Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Draft genome sequence of humic substance-degrading Pseudomonas sp. PAMC 29040 from Antarctic tundra soil

천연 복합유기화합물인 부식질을 분해하는 남극 툰드라 토양 Pseudomonas sp. PAMC 29040의 유전체 분석

  • Kim, Dockyu (Division of Polar Life Sciences, Korea Polar Research Institute) ;
  • Lee, Hyoungseok (Unit of Polar Genomics, Korea Polar Research Institute)
  • 김덕규 (극지연구소 극지생명과학연구부) ;
  • 이형석 (극지연구소 극지유전체사업단)
  • Received : 2019.01.17
  • Accepted : 2019.03.11
  • Published : 2019.03.31
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Abstract

Pseudomonas sp. PAMC 29040 was isolated from a maritime tundra soil in Antarctica for its ability to degrade lignin and subsequently confirmed to be able to depolymerize heterogeneous humic substance (HS), a main component of soil organic matter. The draft genome sequences of PAMC 29040 were analyzed to discover the putative genes for depolymerization of polymeric HS (e.g., dye-decolorizing peroxidase) and catabolic degradation of HS-derived small aromatics (e.g., vanillate O-demethylase). The information on degradative genes will be used to finally propose the HS degradation pathway(s) of soil bacteria inhabiting cold environments.

남극 연안 툰드라 토양에서 리그닌 분해능이 있는 Pseudomonas sp. PAMC 29040를 분리하였으며, 이후 토양 유기물의 주요 구성성분인 복합유기화합물 부식질 분해능을 확인하였다. 부식질 초기 저분자화 효소(예, dye-decolorizing peroxidase)와 부식질 유래의 다양한 저분자 분해산물들을 분해하는 효소들(예, vanillate O-demethylase)를 탐색하기 위해 PAMC 29040 게놈 염기서열을 분석하였다. 분석을 통해서 최종 확보한 효소유전자 정보는 저온환경에 서식하는 토양 세균의 부식질 분해경로 제안에 활용될 것이다.

Keywords

MSMHBQ_2019_v55n1_83_f0001.png 이미지

Fig. 1. Proposed HS-degradative pathway by Pseudomonas sp. PAMC 29040.

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