• Title/Summary/Keyword: animal experiment

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Assessment of Equine Temperament by a Questionnaire Survey (Questionnaire Survey를 이용한 말의 품성 평가)

  • Kim, Nam-Young;Park, Yong-Sang;Ko, Moon-Suck;Chae, Hyun-Seok;Cho, Won-Mo;Cho, In-Chul;Hong, Hyun-Ju;Park, Ji-Yun;Chung, Kwang-Yeoun;Cho, Sang-Rae
    • Journal of Embryo Transfer
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    • v.28 no.3
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    • pp.223-227
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    • 2013
  • The objective of the study is to assess temperament of a horse based on general temperament test by a questionnaire survey. Five test criteria were identified: gentleness, patience, aggressiveness, sensitivity, and friendliness, each on a 5-point scale. 114 horses bred at the National Institute of Animal Science (NIAS) in 2010, 2011 and 2012. The horses recorded scores of 3.6~3.9 for gentleness, 3.1~3.6 for patience, 3.4~4.0 for aggressiveness, 2.8~3.2 for sensitivity, and 3.4~3.8 for friendliness, the overall score for sensitivity the lowest. Horses born in 2012 scored lower than the rest in all five areas at a statistically significant level (P<0.05). By gender, the colts scored higher than the fillies in all five areas, but the discrepancy was not statistically significant. Factor analysis yielded only one factor, and the Cronbach's ${\alpha}$ value was 0.980 for standardization of Factor 1, indicating a high reliability of internal consistency. The correlation coefficients among the test criteria ranged between 0.85 and 0.91 (P<0.01). The assessment criteria used in this study are expected to provide a useful basis designing a temperament test horses.

Hanwoo cattle: origin, domestication, breeding strategies and genomic selection

  • Lee, Seung-Hwan;Park, Byoung-Ho;Sharma, Aditi;Dang, Chang-Gwon;Lee, Seung-Soo;Choi, Tae-Jeong;Choy, Yeon-Ho;Kim, Hyeong-Cheol;Jeon, Ki-Jun;Kim, Si-Dong;Yeon, Seong-Heum;Park, Soo-Bong;Kang, Hee-Seol
    • Journal of Animal Science and Technology
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    • v.56 no.1
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    • pp.2.1-2.8
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    • 2014
  • Hanwoo (Korean cattle) is the native, taurine type of cattle breed of Korea and its history as a draft animal dates back to 5000 Years. In earlier times Hanwoo was used extensively for farming, transportation. Over the period of time, Hanwoo has changed to be meat type cattle. Full-scale production of Hanwoo as meat-type cattle has occurred since 1960s with the rapid growth of the Korean economy. Hanwoo is one of the most economically important species in Korea as it is a significant source of nutrition to the Korean people. Hanwoo beef is the most cherished food of Korea. One of the main goals of researchers is to increase the meat quality, quantity and taste of the beef. In this review we describe the origin, domestication of Hanwoo cattle and breeding program initiated from 1980's. Moreover the advent of technological advancement had provided us a platform to perform genome wide selection on economic traits and its implementation into traditional breeding programs.

Strategies to Multiply Elite Cow in Hanwoo Small Farm

  • Lee, Seung Hwan;Kim, Ui Hyung;Dang, Chang Gwan;Aditi, Sharma;Kim, Hyeong Cheul;Yeon, Seung Heum;Jeon, Gi Jun;Chang, Sun Sik;Oh, Sung Jong;Lee, Hak Kyo;Yang, Bo Suk;Kang, Hee Seol
    • Journal of Embryo Transfer
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    • v.28 no.2
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    • pp.79-85
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    • 2013
  • The recent development in genetic assisted selection (combining traditional- and genome assisted selection method) and reproduction technologies will allow multiplying elite cow in Hanwoo small farm. This review describes the new context and corresponding needs for genome assisted selection schemes and how reproductive technologies can be incorporated to get more genetic gain for cow genetic improvement in Hanwoo. New improved massive phenotypes and pedigree information are being generated from commercial farm sector and these are allowing to do genetic evaluation using BLUP to get elite cows in Korea. Moreover cattle genome information can now be incorporated into breeding program. In this context, this review will discuss about combining the reproductive techniques (Multiple Ovulation Embryo Transfer; MOET) and genome assisted selection method to get more genetic gain in Hanwoo breeding program. Finally, how these technologies can be used for multiplication of elite cow in small farm was discussed.

Efficacy of a Cue-Mate Intravaginal Insert and Injection of Prostaglandin $F_{a{\alpha}}$ for Synchronizing Estrus in Hanwoo Cattle

  • Cho, Sang-Rae;Kumar, Kuldeep;Yeon, Seong-Heum;Lee, Sung-Hwan;Kim, Ui-Hyung;Chung, Ki-Yong;Kim, Hyeon-Shup;Lee, Myeung Sik;Park, Chang-Seok;Yang, Byoung-Chul
    • Journal of Embryo Transfer
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    • v.29 no.4
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    • pp.321-325
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    • 2014
  • The present study was performed on farm animals to test the effectiveness of progesterone-releasing intravaginal device (Cue-Mate$^{(R)}$ 1.56 g) and injection of prostaglandin $F_{2{\alpha}}$ ($PGF_{2{\alpha}}$) for synchronization estrus in Hanwoo cattle. The cattle were at random stage of the estrus cycle. The cows were artificially inseminated at day 7 after Cue-Mate withdrawal, using commercial semen from Korean native bulls. There was a season effect on the estrus synchronization rate. It was higher in spring (94.3%) followed by winter (93.3%), autumn (90.4%) and summer (67.2%). In summary, The results of this study revealed that season has influences on estrus behavior of cattle with no significant effect on pregnancy rate. In summary, we suggest summer reproductive management to alleviate the effects of heat stress. It should be based on intensive cooling combined with hormonal treatment. Given that different subgroups of cows benefit differently from the treatments, selective hormonal administration should be considered.

A Parentage Test using Indel, Microsatellite Markers and Genotypes of MC1R in the Jeju Black Cattle Population (제주 흑우 집단에서 Indel, Microsatellite 마커와 MC1R 유전자형을 이용한 친자 확인)

  • Han, Sang Hyun;Cho, Sang-Rae;Cho, In-Cheol;Cho, Won-Mo;Kim, Sang-Geum;Yang, Sung-Nyun;Kang, Yong-Jun;Park, Yong-Sang;Kim, Young-Hoon;Park, Se-Phil;Kim, Eun-Young;Lee, Sung-Soo;Ko, Moon-Suck
    • Journal of Embryo Transfer
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    • v.28 no.3
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    • pp.207-213
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    • 2013
  • This study was carried out to examine a molecular marker system for parentage test in Jeju Black cattle (JBC). Based on the preliminarily studies, we finally selected for construction of a novel genetic marker system for molecular traceability, identity test, breed certification, and parentage test in JBC and its related industrial populations. The genetic marker system had eight MS markers, five indel markers, and two single nucleotide polymorphisms (SNPs; g.G299T and g.del310G) within MC1R gene which is critical to verify the breed specific genotypes for coat color of JBC differing from those of exotic black cattle breeds such as Holstein and Angus. The results showed lower level of a combined non-exclusion probability for second parent (NE-P2) of $4.1202{\times}10^{-4}$ than those previously recommended by International Society of Animal Genetics (ISAG) of $5.000{\times}10^{-4}$ for parentage, and a combined non-exclusion probability for sib identity (NE-SI) of $2.679{\times}10^{-5}$. Parentage analysis has been successfully identified the JBC offspring in the indigenous population and cattle farms used the certified AI semens for production using the JBC-derived offspring for commercial beef. This combined molecular marker system will be helpful to supply genetic information for parentage test and traceability and to develop the molecular breeding system for improvement of animal productivity in JBC population.

Genome-wide Association Study for Warner-Bratzler Shear Force and Sensory Traits in Hanwoo (Korean Cattle)

  • Dang, C.G.;Cho, S.H.;Sharma, A.;Kim, H.C.;Jeon, G.J.;Yeon, S.H.;Hong, S.K.;Park, B.Y.;Kang, H.S.;Lee, S.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.9
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    • pp.1328-1335
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    • 2014
  • Significant SNPs associated with Warner-Bratzler (WB) shear force and sensory traits were confirmed for Hanwoo beef (Korean cattle). A Bonferroni-corrected genome-wide significant association (p< $1.3{\times}10^{-6}$) was detected with only one single nucleotide polymorphism (SNP) on chromosome 5 for WB shear force. A slightly higher number of SNPs was significantly (p<0.001) associated with WB shear force than with other sensory traits. Further, 50, 25, 29, and 34 SNPs were significantly associated with WB shear force, tenderness, juiciness, and flavor likeness, respectively. The SNPs between p = 0.001 and p = 0.0001 thresholds explained 3% to 9% of the phenotypic variance, while the most significant SNPs accounted for 7% to 12% of the phenotypic variance. In conclusion, because WB shear force and sensory evaluation were moderately affected by a few loci and minimally affected by other loci, further studies are required by using a large sample size and high marker density.

Protein Patterns on a Corpus Luteum during Pregnancy in Korean Native Cows

  • Chung, Hak-Jae;You, Dong-Min;Kim, Hyo-Ju;Choi, Hye-Young;Lee, Myeong-Suk;Kim, Jin-Bum;Lee, Suck-Dong;Park, Jung-Yong;Lee, Myeung-Sik
    • Reproductive and Developmental Biology
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    • v.34 no.3
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    • pp.263-270
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    • 2010
  • Luteal cells produce progesterone that supports pregnancy. Steroidogenesis requires coordination of the anabolic and catabolic pathways of lipid metabolism. In the present study, the corpus luteum (CL) in early pregnancy established from luteal phase and pregnant phase was analyzed. The first study determined progesterone changes in the bovine CL at day 19 (early maternal recognition period) and day 90 in mid-pregnancy and compared them to the CL from day 12 of the estrous cycle. CL alternation was tested using two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF). Comparing CL from luteal phase to those from pregnant phase counterparts, significant changes in expression level were found in 23 proteins. Of these proteins 17 were not expressed in pregnant phase CL but expressed in luteal phase counterpart, whereas, the expression of the other 6 proteins was limited only in pregnant phase CL. Among these proteins, vimentin is considered to be involved in regulation of post-implantation development. In particular, vimentin may be used as marker for CL development during pregnancy because the expression level changed considerably in pregnant phase CL tissue compared with its luteal phase counterpart. Data from 2-DE suggest that protein expression was disorientated in mid pregnancy from luteal phase, but these changes was regulated with progression of pregnancy. These findings demonstrate CL development during mid-pregnancy from luteal phase and suggest that alternations of specific CL protein expression may be involved in maintenance of pregnancy.

Evaluation of the Cardiorespiratory Endurance of Jeju Crossbred Horses according to Exercise Intensity (제주산마의 운동강도별 심폐지구력 측정)

  • Kim, Nam-Young;Park, Yong-Sang;Ko, Moon-Suck;Chae, Hyun-Seok;Cho, Won-Mo;Cho, In-Chul;Hong, Hyun-Ju;Park, Ji-Yun;Cho, Sang-Rae;Kang, Tae-Young
    • Journal of Veterinary Clinics
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    • v.30 no.6
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    • pp.449-452
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    • 2013
  • The present study was conducted to measure the cardiorespiratory endurance of Jeju crossbred horses according to hill tests and exercise intensity, thereby providing foundational data for evaluation of running capability of horses. The nine Jeju crossbred horses were subjected to the hill test with an inclination of around $30^{\circ}$ and six out of nine horses were used to measure the cardiorespiratory endurance according to three different exercise intensities (moderate, heavy, and very heavy). The running time for the hill test was 205 sec on average, while high deviation was observed between horses from 167 to 258 sec. In addition, the running time for the hill test was highly correlated with moderate exercise. While no significant difference in average heart rate was found between exercise intensities, a trend of large variation was found in the average heart rate between horses. The heart rate recovery was highly correlated with heavy exercise and very heavy exercise. The results showed that no significant difference in average heart rate was found among three exercise intensities, but there are large individual variations among horses.

The bovin phylogeny: A review

  • Sharma, Aditi;Lee, SeungHwan;Lee, JunHeon;Dang, Changgwon;Kim, Hyeong Cheul;Yeon, SeongHum;Kang, HeeSeol;Kanwar, Shamsher Singh;Vijh, Ramesh Kumar
    • Korean Journal of Agricultural Science
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    • v.41 no.4
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    • pp.405-413
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    • 2014
  • The evolutionary history of cattle and buffalo has always been a topic of great interest to the evolutionary biologists. The phylogenetic studies of bovin species has been carried out at various levels, varying from the study of domestication and migration of populations to major cladogenesis. Along with the archeological studies there are studies from molecular biology and more recently from genomics. The phylogenetic perspective of the bovins and their evolutionary history, are reviewed in terms of what has been done, what needs to be done and potential challenges in doing it.

Evaluation of pig behavior changes related to temperature, relative humidity, volatile organic compounds, and illuminance

  • Kim, Yong Ju;Song, Min Ho;Lee, Sang In;Lee, Ji Hwan;Oh, Han Jin;An, Jae Woo;Chang, Se Yeon;Go, Young Bin;Park, Beom Jun;Jo, Min Seok;Lee, Chang Gyu;Kim, Hyeun Bum;Cho, Jin Ho
    • Journal of Animal Science and Technology
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    • v.63 no.4
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    • pp.790-798
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    • 2021
  • The objective of this study was evaluation of pig behavior changes related to temperature, relative humidity, volatile organic compounds (VOCs), and illuminance. A total of 24 growing pigs ([Yorkshire × Landrace] × Duroc) were used in the experiment. A sensor was installed at a height of 0.5 m in the center of the pig house. In experiment 1, temperature was changed every four days to 18℃ (T1), 22℃ (T2), 26℃ (T3), and then 30℃ (T4). In experiment 2, relative humidity was adjusted to 45% (low humidity [LH]), 60% (middle humidity [MH]), and then 75% (high humidity [HH]) for four days. In experiment 3, after cleaning the pig house just before experiment, only minimal ventilation was provided. VOCs and pig behaviors were observed for 7 days without cleaning the pig house. In experiment 4, three light bulbs of 40 W (470 lumens / 45 lx; low illuminance [LI]), 75 W (1,055 lumens / 103 lx; middle illuminance [MI]), and 100 W (1,521 lumens / 146 lx; high illuminance [HI]) were used for four days each. Pig behavior analysis was performed for following criteria : Feed intake, Standing, Lying, Sitting, Drink water, Rooting, Posture transition (lying-standing), Posture transition (standing-lying), Wallowing, and Biting. In experiment 1, feed intake time was lower (p < 0.05) for the T3 than other treatment groups. Standing time was highest (p < 0.05) for the T1 and lowest (p < 0.05) for the T3. Lying time was shorter (p < 0.05) in T1 and T2 compared to T3 and T4. Drinking frequency was higher (p < 0.05) for the T4 than other treatment groups. In experiment 2, the frequency of rooting and wallowing increased (p < 0.05) with increasing humidity. LH showed the lowest (p < 0.05) rooting frequency and HH showed the highest (p < 0.05) rooting frequency. In experiment 3, VOCs concentration did not (p > 0.05) change pig behavior. In experiment 4, lying time was the longest (p < 0.05) at LI and shortest (p < 0.05) at HI. Therefore, pig behavior is heavily influenced by the environment, especially temperature and humidity. However, correlation between pig behavior to VOCs and illuminance seems to be needed more research.