• Journal of Veterinary Clinics
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• v.19 no.2
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• pp.125-131
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• 2002

The present experiment was performed in order to know the therapeutic effect of laserpuncture and aquapuncture with methionine on recovery in artificially induced hepatic damaged rats by carbon tetrachloride. The animals were divided into control, laserpuncture and aquapuncture groups. The changes of serum enzyme activities(ALT and AST), serum total protein contents, protein fractions(ALB and GLB) and A/G ratio were examined before and after application of laserpuncture and aquapuncture. In change of serum ALT activity, recovery with treatment in laser and aquapuncture groups was more rapid than that of control. The significant decreased value was shown on 3rd(P < 0.05) and 7th day(P < 0.01) after treatment in laserpuncture group and on 7th day(P < 0.05) after treatment in aquapuncture group. In addition, significance was detected on 2nd day(P < 0.05) between laserpuncture and aquapuncture groups. In change of serum AST activity, recovery with treatment in laserpuncture and aquapuncture groups was more rapid than that of control. The significant lower values on 7th day(P<0.01) in aquapuncture groups. In addition, significant low value was detected on 7th day(P < 0.05) in aquapuncture group comparing with that of laserpuncture group. The change of serum TP contents showed similar pattern in control, laser and aquapuncture groups. The significances were detected on 7th day(P < 0.05) in both experimental groups. In addition, significant high value was detected on 2nd day(P < 0.05) in laserpuncture group comparing with that of aquapuncture group. The change of serum ALB content with treatment in laser and aquapuncture groups was more rapid than that of control. The significant high value was shown on 2nd(P < 0.01), 5th(P < 0.05) and on 7th day(P < 0.01) in laserpuncture group. In addition, significant high value was detected on 3rd day(P<0.01) only in aquapuncture group comparing with laserpuncture group. The change of serum GLB content showed similar pattern among groups. The significant low values were detected on 2nd day(P < 0.05) in aquapuncture group. In addition significant high value was detected on 5th day(P < 0.01) only in laserpuncture group comparing with that of aquapuncture group. In change of A/G ratio laser and aquapuncture groups showed higher values than that of control. The significant high values were detected on 1st day(P < 0.Of) and 2nd day(P < 0.05) in laserpuncture group, and on 1st day(P < 0.05) and 2nd day(P < 0.01) in aquapuncture group. In addition, significant high value was detected on 5th day(P < 0.05) in aquapuncture group comparing with that of laserpuncture. Considering above findings collectively, it was considered that both laserpuncture and aquapuncture were effective; aquapuncture was more effective than laserpuncture for recovery of hepatic damage.

• Journal of Veterinary Clinics
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• v.19 no.2
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• pp.115-120
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• 2002

To clarify the therapeutic effect of aquapuncture therapy on bovine foot rot, the experimental cattle were divided into control (conventional treatment), aquapuncture and combined treatment groups. Each group was composed of 6 Holstein cows with bovine foot rot. In the present study, the change in grade of lameness, total leukocyte counts, N/L. ratio, mean recovery rates (days) and histopathological changes of interdigital tissue before and after treatment were evaluated. The grade of lameness was decreased by treatment in each group, with the decreasing in order of combined > aquapuncture > control group. In change of total peripheral blood leukocyte counts, the tendency of decrease was found. Significances were detected on 9th day (P < 0.05) in control, on 6th day (P < 0.01) in aquapuncture group, and on 3rd (P < 0.05) and 6th day (P < 0.05) in combined group, respectively. In addition, significances were detected on 3rd and 6th day in aquapuncture, and on 3rd day in combined group by comparison with that of control, respectively. N/L ratio was decreased by therapy in each group with the decreasing in order of combined > aquapuncture ) control group. Significances were detected on 6th day (P < 0.01) between aquapuncture and combined groups. The mean recovery rates(days) were rapid with the decreasing in order of combined > acuapuncture > control group. In histopathological findings of interdigital tissue, the findings of inflammation and hemorrhage were not observed after treatment in each group. In conclusion, it was thought that aquapuncture and combined therapy was very effective on treatment of bovine foot rot.

• Journal of Pharmacopuncture
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• v.3 no.2
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• pp.131-152
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• 2000

The effects of Cervus elaphus aquapuncuture and Ginseng Radix aquapuncuture on the growth, protein efficiency ratio, contents of total protein, total cholesterol, and triglyceride in serum were studied for thirty four days. The results were summarized as follows. 1. Weight significantly increased in $0.3\%$ Cervus elaphus aquapuncture group on Chunchu loci and in $0.2\%$ Ginseng Radix aquapuncture group on Chunchu loci compared to normal saline aquapuncture group on Chunchu loci. 2. Protein efficiency ratio significantly increased in $0.2\%$ and $0.3\%$ of Cervus elaphus aquapuncture group on Chunchu loci and $0.1\%$, $0.2\%$ and $0.3\%$ of Ginseng Radix aquapuncture group on Chunchu loci compared to nonnal saline aquapuncture on Chunchu loci. 3. Total protein significantly increased in $0.2\%$ and $0.3\%$ of Cervus elaphus aquapuncture group on Chunchu loci compared to nonnal saline aqriapuncture On Chunchu loci. whereas it had a tendency to increase in $0.2\%$ Ginseng Radix aquapuncture group on Chunchu loci compared to normal saline aquapuncture group on Chunchu loci. 4. Total cholesterol increased a little in $0.3\%$ Cervus elaphus aquapuncture group on Chunchu loci compared to normal saline aquapuncture group on Chunchu loci. 5. Triglyceride significantly increased in $0.2\%$ Ginseng Radix aqua puncture group on Chunchu loci compared to normal saline aquapuncture group on Chunchu loci. Significance was acknowledged in the effect by density difference of medicines. In the Cervus elaphus group, there was the most significant increase in density of $0.3\%$ aquapuncture, whereas in Ginseng Radix group, there was the most significant increase in density of $0.2\%$ aquapuncture. So setting up density of medicines is important and many various studies for clinical application are required.

• Journal of Pharmacopuncture
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• v.3 no.1
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• pp.53-63
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• 2000

Carthami tinctorii Fructus is known for its good effect on diseases such as fracture, menorrhalgia, menoschesis, puerperium aneilema and so on. The study of irritation and toxicity of Carthami oil aquapuncture solution applied topically to the skin and the eye mucous membrane were carried out to prove the safety of Carthami oil aquapuncture solution in clinical use. Animal for the research was the rabbit and the solution for the test was made from Carthami semen. 6 animals were used for the skin test and 9 animals were used for the eye mucous membrane test. In results, both tests proved that Carthami oil aquapuncture solution makes no irritable reaction on skin and eye mucous membrane of rabbit. We consider that this result is helpful for saying about the safety of Carthami oil aquapuncture solution in clinical use.

• Korean Journal of Veterinary Research
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• v.45 no.2
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• pp.297-302
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• 2005

The present experiment was performed in order to know the anti-emetic effect of acupuncture, aquapuncture with metoclopramide and moxibustion on the xylazine induced emesis in dogs. The animals were devided into a control group (non-acupoint) and two experimental groups (BL-20 and LIV-13), respectively. Acupuncture, aquapuncture with metoclopramide (1 mg/kg) and moxibustion were applied to animals for 20 minutes before xylazine injection (2.2 mg/kg, IM). In acupuncture group, the emetic rates in BL-20 (16.7%) and LIV-13 (16.7%) were lower than that of control group (50%), respectively. In aquapuncture group, the emetic rates in BL-20 (16.7%) and LIV-13 (0.0%) were lower than that of control group (50%), respectively. In moxibustion group, the emetic rates in BL-20 (50%) and LIV-13 (16.7%) were lower than that of control group (83.3%), respectively. Considering above the findings collectively, it is considered that acupuncture, aquapuncture with metoclopramide and moxibustion at BL-20 and LIV-13 are effective and especially aquapuncture with metoclopramide at LIV-13 is the most effective treatment to prevent the emesis induced by xylazine among groups.

• Journal of Acupuncture Research
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• v.18 no.5
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• pp.122-134
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• 2001

The effects of Cervus elaphus and Cervus elaphus aquapuncuture on body weight, protein efficiency ratio, body length, serum growth hormone and intellectual development were studied for thirty-four days. The results were summarized as follows. 1. Body weight significantly increased in Cervus elaphus aquapuncture oral administration group compared to GH groups. 2. Protein efficiency ratio had no significant difference within all groups. 3. Body length significantly increased in Cervus elaphus aquapuncture group compared to GH injection group on 3rd day, tail length significantly increased in Cervus elaphus aquapuncture group and Cervus elaphus aquapuncture oral administration group compared to GH injection group but, body length has no significant difference within all groups. 4. Serum GH significantly increased in Cervus elaphus aquapuncture oral administration group compared to that of GH injection group. 5. As results of observing memory acquisition using Morris water maze system, there was no significant difference within all groups. 6. As results of observing retention using Morris water maze system, staying times significantly increased in Cervus elaphus aquapuncture oral administration group compared to that of GH injection group at Ist trial and 3rd trial. 7. As results of observing staining intensity of NADPH-d-positive neurons in tissue of hippocampal part, significant increasing of staining intensity were observed in septum and VDB of hippocampus in Cervus elaphus aquapuncture oral administration group compared to that of GH injection group. According to the above results, it is concluded that Cervus elaphus oral administration and Cervus elaphus aquapuncture on acupoint G39 showed effects on growth and intellectual development of animals.

• Korean Journal of Veterinary Research
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• v.51 no.4
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• pp.303-307
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• 2011

Awareness effect of aquapuncture with dexamethasone on GV-26 was studied in tiletamine/zolazapam anesthetized six healthy crossbred dogs using a randomized cross-over experimental study design. After anesthesia by tiletamine/zolazapam, 0.1 mg dexamethasone was injected on GV-26 at 20 min in the experimental group. For the control group, 0.1 mg dexamethasone was injected on the quadriceps femoralis. A significant difference was evident in the sedation score between groups at 60 and 90 min (p < 0.05). A significant difference was also evident in the analgesia score at 50 and 60 min (p < 0.05). At 90 min, all dogs in the experimental group responded strongly to pain (p < 0.01). Head up time, sternal recumbent time, standing time, and walking time was shorter in the experimental group, but the difference from the control group was statistically significant only for head up time and walking time (p < 0.05). GV-26 acupuncture with dexamethasone is useful for awareness effects after anesthesia.

• Journal of Veterinary Clinics
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• v.25 no.6
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• pp.471-475
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• 2008

The present study was performed to elucidate the therapeutic effect by aquapuncture with bee-venom (apitoxin) for treatment of canine pyoderma. The total 8 dogs with pyoderma were divided into 2 groups as control (antibiotics group: 4 dogs) and experimental groups (apitoxin group: 4 dogs). Antibiotics group was treated with bathing and antibiotics, while apitoxin group was treated with bathing and aquapuncture with apitoxin at Fei Shu (BL13), Wei Zhong (BL40), Da Zhui (GV14), Zu San Li (ST36), He Gu (LI04), Qu Chi (LI11), and San Yin Jiao (SP06). Based on the results of changes in clinical symptoms, total leukocyte counts, neutrophil/lymphocyte ratios and histopathological findings, antibiotics and apitoxin groups were all effective for treatment of canine pyoderma, and significant differences were not detected between the groups. It was suggested that apitoxin might be used as an alternative method to antibiotics for treatment of canine pyoderma.

• Journal of Veterinary Clinics
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• v.14 no.2
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• pp.308-318
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• 1997

The present experiment was performed in order to know the treatment effect of aquapuncture with hepatonics on recovery in artificially induced hepatic damaged dogs by carbon tetrachloride. The animals were divided into a control and two experimental groups (aquapuncture with taurine into Gan-su acupoint: Aa-I group and aquapuncture with taurine into blank acupoint in the thigh: Aa-II group). The changes of serum enzyme activities (ALT, AST and ALP), serum total protein contents, protein fractions and pathohistological findings of the liver were examined after application of aquapunctiuf treauent The results obtained through this experiment were summarized as follows : The serum ALT activities tendeded to decrease in experimental group compared with those of control group. Significances were detected at 5th (p<0.05) and 7th (p<0.05) day in Aa-I group and 7th day (p<0.05) in Aa-II group, respectively. Low value was fecund in Aa-I group compared with Aa-II groups but significance was not observed between two experimental groups. The serum AST activities in experimental group showed decreasing tendency compared with those of control group. Significances were observed at 2nd (p<0.05) and 5th (p<0.05) day in Aa-I group and 2nd (p<0.05) day in Aa-II groups respectively. Aa-I group showed lower values than those of Aa-II groups however, no significance was detected between experimental groups. The serum ALP activities of experimental group showed a slight decrease compared with those of control group, however, significance was not detected among all groups. The serum total protein content in experimental group showed tendency of increase compared with control group. Significance was found at 2nd day (p<0.05) in Aa-I group, but there was no significance in Aa-II group. Further significant increase of total protein content was seen at 1st day (p<0.05) in Aa-I group compared with Aa-II group. The change of serum albumin content in experimental group showed tendency of increase compared with control. Significant increases were detected at 1st (p<0.01) and 2nd (p<0.01) day in Aa-I group, respectively. Aa-II group showed increase compared with control groups but significance was not observed. Further significant increase was at 1st day (p <0.05) in Aa-I group compared with Aa-II group. The change of ${\beta} $-globulin in Aa-I group was slightly decreased compared with control group. Aa-II group was similar to controls but significance was not observed among all groups. The change of P-globulin content in Aa-I group showed tendency of increase compared with control and Aa-II group showed the tendency of decrease compared with control. The change of ${\gamma}$-globulin content experimental group showed tendency of increase compared with control, however, significance was not detected among groups. The change of A/G ratio in Aa-I group showed tendency of increase compared with control group and Aa-II group was similar to controls but no significance was found among groups. As for pathohistological observations the grade of hepatocellular vacuolized degeneration and necrosis in Aa-I group was milder than those of control and Aa-II groups and the change of Aa-II group was similar to that of control. Considering above finding collectively, it was thought that aquapuncture of Gan-su acupoint with hepatonics was more effective than aquapuncture of blank acupoint for the recovery of hepatic damage.

• Journal of Veterinary Clinics
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• v.18 no.4
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• pp.368-373
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• 2001

To study the immunomodulatory effect of aquapuncture with canine parvovirus killed vaccine, the vaccine was inoculated into the dogs twice with on 2-week interval. The 6 dogs in the experimental group were inoculated through the Jiao-Chao acupoint, and 5 dogs in the control group were done subcutaneously. The antibody titer was determined by the hemagglutination inhibition test. The HI titers of the experimental group showed significantly higher on days 21 and 28 than those of the control group. The biochemical test on serum total protein, protein fractions and the A/G ration showed a slightly increased in $\gamma$-globulin on days 21 and 28. The hematological findings on total leukocytes and differential counts showed no significance. It was thought that the aquapuncture of the canine parvovirus killed vaccine through the Jiao-Cho acupoint may stimulate the antibody production.