• 제어로봇시스템학회논문지
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• 제11권4호
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• pp.353-362
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• 2005

This article describes a state-of-the art review in nano-biomanipulation technologies. Nanomanipulation of biological objects enables an in-depth study of single molecules such as DNA and RNA, and of biophysical events at the molecular level like molecular motors. Controlled nanomanipulation is challenging but essential for precisely engineering biomolecules or cells and for manufacturing functional nano-biosystems. In this paper, we summarize several contact, non-contact and hybrid methods available for nanomanipulation of biological objects. Advantages currently available methods and their limitations are also compared. Finally, we discuss possible applications of nano-biomanipulation technologies to life science and molecular medicine including cell biology, genetic engineering, biophysics, and biochemistry.

• Journal of Microbiology and Biotechnology
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• 제33권8호
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• pp.1013-1022
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• 2023

Arbuscular mycorrhizal fungi (AMF) are widespread soil endophytic fungi, forming mutualistic relationships with the vast majority of land plants. Biochar (BC) has been reported to improve soil fertility and promote plant growth. However, limited studies are available concerning the combined effects of AMF and BC on soil community structure and plant growth. In this work, a pot experiment was designed to investigate the effects of AMF and BC on the rhizosphere microbial community of Allium fistulosum L. Using Illumina high-throughput sequencing, we showed that inoculation of AMF and BC had a significant impact on soil microbial community composition, diversity, and versatility. Increases were observed in both plant growth (the plant height by 8.6%, shoot fresh weight by 12.1%) and root morphological traits (average diameter by 20.5%). The phylogenetic tree also showed differences in the fungal community composition in A. fistulosum. In addition, Linear discriminant analysis (LDA) effect size (LEfSe) analysis revealed that 16 biomarkers were detected in the control (CK) and AMF treatment, while only 3 were detected in the AMF + BC treatment. Molecular ecological network analysis showed that the AMF + BC treatment group had a more complex network of fungal communities, as evidenced by higher average connectivity. The functional composition spectrum showed significant differences in the functional distribution of soil microbial communities among different fungal genera. The structural equation model (SEM) confirmed that AMF could improve the microbial multifunctionality by regulating the rhizosphere fungal diversity and soil properties. Our findings provide new information on the effects of AMF and biochar on plants and soil microbial communities.

• Bulletin of the Korean Chemical Society
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• 제33권2호
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• pp.647-650
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• 2012

Promising anticancer compounds of the type 1,6-disubstituted 4,5,6,7-tetrahydropyrazolo[3,4-c]pyridin-7-ones were identified. The target compounds were readily synthesized in a large scale via a sequence of reactions starting from the commercially available primary amines. Their in vitro anti-proliferative activity has been evaluated on prostate (DU-145), colon (HT-29 and HCT-116) and melanoma (A375P) human cancer cell lines. The relationships between the structure and the anticancer activity, covering all tested cancer cell lines, revealed that the compound 5c with 2,4-dimethylphenyl substituent at $R^2$ was the most potent with the $IC_{50}$ values in the range as low as 0.16 to $0.40{\mu}M$.

• 대한기계학회논문집A
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• 제26권6호
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• pp.1069-1076
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• 2002

The high temperature creep behavior of heat machine systems such as aircraft engines, boilers and turbines in power plants and nuclear reactor components have been considered as an important and needful fact. There are considerable research results available for the design of high temperature tube materials in power plants. However, few studies on the Initial Strain Method (ISM) capable of securing repair, maintenance, cost loss and life loss have been made. In this method, 3 long time prediction Of high temperature creep characteristics can be dramatically induced through a short time experiment. The purpose of present study is to investigate the high temperature creep lift of Udimet 720, SCM 440-STD61 and 1Cr-0.5Mo steel using the ISM. The creep test was performed at 40$0^{\circ}C$ to $700^{\circ}C$ under a pure loading. In the prediction of creep life for each materials, the equation of ISM was superior of Larson-Miller Parameter(LMP). Especially, the long time prediction of creep life was identified to improve the reliability.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2007년도 추계학술대회 논문집
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• pp.1158-1164
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• 2007

Recently, the demand on the practical application of life-cycle cost effectiveness for design and rehabilitation of civil infrastructure is rapidly growing unprecedentedly in civil engineering practice. Accordingly, it is expected that the life-cycle cost in the 21st century will become a new paradigm for all engineering decision problems in practice. However, in spite of impressive progress in the researches on the LCC, so far, most researches in Koreahave only focused on roadway bridges, which are not applicable to railway bridges. Thus, this paper presents the formulation models and methods for uncertainty-based LCCA for railroad bridges consideringboth objective statistical data available in the agency database of railroad bridges management and subjective data obtained form interviews with experts of the railway agency, which are used to anew uncertainty-based expected maintenance/repair costs including lifetime indirect costs. For reliable assessment of the life-cycle maintenance/repair costs, statistical analysis considering maintenance history data and survey data including the subjective judgments of railway experts on maintenance/management of railroad bridges, are performed to categorize critical maintenance items and associated expected costs and uncertainty-based deterioration models are developed. Finally, the formulation for simulation-based LCC analysis of railway bridges with uncertainty-based deterioration models are applied to the design-decision problem, which is to select an optimal bridge type having minimum Life-Cycle cost among various railway bridges types such as steel plate girder bridge, and prestressed concrete girder bridge in the basic design phase.

• 유통과학연구
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• 제15권11호
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• pp.31-39
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• 2017

Purpose - This research aims to examine the influence of social capital formed on SNS on SNS users' life satisfaction. This research divides social capital into bridging and bonding social capital based on Social Capital Theory and suggests that each type of social capital will have a positive impact on SNS users' life satisfaction. In addition, this research suggests the self-construal of SNS users as a moderating variable based on Self-Construal Theory. Research design, data, and methodology - To test the hypotheses presented, an online survey was conducted with adult participants. An online research company was hired to conduct an online survey; survey participants were volunteer adult participants residing in Korea. And data was analyzed using SPSS. Results - Analysis results showed that the impact of bonding social capital on life satisfaction was not statistically different between SNS users of differing self-construals(independent vs. interdependent self-construal). However, this study also determined that the impact of bridging social capital on life satisfaction was greater for SNS users with an interdependent self-construal as opposed to those with an independent self-construal. Conclusions - The results of this study expand the scope of available research on social capital formed on SNS and provide practical implications for SNS providers.

• Mycobiology
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• 제45권3호
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• pp.204-208
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• 2017

Nosema ceranae is an obligate intracellular fungal parasite that causes mortality in honey bees and enhances the susceptibility of honey bees to other pathogens. Efficient purification of Nosema spores from the midgut of infected honey bees is very important because Nosema is non-culturable and only seasonably available. To achieve a higher yield of spores from honey bees, in this study, we considered that the initial release of spores from the midgut tissues was the most critical step. The use of 2 mm beads along with enzymatic treatment with collagenase and trypsin enhanced the homogenization of tissues and the yield of released spores by approximately 2.95 times compared with the use of common 3 mm beads alone. The optimal time for the enzyme treatment was determined to be 1 hr as measured by the yield and viability of the spores. A one-step filtration using a filter paper with an $8-11{\mu}m$ pore size was sufficient for removing cell debris. This method may be useful to purify not only N. ceranae spores but also other Nosema spp. spores.

• Journal of Microbiology and Biotechnology
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• 제22권7호
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• pp.902-906
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• 2012

Expressed sequence tags (ESTs) from the Antarctic green algae Pyramimonas gelidicola were analyzed to obtain molecular information on cold acclimation of psychrophilic microorganisms. A total of 2,112 EST clones were sequenced, generating 222 contigs and 219 singletons, and 200 contigs and 391 singletons from control ($4^{\circ}C$) and cold-shock conditions ($-2^{\circ}C$), respectively. The complete EST sequences were deposited to the DDBJ EST database (http://www.ddbj.nig.ac.jp/index-e.html) and the nucleotide sequences reported in this study are available in the DDBJ/EMBL/GenBank. These EST databases of Antarctic green algae can be used in a wide range of studies on psychrophilic genes expressed by polar microorganisms.

• 한국축산식품학회지
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• 제44권3호
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• pp.515-532
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• 2024

Taste is one of the five senses that detect vital information about what we are eating. Comprehending taste is crucial for enhancing the flavor of foodstuffs and high-protein foods like meat. Umami has global recognition as the fifth elementary taste, alongside sweetness, sourness, saltiness, and bitterness. Umami compounds are known to enhance the sensation of recognized flavors such as salty, sweet, bitter, and others. This could end up in greater food consumption by consumption by consumers. With the rising global population, meat consumption is rising and is projected to double by 2025. It is crucial to comprehend the umami mechanism of meat and meat products, identify novel compounds, and employ laboratory methodologies to gather varied information. This knowledge will aid in the development of new consumer products. Although very limited information is available on umami taste and compounds in meat through research data. This article discusses recent advancements in umami compounds in other foodstuff as well as meat to aid in designing future research and meat product development. Moreover, another objective of this review is to learn present techniques in foodstuffs to enhance umami taste and utilize that knowledge in meat products.

• 한국생활과학회지
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• 제22권1호
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• pp.231-243
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• 2013

This study intends to suggest basic data for the activation of rural activities in dietary life education, through a survey of adolescents on rural activities in dietary life education. It was found that 63.2% of subject students had the experience of rural activities; and that as many as 35.2% among the students who experienced rural activities participated in the activities 5 times or more. And the survey showed that rural activities were led most frequently by a teacher in charge (37.9%), and rather rarely by a nutrition teacher (15.4%) or an expert in dietary life education (7.7%). As for food ingredients that adolescents experienced during rural activities, they were composed of easily available good cookers that are common in the Korean diet, and, among them, vegetables were experienced most, followed by cereals and fruits. On the other hand, as for food ingredients preferred by adolescents, it was found that boy students preferred meat, while girl students preferred fruits. And as for improvements in dietary life, it was found that making efforts to have good dietary habits had the highest score, which shows that rural activities contributed to the improvement in adolescents' dietary life. Therefore, given the fact that rural activities go some way towards improving adolescents' dietary life, it is advisable that more opportunities for experiencing rural activities be provided to adolescents in the future so as to help them have good dietary habits.