• Journal of Applied Biological Chemistry
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• v.59 no.3
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• pp.239-242
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• 2016

A novel Nicotinoyl fused peptide, Nicotinoyl-LVH, was synthesized by solid phase peptide synthesis method, purified, and tested in cultured skin cells. Nicotinoyl-LVH enhanced the expression level of collagen mRNA and its fragments in fibroblasts. These data show that this novel Nicotinoyl peptide is a promising biomaterial in the anti-aging functional cosmetic market.

• Journal of Marine Bioscience and Biotechnology
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• v.12 no.1
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• pp.66-74
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• 2020

Marine algae contain a variety of substances, such as mycosporine-like amino acids, which can defend against UV irradiation. Among them, Porphyra-334 derived from Porphyra yezoensis is attracting attention as a novel active ingredient for anti-aging cosmetics because of its excellent anti-oxidative, anti-inflammatory, and wound-healing properties through promoting skin cell migration. In this study, a process using direct current (DC) for increasing the yield of large-scale purification of Porphyra-334 was developed. When DC was applied to obtain Porphyra-334 efficiently, the purification time was shortened by approximately 1/4 compared with the process wherein DC was not applied; moreover, the yield of purification was improved.

• Journal of Korean Medical classics
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• v.28 no.2
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• pp.47-60
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• 2015

Objectives : To investigate the anti-aging effects of Joobang(酒方) on Dongeuibogam Methods : We analyzed the effect of 27 Joobang(酒方)s on Dongeuibogam and finally extracted 11 Joobang(酒方)s, which have anti-aging effects. Results & Conclusions : 1. We finally selected 12 terms of Joobang(酒方)'s anti-aging effect on Dongeuibogam. 2. We finally selected 11 Joobang(酒方)s as anti-aging candidates. 3. Twelve terms of Joobang(酒方)'s anti-aging effect and 11 candidates with anti-aging effects were classified into five categories. 4. We finally selected 17 medicinal herbs from the 11 candidates. 5. Eleven candidate anti-aging Joobang(酒方)s and 17 medicinal herbs proposed in this study have the potential to reduce the time required for experimental and clinical research for developing novel compounds with anti-aging effects.

• Proceedings of the KAIS Fall Conference
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• 2012.05a
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• pp.235-238
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• 2012

본 연구는 Glycine max (L.) Merr.의 태좌부위를 순수 분리하여 callus 세포를 유도하고 2차 대사산물인 isoflavone류에 속하는 daidzein의 생산성을 증진시킬 수 있는 방법을 찾는데 있다. 배양된 callus를 다양한 용매를 사용하여 추출하고, 추출물을 액체 크로마토그래피로 분석해 daidzein 함량의 차이를 확인하였다. 또한 핵자기공명분광법으로 50% 에탄올 추출물 내에 daidzein이 함유되어 있음을 증명하였다.

• Proceedings of the KAIS Fall Conference
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• 2012.05a
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• pp.187-190
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• 2012

본 연구는 피토케미칼과 펩타이드의 융합된 신소재의 항노화 효능에 관한 연구이다. 대표적인 피토케미칼인 갈릭산 및 시키믹산에 식물유래 Pentapeptide인 Exorphin을 융합시켜 만든 Galloyl Exorphin 및 Shikimoyl Exorphin은 콜라겐 합성능 및 항염증 효능 등을 보여주었다. 이러한 피토케미칼 융합 펩타이드 신소재는 피토케미칼이 가진 Stability 및 Solubility를 향상시키며, 피토케미칼 및 펩타이드 소재가 가진 각각의 효능을 유지시켰다. 이러한 결과는 피토케미칼 융합 펩타이드 소재가 항노화 소재로서 화장품 포물레이션에 널리 활용될 수 있음을 의미한다.

• The Korean Journal of Health Service Management
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• v.10 no.2
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• pp.145-154
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• 2016

Objectives : Because people have increasingly more interest in their quality of life, the anti-aging industry has grown worldwide. The purpose of this study is to suggest strategies for galvanizing the anti-aging industry in Korea. Methods : To accomplish this aim, we explored the definition, classification, problem and activating strategies of the anti-aging industry through a systematic review. Results : From the results, the following suggestions are given: planning a national strategy to galvanize the anti-aging industry, establishing an anti-aging research institute, developing a business model, developing an anti-aging cluster, statistics development and an information system related to anti-aging, training personnel, and legislation. Conclusions : The anti-aging industry has the potential to reduce health expenses through early intervention of diseases due to aging and through activation of the national economy by win-win growth of the industry related to aging. Therefore, understanding the anti-aging industry as well as political support is critical.

• Proceedings of the KAIS Fall Conference
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• 2012.05a
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• pp.274-277
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• 2012

지구온난화의 폐해 중 하나인 해파리의 대량증식은 환경 생태계에 좋지 않은 영향을 미치고 있고, 또한 과도한 개체수는 원할한 처리가 곤란해 많은 문제점을 초래하고 있는 실정이다. 이러한 해파리의 생산적이고 효율적인 개선방안으로 단순 가공식품 뿐만 아니라 해파리의 추출을 통해 산업적으로 활용가능한 기능성 화장품원료로의 응용가능성도 고려해 볼 수 있을 것이다. 따라서 본 연구는 보름달물 해파리 유래 추출물의 기능성 화장품 원료로서의 안전성 및 유효성을 규명하였다. 인간피부섬유아세포에서의 세포독성, 주름 개선 효과와 관련된 procollagen 발현양의 증가 및 노화로 증가되는 SA-${\beta}$-Gal 효소의 활성을 염색을 통하여 확인하였고, 항노화 화장품 신소재로서의 사업화 가능성을 확인 할 수 있었다.

• Proceedings of the KAIS Fall Conference
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• 2011.12a
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• pp.322-325
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• 2011

본 연구는 넓은잎 딱총나무 잎으로부터 유도된 캘러스의 형태학적 특성과 효능에 관한 것이다. 딱총나무에서 유도된 캘러스 내의 세포들을 촬영한 표면주사현미경 사진에서 분열능이 높은 세포의 특성을 보여주었다. 유도된 넓은잎 딱총나무 캘러스를 생물반응기 내에서 배양시켜 얻은 딱총나무 식물세포배양 추출물은 DPPH 제거 및 $PPAR{\alpha}$ 활성 실험결과에서 자유라디컬 제거능 및 항염증 효능이 높게 나타났다. 이러한 딱총나무 캘러스 세포 배양 추출물은 화장품의 항노화 신소재로서 응용될 수 있다.

• Proceedings of the KAIS Fall Conference
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• 2012.05a
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• pp.278-281
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• 2012

본 연구는 고주파를 발생시키는 고주파기기를 이용한 조류배양시스템에 관한 것으로, 미세조류 배양시 고주파 파형을 이용하는 것이다. 미세조류 배양을 위한 생물반응기에 200KHz~400KHz 고주파 파형이 나오도록 설계되었으며, Osillograph상에서 impedence가 최소가 되는 특정 지점에서 공명을 일으킬 때, 전기적 저항은 최소가 되며, 이상적인 고주파 파형 발생으로 생물반응기내의 미세조류의 Survival과 Growth에 직접적인 영향을 미치는 것을 확인하였다. 본 연구의 고주파 파형을 이용한 조류배양 시스템 개발은 자외선 흡수능을 높이는 조류배양에 활용될 수 있을 뿐만 아니라, 미세조류세포 내의 다양한 신호전달에 관련된 특정 피토케미칼, 펩타이드, 단백질 대사 연구에 유용하게 활용될 수 있다.

• Nutrition Research and Practice
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• v.15 no.6
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• pp.686-702
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• 2021

BACKGROUND/OBJECTIVES: Schisandrae Fructus, the fruit of Schisandra chinensis Baill., has traditionally been used as a medicinal herb for the treatment of various diseases, and has proven its various pharmacological effects, including anti-inflammatory and antioxidant activities. In this study, we investigated the inhibitory effect of Schisandrae Fructus ethanol extract (SF) on inflammatory and oxidative stress in particulate matter 2.5 (PM2.5)-treated RAW 264.7 macrophages. MATERIALS/METHODS: To investigate the anti-inflammatory and antioxidant effects of SF in PM2.5-stimulated RAW 264.7 cells, the levels of pro-inflammatory mediator such as nitric oxide (NO) and prostaglandin E₂ (PGE₂), cytokines including interleukin (IL)-6 and IL-1β, and reactive oxygen species (ROS) were measured. To elucidate the mechanism underlying the effect of SF, the expression of genes involved in the generation of inflammatory factors was also investigated. We further evaluated the anti-inflammatory and antioxidant efficacy of SF against PM2.5 in the zebrafish model. RESULTS: The results indicated that SF treatment significantly inhibited the PM2.5-induced release of NO and PGE₂, which was associated with decreased inducible NO synthase and cyclooxygenase-2 expression. SF also attenuated the PM2.5-induced expression of IL-6 and IL-1β, reducing their extracellular secretion. Moreover, SF suppressed the PM2.5-mediated translocation of nuclear factor-kappa B (NF-κB) from the cytosol into nuclei and the degradation of inhibitor IκB-α, indicating that SF exhibited anti-inflammatory effects by inhibiting the NF-κB signaling pathway. In addition, SF abolished PM2.5-induced generation of ROS, similar to the pretreatment of a ROS scavenger, but not by an inhibitor of NF-κB activity. Furthermore, SF showed strong protective effects against NO and ROS production in PM2.5-treated zebrafish larvae. CONCLUSIONS: Our findings suggest that SF exerts anti-inflammatory and antioxidant effects against PM2.5 through ROS-dependent down-regulating the NF-κB signaling pathway, and that SF can be a potential functional substance to prevent PM2.5-mediated inflammatory and oxidative damage.