• Journal of Wetlands Research
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• v.9 no.1
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• pp.91-98
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• 2007

Antifungal activity of compost teas was evaluated in vitro and in vivo experiments. In vitro test, fourteen compost teas were produced using five different composts and through six different methods for compost tea production. Eleven pathogenic fungi were used as indicators of antifungal activity of compost teas. In vivo test, one of the compost teas used in vitro test was sprayed to cucumber leaves which were infected with powdery mildew in order to evaluate antifungal activity of compost tea. From the results of the tests, it was found that all compost teas used this study showed antifungal activity and the proper application of compost teas against pathogenic fungi for obtaining optimal effects was important to know.

• Journal of Life Science
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• v.20 no.9
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• pp.1339-1344
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• 2010

In order to produce environmental-friendly fermented compost, a cattle manure-sawdust compost (antifungal compost) was developed by inoculation of B. licheniformis KJ-9 to cattle manure-sawdust. The thermal stability of the antifungal substance produced by B. licheniformis KJ-9 maintained more than 60% antifungal activity with heat treatment at $100^{\circ}C$ for 10 min, and the optimum pH of antifungal activity of the substance was 7.0. In a pot experiment with red pepper, the antifungal compost increased 1.5~2 times in leaf number and stem and root growth rate compared to those of commercial compost. Also, the diameter of stems increased 1.5-3 times in the antifungal compost treated group. The amount of microbes increased markedly in soil supplemented with antifungal compost compared to the control. In the field experiment for cultivation of garlic and Perilla japonica, the growth of both crops was significantly enhanced in the field treated with antifungal compost as compared to the commercial compost.

• Journal of Korean Society on Water Environment
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• v.24 no.3
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• pp.280-284
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• 2008

Efficacy of antifungal activity on plant pathogens by compost teas fermented under different temperatures was studied. Compost teas are recently chosen by agricultural producers for the better method of controlling plant diseases under increase of public consciousness against use of chemicals for controlling the diseases. Compost tea has been intensively studied; however, understanding of compost tea is still not well developed, and temperature influence during fermentation of compost tea on its antifungal activity has not been investigated. In this study, antifungal activities of compost teas fermented at 10, 20, 30, and $40^{\circ}C$ against selected 10 pathogens were observed. From the results, antifungal activities of compost teas at 20 and $30^{\circ}C$ of fermentation-temperatures showed the strongest while the weakest activity was observed with the compost tea at $10^{\circ}C$. Change of the activity by the fermentation-temperature apparently implied that microbes in the compost tea were strongly involved in its antifungal activity.

• Korean Journal of Organic Agriculture
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• v.6 no.1
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• pp.151-160
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• 1997

Azospirillum sp., photosynthetic bacteria(Rhodopseudomonas sp.) and Pseudomonas sp. were separated and screened from soil and soilless culture, and identificated. The antifungal activities against root-infected pathogens and plant growth promoting effects of the cultured solution of the starins(5.0$\times$105 cells/$m\ell$) in the peatmoss compost on the early growth of cucumber and tomato seedling were investigated. Azospirillum sp. and Pseudomonas sp. showed a antifungal activities against Fusarium sp., Pythium sp. and Rhizoctonia sp in thed ranges of 51.0% to 72.0% on potato dextrose agar medium, however photosynthetic bacteria had not antifungal activities. When cultured solution of Azospirillum sp., photosynthetic bacterial and Pseudomonas sp. were bacterialized by mixing with peatmoss compost, early growth of cucumber and tomato in terms of plant height, number of leaves, leaf area, root length, fresh anf dry weight of leaf, stem and root were promoted, especially photosynthetic bacteria had a the best plant growth promting activities.

• Microbiology and Biotechnology Letters
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• v.31 no.4
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• pp.421-428
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• 2003

To develop the functional-compost containing antifungal substance by using antagonistic microorganisms, Spinacia oleracea L and Rhizoctonia solani Kuhn O-28 were used as a model plant and phytopathogen, respectively. Total 80 strains were isolated from the compost of various waste foods mixture processed for a year. Among them, No.15-2 strain was selected due to its highest antifungal activity against R. solani Kuhn O-28 and was identified phyno- and phylogenotypically as Burkholderia cepacia genomovar V. which is rare probability in pathogen, by 16S rDNA sequencing and specific primer pair PCR method. B. cepacia No.15-2 preferentially dominated during the compost and its cell numbers were maintained almost $${\times}$10^{13}$ cuf/g for 15 days. The morbidity caused by R. solani Kuhn O-28 in S. oleracea L cultivation was reduced to 40% by addition of B. cepacia No.15-2. In conclusion, the antifungal compost using B. cepacia No.15-2 could be applied to biocontrol of various crops blights caused by fungal pathogen.

• Journal of the Korea Organic Resources Recycling Association
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• v.24 no.4
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• pp.85-94
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• 2016

The purpose of this study was to characterize the coffee ground and its possibility to develop the antifungal activity. pH, EC, and Zn contents of the coffee ground from coffee shops were higher than those of commercial coffee ground, but there was no significant difference in $K_2O$, CaO, MgO, $Na_2O$, Mn contents. The antimicrobial activity of the water soluble extracts from the coffee shop and the commercial coffee ground were tested for six major plant pathogens, Rhizoctonia solani, Sclerotinia sclerotiorum, Fusarium oxysporum, Phytophthora capsici, Alternaria alternata, and Botrytis cinerea. The result showed that there was reliable antifungal activity against all of tested plant pathogenic fungi. The inhibition effects of coffee ground compost extract on the spore germination and zoospore formation were investigated. Water soluble extracts of the coffee ground compost mixture added with 10% sesame oil cake were significantly inhibited the growth of conidia germination of A. altanata and zoospore formation of P. capsici in vitro. For investigating the functional materials of coffee ground compost, it was measured the total polyphenolic compounds contents with 30 days interval during decomposing coffee ground for 90 days. The total polyphenolic content increased with decomposing periods, and it observed that the highest total polyphenolic content was $0.35{\pm}0.03mg\;GAE/g$ on the 90th day in the coffee ground compost added with 10% sesame oil cake.

• Korean Journal of Environmental Biology
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• v.18 no.4
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• pp.441-446
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• 2000

Trichoderma harzianum is an aggressive causal agent of green mold disease on mushroom cultivation. Some bacterial strains isolated, from oyster mushroom compost in Wonju, were found to have in vitro antifungal activity against Trichoderma harzianum ATCC 6385, 6504, and our isolates Trichoderma spp. Y and G. Further in vitro antifungal studies on several strains of phytopathogenic fungi showed that all of 12 phytopathogenic fungal strains were significantly inhibited by the isolated antifungal bacteria in Petri dishes. Of these, KATB 99121 showed the broadest inhibiting effect and displayed as negative coagulase, negative sulfide production and rod shape. KATB 99121 was resistant to ampicillin, chlorampenicol, and kanamycin. Identification of isolates was determined by Biolog GN system, and KATB 99121 was identified as Photobacterium logei because of 96 probability, 0.65 similarity, and 4.97 disturbance. With electron microscopy, thin section of KATB 99121 strain revealed typical rod-like shaped cell (0.6-0.8${\mu}{\textrm}{m}$$\times$1.5-2.0${\mu}{\textrm}{m}$) with prokaryotic structure and organization.

• Weed & Turfgrass Science
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• v.6 no.2
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• pp.157-164
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• 2017

Functional microorganisms decompose various organic matter by enzyme activity and suppress plant disease caused by pathogen. This study was conducted to isolate and select functional microorganisms with protein or carbohydrate degradation activities and antagonistic activity against turfgrass fungal pathogens for eco-friendly turfgrass management in golf course from compost containing livestock manure of poultry or swine. Totally 68 isolates collected from livestock manure compost strains were isolated and tested for their activities of amylase, protease and lipase and antagonistic activities against Rhizoctonia solani AG2-2, R. solani AG1-1, and Sclerotinia homoeocarpa. Among the isolates, 34 strains were selected as functional microbes showing higher activities of amylase and protease. Three isolates of ASC-14, ASC-18, and ASC-35 among the 34 strains were selected as antifungal bacterial strains repressing the above 3 turfgrass fungal pathogens. Analysis results of 16s rRNA gene sequence and phylogenic cluster indicated that ASC-14 and ASC-18 belonged to Bacillus amyloliquefaciens, while ASC-35 was B. subtilis, respectively.

• Journal of Microbiology
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• v.34 no.3
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• pp.284-288
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• 1996

Among the bacteria isolated from compost piles of cattle excretion in a pasture located at the suburbs of Chunchon city, Pseudomonas aeruginosa KMCS-1 was selected for the test of antifungal substances produced. Six fractions were separated by silica gel column chromatography, and then the antifungal activity of each fraction was assayed against Escherichia coli, Bacillus subtilis, Candida albicans, Rhizopus sp., Aspergillus nidulans, Coprinus cinereus, and Pyricularia oryzae by paper disc method. Two fractions showed significant suppressive activities against A. nidulans, C. cinereus, and P. oryzae however, their mycelial growth was not affected by neither of these fractions. Inhibitory activities of these fractions to sporulation was assayed at the concentration of 50. 25, 12. 5, and 6.25 $\mu$g/ml and the average inhibition rates against sporulation of A. nidulans, C. cinereus, and P. oryzae were 94.0, 98.3, and 77.9%, respectively. Further purification and analysis of active substances are now being conducted.

• Korean Journal of Environmental Agriculture
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• v.21 no.3
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• pp.216-222
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• 2002

In order to evaluate the antifungal activity of bacteria against plant pathogenic fungi, 8 bacteria were isolated from mushroom compost hot spring, seaweed, and forest soil and mutants from them were induced by $LD_{95}$ gamma radiation($^{60}Co$). Bacillus circulans K1, Burkholderia gladioli K4 and Bacillus subtilis YS1 showed wide antifungal spectrum against 12 kinds of plant pathogenic fungi. From the radiation sensitivity test, B. gladioli K4 was very sensitive to gamma radiation and its $D_{10}$ value was 0.11 kGy. Antifungal activities of B. circulans Kl-1004 and B. subtilis YS1-1009, which were induced by the radiation of $^{60}Co$ increased against Botryosphaeria dothidea. The mutant strains, B. subtilis YS1-1006 and B. subtilis YS1-1009 were resistant to tebuconazole and copper hydroxide. SAR535, SAR5108, and SAR5118 mutated from Streptomyces sp. SAR01 were antifungal activity deficient mutants against 5 kinds of plant pathogenic fungi compared to wild strain, so that they could be supposed to be model strains far studying antifungal mechanism. It is suggested that various functional types of mutants could be induced by gamma radiation and applied usefully.