Journal of Ecology and Environment

The Ecological Society of Korea (한국생태학회)
권호 표지
DOI QR코드

DOI QR Code

Waterlogging induced oxidative stress and the mortality of the Antarctic plant, Deschampsia antarctica

  • Park, Jeong Soo (Alien Species Research Team, National Institute of Ecology) ;
  • Lee, Eun Ju (School of Biological Sciences, Seoul National University)
  • Received : 2019.06.11
  • Accepted : 2019.07.17
  • Published : 2019.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

We investigated the mortality and the oxidative damages of Deschampsia antarctica in response to waterlogging stress. In field, we compared the changes in the density of D. antarctica tuft at the two different sites over 3 years. The soil water content at site 2 was 6-fold higher than that of site 1, and the density of D. antarctica tuft decreased significantly by 55.4% at site 2 for 3 years, but there was no significant change at site 1. Experimental results in growth chamber showed that the $H_2O_2$ and malondialdehyde content increased under root-flooding treatment (hypoxic conditions-deficiency of $O_2$), but any significant change was not perceptible under the shoot-flooding treatment (anoxic condition-absence of $O_2$). However, total chlorophyll, soluble sugar, protein content, and phenolic compound decreased under the shoot-flooding treatment. In addition, the catalase activity increased significantly on the 1st day of flooding. These results indicate that hypoxic conditions may lead to the overproduction of reactive oxygen species, and anoxic conditions can deplete primary metabolites such as sugars and protein in the leaf tissues of D. antarctica. Under present warming trend in Antarctic Peninsula, D. antarctica tuft growing near the shoreline might more frequently experience flooding due to glacier melting and inundation of seawater, which can enhance the risk of this plant mortality.

Keywords

References

  1. Ahmed S, Nawata E, Hosokawa M, Domae Y, Sakuratani T. Alterations in photosynthesis and some antioxidant enzymatic activities of mungbean subjected to waterlogging. Plant Sci. 2002;163(1):117-23. https://doi.org/10.1016/S0168-9452(02)00080-8
  2. Ainsworth EA, Gillespie KM. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nature protocols. 2007;2(4):875. https://doi.org/10.1038/nprot.2007.102
  3. Alberdi M, Bravo LA, Gutierrez A, Gidekel M, Corcuera LJ. Ecophysiology of Antarctic vascular plants. Physiol. Plant. 2002;115(4):479-86. https://doi.org/10.1034/j.1399-3054.2002.1150401.x
  4. Allen RD. Dissection of oxidative stress tolerance using transgenic plants. Plant Physiol. 1995;107(4):1049. https://doi.org/10.1104/pp.107.4.1049
  5. Azevedo R, Alas R, Smith R, Lea P. Response of antioxidant enzymes to transfer from elevated carbon dioxide to air and ozone fumigation, in the leaves and roots of wild type and a catalase deficient mutant of barley. Physiol. Plant. 1998;104(2):280-92. https://doi.org/10.1034/j.1399-3054.1998.1040217.x
  6. Bergmeyer HU. Methods of enzymatic analysis. Weinheim: Verlag Chemie; 1974.
  7. Bravo LA, Ulloa N, Zuniga GE, Casanova A, Corcuera LJ, Alberdi M. Cold resistance in Antarctic angiosperms. Physiol Plant. 2001;111(1):55-65. https://doi.org/10.1034/j.1399-3054.2001.1110108.x
  8. Bray RH, Kurtz L. Determination of total, organic, and available forms of phosphorus in soils. Soil Sci. 1945;59(1):39-46. https://doi.org/10.1097/00010694-194501000-00006
  9. Collaku AH. Losses in wheat due to waterlogging. Crop Sci. 2002;42(2):444-50. https://doi.org/10.2135/cropsci2002.4440
  10. Davies DD. Anaerobic metabolism and the production of organic acids. In: Davies DD, editor. The Biochemistry of Plants, Vol. 2. New York: Academic Press; 1980.
  11. Day PR, Particle fractionation and particle-size analysis. In Black, C. A. (ed.), Methods of soil analysis. Part 1. Physical and Mineralogical Properties, Including Statistics of Measurement and Sampling. ASA, SSSA, 1965;9:545-567.
  12. Drew MC. Oxygen deficiency and root metabolism: injury and acclimation under hypoxia and anoxia. Ann Rev Plant Biol. 1997;48(1):223-50. https://doi.org/10.1146/annurev.arplant.48.1.223
  13. Gill SS, Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol. Biochem. 2010;48(12):909-30. https://doi.org/10.1016/j.plaphy.2010.08.016
  14. Hodges DM, Delong JM, Forney CF, Prange RK. Improving the thiobarbituric acidreactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta. 1999;207(4):604-11. https://doi.org/10.1007/s004250050524
  15. Hsu YM, Tseng MJ, Lin CH. The fluctuation of carbohydrates and nitrogen compounds in flooded wax-apple trees. Bot Bull Acad Sin. 1999;40:193-8.
  16. John B. A comparison of two methods for estimating the organic matter content of sediments. J Paleolimnol. 2004;31:125-7. https://doi.org/10.1023/B:JOPL.0000013354.67645.df
  17. Kalashnikov J, Balakhnina T, Zakrzhevsky D. Effect of soil hypoxia on activation of oxygen and the system of protection from oxidative destruction in roots and leaves of Hordeum vulgare. Russ J Plant Physiol. 1994;41:583-8.
  18. Kovar JL, Pierzynski GM. Methods of phosphorus analysis for soils, sediments, residuals, and waters. Southern cooperative series bulletin No. 408. (Second Ed.); 2009.
  19. Kruger NJ. The Bradford method for protein quantitation. In: The protein protocols handbook. Totowa: Humana Press; 2009.
  20. Lee J, Jin YK, Hong JK, Yoo HJ, Shon H. Simulation of a tidewater glacier evolution in Marian Cove, King George Island, Antarctica. Geosci J. 2008;12(1):33-9. https://doi.org/10.1007/s12303-008-0005-x
  21. Liao CT, Lin CH. Photosynthetic responses of grafted bitter melon seedlings to flooding stress. Environ Exp Bot. 1996;36:167-72. https://doi.org/10.1016/0098-8472(96)01009-X
  22. Liao CT, Lin CH. Physiological adaptation of crop plants to flooding stress. Proceedings of the National Science Council, Republic of China. Part B Life Sci. 2001;25(3):148-57.
  23. Marshall J. Drought and shade interact to cause fine-root mortality in Douglas-fir seedlings. Plant Soil. 1986;91(1):51-60. https://doi.org/10.1007/BF02181818
  24. Park JS, Ahn IY, Lee EJ. Spatial distribution patterns of the Antarctic Hair grass Deschampsia antarctica in relation to environmental variables on Barton Peninsula, King George Island. Arct Antarct Alp Res. 2013;45(4):563-74. https://doi.org/10.1657/1938-4246-45.4.563
  25. Perez-Torres E, Garcia A, Dinamarca J, Alberdi M, Gutierrez A, Gidekel M, Ivanov AG, NPA H, Corcuera LJ, Bravo LA. The role of photochemical quenching and antioxidants in photoprotection of Deschampsia antarctica. Funct Plant Biol. 2004;31(7):731-41. https://doi.org/10.1071/FP03082
  26. Pezeshki SR. Wetland plant responses to soil flooding. Environ Exp Bot. 2001;46(3):299-312. https://doi.org/10.1016/S0098-8472(01)00107-1
  27. Ricard B, Couee I, Raymond P, Saglio P, Saint-Ges V, Pradet A. Plant metabolism under hypoxia and anoxia. Plant Physiol Biochem. 1994;32:1):1-10.
  28. Sairam R, Kumutha D, Ezhilmathi K, Chinnusamy V, Meena R. Waterlogging induced oxidative stress and antioxidant enzyme activities in pigeon pea. Biol Plant. 2009;53(3):493-504. https://doi.org/10.1007/s10535-009-0090-3
  29. Sairam R, Kumutha D, Ezhilmathi K, Deshmukh P, Srivastava G. Physiology and biochemistry of waterlogging tolerance in plants. Biol Plant. 2008;52(3):401-12. https://doi.org/10.1007/s10535-008-0084-6
  30. Smith R. The enigma of Colobanthus quitensis and Deschampsia antarctica in Antarctica. Antarctic biology in a global context. Leiden: Backhuys Publishers; 2003.
  31. Vaughan DG, Marshall GJ, Connolley WM, Parkinson C, Mulvaney R, Hodgson DA, King JC, Pudsey CJ, Turner J. Recent rapid regional climate warming on the Antarctic Peninsula. Clim Change. 2003;60(3):243-74. https://doi.org/10.1023/A:1026021217991
  32. Wellburn AR. The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol. 1994;144(3):307-13. https://doi.org/10.1016/S0176-1617(11)81192-2
  33. Yamasaki H, Sakihama Y, Ikehara N. Flavonoid-peroxidase reaction as a detoxification mechanism of plant cells against $H_2O_2$. Plant Physiol. 1997;115(4):1405-12. https://doi.org/10.1104/pp.115.4.1405
  34. Yetisir H, Caliskan ME, Soylu S, Sakar M. Some physiological and growth responses of watermelon [Citrullus lanatus (Thunb.) Matsum. and Nakai] grafted onto Lagenaria siceraria to flooding. Environ Exp Bot. 2006;58:1-8. https://doi.org/10.1016/j.envexpbot.2005.06.010
  35. Zamora P, Rasmussen S, Pardo A, Prieto H, Zuniga GE. Antioxidant responses of in vitro shoots of Deschampsia antarctica to Polyethylene glycol treatment. Antarct Sci. 2010;22(2):163-9. https://doi.org/10.1017/S0954102009990733
  36. Zuniga GE, Alberdi M, Corcuera LJ. Non-structural carbohydrates in Deschampsia antarctica desv. from South Shetland Islands, maritime antarctic. Environ Exp Bot. 1996;36(4):393-9. https://doi.org/10.1016/S0098-8472(96)01026-X

Cited by

  1. Exogenous salicylic acid ameliorates waterlogging stress damages and improves photosynthetic efficiency and antioxidative defense system in waxy corn vol.59, pp.1, 2019, https://doi.org/10.32615/ps.2021.005
  2. Genotoxicity of oxidative stress and UV-B radiation in Antarctic vascular plants vol.44, pp.5, 2019, https://doi.org/10.1007/s00300-021-02860-1