Antioxidant Characteristics of Artemisis capillaris Hot-ｗater Extract Using Response Surface Methodology

In order to examine antioxidative characteristics of Artemisia capillaris response surface methodology was used to optimize the hot water extraction process by analyzing and monitoring the extraction condition. For total phenolic compounds content, the optimal extraction temperature, time and amount of solvent per sample were $94.50^{\circ}C$, 2.06 hr and 25.03 ml/g, respectively. Also, the optimal conditions for electronic donating ability were $91.82^{\circ}C$, 2.90 hr and 20.88 ml/g, respectively. The nitrile scavenging ability (pH 1.2) was optimized using the extraction temperature of $97.36^{\circ}C$, extraction time 2.75 hr and 15.19 ml/g as the amount of solvent per sample. Regression equations of total phenolic compounds content, electron donating ability and nitrile scavenging ability as dependent variable were deduced from each analyzed extraction condition. And finally, their response surfaces were superimposed with the optimal conditions to obtain values for each extraction process factor. The predicted results through superimposing were extraction temperature $90{\sim}95^{\circ}C$, extraction time 2.5~3.5 hr and amount of solvent per sample 17~24 ml/g.

반응표면분석법을 이용한 인진쑥 열수추출물의 항산화적 특성

인진쑥(Artemisia capillaris Thunbl)의 항산화적 특성을 조사하기 위하여 반응표면분석법에 의하여 열수추출특성을 모니터링하여 최적 추출조건을 설정하였다. 총 페놀성화합물 함량에 대한 최적조건은 $94.50^{\circ}C$, 2.06 hr 및 시료에 대한 용매비 25.03 ml/g, 전자공여능에 대한 최적조건은 $91.82^{\circ}C$, 2.90 hr 및 20.88 ml/g으로 나타났고, 아질산염 소거능(pH 1.2)에 대한 최적조건은 $97.36^{\circ}C$, 2.75 hr 및 15.19 ml/g등으로 각각 나타났다. 각 변수에 대한 회귀식을 도출하여 인진쑥의 총 페놀성화합물 함량, 전자공여능 및 아질산염 소거능(pH 1.2)에 대한 최적 추출조건을 superimposing 한 결과, 추출온도 $90{\sim}95^{\circ}C$, 추출시간 2.5~3.5 hr 및 시료에 대한 용매비 17~24 ml/g의 범위로 각각 예측되었다.