• 미생물학회지
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• 제54권4호
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• pp.398-409
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• 2018

본 연구에서는 된장으로부터 바이오제닉 아민 생성 바실러스균과 박테리오신 생산유산균은 16S rRNA 염기서열 분석을 통해 동정하였고, 이들 균주들의 혼합 배양으로 제조한 청국장의 이화학적 및 미생물학적 특성을 조사하였다. 총10종의 시료로부터 바이오제닉 아민을 생성하는 바실러스균은 Bacillus licheniformis DB102, B. subtilis DB203, B. stearothermophilus DB206, B. pumilus DB209, B. subtilis DB310, B. coagulans DB311, B. cereus DB313, B. amyloliquefaciens DB714, B. amyloliquefaciens DB915, B. licheniformis DB917, B. cereus DB1019, B. subtilis DB1020, B. megaterium DB1022로 동정되었다. 바이오제닉 아민 생성 바실러스균에 대해 항균 활성을 나타낸 박테리오신 생산균은 Lactobacillus plantarum DLA 205, L. brevis DLA501, L. fermentum DLA509, L. acidophilus DLA703 및 Enterococcus faecalis DLA804로 확인되었다. 유산균의 박테리오신은 농도의존적으로 바이오제닉 아민 생성균의 생균수 및 아민 생성능을 유의하게 감소시켰다. 따라서 유해 아민 생성균에 대한 항균 활성을 나타낸 유산균의 증식에 따라 유산균과 바실러스균을 혼합 배양하여 제조한 청국장의 pH, 암모니아태 질소 함량 및 바이오제닉 아민 함량은 대조구에 비해 유의하게 감소되었다.

• Applied Biological Chemistry
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• 제49권3호
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• pp.175-179
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• 2006

동일비료를 장기연용한 토양에서 벼를 단작으로 재배하여 비료의 시용에 따른 벼 품종간 근권 세균 및 방선균의 밀도와 우점균을 조사하였다. 비료는 3요소(NPK)를 기본으로 하여 무질소(PK), 무인산(NK) 무칼리(NP) 및 NPK+퇴비를 처리하였다. 벼의 근권 미생물 밀도는 품종에 따라서는 큰 차이를 보이지 않았고 시용비료에 의해 약간의 차이를 보였는데 세균은 감소하고 방선균은 증가하는 경향을 나타내었다. 근권 미생물의 분포상에는 유기물의 영향이 뚜렷하여 방선균의 경우 NPK+퇴비구 처리구에서 가장 높은 균 밀도를 나타냈다. 배양가능한 호기성 세균에서는 Bacillus속의 B. megaterium, B. mycoides, B. licheniformis, B. subtilis 등이, 방선균에서는 Streptomyces속의 S. spororaveus, S. canus, S. tauricus, S. galbus 등과 Micromonospora속이 우점하여 분포하였다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제8권1호
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• pp.37-40
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• 2003

The antibacterial action of violet pigment, a mixture of violacein and deoxyviolacein, isolated from phychrotrophic bacterium RT102 strain was examined, and the operational conditions for the effective production of violet pigment were studied. The antibacterial activity of the violet pigment was confirmed for several bacteria such as Bacillus licheniformis, Bacillus subtilis, Bacillus megaterium, Staphylococcus aureus, and Pseudomonas aeruginosa, and the high concentration of violet pigment, above about 15mg/L, caused not only growth inhibition but also death of cells. The growth properties of RT102 strain were clarified under various incubation conditions such as pH, temperature, and dissolved oxygen concentration. The maximum violet pigment concentration, i.e. 3.7 g/L, and the maximum productivity of violet pigment, i.e. 0.12 g .L$\^$-1/H$\^$-1/, were obtained in a batch culture of pH 6, 20$^{\circ}C$, and 1 mg/L of dissolved oxygen concentration.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2007년도 추계종합학술대회
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• pp.989-992
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• 2007

Twenty bacterial strains were isolated and identified from human scalps. These strains were identified as 14 Bacillus megaterium, 1 Bacillus subtilis, 3 Staphylococcus aureus and 2 Staphylococcus saprophyticus. Two genuses are Gram-positive. In order to search for antimicrobial substances from natural plants, eighteen plant materials being made of perilla leaf as well as spices including garlic and ginger were used. The effects of these vegetable and fruit juices on the growth of scalp bacterial strains were investigated. Garlic and lemon juices showed antimicrobial activities on the growth of twenty bacterial strains belonging to 4 kinds of species. Onion, spring onion and leek juices inhibited the growth of only one bacterial strains Bacillus megeterium MS13.

• 식물병연구
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• 제19권1호
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• pp.12-20
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• 2013

상추 균핵병균에 대하여 길항력을 나타내는 세균을 분리하기 위해 2007년부터 2008년에 걸쳐 초봄에 경기도 남양주시 관내 상추 시설재배지에서 균핵병이 발병한 포장의 토양을 수집하였다. 수집한 토양을 희석평판법을 이용하여 총 196개의 세균을 분리하였고, 분리한 세균을 실험실에서 균핵병균(Sclerotinia sclerotiorum)과의 대치배양을 통해 균사생장억제율이 80% 내외로 우수한 길항력을 나타내는 26개 균주를 선발하였다. 선발된 26개 균주를 16S rDNA염기서열분석으로 동정한 결과 Bacillus megaterium, B. cereus, B. muralis, B. velezensis, Arthrobacter nicotianae, A. oryzae, Pseudomonas fuscovaginae, P. flavescens, Stenotrophomonas maltophilia, Sphingobacterium faecium으로 동정되었다. 길항세균의 균사생장억제율이 높게 나타낸 10개 균주를 대상으로 여름철 포트실험을 통해 균핵 생존에 미치는 영향을 조사한 결과 B. cereus(C210)와 B. megaterium(DK6)이 각각 20%와 35%의 균핵 생존율을 나타냈다. 겨울철에 온실 내에서 포트실험을 통해 상추 균핵병에 대해 방제효과를 조사한 결과 대조구가 80%의 발병율을 나타냈으나, 26개 균주 중 9개 균주가 20% 정도의 발병율을 나타내 향후 상추 균핵병의 생물적 방제를 위한 길항세균으로서의 활용가치가 기대된다.

• Journal of Microbiology and Biotechnology
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• 제1권2호
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• pp.102-110
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• 1991

Alkalophilic Bacillus sp. YJ-451, which was isolated from soil at several area in Korea, produced a novel type of bacteriolytic enzyme (cell wall peptidoglycan hydrolase) extracellulary. The cell wall hydrolytic activity was identified as a clear zone on sodium dodecyl sulfate polyacrylamide gel electrophoresis containing 0.2% (w/v) cell wall of Bacillus sp. as substrate. This enzyme was successively purified 66 fold with 3.2% yield in culture broth by ammonium sulfate precipitation, CM-cellulose column chromatography, and gel filtration, followed by hydroxylapatite column chromatography. The molecular weight of the purified enzyme was estimated to be 27,000 by sodium dodecyl sulfate polyacrylamide gel electrophoresis and gel filtration column chromatography. The optimum pH and temperature for the activity of the enzyme were pH 10.0 and $50^{\circ}C$, respectively. The enzyme was stable between pH 5.0 and 10.0 and up to $40^{\circ}C$. Among the microorganisms used in this experiment the enzyme was active against most of gram negative strains and the genus Bacillus such as B. megaterium, B. licheniformis, B. circulans, B. pumilus, B. macerans, B. polymyxa. The release of dinitrophenylglutamic acid but not reducing group from cell wall peptidoglycan digested by the enzyme suggested that the enzyme is a kind of peptidase which hydrolyzes the peptide bond at the amino group of D-glutamic acid in the peptidoglycan.

• Journal of Microbiology and Biotechnology
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• 제23권5호
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• pp.614-622
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• 2013

Daqu, a traditional fermentation starter, has been used to produce attractively flavored foods such as vinegar and Chinese liquor for thousands of years. Although Bacillus spp. are one of the dominant microorganisms in Daqu, more precise information is needed to reveal why and how Bacillus became dominant in Daqu, and next, to assess the impact of Bacillus sp. on Daqu and its derived products. We combined culture-dependent and culture-independent methods to study the ecology of Bacillus during Daqu incubation. Throughout the incubation, 67 presumptive Bacillus spp. isolates were obtained, 52 of which were confirmed by 16S rDNA sequencing. The identified organisms belonged to 8 Bacillus species: B. licheniformis, B. subtilis, B. amyloliquefaciens, B. cereus, B. circulans, B. megaterium, B. pumilus, and B. anthracis. A primer set specific for Bacillus and related genera was used in a selective PCR study, followed by a nested DGGE PCR targeting the V9 region of the 16S rDNA. Species identified from the PCR-DGGE fingerprints were related to B. licheniformis, B. subtilis, B. amyloliquefaciens, B. pumilus, B. benzoevorans, and B. foraminis. The predominant species was found to be B. licheniformis. Certain B. licheniformis strains exhibited potent antimicrobial activities. The greatest species diversity occurred at the Liangmei stage of Daqu incubation. To date, we lack sufficient knowledge of Bacillus distribution in Daqu. Elucidating the ecology of Bacillus during Daqu incubation would enable the impact of Bacillus on Daqu to be accessed, and the quality and stabilization of Daqu-derived products to be optimized.

• Journal of Microbiology and Biotechnology
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• 제23권5호
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• pp.707-714
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• 2013

Microbially induced calcium carbonate precipitation (MICCP) is a naturally occurring biological process that has various applications in remediation and restoration of a range of building materials. In the present investigation, five ureolytic bacterial isolates capable of inducing calcium carbonate precipitation were isolated from calcareous soils on the basis of production of urease, carbonic anhydrase, extrapolymeric substances, and biofilm. Bacterial isolates were identified as Bacillus megaterium, B. cereus, B. thuringiensis, B. subtilis, and Lysinibacillus fusiformis based on 16S rRNA analysis. The calcium carbonate polymorphs produced by various bacterial isolates were analyzed by scanning electron microscopy, confocal laser scanning microscopy, X ray diffraction, and Fourier transmission infra red spectroscopy. A strain-specific precipitation of calcium carbonate forms was observed from different bacterial isolates. Based on the type of polymorph precipitated, the technology of MICCP can be applied for remediation of various building materials.

• 한국인쇄학회지
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• 제19권2호
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• pp.30-36
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• 2001

The microbiodeterioration of paper material like books, archival material, manuscripts both illustrated and written, decorative wall papers, etc. is a serious problem throughout the world in museums, libraries, archives, etc. where these materials are placed. The major ecological fungi from record materials of library with ancient achieves were isolated and identified as Aspergillus niger, Aspergillus oryzae, Neurospora sitophile. Mucor mucedo, Mucor rouxii, Penicillium notatum, Rhizopus delema, Rhizopus nigracans, Thanmnidium elegans, Tricodema viridae, Bacillus cereus. Bacillus megaterium, Enterrobacter aerogenes, and Staphylococcus auerus. This study was carried out to investigate the antigungal activities of essential oils. The Essential oils from herb were obtained by vapor distillation method, and the antimicrobial activities were examined with ten fungi & four bacteria. It was found to have activity against microorganisms isolated from a achieves stack rooms.

• 대한환경공학회지
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• 제33권11호
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• pp.812-820
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• 2011

본 연구는 이산화탄소의 생광물화에 관한 연구로 광물별 생광물화 특성을 알아보기 위하여 CaO, MgO, $SiO_2$ 수용액을 이용하여 bottle test를 진행하였으며, 슬러지 고화물을 이용하여 batch test를 진행하였다. 대상 미생물은 생광물화 관련 미생물 중 Bacillus Megaterium과 Bacillus pasteurii 종을 이용하여 실험을 진행하였다. 단일 수용액을 사용한 실험에서는 CaO, MgO, $SiO_2$에 대해 각각 1.1, 4.0, 0.3 mmol의 이산화탄소 가스가 감소되었다. 혼합 수용액을 사용한 실험에서는 Ca + Mg, Ca + Si, Mg + Si에 대해 각각 2.7, 1.8, 2.3 mmol의 이산화탄소가 감소되었다. 슬러지 고화물을 이용한 batch test의 경우, 슬러지 고화물의 양과 주입된 이산화탄소 가스의 농도에 따라 각각 다른 이산화탄소의 소모를 보였다. XRD 분석 결과, Ca 이온이 존재할 때 $CaCO_3$ (Calcite)가 가장 잘 형성되었으며, SEM 분석 결과, 생광물화 반응 후 대상매체에 단립화된 결정이 더 많이 형성된 것을 확인할 수 있었다. 본 연구 결과 생광물화 기술이 이산화탄소를 저감하기 위한 기술로 활용될 수 있음을 알 수 있었다.