• Molecules and Cells
• /
• 제19권3호
• /
• pp.398-401
• /
• 2005

The bacterial enzyme UDP-N-acetylglucosamine enolpyruvyl transferase catalyzes the first committed step of peptidoglycan biosynthesis, i.e., transfer of enolpyruvate from phosphoenolpyruvate to UDP-N-acetyl-glucosamine. We have overexpressed the enzyme from Haemophilus influenzae in Escherichia coli and crystallized it in the apo-form, as well as in a complex with UDP-N-acetylglucosamine and fosfomycin using ammonium sulfate as the precipitant. X-ray diffraction data from a crystal of the apo-form were collected to $2.8{\AA}$ resolution at 293 K. The crystal quality was improved by co-crystallization with UDP-N-acetylglucosamine and fosfomycin. X-ray data to $2.2{\AA}$ have been collected at 100 K from a flash-frozen crystal of the complex. The complex crystals belong to the orthorhombic space group I222 (or $I2_12_12_1$) with unit-cell parameters of a = 63.7, b = 124.5, and $c=126.3{\AA}$. Assuming a monomer of the recombinant enzyme in the crystallographic asymmetric unit, the calculated Matthews parameter ($V_M$) is $2.71{\AA}^3Da^{-1}$ and solvent content is 54.6%.

• 한국수산과학회지
• /
• 제31권5호
• /
• pp.609-621
• /
• 1998

1. 연구지역내 sand ridge들은 10$\~$20 m 높이의 비대칭형태이며, 등수심선과 평행하게 발달해 있고, sand ridge의 경사진 부분에서는 지역에 따라 sand wave 형태의 이미지가 나타나는 것으로 보아 현재 다소 활동성이 있는 것으로 보인다. 2. Ridge 주변 위치별 입도, 물리적 성질, 음파전달속도 값은 차이가 크지 않았는데, 이것은 본 연구지역의 주 퇴적상인 사질의 퇴적층이 판의 형태로 넓게 분포하고 있기 때문이다. 3. 코어 하부에는 해수면이 상승하면서 생성된 transgressive sand sheet 퇴적상의 특징을 지시하는 40$\~$200cm 두께의 자갈, 패각편, 조립사가 혼재된 퇴적상이 나타난다. 이들 사질의 분급이 양호하지 않은 이유는 해수면이 상승하면서 조류나 고에너지 환경에서 재동(reworking)되어 퇴적된 palimpsest와 관련된 것으로 사료된다.

• Bulletin of the Korean Chemical Society
• /
• 제27권4호
• /
• pp.568-572
• /
• 2006

To study the effect of sequence on DNA structure, the two decamer crystal structures one alternating,d(GTACGCGTAC), and the other non-alternating, d(GGCCGCGGCC), were solved. Crystals of both decamers belong to the hexagonal space group $P6_122$, with one strand in the asymmetric unit. The unit cell constants of the alternating decamer are a = b = 39.26 $\AA$, c = 77.70 $\AA$. The structure was refined with 1,828 reflections from 8.0 to 2.0 Aresolution to an R value of 21.3% with all DNA atoms and 63 water molecules. The isomorphous non-alternating decamer had unit cell dimensions of a = b = 39.05 $\AA$, c = 82.15 $\AA$. The structure was refined with 2,423 reflections from 8.0 to 2.0 $\AA$ resolution to a final R value of 22.2% for all DNA atoms and 65 water molecules. Although the average helical parameters of the decamers are typical of A-DNAs, there are some minor differences between them. The helical twist, rise, x-displacement, inclination and roll alternate in the alternating decamer, but do not in the non-alternating decamer. The backbone conformations in both structures show some differences; the residue G(7) of the alternating decamer is trans for $\alpha$ and $\gamma$ while the trans conformations are observed at the residue G(8) of the non-alternating decamer.

• Journal of Microbiology and Biotechnology
• /
• 제29권3호
• /
• pp.500-505
• /
• 2019

Staphylococcus aureus is a round-shaped, gram-positive bacterium that can cause numerous infectious diseases ranging from mild infections such as skin infections and food poisoning to life-threatening infections such as sepsis, endocarditis and toxic shock syndrome. Various antibiotic-resistant strains of S. aureus have frequently emerged, threatening human lives significantly. Despite much research on the genetics of S. aureus, many of its genes remain unknown functionally and structurally. To counteract its toxins and to prevent the antibiotic resistance of S. aureus, our understanding of S. aureus should be increased at the proteomic scale. SAV0927 was first sequenced in an antibiotic resistant S. aureus strain. The gene is a conserved hypothetical protein, and its homologues appear to be restricted to Firmicutes. In this study, we determined the crystal structure of SAV0927 at $2.5{\AA}$ resolution. The protein was primarily dimeric both in solution and in the crystals. The asymmetric unit contained five dimers that are stacked linearly with ${\sim}80^{\circ}$ rotation by each dimer, and these interactions further continued in the crystal packing, resulting in a long linear polymer. The crystal structures, together with the network analysis, provide functional implications for the SAV0927-mediated protein network.

• 천문학회보
• /
• 제43권2호
• /
• pp.39.2-39.2
• /
• 2018

RR Tel is an interacting binary system in which a hot white dwarf (WD) accretes matter from a Mira variable via gravitational capture of the stellar wind. We present a high-resolution optical spectrum of RR Tel obtained with MIKE at Magellan-Clay telescope, Chile. We find broad emission features at 6825, 7082, 7023, and $7053{\AA}$, which are formed through Raman scattering of far-UV O VI ${\lambda}{\lambda}$ 1032 and $1038{\AA}$, C II ${\lambda}{\lambda}$ 1036 and $1037{\AA}$ with atomic hydrogen. Raman O VI 6825 and 7082 features are characterized by double-peaked profiles indicative of an accretion flow with a characteristic speed ~ 30km/s, whereas the Raman C II features exhibit a single Gaussian profile with FWHM ${\sim}10{\AA}$. Monte Carlo simulations for Raman O VI and C II are performed by assuming that the emission nebula around the WD consists of the inner O VI disk with a representative scale of 1 AU and the outer part with C II sphere. The best fit for Raman profiles is obtained with an asymmetric matter distribution of the O VI disk, the mass loss rate of the cool companion ${\dot{M}}{\sim}2{\times}10^{-6}M_{{\odot}/yr}$ and the wind terminal velocity v~10 km/s. We also find O VI doublet at 3811 and $3834{\AA}$, which are blended with other emission lines. Our profile decomposition shows that the O VI ${\lambda}{\lambda}$ 3811, 3834 doublet have a single Gaussian profile with a width ~ 25 km/s. A comparison of the restored fluxes of C II ${\lambda}{\lambda}$ 1036 and 1037 from Raman C II features with the observed C II ${\lambda}1335$ leads to an estimate of a lower bound of N(CII) > $9.87{\times}10^{13}cm^{-2}$ toward RR Tel, which appears consistent with the presumed distance D ~ 2.6 kpc.

• Journal of Astronomy and Space Sciences
• /
• 제40권3호
• /
• pp.101-111
• /
• 2023

We conducted a statistical study of polar mesospheric summer echoes (PMSEs) in relation to magnetic local time (MLT), considering the geomagnetic conditions using the K-index (or K). Additionally, we performed a case study to examine the velocity profile, specifically for high velocities (≥ ~100 m/s) varying with high temporal resolution at high K-index values. This study utilized the PMSE data obtained from the mesosphere-stratosphere-troposphere radar located in Esrange, Sweden (63.7°N, 21°E). The change in K-index in terms of MLT was high (K ≥ 4) from 23 to 04 MLT, estimated for the time PMSE was present. During the near-midnight period (0-4 MLT), both PMSE occurrence and signal-to-noise ratio (SNR) displayed an asymmetric structure with upper curves for K ≥ 3 and lower curves for K < 3. Furthermore, the occurrence of high velocities peaked at 3-4 MLT for K ≥ 3. From case studies focusing on the 0-3 MLT period, we observed persistent eastward-biased high velocities (≥ 200 m/s) prevailing for ~18 min. These high velocities were accompanied with the systematic motion of profiles at 85-88 km, including large shear formation. Importantly, the rapid variations observed in velocity could not be attributed to neutral wind effects. The present findings suggest a strong substorm influence on PMSE, especially in the midnight and early dawn sectors. The large zonal drift observed in PMSE were potentially energized by local electromagnetic fields or the global convection field induced by the electron precipitation during substorms.

• 천문학회지
• /
• 제56권1호
• /
• pp.11-22
• /
• 2023

We report the result of a high-resolution spectroscopic study on seven magnesium (Mg) enhanced stars. The high Mg abundances in these stars imply that they were born in an environment heavily affected by the nucleosynthesis products of massive stars. We measure abundances of 16 elements including Mg and they show various abundance patterns implying their diverse origin. Three of our program stars show a very high Mg to Si ratio ([Mg/Si] ≈ 0.18-0.25), which might be well explained by fall-back supernovae or by supernovae with rapid rotating progenitors having an initial mass higher than about 20 M_⊙. Another three of our program stars have high light to heavy s-process element ratios ([Y/Ba] ≈ 0.30-0.44), which are consistent with the theoretical prediction of the nucleosynthesis in rapidly rotating massive stars with an initial mass of about M = 40 M_⊙. We also report a star having both high Y ([Y/Fe] = 0.2) and Ba ([Ba/Fe] = 0.28) abundance ratios, and it also shows the highest Zn abundance ratio ([Zn/Fe] = 0.27) among our sample, implying the nucleosynthesis by asymmetric supernova explosion induced by very rapid rotation of a massive progenitor having an initial mass between 20 M_⊙ ≲ M ≲ 40 M_⊙. A relative deficiency of odd-number elements, which would be a signature of the pair-instability nucleosynthesis, is not found in our sample.

• 대한자기공명의과학회:학술대회논문집
• /
• 대한자기공명의과학회 2003년도 제8차 학술대회 초록집
• /
• pp.103-105
• /
• 2003

After fifteen years of development, Magnetic Resonance (MR) technology for human imaging and spectroscopy is reaching a refined state with FDA approved 3T clinical products from Siemens, GE, and Philips. Broker has cleared CE approval with a 4T system. Varian supports a 4T system platform as well. Shielded magnets are standard at 3T from GE, Oxford, Magnex, and IGC. A shielded 4T whole body magnet is available from Oxford. Stronger switched gradients and dynamic shim coils, desired at any field, areespecially useful at higher static magnetic fields B0. In addition to the higher currents required for higher resolution slice or volume selection afforded by higher SNR, whole body gradient coils will be driven at increasing slew rates to meet the needs of new cardiac applications and other requirements. For example 3T and 4T systems are now being equipped with 2kV, 500A gradient coils and amplifiers capable of generating 4G/cm in 200msec, over a 67+/-cm bore diameter. High field EPI applications require oscillation rates at 1 kHz and higher. To achieve a benchmark 0.2 ppm shim over a 30cm sphere in a high field magnet, at least four stages of shimming need to be considered. 1) A good high field magnet will be built to a homogeneity spec. falling in the range of 100 to 150 ppm over this 30cm spherical "sweet spot" 2) Most modern high field magnets will also have superconducting shim coils capable of finding 1.5 ppm by their adjustment during system installation. 3) Passive ferro-magnetic shimming combined with 4) active, high order room temperature shim coils (as many as five orders are now being recommended) will accomplish 0.2 ppm over the 30cm sphere, and 0.1 ppm over a human brain in even the highest field magnets for human studies. Safety concerns for strong, fast gradients at any B0 field include acoustic noise and peripheral nerve stimulation. One or more of the mechanical decoupling methods may lead to quieter gradients. Patient positioning relative to asymmetric or short gradient coils may limit peripheral nerve stimulation at higher slew rates. Gradient designs combining a short coil for local speed and strength with a longer coil for coverage are being developed for 3T systems. Local gradients give another approach to maximizing performance over a limited region while keeping within the physiologically imposed dB0/dt performance limits.

• 대한치과교정학회지
• /
• 제35권5호
• /
• pp.398-407
• /
• 2005

본 연구는 안면비대칭이 있는 환자의 측두하악관절원반 형태와 위치를 평가하고 악관절내장증과 안면비대칭 간의 관계를 규명하기 위하여 시행되었다. 남자 10명 및 여자 27명으로 구성된 안면비대칭 환자 37명의 74개의 TMJ MRI를 사용하였으며, 대상 집단의 연령은 12세에서 44세까지의 범위로 평균 21.4세였다. 시상 및 두정 TMJ MRI를 최대감합위 및 최대개구위에서 채득한 후 부분전방변위, 전방변위, 회전 또는 측방원반변위 등 원반변위의 종류를 기록하였으며, 원반의 모양위치, 그리고 원반의 변위 및 회전을 MRI tracing 상에서 판독하였다. 악관절내장증의 증상은 정상, 정복성 전방원반변위(ADDR), 그리고 비정복성 전방원반변위 (ADDNR) 집단으로 분류하였으며, 환자는 양측성 정상. 편측 혹은 양측 내장증 집단으로 분류하였다. 약$70\%$의 환자에서 편측성 또는 양측성 내장증을 보였으며. 통계분석 결과 ADD, 특히 회전성 ADD가 변위측에서 더 높은 빈도로 나타났으나 반대측에서는 원반의 위치가 정상인 경우가 많았다. (p<0.01). 변위측의 원반은 모양의 변형 및 하전방 변위를 유의하게 더 나타냈다. 그러나 반대쪽은 개구시 원반이 수직방향으로 과운동성이 관찰되었다. 이 연구는 안면비대칭 환자에서 ADD의 종류가 원반의 모양, 경사 변위 각도, 변위의 수직거리 및 회전각도와 연관이 있다는 것을 제시한다

• 한국진공학회지
• /
• 제16권4호
• /
• pp.279-285
• /
• 2007

나노크기의 Au-Si을 촉매로 급속화학기상증착법을 이용하여 Si(111) 기판에 성장한 Si 나노선의 구조적인 형태 변화과정과 광학적 특성을 연구하였다. 액상 입자인 Au 나노 점은 기상-액상-고상(vapor-liquid-solid mechanism) 성장법에 의한 Si 나노선 형성 과정에서 촉매로 사용되었다 이 액체 상태인 나노점에 1.0Torr 압력과 $500-600^{\circ}C$ 온도 하에서 $SiH_4$와 $H_2$의 혼합가스를 공급하여 Si 나노선을 형성하였다. <111> 방향으로 형성한 Si 나노선의 형태를 전계방출 주사전자현미경(Field Emission Scanning Electron Microscope)으로 관찰하였다. 특히, 대부분의 나노선이 균일한 크기를 가지고 있으며, Si(111) 기판 표면에서 수직하게 정렬된 것을 확인하였다. 형성된 나노선의 크기를 분석한 결과, 직경과 길이가 각각 60nm와 5um의 분포를 가지는 것을 확인 하였다. 고 분해능 투과전자현미경(High Resolution-Transmission Electron Microscope)을 통해 약 3nm의 다결정 산화층으로 둘러 싸여 있는 Si 나노선이 단결정으로 형성된 것을 관찰하였다. 그리고 마이크로 라만 분광(Micro-Raman Scattering) 실험으로 Si 나노선의 광학적 특성을 분석하였다. 라만 측정결과 Si의 광학 포논(Optical Phonon) 신호가 Si 나노선의 영향으로 에너지가 작은 쪽으로 이동하며, Si 포논 신호의 폭이 비대칭적으로 증가하는 것을 확인 하였다.