• 대한한의학회지
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• 제21권1호
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• pp.53-61
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• 2000

Objectives: This study was camed out to examine the effect of five fractions of aqueous extract from Artemisia capillaris THUNB(ACT), on TGF, 1-induced apoptosis, cell viability, cell cycle progression and mRNA expression of apoptosis-related genes in human hepatocyte cell line HepG2. Methods: This study employed Tryphan blue exclusion assay, DNA fragmentation assay, Cpp32 protease activity assay and Quantitative RT-PCR analysis. Results: In the Tryphan blue exclusion assay, the butanol fraction of ACT with $TGF{\beta}$, l showed magnificent (Nice word, ut is it appropriate in a medical abstract\ulcorner) viability and the H2O fraction of ACT with $TGF{\beta}$, l also showed higher viability than only $TGF{\beta}$, l-treated group. DNA fragmentation assay showed that the butanol fraction and the H2O fraction carried inhibitory effects on apoptosis induction, with the butanol fraction displaying greater effects. The Cpp32 protease activity assay showed that the butanol fraction decreased Cpp32 protease activity. The H2O fraction of ACT had no significant effect on the Cpp32 protease activity. Quantitative RT-PCR showed that the butanol fraction suppressed Bax, p 15/INK4B, p21/Waf1, PAI-1 and increased Bcl-2 gene. Conclusions: The data shows that butanol fraction of ACT increases the hepatocyte viability and has the hepatocellular protective effect by the suppression of $TGF{\beta}$, l induced-apoptosis through gene regulation.

• 동아시아식생활학회지
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• 제19권3호
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• pp.356-362
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• 2009

The objective of this study was to investigate the biological activities of fermented mugworts and their effects on the lipid metabolism in hyperlipidemia-induced rats. The proximate compositions of two kinds of mugwort, Artemisia capillaris Thumberg and Artemisiae asiaticae Nakai, were compared before and after fermentation. In both types of mugwort crude protein and amino nitrogen contents markedly increased with fermentation. Thrombolytic activity determined from the size of the clear zone on a fibrin plate was higher for the Artemisiae asiaticae Nakai, especially in the fermented sample. Antioxidative activity according to DPPH(1,1-diphenyl-2-picrylhydrazyl) radical scavenging effects was higher for Artemisia capillaris Thumberg but no significant differences were found between the samples after fermentation. The body weights of hyperlipidemia-induced rats that were fed the mugworts for 4 weeks were lower than those of the control group. In all the mugwort-fed rats serum total cholesterol and LDL-cholesterol levels remarkably decreased. Furthermore the fermented mugworts were found to be more effective at decreasing triglyceride (TG) levels. It is also noteworthy that the highest HDL-cholesterol levels were observed in the rats treated with the fermented Artemisiae asiaticae Nakai. And fatty liver weights were higher in the rats fed Artemisia capillaris Thumberg. In conclusion the feeding of fermented Artemisiae asiaticae Nakai was effective for increasing thrombolytic activity and HDL-cholesterol levels as well as reducing TG levels in rats.

• Journal of Applied Biological Chemistry
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• 제63권4호
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• pp.335-338
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• 2020

Artemisia iwayomogi (Compositae), a perennial mugwort, is native to Korea and widely distributed in Japan, Russia, and China. A. iwayomogi and A. capillaris are similar in morphology and pharmacological activity and have been used for the same medicinal purposes in Korea. While various ingredients such as coumarins and flavonoids and their activity studies have been reported for A. capillaris, few studies have been conducted on the pharmacologically active components of A. iwayomogi. In Korea, A. capillaris is not economical because only young leaves are used as a medicinal material. Because of this, A. iwayomogi is frequently used in Korea, indicating the need to study its pharmacologically active components. Therefore, a phytochemical study was initiated to isolate active compounds from the aerial parts of A. iwayomogi. Finally, four coumarins, umbelliferone (1), esculetin (2), grevillone (3), and scoparone (4) were isolated for the first time from the aerial parts of A. iwayomogi in this study.

• 한국임상수의학회지
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• 제25권4호
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• pp.249-256
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• 2008

This experiment was conducted to investigate antitumor and immunomodulatory effects of Artemisia capillaris extracts against Hepa-lc1c7 and Sarcoma 180 cancer cells. In in vivo experimental tests using 210 ICR mice, on the $28^{th}$ day and the $42^{nd}$ day, all animals in vehicle controled HP (Hepa-lclc7 tumor cell inoculated vehicle control) and SP (Sarcoma 180 tumor cell inoculated vehicle control) showed tumor cells in the liver and spleen based on the histopathology. However, the incidences and the percentages of regions occupied by tumor cells were dramatically and dose-dependently decreased by mACH (Artemisia capillaris methanol extracts) treatment on the histomorphometry. Although the exact mechanism of inhibition of the incidences of tumor cells in the parenchyma whether inhibition of metastasis or proliferation is unclear, mACH dramatically reduce the percentages of regions occupied by tumor cells in the liver and spleen apart from the inoculation sites of Hepa-lclc7 and Sarcoma 180. In addition, they also effectively inhibit the abnormal changes on the kidney detected in the present study. The results suggest that Artemisia capillaris methanol extracts have prominent antitumor effects on the cancer cell lines Hepa-lclc7 and Sarcoma 180 m mice.

• 한국임상수의학회지
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• 제26권1호
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• pp.1-7
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• 2009

The Artemisia capillaris THUNB is a perennial herb that belongs to the family Compositae spp and probably the most common plant among the various herbal folk remedies being used in the treatment of abdominal pain, hepatitis, chronic liver disease, jaundice and coughing in Korea. Recently the biological and pharmacological actions of herb have been studied well such as antibacterial, antidiabetic and antitumor activities. This experiment was conducted to investigate antitumor and immunomodulatory effects of Artemisia capillaris extracts against Hepa-1c1c7 and Sarcoma 180 cancer cells in in vivo experimental tests. In in vivo experimental tests using 210 ICR mice, based on flow cytometry, CD3+, CD4+, CD8+ and TNF-${\alpha}+$ splenocytes were significantly (p<0.05) reduced in the Hepa-1c1c7 and Sarcoma 180 inoculated vehicle controls, HP and SP, compared to those of the intact vehicle control on both the $28^{th}$ day and the $42^{nd}$ day, respectively. These decreases of CD3+, CD4+, CD8+ and TNF-${\alpha}+$ cells induced by tumor inoculations were significantly (p<0.05) inhibited by mACH treatment regardless of the type of experiments and tumor cells inoculated. The results suggest that Artemisia capillaris methanol extracts have prominent antitumor effects on the cancer cell lines Hepa-1c1c7 and Sarcoma 180.

• 한국약용작물학회지
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• 제12권1호
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• pp.36-42
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• 2004

사철쑥 추출물은 정상폐세포인 HEL299 세포주에 $0.5\;g/{\ell}$ 이하의 농도로 투여시는 정상세포 생존율을 80% 이상으로 유지시켜 정상세포에 대한 안정성이 유지되었으며, 암세포 생육억제활성은 증류수 및 에탄올 추출물 모두 $0.5\;mg/m{\ell}$ 이상의 농도에서 유방암세포주인 MCF7에 대하여 88%이상, 폐암세포주인 A549에 대하여서는 모든 추출물에서 $0.5\;mg/m{\ell}$의 농도 이상에서 65%이상의 저해를 나타내었다. 또한 간암세포주인 Hep3B에 대하여는, 에탄올 추출물이 87% 이상, 위암세포주인 AGS에 대하여 증류수 추출물이 약 85% 이상의 높은 암세포 생육억제활성을 나타내었다. 또한 정상세포독성에 대한 암세포의 억제율을 나타내는 selectivity에 있어서 각 추출물이 $0.1\;mg/m{\ell}$에서 $1.0\;mg/m{\ell}$의 농도에서 1.5 이상의 수치를 나타내었다. B와 T 세포주의 생육촉진 실험 결과 배양 기간이 증가함에 따라 세포의 생육이 촉진되었으며, 배양 5일째 $0.5\;mg/m{\ell}$의 농도에서 B세포의 생장을 최고 1.2배 이상 증가, T-cell을 $1.2{\sim}1.5$배 이상 생장을 증가시켰으며, 배양 6일째 에탄올 추출물이 IL-6를 $67\;pg/m{\ell},\;TNF-{\alpha}$를 물 추출물이 $68\;pg/m{\ell}$의 분비 하였다.

• 생명과학회지
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• 제22권7호
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• pp.958-963
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• 2012

본 연구는 항산화 및 알러지성 피부질환에 대한 효과가 있는 안전성이 확보된 새로운 천연 향장 소재 개발을 위하여 인진호(A. capillaris)를 6종의 용매($dH_2O$, ethanol, methanol, acetone, ethyl acetate and hexane)로 추출한 추출물의 항산화 및 항아토피 활성을 검증하였다. 항산화 활성 검증에서는 methanol (DPPH: 85.87%, FRAP: 1.772)과 $dH_2O$ (DPPH: 60.69%, FRAP: 3.185) 등의 극성 용매 추출물에서 우수한 항산화 활성을 나타내었으나 비극성 용매에서는 활성이 거의 없었다(ethyl acetate 41.81%, 0.407, Hexane 8.37%, 0.328). DNFB-induced atopic dermatitis mouse model에 의한 항 아토피에 대한 치유력 측정에서는 인진호 추출물 처리군은 염증 세포의 수에서 음성 대조군과 비교하여 220% 증가로 양성 대조군의 400% 보다 약 45%의 완화 효과를 보여 주었으며, 귀의 두께는 음성 대조군의 175%로 양성 대조군의 330%보다 약 47%가 감소하는 뚜렷한 증상 완화 효과를 나타내었다. 이러한 결과는 인진호 추출물의 항산화 물질이 극성 물질이며, DNFB 처치에 의한 귀의 비후반응 감소와 염증부위의 면역세포 침투현상을 감소시키는 염증반응 완화 기능성 물질을 포함하고 있을 가능성을 알 수 있었다. 이상의 결과를 바탕으로 인진호 추출물에 대한 물리적 특성, 유전자 발현과 면역학적 실험 등이 추가 된다면 새로운 향장소재나 기능성 식품의 소재로도 이용 가능할 것으로 판단된다.

• 통합자연과학논문집
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• 제4권1호
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• pp.15-22
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• 2011

In this study, liquid with carbonized artemisia was developed for the first time that can be used as food additives and outside medicinal agents by burning artemisia species such as Artemisia dubia wall, Artemisia capillaris Thunb, Artemisia montana (Nakai) Pamp), Artemisia keiskeana Miq, Leonurus Japonicus Houtt. etc. In addition, edibility of recovered liquid with carbonized artemisia was verified by comparing the contents according to each ingredient with common refined pyroligneous liquid through the analysis of 9 kinds of organic acid by the species of artemisia, methanol and ethanol. Besides, the result of antimicrobial activities on six kinds of food spoilage and food poisoning bacteria observed by paper disc method for liquid with carbonized artemisia indicated that those two liquids showed strong antimicrobial. At the concentration of 1.5 mg/disc, clear zone for S. sonnei and S. aureus, L. monocytogenes was 13~16 mm, and they showed stronger antimicrobial activities than other strain.

• 생명과학회지
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• 제24권4호
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• pp.419-427
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• 2014

인진쑥(Artemisia capillaris Thunbl)의 항산화적 특성을 조사하기 위하여 반응표면분석법에 의하여 열수추출특성을 모니터링하여 최적 추출조건을 설정하였다. 총 페놀성화합물 함량에 대한 최적조건은 $94.50^{\circ}C$, 2.06 hr 및 시료에 대한 용매비 25.03 ml/g, 전자공여능에 대한 최적조건은 $91.82^{\circ}C$, 2.90 hr 및 20.88 ml/g으로 나타났고, 아질산염 소거능(pH 1.2)에 대한 최적조건은 $97.36^{\circ}C$, 2.75 hr 및 15.19 ml/g등으로 각각 나타났다. 각 변수에 대한 회귀식을 도출하여 인진쑥의 총 페놀성화합물 함량, 전자공여능 및 아질산염 소거능(pH 1.2)에 대한 최적 추출조건을 superimposing 한 결과, 추출온도 $90{\sim}95^{\circ}C$, 추출시간 2.5~3.5 hr 및 시료에 대한 용매비 17~24 ml/g의 범위로 각각 예측되었다.

• 한국가정과학회:학술대회논문집
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• 한국가정과학회 2001년도 학술대회:전통문화의 상품화
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• pp.101-101
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• 2001