• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2010년도 추계 학술발표회
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• pp.1030-1038
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• 2010

In order to design and construction of the Antarctic Continental Station at the Terra Nova Bay in the East Antarctic, ground characteristics for soil samples taken from the Antarctic. A series of laboratory tests were performed to investigate the variations of the thermal conductivity, the unfrozen water content according to the temperature change of the soil taken from the Antarctic. The temperature were low down below zero.

• Journal of Microbiology and Biotechnology
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• 제23권9호
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• pp.1187-1196
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• 2013

Global warming will have far-reaching effects on our ecosystem. However, its effects on Antarctic soils have been poorly explored. To assess the effects of warming on microbial abundance and community composition, we sampled Antarctic soils from the King George Island in the Antarctic Peninsula and incubated these soils at elevated temperatures of $5^{\circ}C$ and $8^{\circ}C$ for 14 days. The reduction in total organic carbon and increase in soil respiration were attributed to the increased proliferation of Bacteria, Fungi, and Archaea. Interestingly, bacterial ammonia monooxygenase (amoA) genes were predominant over archaeal amoA, unlike in many other environments reported previously. Phylogenetic analyses of bacterial and archaeal amoA communities via clone libraries revealed that the diversity of amoA genes in Antarctic ammonia-oxidizing prokaryotic communities were temperature-insensitive. Interestingly, our data also showed that the amoA of Antarctic ammonia-oxidizing bacteria (AOB) communities differed from previously described amoA sequences of cultured isolates and clone library sequences, suggesting the presence of novel Antarctic-specific AOB communities. Denitrification-related genes were significantly reduced under warming conditions, whereas the abundance of amoA and nifH increased. Barcoded pyrosequencing of the bacterial 16S rRNA gene revealed that Proteobacteria, Acidobacteria, and Actinobacteria were the major phyla in Antarctic soils and the effect of short-term warming on the bacterial community was not apparent.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2008년도 춘계 학술발표회 초청강연 및 논문집
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• pp.894-904
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• 2008

In this study, soil samples from the Antarctic and Vladivostok, Siberia were tested in the laboratory, and specific gravity, compaction curve and grain size distribution were determined. The effect of temperature change on the thermal conductivity, unfrozen water content and compressive strength were investigated. Samples for the compressive strength test were prepared in a mold with a fixed volume to prevent swelling and the effect of temperature and water content change on the strength were compared. Results from the thermal conductivity test showed that thermal conductivity values for both soils were larger at temperatures below freezing than above freezing. The unfrozen water content dropped sharply within a temperature range of $0{\sim}-5^{\circ}C$ and then gradually decreased further up to $-20^{\circ}C$. Compressive strength test results showed various stress/deformation curves with a change in water content. Sandy soil had much larger strength than pure ice at an identical temperature, while clayey soil had a smaller strength than ice near the freezing point, but showed a larger strength at temperatures belows $-15^{\circ}C$.

• 분석과학
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• 제21권5호
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• pp.364-374
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• 2008

2000년도에 COMNAP/SCAR에 의해 극지지역의 환경모니터링에 관한 표준기술지침서인 'Antarctic Environmental Monitoring Handbook'이 발간되었다. 이 지침서에 따라 남극세종기지주변지역의 토양시료중의 중금속을 분석하였다. 시료분해방법은 고압산분해 방법을 사용하였고, 분석방법으로는 동위원소희석 유도결합플라스마질량분석법을 사용하여 토양중의 Pb, Cu, Zn 성분을 분석하였다. 분석방법의 정확성을 확인하기 위해 표준시료인 NIST 2702를 분석한 결과 인증값의 99.5~100.8% 범위내에서 일치하였다. Chelex 100 이온교환수지를 사용하여 간섭이온을 제거하기 위해 매질을 분리하였다. 측정결과 인위적오염이 예상되는 기지주변지역의 중금속의 평균농도는 각각 Pb 332.9 mg/kg, Cu 95.6 mg/kg, Zn 115.3 mg/kg로 나타났으며, 기지에서 멀리 떨어진 지역의 평균농도는 각각 Pb 28.1 mg/kg, Cu 101.8 mg/kg, Zn 115.6 mg/kg로 나타났다. Pb 농도는 기지주변지역과 멀리 떨어진 지점에서 현저한 차이를 보였다.

• 미생물학회지
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• 제28권3호
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• pp.199-203
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• 1990

남극 토양생태계 세균의 분포와 세균의 생화학적 특성을 알아보기 위해 King George섬의 세종기지(한국)와 Te-niente Jubany기지(아르헨티나) 주변의 토양 17점을 남극 하계 기간(1989년 2월) 동안 채취하여 종속영양 세균의 수오 채외 효소활성도를 측정하였다. 종속영양 세균수는 시료채취지역과 배양온도가 다른 조건에서 자란 세균의 경우에도 그 평균값이 비숫한 경향을 보이고 있으나 protease는 $4^{\circ}C$에서 자란 세균의 경우 92%로서 특히 높게 나타났다. 배양온도에 따라 매우 변화가 켰으며 산술평균값은 배양온도 $37^{\circ}C$, $25^{\circ}C$, $4^{\circ}C$에서 각각 $2.5\times 10^{4}$, $2.7\times 10^{7}$, $6.9\times 10^{5}$ CFU/$cm^{3}$soil로 나타났다

• Journal of Korean Society for Atmospheric Environment
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• 제16권E2호
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• pp.85-96
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• 2000

The concentrations of Pb, Mn, Al and Na were measured from a total of 26 snow samples collected from a 1.5-m deep snow pit in Livingston Island, South Shetland Islands, at the northern tip of the Antarctic Peninsula. Ore sampling location is great concern, because of its proximity to the southern extremity of south America, a candidate for the source regions of pollutant aerosols entering Antarctica. The mean concentrations of Pb and Mn were found to be 4.97 pg g(sup)-1 and 20.6 pgg(sup)-1, respectively. These concentrations levels are similar to those reported for recent snow at other Antarctic sites with pronounced spring maxima for both metals. Contributions form natural sources are estimated to be minor (∼16%) for Pb. For Mn, on the other hand, contribution from rock and soil dusts is found to be very important. Excess Pb over Pb from natural sources is likely to be anthropogenic, especially from South Americal. Our results show that yearly Pb fallout flux is much greater in Antarctic coastal areas than at other Antarctic locations far from the coast , indicating that the transport and deposition patterns of pollutant aerosols are not simple is Antarctica. It is also suggested that the recycling of anthropogenic Pb in seawater to the atmosphere could significantly contribute to the Pb fallout flux in the Antarctic coastal regions.

• Journal of Microbiology and Biotechnology
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• 제23권5호
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• pp.661-667
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• 2013

Lipase-producing bacterial strains were isolated from Antarctic soil samples using the tricaprylin agar plate method. Seven strains with relatively strong lipase activities were selected. All of them turned out to be Bacillus pumilus strains by the 16S rRNA gene sequence analysis. Their corresponding lipase genes were cloned, sequenced, and compared. Finally, three different Bacillus pumilus lipases (BPL1, BPL2, and BPL3) were chosen. Their amino acid sequence identities were in the range of 92-98% with the previous Bacillus pumilus lipases. Their optimum temperatures and pHs were measured to be $40^{\circ}C$ and pH 9. Lipase BPL1 and lipase BPL2 were stable up to $30^{\circ}C$, whereas lipase BPL3 was stable up to $20^{\circ}C$. Lipase BPL2 was stable within a pH range of 6-10, whereas lipase BPL1 and lipase BPL3 were stable within a pH range of 5-11, showing strong alkaline tolerance. All these lipases exhibited high hydrolytic activity toward p-nitrophenyl caprylate ($C_8$). In addition, lipase BPL1 showed high hydrolytic activity toward tributyrin, whereas lipase BPL2 and lipase BPL3 hydrolyzed tricaprylin and castor oil preferentially. These results demonstrated that the three Antarctic Bacillus lipases were alkaliphilic and had a substrate preference toward short- and medium-chain triglycerides. These Antarctic Bacillus lipases might be used in detergent and food industries.

• 미생물학회지
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• 제55권1호
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• pp.83-85
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• 2019

남극 연안 툰드라 토양에서 리그닌 분해능이 있는 Pseudomonas sp. PAMC 29040를 분리하였으며, 이후 토양 유기물의 주요 구성성분인 복합유기화합물 부식질 분해능을 확인하였다. 부식질 초기 저분자화 효소(예, dye-decolorizing peroxidase)와 부식질 유래의 다양한 저분자 분해산물들을 분해하는 효소들(예, vanillate O-demethylase)를 탐색하기 위해 PAMC 29040 게놈 염기서열을 분석하였다. 분석을 통해서 최종 확보한 효소유전자 정보는 저온환경에 서식하는 토양 세균의 부식질 분해경로 제안에 활용될 것이다.

• Journal of Ecology and Environment
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• 제43권3호
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• pp.289-296
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• 2019

We investigated the mortality and the oxidative damages of Deschampsia antarctica in response to waterlogging stress. In field, we compared the changes in the density of D. antarctica tuft at the two different sites over 3 years. The soil water content at site 2 was 6-fold higher than that of site 1, and the density of D. antarctica tuft decreased significantly by 55.4% at site 2 for 3 years, but there was no significant change at site 1. Experimental results in growth chamber showed that the $H_2O_2$ and malondialdehyde content increased under root-flooding treatment (hypoxic conditions-deficiency of $O_2$), but any significant change was not perceptible under the shoot-flooding treatment (anoxic condition-absence of $O_2$). However, total chlorophyll, soluble sugar, protein content, and phenolic compound decreased under the shoot-flooding treatment. In addition, the catalase activity increased significantly on the 1st day of flooding. These results indicate that hypoxic conditions may lead to the overproduction of reactive oxygen species, and anoxic conditions can deplete primary metabolites such as sugars and protein in the leaf tissues of D. antarctica. Under present warming trend in Antarctic Peninsula, D. antarctica tuft growing near the shoreline might more frequently experience flooding due to glacier melting and inundation of seawater, which can enhance the risk of this plant mortality.

• 한국지반환경공학회 논문집
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• 제13권12호
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• pp.5-16
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• 2012

우리나라는 세종과학기지에 이어 두 번째로 남극에 과학기지를 건설하는 사업을 진행하고 있다. 남극은 영구동토지역으로 분류되지만 일부 지반의 상부에는 활동층이 존재한다. 이 활동층이 동결되면 동결팽창으로 인해 지반의 융기가 발생하고 구조물에 부가적인 응력이 유발되어 구조물 안정에 심각한 영향을 미치게 된다. 그러므로 남극 기지 건설지에 대한 동상 특성을 파악하는 것은 구조물의 수명 및 안정성을 확보하기 위해 필수적이라고 할 수 있다. 본 연구의 목적은 남극 제2기지 건설지에 대한 동상민감성을 정량적으로 평가하고 흙 입자의 크기와 부동수분량이 동상에 미치는 영향을 파악하는 것이다. 이를 위해 동남극 테라노바만 주변 기지 건설지 표층에서 채취한 흙 시료에 대해 기본물성실험과 부동수분실험, 동상실험을 수행하였다. 동상실험의 경우 공시체 상부에 냉기를 주입하고 하부에는 물을 지속적으로 공급하여 동상이 발생하도록 유도하였다. 동상실험으로부터 공시체의 동상량과 동상비, 동상속도를 도출하였고, 실험 결과가 흙 입자의 크기 및 부동수분량과 어떤 상관관계가 있는지 분석하였다. 분석 결과 채취한 시료의 미세분 함량이 일정비율을 넘으면 흙 시료의 동상이 크게 발생하였고, 동결토 내의 부동수분에 따라 동상정수는 영향을 받는 것으로 나타났다. 본 연구는 동남극 테라노바만 흙 시료에 대하여, 동결토의 동상에 중요한 영향인자인 흙 입자의 크기 및 부동수분량과 동상량 사이의 관계를 실험적으로 규명하였다는 데에 그 의의가 있다.