Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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Systemic Statistical Optimization of Astaxanthin Inducing Methods in Haematococcus pluvialis cells -Statistical Optimization of Astaxanthin Production in Haematococcus

  • Kim, Sun-Hyoung (National Marine Bioenergy Research Center & Department of Biological Engineering, Inha University) ;
  • Jeong, Sung Eun (National Marine Bioenergy Research Center & Department of Biological Engineering, Inha University) ;
  • Hong, Seong-Joo (National Marine Bioenergy Research Center & Department of Biological Engineering, Inha University) ;
  • Lee, Choul-Gyun (National Marine Bioenergy Research Center & Department of Biological Engineering, Inha University)
  • Received : 2014.06.30
  • Accepted : 2014.07.31
  • Published : 2014.06.30
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Abstract

The production of astaxanthin in the microalga Haematococcus pluvialis has been investigated using a sequential methodology based on the application of two types of statistical designs. The employed preliminary experiment was a fractional factorial design $2^6$ in which the factors studied were: excessive irradiance and nitrate starvation, phosphate deficiency, acetate supplementation, salt stress, and elevated temperature. The experimental results indicate that the amount of astaxanthin accumulation in the cells can be enhanced by excessive irradiance and nitrate starvation whereas the other factors tested did not yield any enhancement. In the subsequent experiment, a central composite design was applied with four variables, light intensity, nitrate, phosphate, and acetate, at five levels each. The optimal conditions for the highest astaxanthin production were found to be $1040{\mu}E/(m^2{\cdot}s)$ light intensity, 0.04 g/L nitrate, 0.31 g/L phosphate, 0.05 g/L acetate concentration.

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References

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