• Title/Summary/Keyword: artificial culture

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The Biodegradation of Mixtures of Benzene,Phenol,and Toluene by Mixed and Monoculture of Bacteria (단일배양 및 혼합배양에 의한 Benzene, Phenol 및 Toluene 혼합물의 생분해)

  • Lee, Chang-Ho;Oh, Hee-Mock;Kwon, Tae-Jong;Kwon, Gi-Seok;Kim, Seong-Bin;Kho, Yung-Hee;Yoon, Byung-Dae
    • Microbiology and Biotechnology Letters
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    • v.22 no.6
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    • pp.685-691
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    • 1994
  • The biodegradation of aromatic compounds by mixed and monoculture was investigated in an artificial wastewater containing 500 mg/l of benzene(B), phenol(P), and toluene(T) in various combinations. None of three strains utilized P-xylene(X) as a carbon source, but they grew well on p-xylene in mixtures with benzene and toluene. In the mixed culture on mixed substrate, the length of lag phase was different depending on the nature of mixture. Cell growths of Flavobac- terium sp. BEN2 and Acinetobacter sp. GEM63 were inhibited in the presence of a 500 mg/l of phenol. When the mixed culture of three strains was cultured in a bench-scale reactor containing artificial wastewater, each of benzene, phenol, and toluene was not detected at 30 hrs, 50 hrs, and 12 hrs after incubation in the treatment. The removal rates of COD$_{t}$(total COD) and COD$_{s}$,(soluble COD) of upper phase after centrifugation during early 50 hrs were ca. 80% and ca. 93.8%, respectively.

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Performance Comparison Analysis of Artificial Intelligence Models for Estimating Remaining Capacity of Lithium-Ion Batteries

  • Kyu-Ha Kim;Byeong-Soo Jung;Sang-Hyun Lee
    • International Journal of Advanced Culture Technology
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    • v.11 no.3
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    • pp.310-314
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    • 2023
  • The purpose of this study is to predict the remaining capacity of lithium-ion batteries and evaluate their performance using five artificial intelligence models, including linear regression analysis, decision tree, random forest, neural network, and ensemble model. We is in the study, measured Excel data from the CS2 lithium-ion battery was used, and the prediction accuracy of the model was measured using evaluation indicators such as mean square error, mean absolute error, coefficient of determination, and root mean square error. As a result of this study, the Root Mean Square Error(RMSE) of the linear regression model was 0.045, the decision tree model was 0.038, the random forest model was 0.034, the neural network model was 0.032, and the ensemble model was 0.030. The ensemble model had the best prediction performance, with the neural network model taking second place. The decision tree model and random forest model also performed quite well, and the linear regression model showed poor prediction performance compared to other models. Therefore, through this study, ensemble models and neural network models are most suitable for predicting the remaining capacity of lithium-ion batteries, and decision tree and random forest models also showed good performance. Linear regression models showed relatively poor predictive performance. Therefore, it was concluded that it is appropriate to prioritize ensemble models and neural network models in order to improve the efficiency of battery management and energy systems.

Development of YOLOv5s and DeepSORT Mixed Neural Network to Improve Fire Detection Performance

  • Jong-Hyun Lee;Sang-Hyun Lee
    • International Journal of Advanced Culture Technology
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    • v.11 no.1
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    • pp.320-324
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    • 2023
  • As urbanization accelerates and facilities that use energy increase, human life and property damage due to fire is increasing. Therefore, a fire monitoring system capable of quickly detecting a fire is required to reduce economic loss and human damage caused by a fire. In this study, we aim to develop an improved artificial intelligence model that can increase the accuracy of low fire alarms by mixing DeepSORT, which has strengths in object tracking, with the YOLOv5s model. In order to develop a fire detection model that is faster and more accurate than the existing artificial intelligence model, DeepSORT, a technology that complements and extends SORT as one of the most widely used frameworks for object tracking and YOLOv5s model, was selected and a mixed model was used and compared with the YOLOv5s model. As the final research result of this paper, the accuracy of YOLOv5s model was 96.3% and the number of frames per second was 30, and the YOLOv5s_DeepSORT mixed model was 0.9% higher in accuracy than YOLOv5s with an accuracy of 97.2% and number of frames per second: 30.

A Qualitative Research on Exploring Consideration Factors for Educational Use of ChatGPT (ChatGPT의 교육적 활용 고려 요소 탐색을 위한 질적 연구)

  • Hyeongjong Han
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.4
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    • pp.659-666
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    • 2023
  • Among the tools based on generative artificial intelligence, the possibility of using ChatGPT is being explored. However, studies that have confirmed what factors should be considered when using it educationally based on learners' actual perceptions are insufficient. Through qualitative research method, this study was to derive consideration factors when using ChatGPT in the education. The results showed that there were five key factors as follows: critical thinking on generated information, recognizing it as a tool to support learning and avoiding dependent use, conducting prior training on ethical usage, generating clear and appropriate questions, and reviewing and synthesizing answers. It is necessary to develop an instructional design model that comprehensively composes the above elements.

Early Diagnosis of anxiety Disorder Using Artificial Intelligence

  • Choi DongOun;Huan-Meng;Yun-Jeong, Kang
    • International Journal of Advanced Culture Technology
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    • v.12 no.1
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    • pp.242-248
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    • 2024
  • Contemporary societal and environmental transformations coincide with the emergence of novel mental health challenges. anxiety disorder, a chronic and highly debilitating illness, presents with diverse clinical manifestations. Epidemiological investigations indicate a global prevalence of 5%, with an additional 10% exhibiting subclinical symptoms. Notably, 9% of adolescents demonstrate clinical features. Untreated, anxiety disorder exerts profound detrimental effects on individuals, families, and the broader community. Therefore, it is very meaningful to predict anxiety disorder through machine learning algorithm analysis model. The main research content of this paper is the analysis of the prediction model of anxiety disorder by machine learning algorithms. The research purpose of machine learning algorithms is to use computers to simulate human learning activities. It is a method to locate existing knowledge, acquire new knowledge, continuously improve performance, and achieve self-improvement by learning computers. This article analyzes the relevant theories and characteristics of machine learning algorithms and integrates them into anxiety disorder prediction analysis. The final results of the study show that the AUC of the artificial neural network model is the largest, reaching 0.8255, indicating that it is better than the other two models in prediction accuracy. In terms of running time, the time of the three models is less than 1 second, which is within the acceptable range.

A Study on the Level of Perception of the Effect on Women's Health of the Artificial Abortion - Centered on Female and Male Adults - (인공유산이 여성건강에 미치는 영향에 관한 인식정도연구 - 성인 남녀 중심으로 -)

  • Kim, Sung-Hee;Yoo, Eun-Kwang
    • Women's Health Nursing
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    • v.6 no.1
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    • pp.166-180
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    • 2000
  • This study sought to find out the level of perception of the effect on women's physical, mental, socio-culture health of the artificial abortion. The research design was a descriptive correlational study and selected by convenient sampling. The subjects were 510 female and male adults who live in Seoul, Korea. Data were collected from 20th September, 1999 to 28th October, 1999. The data were analyzed through the SPSS program by use of frequency, percentage, t-test, ANOVA and Scheffe test as a post hoc contrast, $x^2$-test, Pearson correlation coefficient and stepwise multiple regression. The results of the study are as follows. 1. The subject who experienced artificial abortion was 24.3% and 468% of these had experienced repeated artificial abortion. 54.4% was using the contraceptives and there was a significant difference in the contraceptives between that women wish to use and that women are really using. 2. For the opinion on the artificial abortion, 77.3% of respondents was pro-abortionists and the most acceptable reasons of pro-choice were" In case of unmarried" and "For the women's health". 3. On the whole, the subjects perceived that artificial abortion had a negative effect on the women's health, especially, on the women's mental health. Among the items, 'guilty feeling about the baby' was the highest. 4. The related factors to the level of perception of the effect on women's health of the artificial abortion were in sex, age, occupational status, marital status, educational level, the number of children, abortion history, contraceptives women use, the frequency of the consideration on the problem of artificial abortion and the position toward abortion such as pro or anti. The main influencing factors on the level o0f perception were artificial abortion history, occupational status and the frequency of the consideration on the problem of artificial abortion. Theses factors explained 35% of total variance of the level of perception 5. The variables related to the experience of artificial abortion were age, occupational status, marital status, religion, educational level, the number of children and whether women practice contraceptives or not.

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Development and Application of a Novel Mammalian Cell Culture System for the Biocompatibility and Toxicity of Polymer Films and Metal Plate Biomaterials (고분자필름과 금속막 의료소재에 대한 생체적합성 및 독성 평가를 위한 새로운 세포배양시스템의 개발 및 적용)

  • Kwak, Moon Hwa;Yun, Woo Bin;Kim, Ji Eun;Sung, Ji Eun;Lee, Hyun Ah;Seo, Eun Ji;Nam, Gug Il;Jung, Young Jin;Hwang, Dae Youn
    • Journal of Life Science
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    • v.26 no.6
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    • pp.633-639
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    • 2016
  • Biomaterials including polymer, metal, ceramic, and composite have been widely applied for medical uses as medical fibers, artificial blood vessels, artificial joints, implants, soft tissue, and plastic surgery materials owing to their physicochemical properties. However, the biocompatibility and toxicity for film- and plate-form biomaterials is difficult to measure in mammalian cells because there is no appropriate incubation system. To solve these problems, we developed a novel mammalian cell culture system consisting of a silicone ring, top panel, and bottom panel and we applied two polymer films (PF) and one metal plate (MP). This system was based on the principal of sandwiching a test sample between the top panel and the bottom panel. Following the assembly of the culture system, SK-MEL-2 cells were seeded onto Styela Clava Tunic (SCT)-PF, NaHCO3-added SCT (SCTN)-PF, and magnesium MP (MMP) and incubated at 37℃ for 24 hr and 48 hr. An MTT assay revealed that cell viability was maintained at a normal level in the SCT-PF culture group at 24 or 48 hr, although it rapidly decreased in the SCTN-PF culture group at 48 hr. Furthermore, the cell viability in the MMP culture group was very similar to that of the control group after incubation for 24 hr and 48 hr. Together, these results suggest the sandwich-type mammalian culture system developed here has the potential for the evaluation of the biocompatibility and toxicity of cells against PF- and MP-form biomaterials.

Rapid Propagation through Tissue Culture of Cudrania tricuspidata, Medicinal Plant

  • Lee, Cheul-Ho;Min, Ji-Yun;Jung, Ha-Na;Kim, Kyu-Sick;Choi, Myung-Suk
    • Korean Journal of Medicinal Crop Science
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    • v.15 no.5
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    • pp.315-318
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    • 2007
  • An effective rapid propagation method was established through in vitro cultures of the medicinal plant, Cudrania tricuspidata. In vitro plantlets were obtained from in vitro germinated seeds. The various levels of cytokinins (BAP, Kinetin and TDZ) were tested on multiple shoot formation from plantlets. BAP (1.0 mg/l) treatment induced highest number of multiple shoots. Single shoot cultures gave higher initial shoot numbers than 5 shoots per culture. Among the various culture media, the shoot elongation was optimal on 2 MS basal medium without growth regulators. The IAA (2.0 mg/l) treatment induced highest number of roots. IBA (2.0 mg/l) treatment more promoted in vitro root growth than other concentrations. Rooted shoots were transferred directly to small pots with an artificial soil and successfully acclimatized.

Acute and Chronic Toxicity of heavy Metals to Daphnia magna (물벼룩(Daphnia magna)에 대한 중금속의 급성 및 만성 독성)

  • Jeong, Jae-Won;Cha, Mi-Seon;Jo, Sun-Ja;Lee, Sang-Jun
    • Journal of Environmental Science International
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    • v.10 no.4
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    • pp.293-298
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    • 2001
  • The toxicity values heavy metals were evaluated by immobilization and chronic reproduction impairment tests, using Daphnia magna. Acute tests were evaluated by the inhibition of their mobilization after 24hrs without food addition. The tests of reproductive impairment were investigated for 21 days by food addition and exchange or water. The effect of each concentration was assessed by Probit analysis and t-test. The results obtained from this study were as follows : 1) The change of pH and DO was not significant in the acute tests, while, in the reproductive tests, pH was increased by 0.3~1.4 and DO also increased. 2) The $E_iC_{50}$ values of immobilization to Daphnia magna in artificial fresh water were $0.030mg/{\ell}(Cu),\;0.054mg/{\ell}(Cd),\;0.12mg/{\ell};(Cr),\;0.74mg/{\ell}(Pb),\;3.4mg/ {\ell}(As)$ and the $NOE_iC$ values were $0.010mg/{\ell}(Cu),\;0.018mg/{\ell}(Cd),\;0.010mg/{\ell}(Cr),\;0.10mg/{\ell}(Pb),\;and\;$1.8mg/{\ell}(As)$. 3) The $E_rC_{50}$ values of reproductive impairment to Daphnia magna were $13.8\mu\textrm{g}/{\ell}(Cu),\;2.9\mu\textrm{g}/{\ell}(Cd),\;15.5\mu\textrm{g}/{\ell}(Cr),\;61.7\mu\textrm{g}/{\ell}(Pb),\;759\mu\textrm{g}/{\ell}(As)$, and $NOE_rC$ values were $0.95\mu\textrm{g}/{\ell}(Cu),\;$0.54\mu\textrm{g}/{\ell}(Cd),\;1.2\mu\textrm{g}/{\ell}(Cd),\;$7.4\mu\textrm{g}/{\ell}(Pb),\;110mu\textrm{g}/{\ell}(As)$. The results of tests using OECD artificial culture water were more sensitive than natural water for culturing. The presented data show that an artificial culture water is suitable in the experiment of bioassay for assessing the toxicity of marterials.

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Development of Fruitbody in the Artificial Oak Sawdust Cultures of Phellinus gilvus Mushroom (마른진흙버섯(Phellinus gilvus)의 톱밥재배 기술에 관한 연구)

  • Jo, Woo-Sik;Rew, Young-Hyun;Kim, Chang-Bae;Choi, Sung-Guk
    • The Korean Journal of Mycology
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    • v.30 no.2
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    • pp.109-112
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    • 2002
  • Present experiments were conducted to determine the possibility of artificial culture with oak sawdust block of Phellinus gilvus Mushrooms, Mycelial growth in sawdust block (oak 90 + rice bran 10, V/N) after 25 days. It took 12 days to make fruitbody from burying of sawdust block to pinhead formation. The fruitbodies produced the total fresh weight 577 g (dried weight 97 g) in a block.