• Fisheries and Aquatic Sciences
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• 제24권9호
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• pp.297-310
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• 2021

The rockworm gut flora was identified at each growth stage to elucidate the composition of the bacterial community. The source material was Marphysa sanguinea fed regular feed and fed biofloc at the adult stage in parallel. The systematic bacterial community composition was determined based on the next-generation sequencing method, and alpha diversity and beta diversity were conducted to access the species diversity within and between the bacterial communities, respectively. The composition of the gut flora changed considerably as the rockworms developed. The shift in the gut flora was confirmed at the phylum, family, and genera level of the bacterial communities. The Vibrio species associated with high rockworm mortality occupied 7.7% of the gut flora at the larval stage; however, they disappeared in the healthy adult gut. Moreover, different gut flora was observed between adults fed regular feed and those fed biofloc. Specifically in the biofloc-fed adult gut, several immune relevant and water-purifying bacteria were detected. The biofloc-fed adult gut flora could decompose and mineralize organic sediment, and thus be effectively utilized for integrated multitrophic aquaculture. The Venn diagram revealed that only two bacterial species were shared throughout all growth stages, and the biofloc-fed adults exhibited the highest diversity within the bacterial community.

• Journal of Microbiology
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• 제40권2호
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• pp.104-108
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• 2002

The hazelnut leaf holer (Anoplus roboris Sufr,, Coleoptera: Curculionidae) is a devastating pest of hazelnut and oak trees. It causes approximately 20-30% economic damage to hazelnut production per year in Turkey. In the present study, in order to fond a more effective and safe biological control agent against A. roboris, we investigated the bacterial flora of the hazelnut leave holer, and tested them for insecticidal effects on it. According to morphological, physiological and biochemical tests bacterial flora were identified as Bacillus circulans (Ar1), Bacillus polymyxa (Ar2), Enterobacter sp. (Ar3) and Bacillus sphaelicus (Ar4). Insecticidal effects of bacterial isolates were performed on adult A. roboris. The highest insecticidal effect determined was 67% by B. sphaericus within eight days. The insecticidal effects of the other isolates (Ar1, Ar2 and Ar3) were determined as 33%, 47% and 47% within the same period, respectively.

• Journal of Microbiology and Biotechnology
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• 제17권1호
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• pp.146-153
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• 2007

A mathematical competition model between normal flora and an invading pathogen was devised to allow analysis of bacterial infections in a host. The normal flora includes the various microorganisms that live on or within the host and act as a primary human immune system. Despite the important role of the normal flora, no mathematical study has been undertaken on models of the interaction between it and invading pathogens against a background of antibiotic treatment. To quantify key elements of bacterial behavior in a host, pairs of nonlinear differential equations were used to describe three categories of human health conditions, namely, healthy, latent infection, and active infection. In addition, a cutoff value was proposed to represent the minimum population level required for survival. The recovery of normal flora after antibiotic treatment was also included in the simulation because of its relation to human health recovery. The significance of each simulation parameter for the bacterial growth model was investigated. The devised simulation showed that bacterial proliferation rate, carrying capacity, initial population levels, and competition intensity have a significant effect on bacterial behavior. Consequently, a model was established to describe competition between normal flora and an infiltrating pathogen. Unlike other population models, the recovery process described by the devised model can describe the human health recovery mechanism.

• 대한수의학회지
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• 제54권4호
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• pp.219-224
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• 2014

Uterine sterilization is important for improving fertility in cattle. This study compared bacterial flora in the uterus between healthy and repeat breeder cows (RBCs). The uterine flushing of six heifers, 13 healthy HanWoo cows and eight RBCs (HanWoo) were sampled, and 15 frozen semen samples were selected. Overall, 35 bacteria were identified from in HanWoo uterine flushing and semen. The bacterial genera identified from HanWoo uterine flushing were Alloiococcus, Bacillus, Enterobacter, Enterococcus, Erysipelothrix, Gardnerella, Granulicatella, Kocuria, Pantoea, Pasteurella, Rothia, Serratia, Sphingomonas, Staphylococcus, Stenotrophomonas and Streptococcus. The bacterial genera identified from HanWoo semen were Bacillus, Escherichia, Kocuria, Oligella, Pseudomonas, Serratia, Sphingomonas, Staphylococcus, Stenotrophomonas and Streptococcus. The prevalence and presence of the identified bacteria between healthy cows and RBCs differed significantly. Further studies are needed to determine the role of these bacteria in the uterus of HanWoo cattle with reproductive disorder.

• 미생물학회지
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• 제12권1호
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• pp.1-14
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• 1974

The strain of Peptostreptococcus sp. S99 used in this study was isolated from the serous discharge of omphalitis of a 6 days old icteric male infant at the Clinical Laboratory of Microbiology, Seoul National University Hospital on June 9, 1973. The purpose of this study is to clarify the commensalism between Peptostreptococcus sp. and the human bacterial flora isolated from clinical specimens with special references to pH.

• 한국미생물·생명공학회지
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• 제7권3호
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• pp.175-179
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• 1979

참굴의 성수기인 1978년 12월, 1979년 1월 및 2월에 걸쳐 주산지인 고성만과 돌산의 참굴과 그 참굴이 서식한 해수 및 이토 등을 시료로하여 세균의 오염도와 그 동정을 한 결과 다음과 같이 요약된다. 1. 생균수의 범위는 참굴, 이토, 해수에서 각각 $10^4$~$10^{6}$, $10^{5}$ ~$10^{6}$, $10^3$~$10^4$ 이었다. 2. 분리균 382균주 중에서 Vibrio속이 45.3%로 우점종을 이루었고, Pseudomonas속이 16.7%, Moraxella속이 11.5%, Flavobacterium-cytophaga속이 8.9%로 이들 4균속은 82.4% 나되어 참굴 및 그 서식한 해수 및 이토중에 중요한 세균 Flora 로 나타났다. 3. Vibrio속으로 동정된 173균주 중 참굴에서 56.3%, 이토에서 54.0%, 해수에서 25.8%로 해수 중의 분포도가 현저히 낮게 검출되었다. 4. Pseudomonas속 가운데에서 Pseudomonas I 균형은 해수에만 검출되었고 Pseudomonas III/IV 균형도 해수에서 많이 출현하였으며 그 외 균속은 큰 변동이 보이지 않았다.

• 한국어병학회지
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• 제13권1호
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• pp.67-73
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• 2000

군산내만 해역의 해양생태계에 관한 기초자료를 얻기 위하여 금강 하구둑부터 외항까지 5개의 정점을 선정하여 종속영양 세균상의 분포를 조사하였다. 조사지역으로부터 123개의 집락을 분리-동정한 결과 Vibrio spp.(44주, 35.7%), 및 Pseudomonas spp.(42주, 34.1%), 균종이 비교적 높게 출현하였고 Aeromonas spp.(11주, 8.9%), Moraxella spp.(9주, 7.3%), Escherichia coli.(6주, 4.8%), Bordetella spp.(3주, 2.4%), Alkaligenesis spp.(3주, 2.4%), Flavobacterium spp.(2주, 1.6%), and Staphylococcus spp.(3주, 2.4%)등은 낮은 출현율을 나타냈다. 한편, 각 총 정점의 생균수는 $5{\times}10^3\sim1{\times}10^5$ CFU/ml이었으며, 생활 하수가 많이 유입되는 지역(정점 2.3)이 강 하구 및 외항지역보다 높은 검출율을 보였다. 이러한 결과는 도시의 생활 오.하수 및 산업 부산물 등의 유입으로 내만의 수질 악화가 지속적으로 진행되고 있는 것으로 추정된다. 따라서 양식사업에 있어서 문제시 되고 있는 어류의 세균성 질병의 발생이 증가될 것으로 사료되어진다.

• 수산해양교육연구
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• 제28권6호
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• pp.1828-1833
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• 2016

The purpose of this research was to investigate the bacterial flora of healthy olive flounder larvae (Paralichthys olivaceus) and live feeds (Rotifer spp. and Artemia spp.). The total bacteria counts were $9.2{\times}10^7$ and $1.2{\times}10^{10}cfu/g$ and Vibrio sp.(82.8%) was dominant in rotifers. The total bacteria counts were $3.8{\times}10^6$ and $9.2{\times}10^6cfu/g$ and Vibrio sp.(73.3%) was dominant in artemia. In olive flounder larvae, the total bacteria counts were $1.4{\times}10^6{\sim}8.3{\times}10^7cfu/g$ and V. harveyi (38.5%) was dominant. It might be potential marker of disease outbreak in olive flounder larvae.

• 한국토양비료학회지
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• 제18권2호
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• pp.221-226
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• 1985

밭토양(土壤)에서 살충제(殺蟲劑) Hexachlorocyclohexane(HCH) 및 살균제(殺菌劑) Tetrachloroisophthalonitrile(TPN)의 연용(連用)이 토양세균(土壤細菌) flora의 구성(構成)을 어떻게 변화(變化)시키는가를 검토(檢討)하기 위하여 HCH 및 TPN을 년(年)1회(回) 2년간(年間) 연용(連用)한 각실험구(各實驗區) 그리고 대조구(對照區)로서 농약(農藥) 무처리구(無處理區)로부터 단리(單離)한 세균세포(細菌細胞)의 현미경적(顯微鏡的) 관찰(觀察)과 생리적(生理的) 시험(試驗)을 하였다. 그리고 이 형태적(形態的) 및 생리적(生理的) 결과(結果)에 기초(基礎)한 Cowan & Steel의 제(弟)1차(次) 감별기준(鑑別基準)에 따라 이들 단리균주(單離菌株)를 Grouping하여 다음과 같은 결과(結果)를 얻었다. 1. 희석평판법(稀釋平板法)에 의한 알부민 한천배지상(寒天培地上)에 형성(形成)된 micro-colony를 육즙한천배지(肉汁寒天培地)에 이식배양(移植培養)하여 순수분리(純粹分離)하는 과정(過程)에서 많은 세균(細菌)이 사멸(死滅)하였는데 대조구(對照區)에서 30%, HCH구(區)에서 44%, TPN구(區)에서 51%의 사멸률(死滅率)을 각각 보였다. 이는 이들 농약(農藥)의 연용(連用)에 의하여 사멸(死滅)하기 쉬운 즉(卽) 영양요구성(營養要求性)이 복잡(複雜)한 세균군(細菌群)이 토양(土壤)중에 집적(集績)되었음을 시사(示唆)한다. 2. 전단리균주(全單離菌株)에 대한 그람음성세균(陰性細菌)은 대조구(對照區)에서 37%, HCH구(區)에서 37%, TPN구(區)에서 75%를 각각 나타내어 TPN은 그람음성세균군(陰性細菌群)을 토양(土壤)중에 우점(優占)시켰다. 3. 각실험구(各實驗區)로부터 단리(單離)된 균주(菌株)에 대한 현미경적(顯微鏡的) 관찰(觀察)과 생리적(生理的) 시험결과(試驗結果)로부터 HCH와 TPN은 서로 다른 pattern으로 토양세균(土壤細菌) flora의 구성(構成)을 변화(變化)시켰다. 4. 전단리균주(全單離菌株)에 대한 포자형성세균(胞子形成細菌)은 대조구(對照區)에서 77%, HCH구(區)에서 40%, TPN구(區)에서 0%를 각각 나타내어 농약처리구(農藥處理區)에서 포여형성세균군(胞予形成細菌群)이 저하(低下)되었고, 특(特)히 TPN구(區)에서는 포자형성균(胞子形成菌)이 전(全)혀 분리(分離)되지 않았다. 5. HCH 혹은 TPN의 살포(撒布)에 의하여 Group I(Bacillus)에 유별(類別)되는 포자형성세균군(胞子形成細菌群)이 감소(減少)된 반면(反面)에 그람음성(陰性)의 포자비형성세균군(胞子非形成細菌群)이 증가(增加)되어, HCH 및 TPN은 토양세균(土壤細菌) flora의 구성(構成)을 현저(顯著)하게 변화(變化)시켰다.

• International Journal of Oral Biology
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• 제39권3호
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• pp.137-143
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• 2014

Dental professionals are repeatedly exposed to many microorganisms present in both blood and saliva. Thus, dental professionals are at a greater risk of acquiring and spreading infections, and the implementation of infections control guidelines is necessary. Cellular phones have become a necessary device for communicating in hospitals. Cellular phones contaminated with bacteria may serve as a fomite in the transmission of pathogens by the hands of medical personnel. Nevertheless, studies about rate and levels of bacterial contamination of cellular phones have been extremely limited with regards to dental personnel. The purpose of this study was to identify bacterial flora on the cellular phones of dentists by a molecular biological method using the 16S rRNA cloning and sequencing method. We acquired total 200 clones from dentists' cell phones and identified the bacterial species. Pseudomonas (34.6%), Lactobacillus (18.5%), Azomonas (11.5%), and Janthinobacterium (6%) were the dominant genera on dentists' cell phones. The oral bacteria identified were Anaerococcus lactolyticus, Gibbsiella dentisursi, Lactobacills leiae, Streptococcus mitis, Streptococcus oligofermentans, and Streptococcus sanguinis. Pathogenic bacteria and opportunistic pathogens such as Carnobacterium funditum, Raoultella planticola, Shigella flexneri, Lactobacillus iners, Staphylococcus aureus, and Staphylococcus epidermidis were also identified.