the present situation where various revetment methods are used after the introduction of the concept of close-to-nature river improvement, the environmental characteristics of rivers need to be considered to apply more suitable revetment methods. Therefore, as a precedent study for the development of revetment evaluation techniques and methods for close-to-nature river improvement, the present study suggested evaluation techniques using detailed survey items through field survey. Evaluation items of hydraulic stability consist of mode of streamline, stream bed gradient, flow velocity and tractive force ratio and those of environmental efficiency consist of revetment of vegetation, state of river water, land use of the terrace land on the river, vegetation and materials of the terrace land on the river. Each item was graded with the point 1 through 5. Hydraulic stability and environmental efficiency was evaluated by the points which were averaged in each items. As the result of the application of the proposed evaluation technique, it was found that a number of existing revetments excessively focus on hydraulic stability with little consideration about environmental term. It is expected that the proposed technique in the present study can be used as a base for providing guidelines to construct the design and construction of revetments in the future.

• Journal of KIISE:Software and Applications
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• v.36 no.1
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• pp.70-81
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• 2009

Ontology is an explicit specification of concepts and relationships between concepts in an interest domain. As considered as one of typical knowledge representation methods, ontology is applied to various studies such as information extraction, information integration, information sharing, or knowledge management. In IT based industries, ontology is applied to research on information integration and sharing in order to enhance interoperability between enterprises. In supply chains or logistics, several enterprises participate as business partners to plan movements of goods, and control goods and logistics flows. A number of researches on information integration and sharing for the effective and efficient management of logistics or supply chains have been addressed. In this paper, we address an ontology as a knowledge-base for semantic-based integration of logistics information distributed in the logistics flow. Especially, we focus on developing an ontology that enables to represent and translate semantic meaning of EPC data in the EPC Network applied logistics. We present a scenario for tracing products in logistics in order to show the value of our ontology.

• Journal of Korean Society of Disaster and Security
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• v.13 no.2
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• pp.53-63
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• 2020

In this study, the two-dimensional flow analysis model Hydro_AS-2D model was used to simulate the situation of flooding in Seongsangu and Uichang-gu in Changwon in the event of rising sea levels and extreme flooding, and the results were expressed on three-dimensional topography and the optimal evacuation path was derived using BIM technology. Climate change significantly affects two factors in terms of flood damage: rising sea levels and increasing extreme rainfall ideas. The rise in sea level itself can not only have the effect of flooding coastal areas and causing flooding, but it also raises the base flood level of the stream, causing the rise of the flood level throughout the stream. In this study, the rise of sea level by climate change, the rise of sea level by storm tidal wave by typhoon, and the extreme rainfall by typhoon were set as simulated conditions. The three-dimensional spatial information of the entire basin was constructed using the information of topographical space in Changwon and the information of the river crossing in the basic plan for river refurbishment. Using BIM technology, the target area was constructed as a three-dimensional urban information model that had information such as the building's height and location of the shelter on top of the three-dimensional topographical information, and the results of the numerical model were expressed on this model and used for analysis for evacuation planning. In the event of flooding, the escape route is determined by an algorithm that sets the path to the shelter according to changes in the inundation range over time, and the set path is expressed on intuitive three-dimensional spatial information and provided to the user.

• Journal of Korean Society of Rural Planning
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• v.6 no.1 s.11
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• pp.19-28
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• 2000

Now in order to overcome the weakest points of the rural areas of the city of Kimje and, transform them into rural cultural villages which have local governing systems suitable to new localization age and activate this plan, we selected Daesan Village as a model village which had shown a lot of potentials in the basic research and studied it dividing it into the former part and the latter part. We studied Daesan village in the former part focusing on state analysis and basic ideas and in the latter part focusing on master plan and detail planning. We can summarize the conclusion like the followings. 1. Daesan Village located 8 kilometer away from the downtown Kimje and the city of Iksan respectably has comparatively good environment of good sunny place as an open field whose surrounding configuration of the ground consists of farming lands and low hills in front and rear. It has 38 farming households in all. 2. Human environment(인문환경); the village road whose width is about 4 meters is forming a flow system forking off into three. There is a route bus which operates three times a day even into the inside of the village. The main sources of revenue are vegetables in facilities, fruits and floriculture. Their average revenue is about 10.5 million won. 3. Here in DaeSan Village a legend dealing with Teasan literally meaning a big mountain consist of th village's tradition and you can see the tombs of a very faithful son and Anwi an army general in the age of the Japanese Invasion of Korea of 1592 to 1598 inside the village. 4. 85 out of the eitire population 141 whose age are over 20 showed very positive attitudes in a questionnaire about, making the village a cultural one and its development. 5. The basic of planned ideas is to increase the revenue of the farming household by making the village a professional farming one which has a state-of the-art production facility and agricultural technique. It is to make the village the one where people can enjoy the sense of the rural life and the farmer can enjoy their lives through consumptive and consistant leisure and resting activities. 6. We are planning to make entrance space, life space, rest and sport space, and cultural space considering the characteristics of the village and the demand of the resident. We are also planning to make tile entire city of Kimje an information transmitting base in short and long term perspectives. 7. DaeSan Village was planned as a place where tradition and the future exist together. On the basis of this concept we planned future programs for Daesan Village and in the latter part of the study master plans and detail plans will be continued.the regional agricultural condition. The development permissions were only during the period of restricted to use ($1979.12{\sim}1993.11$). We propose that the authority of development permission should be given to the local autonomy government, because the local government has the knowledge of its individual agricultural conditions.