• Speech Sciences
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• v.11 no.1
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• pp.73-83
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• 2004

Electromyographic studies of the cricopharyngeus muscle using hooked wire electrodes were performed in thyroidectomized patients. The shape of the cricoid cartilage and soft tissue thickness in the postcricoid area were evaluated during pitch elevation and pitch lowering using conventional neck lateral films. The cricopharyngeus muscle simultaneously activated in the initial task of speech and continuously activated. Its activity lessened in the interrogative stress contrast of sentence terminals and increased in the pitch lowered contrast of sentence terminal. On the radiologic findings the cricoid cartilage was tilted backward during high pitched phonation and tilted forward during low pitched phonation. The soft tissue thickness of postcricoid area was thicker at the low pitch than at high pitch. At low pitch the cricoid cartilage paralleled along the vertebral column. This result suggests that the bulging of cricopharyngeus muscle in contraction induce a thickened the postcricoid area thickened, and exert pressure anteriorly exerted on the cricoid cartilage. This contraction of the cricopharyngeus muscle may result in shortening the vocal fold and lowering pitch.

• Biomaterials and Biomechanics in Bioengineering
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• v.1 no.3
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• pp.151-162
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• 2014

Genetically-modified mesenchymal stem cells (GM-MSCs) have emerged as promising therapeutic tools for orthopedic degenerative diseases. GM-MSCs have been widely reported that they are able to increase bone and cartilage tissue regeneration not only by secreting transgene products such as growth factors in a long-term manner, also by inducing MSCs into tissue-specific cells. For example, MSCs modified with BMP-2 gene increased secretion of BMP-2 protein resulting in enhancement of bone regeneration, while MSCs with TGF-b gene did cartilage regeneration. In this review, we introduce several growth factors for gene delivery to MSCs and strategies for bone and cartilage tissue regeneration using GM-MSCs. Furthermore, we describe strategies for strengthening GM-MSCs to more intensively induce tissue regeneration by co-delivery system of multiple genes.

• Journal of Biomedical Engineering Research
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• v.26 no.1
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• pp.37-42
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• 2005

We construct and test the polarization-sensitive optical coherence tomography (PS-OCT) system for imaging porcine and human articular cartilages. PS-OCT is a new imaging technology that provides information regarding not only the tissue structures but tissue components that show birefringence such as collagen. In this study, we measure the cartilage thickness of the porcine joint and the phase retardation due to collagen birefringence. Also, we demonstrate that changes of the collagen fiber orientation could be detected by the PS-OCT system. Finally, differences between normal and damaged human articular cartilage are observed using the PS-OCT system, which is then compared with the regular histology pictures. As a result, the PS-OCT system is proven to be effective for diagnosis of the pathology related to the cartilage. In the future, this technology may be used for discrimination of the collagen types. When combined with endoscope technologies, the PS-OCT images may become a useful tool for in vivo tissue testing.

• Archives of Plastic Surgery
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• v.41 no.1
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• pp.19-28
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• 2014

Recently, in Korea, the septal extension graft from the septum or rib has become a common method of correcting a small or short nose. The success rate of this method has led to the blind faith that it provides superior tip projection and definition, and to the failure to notice its weaknesses. Even if there is a sufficient amount of cartilage, improper separation or fixation might waste the cartilage, resulting in an inefficient operation. Appropriate resection and effective fixation are essential factors for economical rhinoplasty. The septal extension graft is a remarkable procedure since it can control the nasal tip bidirectionally and three dimensionally. Nevertheless, it has a serious drawback since resection is responsible for septal weakness. Safe resection and firm reconstruction of the framework should be carried out. Operating on the basis of the principle of "safe harvest" and rebuilding the structures is important. Further, it is important to learn several techniques to manage septal weakness, insufficient cartilage quantity, and failure of the rigid frame during the surgery.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.17 no.2
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• pp.153-158
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• 1995

A wide variety of deformities can occur following repair of the cleft lip. Especially, cleft lip nasal deformities offer the severe psychologic, esthetic, and functional impairment. We must restore the deformities of alar cartilge, nasal tip, septum, columella, or pyriform aperture. The authors reconstructed the cleft lip nasal deformities using with the alar cartilage rearrangement, postauricular cartilage graft, and/or columellar lengthening. The 3 case reports are presented.

• Journal of Veterinary Clinics
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• v.17 no.1
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• pp.129-133
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• 2000

Based on pathological findings, 5 German shepherds, revealed a mean age of 7.6 years, showing no clinical signs were diagnosed as tracheopathia osteochondroplastica(TOC). Grossly, multiple small-sizecd nodules, appeared as cobble-stones, on the cartilage rings situated in the anterior trachea and the mucosal surface of the epiglottis were showed. Numerous tiny nodules were scattered on the pleural surface of the lung. The vascular walls of the heart were irregular and coarse apearance with calcification. Histopathologically, nodules in the trachea represented an irregular expansion of the underlying tracheal ring with protrusion into the submucosa and consisted of proliferated and calcified cartilage and bone with marrow spaces containing numbers of hematopoietic cells. Pulmonary calcinosis and calcification of the vascular walls of the heart were observed. Our observations suggested that TOC arises form eccondroses and exostoses of the tracheal cartilage rings.

• The Journal of the Korean dental association
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• v.12 no.4
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• pp.269-273
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• 1974

This present investigation was concerned with the content of ash, Mg, Ca, K and N'a of bone, cartilage, incisor teeth and molar teeth in rats of different weights. The results obtained were summarized as follows. 1. Cartilage is the most adequate model for the study in vivo of mechanisms concerned with normal calcification. 2. The percentage of inorganic material in cartilage rose from approx 13% of the dry weight in 5g rat to 46% in the tissue from rats weighing 134g 3. There was no marked change in the content of ash, Ca, Mg, K and Na after eruptions due to the increasing weight.

• Journal of Veterinary Clinics
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• v.28 no.5
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• pp.486-496
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• 2011

A special mesenchymal tissue layer called perichondrium has a chondrogenic capacity and is a candidate tissue for engineering of cartilage. To overcome limited potential for chondrocyte proliferation and re-absorption, we studied a method of cartilage tissue engineering comprising chondrocyte-hydrogel pluronic complex (CPC) and cultured perichondrial cell sheet (cPCs) which entirely cover CPC. For effective cartilage regeneration, cell-sheet engineering technique of high-density culture was used for fabrication of cPCs. Hydrogel pluronic as a biomimetic cell carrier used for stable and maintains the chondrocytes. The human cPCs was cultured as a single layer and entirely covered CPC. The tissue engineered constructs were implanted into the dorsal subcutaneous tissue pocket on nude mice (n = 6). CPC without cPCs were used as a controls (N = 6). Engineered cartilage specimens were harvested at 12 weeks after implantation and evaluated with gross morphology and histological examination. Biological analysis was also performed for glycosaminoglycan (GAG) and type II collagen. Indeed, we performed additional in vivo studies of cartilage regeneration using canine large fullthickness chondrial defect model. The dogs were allocated to the experimental groups as treated chondrocyte sheets with perichondrial cell sheet group (n = 4), and chondrocyte sheets only group (n = 4). The histological and biochemical studies performed 12 weeks later as same manners as nude mouse but additional immunofluorescence study. Grossly, the size of cartilage specimen of cPCs covered group was larger than that of the control. On histological examination, the specimen of cPCs covered group showed typical characteristics of cartilage tissue. The contents of GAG and type II collagen were higher in cPCs covered group than that of the control. These studies demonstrated the potential of such CPC/cPCs constructs to support chondrogenesis in vivo. In conclusion, the method of cartilage tissue engineering using cPCs supposed to be an effective method with higher cartilage tissue gain. We suggest a new method of cartilage tissue engineering using cultured perichondrial cell sheet as a promising strategy for cartilage tissue reconstruction.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.30 no.1
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• pp.17-24
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• 2004

The purpose of this study was to evaluate the stability and efficacy of biologic membrane made of freeze-dried cartilage as a barrier to facilitate guided bone regeneration in experimental non-healing bone defects in the rat mandible. Nine adult Sprague-Dawley rats (400-500g) were used in experiment. 5.0mm in diameter were created on the mandibular angle area by means of slow-speed trephine drill. In microscopic examination, dynamic immature bone forming at 2 weeks and its calcification at 4 weeks were observed. The membrane made of lyophilized cartilage taken from human costal cartilage seems to be very effective for guided bone regeneration as a biologic membrane and the scaffold for attachment of cells or local drug delivery system of growth factor, which may meet the ideal requirement of a barrier membrane and graft materials.

• Proceedings of the Korean Society of Applied Pharmacology
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• 2001.11a
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• pp.14-20
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• 2001

Rheumatoid arthritis (RA) is one of the most typical rheumatic diseases, and is characterized by chronic inflammation, cartilage destruction and joint deformity ［1,2］. During this process, profound hypertrophic changes of the synovium with infiltration of immune cells, increased vascularity, and hyperplasia result in the formation of a synovial pannus that invades cartilage and bone ［3］. In early stages of RA, the synovial membrane begins to invade the cartilage. In established RA, the synovial membrane becomes transformed into inflammatory tissue, the pannus (Fig. 1). The cell types that occupy cartilage-pannus junctions include synovial macrophages, fibroblasts, mast cells, polymorphonuclear lymphocytes (PMNs), and displaced, probably differentiated condrocytes ［4-6］. Recent studies of rheumatoid synovial tissue have demonstrated localized accumulations of mast cells and evidence of their activation/degranulation［7］.