• Title/Summary/Keyword: antioxidant activities : phenolics

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Changes of Antioxidant Activity in Juglans mandshrica Maxim. Leaves by Far Infrared Ray Irradiation (원적외선 조사에 따른 가래나무 잎의 항산화 활성 변화)

  • Eom, Seok-Hyun;Park, Hyoung-Jae;Jin, Cheng-Wu;Park, Seung-Moon;Kim, Myong-Jo;Yu, Chang-Yeon;Cho, Dong-Ha
    • Korean Journal of Medicinal Crop Science
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    • v.15 no.4
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    • pp.266-270
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    • 2007
  • This research was conducted to investigate antioxidant activities of Juglans mandshrica forages by the irradiation of far infrared ray during leaf drying processes before compound extraction, with its potential use for activating antioxidants. The contents of total phenolics and flavonoids in the basis of a gram leaf dry weight were maximized in 20 min at 30 V of far infrared irradiation. Total phenolic content was 25.43 mg/g in the FIR treatment, while it was 21.12 mg/g in the non-FIR treatment control. Total flavonoid content was 39.38 mg/g in the FIR treatment, while it was 32.78 mg/g in the non-FIR treatment control. The contents were decreased when the condition of far infrared ray irradiation was higher voltage and longer treatment time, exhibiting 16.93 mg/g of total phenolics and 23.78 mg/g of total flavonoids in 30 min at 50 V of far infrared irradiation. The antioxidant activities were shown to positive relationships with the contents of total phenolics and total flavonoids. In this study, we concluded that content of bioactive molecules relating antioxidant activity in Juglans mandshrica forages is optimized in 20 min at 30 V of far infrared irradiation for leaf drying process.

Enhancement of Antioxidant Activities of Curcuma longa Leaves by Ultra High Pressure Extraction (초고압 공정을 이용한 강황 잎 추출물의 항산화 활성 증진)

  • Choi, Woon Yong;Lee, Hyeon Yong
    • Korean Journal of Medicinal Crop Science
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    • v.22 no.2
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    • pp.121-126
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    • 2014
  • This study was designed to evaluate antioxidant activity of Curcuma longa L. leaves treated by ultra high pressure extraction. Curcuma longa L. leaves was subjected to 5,000 bar for 5 and 15 min at $25^{\circ}C$ The highest phenolics and flavonoids content was observed in the treatment of high pressure extraction for 15 min ($308.28{\mu}g/mg$, $124.33{\mu}g/mg$). The DPPH scavenging activity was 82.34% at $1.0mg/m{\ell}$ of Curcuma longa L. leaves treated by ultra high pressure process for 15 min. The highest SOD-like acitivity of Curcuma longa L. leaves ($1.0mg/m{\ell}$) was observed at ultra high pressure extraction for 15 min (67.54%). The high pressure extraction significantly increased the contents of phenolics and flavonoids and also enhanced the antioxidant activity. These results provide useful information for enhancing biological properties of Curcuma longa L. leaves.

Biological activities of Aster scaber extracts (참취(Aster scaber) 추출물의 생리활성)

  • Lee, Eun-Ho;Park, HyeJin;Kim, Na-Hyun;Hong, Eun-Jin;Park, Mi-Jung;Lee, Seon-Ho;Kim, Myung-Uk;An, Bong-Jeun;Cho, Young-Je
    • Food Science and Preservation
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    • v.23 no.3
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    • pp.393-401
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    • 2016
  • The phenolic compounds extracted from Aster scaber were examined for their biological activities owing to their potential use in health and beauty food products. The phenolic content in water and 60% ethanol extracts were $11.1{\pm}0.11$ and $4.18{\pm}0.05mg/g$, respectively. The DPPH radical scavenging activities of the water and ethanol extracts were 87% and 91% at $50{\mu}g$ phenolics/mL, respectively. At the same phenolics concentration, the respective extracts showed 84% and 95% for ABTS radical decolorization activities and 95% and 97% for TBARs. The antioxidant protection factors for the water and ethanol extracts at $200{\mu}g$ phenolics/mL were 1.87 and 2.22 PF, respectively. Enzyme inhibitory activities of the water and ethanol extracts ($50{\mu}g$ phenolics/mL) were 50.8% and 69.4% on angiotensin converting enzyme, 91% and 80% on xanthine oxidase, and 24% and 89% on ${\alpha}$-amylase, respectively. The tyrosinase inhibitory activities indicating skin-whitening were 47% and 25% for the water and ethanol extracts, respectively. Anti-wrinkle effect of the water extract was relatively higher than that of the ethanol extract. These results suggest that the water and ethanol extracts of Aster scaber can be used as an ingredient in health and beauty food products.

Phenolic compounds and antioxidant activity of berries produced in South Korea

  • Lee, Yongcheol;Lee, Jea-Kyoo;Kim, Jeong-Gon;Park, So-Hyun;Kim, Young-Eun;Park, Sung-Kyu;Kim, Moo-Sang
    • Journal of Applied Biological Chemistry
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    • v.63 no.4
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    • pp.297-303
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    • 2020
  • Berries are rich sources of phenolic compounds, which are known to have health-promoting effects. In this study, phenolic compounds of seven popularly consumed berries were analyzed by HPLC-ESI-MS/MS. In addition, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and nitrite scavenging activities were investigated for comparison of the biological activity of the berry extracts. Chlorogenic acid was the major phenolic acid, which had the highest content in aronia, followed by blueberry. The rutin and isoquercetin contents were the highest in mulberry, followed by aronia, black raspberry, and blueberry. The anthocyanin content was the highest in black raspberry. Aronia showed the highest DPPH free radical and nitrite scavenging activities with the highest contents of total phenolics and proanthocyanidins.

Total Polyphenol, Flavonoid Contents and Antioxidant Activities of Dolsan Leaf Mustard Pickle during Storage (저장 기간 중 돌산갓 피클의 폴리페놀, 플라보노이드 및 항산화 활성에 대한 변화)

  • Son, Hae Reon;Oh, Sun Kyung;Choi, Myeong Rak
    • KSBB Journal
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    • v.31 no.2
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    • pp.100-104
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    • 2016
  • Dolsan leaf mustard has various biological functions, including those in the immune system and cancer prevention. It contains high amounts of nutritional and medicinal compounds, which are important for maintaining optimum health. The objective of this study was to determine the total phenolics contents and antioxidant activity in Dolsan leaf mustard pickle (DLMP) during storage. DLMP methanol extracts had the highest total polyphenol and flavonoid contents of $35.56{\pm}0.01GAEmg/g$ and $4.54{\pm}0.03QEmg/g$, respectively. The DPPH and ABTS radical scavenging activities in the DLMP methanol extracts showed the highest activities of 79.4 and 85.36%, respectively. The ferric reducing antioxidant power (FRAP) assay showed 2.24-3.82 mM $FeSO_4$ eq. (p<0.05) in DLMP extracts. Overall, storage at day 14 showed the highest antioxidant activity.

Antioxidant Properties of Ginseng (P. ginseng C.A. Meyer) Extracts by Organic Solvent Fractionation

  • Kim, Ji-Sang;Moon, Gap-Soon;Kim, Hyun-Oh;Lee, Young-Soon
    • Preventive Nutrition and Food Science
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    • v.12 no.4
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    • pp.267-272
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    • 2007
  • The objective of this study was to investigate antioxidant activities of Panax ginseng extracted with various solvents including n-hexane, chloroform, EtOAC, n-butanol and water. Among the various ginseng extracts, ethyl acetate (EtOAC) extracts showed the most powerful scavenging activities against DPPH radicals. Among the other solvent extracts, the butanol extract seemed relatively more effective in scavenging activity, followed by chloroform, water and hexane extracts. Moreover, the highest reducing power and ferrous ion chelating activity were found in the EtOAC extract followed by other extracts of ginseng. EtOAC extracts, which exhibited the best antioxidant activities of all solvent extracts of ginseng, possessed higher concentrations of total phenolics (777.61 mg/100 g) than other extracts. These results suggest that EtOAC extracts of ginseng (P. ginseng C.A. Meyer) have the most effective antioxidant capacity compared to n-hexane, chloroform, n-butanol and water tested in this study, and has important applications for the pharmaceutical and food industries.

Total flavonoids and phenolics in fermented soy products and their effects on antioxidant activities determined by different assays. (발효 대두 식품의 폴리페놀, 플라보노이드 함량과 다양한 측정 방법에 따른 항산화 활성 비교)

  • Park, Jeong-Woo;Lee, Young-Jin;Yoon, Sun
    • Journal of the Korean Society of Food Culture
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    • v.22 no.3
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    • pp.353-358
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    • 2007
  • This study was conducted to compare the antioxidant activities of fermented soy products such as chungkukjang, meju and doenjang with soybeans. Total phenolic, flavonoids contents and antioxidative activities of the methanol extract of soy and fermented products were compared with specific reference to alpha-diphenyl-2-picryl-hydrozyl (DPPH) radicals scavenging effects, ferric /reducing power (FRAP), inhibition of conjugated diene formation and tyrosinase activities. Total phenolic and flavonoids contents increased in soybeans after fermentation. Fermented soy products also displayed enhanced antioxidative activities in comparison with the non-fermented soybeans. There were linear relationships between total phenolic and flavonoids contents and ferric reducing/antioxidant power of the samples. Overall, the fermented soy products showed a better antioxidant activity than soybeans. Among the 3 kinds of fermented soy products, chungkukjang, meju and deonjang, doenjang exhibited the highest levels of DPPH-free radicals scavenging activity, ferric /reducing power, inhibition of conjugated diene formation and tyrosinase activities. The fermented soy products with longer fermentation time demonstrated the higher antioxidant activities as well as higher total phenols and flavonoids in the order of doenjang > meju > chunghlkjang> soybeans.

In vitro Antioxidant Activities of Trianthema portulacastrum L. Hydrolysates

  • Yaqoob, Sadaf;Sultana, Bushra;Mushtaq, Muhammad
    • Preventive Nutrition and Food Science
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    • v.19 no.1
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    • pp.27-33
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    • 2014
  • Hydrolysates of Trianthema portulacastrum in acidified methanol were evaluated for their total phenolic (TP) constituents and respective antioxidant activities using in vitro assays (i.e., 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, percent inhibition of linoleic acid peroxidation, and ferric reducing power). The observed results indicate that root, shoot, and leaf fractions of T. portulacastrum contain 50.75~98.09 mg gallic acid equivalents/g dry weight of TP. In addition, these fractions have substantial reducing potentials (0.10~0.59), abilities to inhibit peroxidation (43.26~89.98%), and DPPH radical scavenging capabilities ($6.98{\sim}311.61{\mu}g/mL$ $IC_{50}$). The experimental data not only reveal T. portulacastrum as potential source of valuable antioxidants, but also indicate that acidified methanol may be an ideal choice for the enhanced recovery of phenolic compounds with retained biological potential for the food and pharmaceutical industry.

Antioxidant Properties and Total Phenolic Contents of Cherry Elaeagnus (Elaeagnus multiflora Thunb.) Leaf Extracts

  • Shin, Seung-Ryeul;Hong, Ju-Yeon;Yoon, Kyung-Young
    • Food Science and Biotechnology
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    • v.17 no.3
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    • pp.608-612
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    • 2008
  • In Korea and China, cherry elaeagnus (Elaeagnus multiflora Thunb.) has been used traditionally to treat cough, diarrhea, itching, and foul sores. Therefore, in this study, the ethanol and water extracts of cherry elaeagnus leaves were examined for their antioxidant activities. The ethanol extract of the cherry elaeagnus leaves contained more phenolics than the water extract. All the cherry elaeagnus leaf extracts had higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability than ascorbic acid at concentrations of $250-1,000\;{\mu}g/mL$. The ethanol extract also showed higher superoxide dismutase (SOD)-like activity compared to the water extract. Furthermore, the SOD-like activity of the ethanol extract amounted to 89% of that of ascorbic acid at a concentration of $500\;{\mu}g/mL$. The nitrite scavenging ability and xanthine oxidase inhibitory (XOI) activity of the ethanol extract were higher than those of the water extract. In particular, the ethanol extract had higher XOI activity than ascorbic acid at a concentration of $1,000\;{\mu}g/mL$.

Comparative Composition of Orgainc and Inorgainc Arabica Beans and Their Antioxidant Characteristics (아라비카 원두의 산지 및 수확방법에 따른 이화학적 특성 및 항산화성)

  • Ji, Eung-Jung;Yoo, Kyung-Mi;Hwang, In-Kyeong
    • Korean journal of food and cookery science
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    • v.25 no.4
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    • pp.421-426
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    • 2009
  • The objective of this study was to evaluate the antioxidant and physiological The objective of this study was to evaluate the antioxidant and physiological activities of organic and inorganic coffee based on flavonoid composition. Organic and inorganic coffee varieties were prepared with Brazil arabica bean and Columbia arabica bean, respectively. Color values (Lightness, redness, and yellowness), mineral contents, total phenol content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, individual flavonoids, and sensory characteristics were then measured in the espresso of each coffee bean. The pH, Brix, and color difference between organic coffee and inorgainc coffee did not correlate with the harvest method. Coffee varieties were more influential on physiological properties, and were markedly more influential than the mineral content. Total phenolics had a higher correlation with DPPH radical scavenging activity ($r^2$=0.832). Depending on the harvesting method, significant differences (p<0.05) were shown in aroma, color, and overall acceptability in sensory evaluation.