• 제목/요약/키워드: astaxanthin accumulation

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Systemic Statistical Optimization of Astaxanthin Inducing Methods in Haematococcus pluvialis cells -Statistical Optimization of Astaxanthin Production in Haematococcus

  • Kim, Sun-Hyoung;Jeong, Sung Eun;Hong, Seong-Joo;Lee, Choul-Gyun
    • 한국해양바이오학회지
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    • 제6권1호
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    • pp.31-40
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    • 2014
  • The production of astaxanthin in the microalga Haematococcus pluvialis has been investigated using a sequential methodology based on the application of two types of statistical designs. The employed preliminary experiment was a fractional factorial design $2^6$ in which the factors studied were: excessive irradiance and nitrate starvation, phosphate deficiency, acetate supplementation, salt stress, and elevated temperature. The experimental results indicate that the amount of astaxanthin accumulation in the cells can be enhanced by excessive irradiance and nitrate starvation whereas the other factors tested did not yield any enhancement. In the subsequent experiment, a central composite design was applied with four variables, light intensity, nitrate, phosphate, and acetate, at five levels each. The optimal conditions for the highest astaxanthin production were found to be $1040{\mu}E/(m^2{\cdot}s)$ light intensity, 0.04 g/L nitrate, 0.31 g/L phosphate, 0.05 g/L acetate concentration.

Ultrastructural changes of Haematococcus pluvialis (Chlorophyta) in process of astaxanthin accumulation and cell damage under condition of high light with acetate

  • He, Bangxiang;Hou, Lulu;Zhang, Feng;Cong, Xiaomei;Wang, Zhendong;Guo, Yalin;Shi, Jiawei;Jiang, Ming;Zhang, Xuecheng;Zang, Xiaonan
    • ALGAE
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    • 제35권3호
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    • pp.253-262
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    • 2020
  • Haematococcus pluvialis is a commercial microalga that can produce high quantities of astaxanthin. Under induced conditions, some important changes in the subcellular structures related to astaxanthin accumulation were observable. For example, a large number of astaxanthin granules, oil structures and starch granules appeared in the thick-walled cells; Astaxanthin granules gradually dissolved into the oil structures and spread throughout the entire cell with the fusion and diffusion process of oil structures during the middle and late stages of induction; The plastoglobules were closed to the newly formed structures, and some plastoglobules would abnormally increase in size under stress. Based on observations of cell damage, the degradation of membrane structures, such as chloroplasts, was found to be the primary form of damage during the early stage of induction. During the middle stage of induction, some transparent holes were exposed in the dissolving astaxanthin granules in the cytoplasm. In thick-walled cells, these transparent holes were covered by oil substances dissolving astaxanthin, thereby avoiding further damage to cells. Given the relatively few oil structures, in non-thick-walled cells, the transparent holes expanded to form multiple transparent areas, eventually resulting in the rupture and death of cells. These results suggested that the high level of synthesis and the wide range diffusion of oil explained the expansion of astaxanthin in H. pluvialis.

배지 조성이 Haematococcus pluvialis의 생장과 Astaxanthin 생산에 미치는 영향 (Effects of Medium Copositions for the Growth and the Astaxanthin Production of Haematococcus pluvialis)

  • 박은경;서문원;이철균
    • 한국미생물·생명공학회지
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    • 제29권4호
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    • pp.227-233
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    • 2001
  • 고부가가치의 천연 astaxanthin의 생산을 위한 Haematococcus pluvialis의 고농도 배양을 수립하기 위하여 세포 농도를 증가시키는 방법과 세포 내 색소를 증가시키는 방법 두 가지에 초점을 맞춰 본 연구를 수행하였다 Hongkong Medium (HKM) Modifed Bolds Basal Medium (MBBM) Modified Bristols Medium(MBM) Proteose-petone Medium (PPM) BG-11 Medium의 여러 배지를 사용하여 비교함으로서 배지 내 탄소원 질소원과 미량원소 등이 세포의 생장과 astaxan-tin 생산에 미치는 영향을 보면 HKM 은 $2.0$\times$10^{6}$ cells /mL의 세포 농도와 최고 6.14 mg/g cell의 astaxanthin content per cell 로 다른 배지 중 가장 높게 나타나므로 세포 농도를 높이기위해서 질소우너이 220 mg/L 인 HKM이 적당하고 색소 형성을 촉진시키기 위해서는 aggregation이 일어나 단위 세포당 astaxanthin 함량이 9.7 mg/g cell 로 높게 나타나는 MBBM이 월등함을 알수 있었다. H. pluvialis의 최적 배양 온도와 pH를 선정하깅 nl하여 $20^{\circ}C$ $25^{\circ}C$, 3$0^{\circ}C$ 온도와 pH 4.5 6.0 7.5 의 배양조건을 수립한 결가 pH $7.5 20^{\circ}C$$25^{\circ}C$에서는 세포생장과 색소 형성이 우수함을 관찰 할 수 있었고 배양에 적당한 20 $^{\circ}C$ 에서 배양하다가 $30^{\circ}C$로 24시간 배양항 heating 효과를 주면 astaxanthin 축적을 촉진시키는데 효과적임을 알수있었다. PPMso의 질소원과 proteose-peptone의 고갈시세포의 생장의특성과 astaxanthin 생산에 미치는 영향은 control 배지에서 세포 농도와 세포의 건조질량이 각각 $0.98$\times$10^{6}$ / cell /mL 와 0.2 g/L astaxanthin의 농도는 1.92 mg/L 단위 세포당 astaxanthin 농도는 9.6 mg/g cell 로 관찰되었다결론적으로 질소원과 peptone이 고갈되면 세포의 생장은 억제되나 astaxanthin의 생산은 촉진됨을 알수 있었으며 세포 생장을 촉진하는 광도 60$\mu$E/($\m^2$s)와 HKM 배지 이용의 1단계와 높은 광도와 MBBM배지를 이용한 색소 생산의 2단계 배양을 최적조건으로 수립하였다.

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Nephroprotective effect of astaxanthin against trivalent inorganic arsenic-induced renal injury in wistar rats

  • Wang, Xiaona;Zhao, Haiyuan;Shao, Yilan;Wang, Pei;Wei, Yanru;Zhang, Weiqian;Jiang, Jing;Chen, Yan;Zhang, Zhigang
    • Nutrition Research and Practice
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    • 제8권1호
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    • pp.46-53
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    • 2014
  • Inorganic arsenic (iAs) is a toxic metalloid found ubiquitously in the environment. In humans, exposure to iAs can result in toxicity and cause toxicological manifestations. Arsenic trioxide ($As_2O_3$) has been used in the treatment for acute promyelocytic leukemia. The kidney is the critical target organ of trivalent inorganic As ($iAs^{III}$) toxicity. We examine if oral administration of astaxanthin (AST) has protective effects on nephrotoxicity and oxidative stress induced by $As_2O_3$ exposure (via intraperitoneal injection) in rats. Markers of renal function, histopathological changes, $Na^+-K^+$ ATPase, sulfydryl, oxidative stress, and As accumulation in kidneys were evaluated as indicators of $As_2O_3$ exposure. AST showed a significant protective effect against $As_2O_3$-induced nephrotoxicity. These results suggest that the mechanisms of action, by which AST reduces nephrotoxicity, may include antioxidant protection against oxidative injury and reduction of As accumulation. These findings might be of therapeutic benefit in humans or animals suffering from exposure to $iAs^{III}$ from natural sources or cancer therapy.

우렁쉥이 껍질 추출물이 보리새우 Cholesterol 축척 및 착색에 미치는 영향 (Effect of Ascidian Tunic Extracts on Cholesterol Accumulation and Pigmentation of Kuruma Prawn, Penaeus japonicus)

  • 최병대;강석중;이강호
    • 한국수산과학회지
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    • 제29권3호
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    • pp.393-408
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    • 1996
  • 우렁쉥이 껍질 색소추출물을 astaxanthin 함량으로 환산하여 100ppm, 200ppm 및 400ppm 첨가 한 후 보리새우를 8주간 사육하여 성장도, 착색정도, 지질 및 총 콜레스테롤 함량 그리고 육과 머리의 지방산조성을 조사하였다. 성장율은 대조구(Diet 1)가 가장 낮아 0.610이었고, carophyll pink 첨가구(Diet 2)가 1.065, 껍질 색소추출물 첨가구인 Diet 3(100ppm), Diet 4(200ppm), Diets (400ppm)는 각각 1.227, 1.292, 1.264로 Diet 4 첨가구가 가장 양호하였다. 총카로테노이드 함량은 대조구가 8주 후 육 및 껍질 중 0.176mg, 1.429mg으로 감소하였으나, 색소추출물구인 Diet 4와 5는 각각 2.337mg, 6.331mg으로 가장 우수하였다. Astaxanthin monoester가 free형보다 착색에 더 영향을 미치는 것으로 나타났다. 육 및 머리 중 지질함량도 Diet 4, 5가 8주 후 각각 1632.7mg, 1691.4mg, 1973.5mg, 2071.1mg으로 가장좋았고, 육 및 머리의 콜레스테롤 축적량은 Diet 2가 8주 후 각각 481.5mg, 199.5mg이었으나, Diet 4는 각각 978.2mg, 563.8mg으로 성장에 직접 영향을 미치는 것으로 나타났다. 머리 지질 중 총지질(TL) 및 중성지질(NL)의 지방산조성은 모노엔산의 함량이 높고 고도불포화지방산(PUFA) 함량은 낮았으나, 이와는 반대로 국성지질(PL)은 모노엔산 함량은 낮고 PUFA 함량은 높아 인지질 중 n-3계 고도불포화지방산의 비율이 높게 나타나 색소추출물이 필수지방산 공급원의 역활을 하는 것으로 나타났다.

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양식 담수어 및 해산어의 사료 Carotenoids 대사의 비교와 체색개선에 미치는 영향 (Comparison of Dietary Carotenoids Metabolism and Effects to Improve the Body Color of Cultured Fresh-water Fishes and Marine Fishes)

  • 하봉석;권문정;박미연;백승한;김수영;백인옥;강석중
    • 한국식품영양과학회지
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    • 제26권2호
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    • pp.270-284
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    • 1997
  • 무지개 송어, 산천어, 뱀장어, 볼락 및 우럭에 대한 사료 carotenoids의 체내대사와 체색 개선효과를 검토하기 위하여, 사료에 ${\beta}-carotene$, lutein, canthaxanthin, astaxanthin 및 ${\beta}-apo-8’-carotenal$을 각각 첨가하여 4 내지 5주간 사육하여 표피의 carotenoids 성분의 변화를 분석, 비교한 결과는 다음과 같다. 무지개 송어 표피의 carotenoids 조성은, zeaxanthin, ${\beta}-carotene$ 및 canthaxanthin이 주성분이였으며, 그 외 lutein, isocryptoxanthin 및 salmoxanthin을 소량 성분으로 함유하며, 표피의 carotenoids 축적율은 canthaxanthin 첨가구에서 높게 나타나 체색 선명화 효과가 가장 컸으며, astaxanthin, ${\beta}-carotene$ 첨가구의 순으로 나타났다. 무지개 송어 표피에서의 carotenoids 대사경로는, ${\beta}-carotene$이 isocryptoxanthin, echinenone 및 canthaxanthin을 경유하여 astaxanthin으로 lutein은 canthaxanthin으로 산화되고, canthaxanthin은 isozeaxanthin을 경유하여 ${\beta}-carotene$으로 환원되며 astaxanthin은 triol을 경유하여 zeaxanthin으로 환원되는 대사경로를 추정할 수 있었다. 산천어 표피의 carotenoids 조성은, zeaxanthin이 주성분이며, 그 외 triol, lutein, tunaxanthin, ${\beta}-carotene$, ${\beta}-cryptoxanthin$ 및 canthaxanthin을 소량 성분으로 함유하며, 표피의 carotenoids 축적율은 canthaxanthin 첨가구에서 높게 나타나 체색 선명화 효과가 가장 컸으며, lutein, ${\beta}-carotene$ 첨가구의 순으로 나타났다. 산천어 표피에서의 carotenoids 대사경로는, ${\beta}-carotene$이 zeaxanthin으로 산화되고, lutein은 tunaxanthin을 경유하여 zeaxanthin으로 환원되고, canthaxanthin은 ${\beta}-carotene$을 경유하여 zeaxanthin으로 산화되며 astaxanthin은 triol을 경유하여 zeaxanthin으로 환원되는 대사경로를 추정할 수 있었다. 뱀장어 표피의 carotenoids 조성은, ${\beta}-carotene$이 주성분이였으며, 그 외 lutein, zeaxanthin 및${\beta}-cryptoxanthin$등이 소량 성분으로 함유하며, 표피의 carotenoids 축적율은 lutein 첨가구에서 높게 나타나 체색 선명화 효과가 가장 컸었고, canthaxanthin astaxanthin 첨가구의 순으로 나타났다. 뱀장어 표피에서의 carotenoids 대사경로는, ${\beta}-carotene$과 lutein은 그대로 축적되며, canthaxanthin은 ${\beta}-carotene$으로 그리고 astaxanthin은 zeaxanthin으로 환원되는 대사경로를 추정 할 수 있었다. 볼락 표피의 carotenoids 조성은, zeaxanthin, ${\beta}-carotene$, tunaxanthin A, tunaxanthin B, tunaxanthin C 및 lutein이 주성분이였으며, 그 외 ${\beta}-cryptoxanthin$, ${\alpha}-cryptoxanthin$, astaxanthin을 소량 성분으로 함유하며, 표피의 carotenoids 축적율은 lutein 첨가구에서 높게 나타나 체색 선명화 효과가 가장 컸으며, ${\beta}-carotene$, canthaxanthin 첨가구의 순으로 나타났다. 볼락 표피에서의 carotenoids의 대사경로는, ${\beta}-carotene$은 lutein으로 산화되며, lutein, canthaxanthin astaxanthin 및 ${\beta}-apo-8'-carotenal$은 zeaxanthin을 경유하여 tunaxanthin으로 각각 산화 및 환원되는 대사경로를 추정할 수 있었다. 우럭 표피의 carotenoids 조성은, ${\beta}-carotene$, astaxanthin 및 zeaxanthin이 주성분이였으며, 그 외 ${\alpha}-cryptoxanthin$, ${\beta}-cryptoxanthin$, lutein 및 canthaxanthin을 소량 성분으로 함유하며, 표피의 carotenoids 축적율은 lutein 첨가구에서 높게 나타나 체색 선명화 효과가 가장 컸으며, canthaxanthin, ${\beta}-carotene$ 첨가구의 순으로 나타났다. 우럭 표피에서의 carotenoids 대사경로는 ${\beta}-carotene$${\beta}-cryptoxanthin$으로 산화되고, lutein은 ${\alpha}-cryptoxanthin$을 경유하여 ${\beta}-carotene$으로 환원되고, canthaxanthin은 ${\beta}-cryptoxanthin$, zeaxanthin을 경유하여 ${\alpha}-cryptoxanthin$으로 환원되며, astaxanthin은 isocryptoxanthin, zeaxanthin을 경유하여 tunaxanthin으로 대사되며, ${\beta}-apo-8’-carotenal$${\beta}-cryptoxanthin$, zeaxanthin을 경유하여 ${\alpha}-cryptoxanthin$으로 환원되는 대사경로를 추정할 수 있었다.

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대사공학기술을 이용한 기능성 carotenoids 고 생산 감자의 개발 현황 (Development of transgenic potato with high content of functional carotenoids by using metabolic engineering)

  • 안미정;배정명;이신우
    • Journal of Plant Biotechnology
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    • 제37권4호
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    • pp.388-393
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    • 2010
  • 감자의 괴경에 비타민 A의 전구물질인 베타 카로텐 또는 항산화활성이 높으나 일반식물에서는 합성되지 않는 astaxanthin과 같은 케토형 카로티노이드의 집적을 위한 연구가 최근 활발하게 진행되고 있다. 현재까지의 연구 결과를 요약하여 보면 $\beta$-carotene의 함량이 $47\;{\mu}g/g$ dry weight으로 대조구에 비하여 3,600배 까지 증가한 결과가 보고되었다. 또한, 해양 미생물로부터 분리한 $\beta$-carotene ketolase 유전자를 도입한 감자의 괴경에서 astaxanthin 등의 케토형 카로티노이드의 함량이 최고 $14\;{\mu}g/g$ dry weight 까지 증가하여 감자의 색깔이 붉은 색으로 변하였다고 보고하였다. 따라서 본 논문에서는 이들 형질전환 감자의 생산을 위하여 도입한 유전자의 종류, 대사공학적 전략, 문제점 및 향후 연구 방향 등에 관하여 논하고자 하였다.

칼납자루이 사료 Carotenoids 대사와 체색개선에 미치는 영향 (Metabolism of Dietary Carotenoids and Effects to Improve the Body Color of Oily Bittering, Acheilognathus koreensis)

  • 백승한;김현영;김수영;정계임;권문정;김종현;하봉석
    • 한국식품영양과학회지
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    • 제28권5호
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    • pp.1099-1106
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    • 1999
  • Effects of dietary carotenoids were investigated on metabolism of the carotenoids, and body pigmen tation in oily bittering, Acheilognathus koreensis. Two weeks later after depletion,oily bitterings were fed the diets supplemented with either lutein, cynthiaxanthin and astaxathin for 4 weeks. Carotenoids distributed to and metabolized in integument were analyed. The carotenoid isolated from the integument of wild oily bittering, composed of 47.2% zeaxanthin, 11.4% lutein epoxide, 11.0% diatoxanthin, 9.7% lutein and 8.3% zeaxanthin epoxide. Meanwhile, two weeks later after depletion, the carotenoid composed of 29.9% crytoxanthin, 19.3% zeaxanthin, 13.2% lutein epoxide, 12.0% diatoxanthin and 8.8% zeaxanthin epoxide. These indicated that zeaxanthin, diatoxanthin, lutein epoxide and zeaxanthin epoxide were actively metabolized in oily bittering, compared to that of other fresh water fish. Total carotenoid content in the integument of wild oily bittering and oily bittering depleted for two weeks was found to be 1.72mg% and 2.08mg%, respectively. Two weeks later after treatment of experimental diet, total carotenoids content was increased to 2.23mg% in lutein, 2.36mg% in cynthiaxanthin and 2.49mg% in astaxanthin supplemented group, which were relatively higher than 2.10mg% in control group. Meanwhile, 4 weeks later, total ca rotenoids content was decreased to 1.76mg% in control, 1.95mg% in lutein, 1.74mg% in cynthiaxanthin and 1.72mg% in astaxanthin supplemented groups. These result indicate that dietary carotenoids were rapidly accumulated and then metabolized to certain metabolites shortly after feeding. Body pigmentation effects of the carotenoids due to accumulation of carotenoids in the integument of oily bittering was the most effectively shown in the astaxanthin supplemented group, followed by cynthiaxanthin and lutein supplemented groups. In the integument of oily bittering, dietary carotenoids were presumably biotrans formed via either oxidative or reductive pathways as presumed the variation of total carotenoid content and carotenoid composition in all experimental groups. The lutein was oxidized either to astaxanthin via doradexanthin and doradexanthin, or to zeaxanthin epoxide via zeaxanthin by oxidative pathway. Cynthiaxanthin was converted either to diatoxanthin and zeaxanthin by reductive pathway and then to zeaxanthin epoxide by oxidative pathway, or it was converted to astaxanthin via diatoxanthin, zeaxan thin and doradexanthin by oxidative pathway. Astaxanthin was converted to doradexanthin and zeaxanthin by reductive pathway and then to zeaxanthin epoxide by oxidative pathway. These results suggest that, oxidative pathway of carotenoids was major metabolic pathway along with reductive path way in fresh water fish.

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Effects of Nutrient Levels on Cell Growth and Secondary Carotenoids Formation in the Freshwater Green Alga, Chlorococcum sp.

  • Liu, Bei-Hui;Haizhang, Dao;Lee, Yuan-Kun
    • Journal of Microbiology and Biotechnology
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    • 제10권2호
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    • pp.201-207
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    • 2000
  • The freshwater green alga Chlorococcum sp. grew on NH_4^{+},{\;}NO_3^{-}$, urea, yeast extract, and peptone as the nitrogen source showing similar pattens of growth and secondary carotenoid (SC) production. However, the most suitable nitrogen source for the induction fo SC was urea. The dffects of nutrient levels (urea, phosphate, sulfate, ferrous iron, and salt) on growth and SC production were stydied by varying the concentration of each nutrient in batch cultures. High biomass production was achieved in cultures containing 20-28 mM urea, 4.8-10 mM phosphate, 1.6 mM sulfate, 70 mM phosphate, 1.6 mM sulfate, 170 mM NACl, and $50{\;}\mu\textrm{M}$ iron. The optimum concentrations of nutrients for biomass and for the SC accumulation in biomass were evaluated and the two media for achieving high biomass production and SC production were thus developed. The extent to which each parameter to stimulate the formation of SC in the alga were varied and the potentially improned SC prodution by manipulating the nutrient levels in the modified media were descussed.

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Stress-induced secondary carotenogenesis in Coelastrella rubescens (Scenedesmaceae, Chlorophyta), a producer of value-added keto-carotenoids

  • Minyuk, Galina;Chelebieva, Elina;Chubchikova, Irina;Dantsyuk, Natalia;Drobetskaya, Irina;Sakhon, Evgenii;Chekanov, Konstantin;Solovchenko, Alexei
    • ALGAE
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    • 제32권3호
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    • pp.245-259
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    • 2017
  • We report on the culture growth and stress-induced secondary carotenogenesis in a biotechnologically promising but largely unexplored chlorophyte Coelastrella rubescens strain Vinatzer/Innsbruck V 195. Changes in the cell morphometry, biomass accumulation, its carotenoid and fatty acid profiles were followed in the cultures supplemented with either inorganic ($CO_2$) or organic (sodium acetate) carbon on the background of low-pH stress. Collectively, the results of the study characterize C. rubescens as a biotechnologically promising, potentially double-purpose organism. It produces several secondary keto-carotenoids with a considerable proportion of astaxanthin and canthaxanthin. At the same time, the cell lipid fatty acid profile of this microalga is suitable for obtaining a high-quality biodiesel complying with the strictest EN14214 European standard.