Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Combined Application Effects of Arbuscular Mycorrhizal Fungi and Biochar on the Rhizosphere Fungal Community of Allium fistulosum L.

  • Chunxiang Ji (Schools of Life Sciences, Anhui Agricultural University) ;
  • Yingyue Li (Schools of Life Sciences, Anhui Agricultural University) ;
  • Qingchen Xiao (Schools of Life Sciences, Anhui Agricultural University) ;
  • Zishan Li (Schools of Life Sciences, Anhui Agricultural University) ;
  • Boyan Wang (Schools of Life Sciences, Anhui Agricultural University) ;
  • Xiaowan Geng (Schools of Life Sciences, Anhui Agricultural University) ;
  • Keqing Lin (Schools of Life Sciences, Anhui Agricultural University) ;
  • Qing Zhang (Schools of Life Sciences, Anhui Agricultural University) ;
  • Yuan Jin (Schools of Life Sciences, Anhui Agricultural University) ;
  • Yuqian Zhai (Schools of Life Sciences, Anhui Agricultural University) ;
  • Xiaoyu Li (Schools of Life Sciences, Anhui Agricultural University) ;
  • Jin Chen (Schools of Life Sciences, Anhui Agricultural University)
  • Received : 2023.03.20
  • Accepted : 2023.06.07
  • Published : 2023.08.28
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Abstract

Arbuscular mycorrhizal fungi (AMF) are widespread soil endophytic fungi, forming mutualistic relationships with the vast majority of land plants. Biochar (BC) has been reported to improve soil fertility and promote plant growth. However, limited studies are available concerning the combined effects of AMF and BC on soil community structure and plant growth. In this work, a pot experiment was designed to investigate the effects of AMF and BC on the rhizosphere microbial community of Allium fistulosum L. Using Illumina high-throughput sequencing, we showed that inoculation of AMF and BC had a significant impact on soil microbial community composition, diversity, and versatility. Increases were observed in both plant growth (the plant height by 8.6%, shoot fresh weight by 12.1%) and root morphological traits (average diameter by 20.5%). The phylogenetic tree also showed differences in the fungal community composition in A. fistulosum. In addition, Linear discriminant analysis (LDA) effect size (LEfSe) analysis revealed that 16 biomarkers were detected in the control (CK) and AMF treatment, while only 3 were detected in the AMF + BC treatment. Molecular ecological network analysis showed that the AMF + BC treatment group had a more complex network of fungal communities, as evidenced by higher average connectivity. The functional composition spectrum showed significant differences in the functional distribution of soil microbial communities among different fungal genera. The structural equation model (SEM) confirmed that AMF could improve the microbial multifunctionality by regulating the rhizosphere fungal diversity and soil properties. Our findings provide new information on the effects of AMF and biochar on plants and soil microbial communities.

Keywords

Acknowledgement

This research was supported by the Key Research and Development Program of Anhui Province (202204c06020021) and the Natural Science Foundation of China (U21A20235 and 32201308).

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