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Development of SCAR Marker for Identification of the Perilla Species

  • Lee, Myoung-Hee;Yang, Ki-Woong;Ha, Tae Joung;Jung, Chan-Sik;Pae, Suk-Bok;Hwang, Chung-Dong;Park, Chang-Hwan;Baek, In-Youl;Kim, Hyeon-Kyeong;Park, Soon-Ki
    • Korean Journal of Breeding Science
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    • v.43 no.4
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    • pp.265-272
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    • 2011
  • This study is to generate SCARs markers for identification of Perilla species. A SCAR is a genomic DNA fragment at a single genetically defined locus that is identified by PCR amplification using a pair of specific oligonucleotide primers. We derived SCARs by sequencing and cloning the both ends of the amplified products of RAPD markers. Sixteen sequence-specific primers were synthesized from eight RAPD markers, which were completely sequenced. We developed the species-specific SCAR markers which could be used successfully in detecting genetic variation in four Perilla species. These markers could be used to verify species-origins of various forms of Perilla germplasms.

A Study Shrinkage Analysis of Injection mold using Regression Analysis (회귀분석법을 이용한 사출금형의 수축률 분석에 관한 연구)

  • RYU, M.R.;BAE, H.E.;PARK, J.H.;PARK, J.S.;PARK, S.H.;LEE, D.H.;LEE, S.B.
    • Journal of the Korean Society of Mechanical Technology
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    • v.13 no.3
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    • pp.113-118
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    • 2011
  • It is not easy to predict the shrinkage rate of a plastic injection mold in its design process. The shrinkage rate should be considered as one of the important performances to produce the reliable products. The shrinkage rate can be determined by using the CAE tools in the design produces. However, since the analysis can take minutes to hours, the high computational costs of performing the analysis limit their use in design optimization. Therefore this study was carried out to presume for mutual relation of analysis condition to get the optimum average shrinkage by regression analysis. The results shown that coefficient of determination of regression equation has a fine reliability over 87% and regression equation of average shrinkage is made by regression analysis.

Development and Performance Test of Hydraulic PTO for 50㎾ Class Rotating Body Type Wave Energy Converter (50㎾급 동체회전형 파력발전시스템 유압식 전력변환장치 개발 및 성능시험)

  • Choi, Kyung-Shik;Yang, Dong-Soon;Park, Shin-Yeol;Cho, Byung-Hak
    • Journal of the Korean Society of Mechanical Technology
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    • v.13 no.3
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    • pp.99-106
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    • 2011
  • 50kW급 동체회전형 파력발전시스템(WEC;wave energy converter)의 전력변환장치(PTO; power take-off)를 설계 제작한 후 성능시험을 하였다. 파력발전시스템은 2개의 실린더형 동체가 회전관절로 연결된 구조로 수면의 반정도 잠기는 구조로 되어있다. 파랑에 의해 유도된 회전관전의 움직임이 유압실린더에 힘을 가해주며, 유압실린더는 고압의 작동유를 축압기를 경유하여 발전기에 체결된 유압모터로 공급한다. 유압식 PTO은 유압실린더가 왕복운동하는 움직임을 이용하여 고품질의 전력을 생산하는데 효과전인 수단을 제공한다. 파력발전시스템의 경제성은 PTO의 에너지 변환 효율에 크게 의존한다. 발전기를 AC 380V 전력계통에 연계시킨 후, 발전기에서 나오는 출력이 5, 20, 35, 43kW 일때 PTO 전체와 개별기기에 대한 효율을 측정하였다. 본 논문에서 유압식 PTO시스템의 설계에 대해 설명하였으며 효율 향상에 초점을 맞추어 PTO 성능대해 분석하였다.

Effect of Cell Wall-Wounding Reagents on Agrobacterium-mediated Barley Seedling Transformation (Agrobacterium 이용 보리묘 형질전환에 대한 세포벽 상해물질의 효과)

  • Choi, Jang-Won;Park, Hee-Sung
    • Journal of agriculture & life science
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    • v.44 no.1
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    • pp.9-15
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    • 2010
  • Barley, a monocotyledonous plant, is relatively recalcitrant to the process of Agrobacterium-mediated genetic transformation. In this study, seedlings of six barley cultivars (Keunal-1-Ho, Saessal, Ol, Saechalssal, Seodunchal and Pungsanchalssal) were injured using alkali, oxidizing or reducing agents. They were then transformed using Agrobacterium via vacuum infiltration for the analysis of comparative GUS gene expression. It was determined that chemical injuries causing a slight growth retardation could overall enhance the GUS transformation rate. Hydrogen peroxide was determined to be the most effective.

Study on the Development of Multi Heat Supply Control Algorithm in Apartment Building of District Heating Energy (지역난방 에너지 공동주택의 다중 열공급 제어 알고리즘 개발에 관한 해석적 연구)

  • Byun, J.K.;Choi, Y.D.;Park, M.H.;Shin, J.K.
    • Journal of the Korean Society of Mechanical Technology
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    • v.13 no.2
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    • pp.63-70
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    • 2011
  • In the present study, we developed optimal heat supply algorithm which minimizes the heat loss through the distribution pipe line in group energy apartment. Heating load variation of group energy apartment building in accordance with outdoor air temperature was predicted by the correlation obtained from calorimeter measurements of whole households of apartment building. Supply water temperature and mass flow rate were conjugately controlled to minimize the heat loss rate through distribution pipe line. Group heating apartment located in Hwaseong city, Korea, which has 1,473 households divided in 4 regions, was selected as the object apartment for verifying the present heat supply control algorithm. Compared to the original heat supply system, 10.4% heat loss rate reduction can be accomplished by employing the present control algorithm.

A Technique for Improving the Quality of Stereo DEM Using Texture Filters

  • Kim, Kwang-Eun
    • Korean Journal of Remote Sensing
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    • v.18 no.3
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    • pp.181-186
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    • 2002
  • One of the most important procedure in stereo DEM generation is the stereo matching process which finds the conjugate pixels in a pair of stereo imagery. In order to be found as conjugate pixels, the pixels should have distinct spatial feature to be distinguished from other pixels. However, in the homogeneous areas such as water covered or forest canopied areas, it is very difficult to find the conjugate pixels due to the lack of distinct spatial feature. Most of erroneous elevation values in the stereo DEM are produced in those homogeneous areas. This paper presents a simple method for improving the quality of stereo DEM utilizing the texture filters. An entropy filter was applied to one of the input stereo imagery to extract very homogeneous areas before stereo matching process. Those extracted homogeneous areas were excluded from being candidates for stereo matching process. Also a statistical texture filter was applied to the generated elevation values before the interpolation process was applied in odor to remove the remaining anomalous elevation values. Stereo pair of SPOT level 1B panchromatic imagery were used for the experiments. The results showed that by utilizing the texture filters as a pre and a post processor of stereo matching process, the quality of the stereo DEM could be dramatically improved.

A Comparison of ERBE and AVHRR Longwave Flux Estimates (ERBE와 AVHRR에 의하여 추정된 지구의 장파복사량 비교)

  • 오성남
    • Korean Journal of Remote Sensing
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    • v.6 no.2
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    • pp.75-88
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    • 1990
  • NOAA 위성의 narrow-band AVHRR(Advanced Very. High Resolution Radiometer) 적 외선 채널과 broad-band 0.2 - 50$\mu\textrm{m}$ 영역의 ERBE(Earth Radiation Budget Experiment) scanning instrument에 의하여 관측된 radiance로부터 추정된 지구의 대기 외장파복사량 (Outgoing Longwave Radiation:OLR) 이 비교조사되었다. 이를 위하여 1985년 4월, 7월, 10월과 1986년 1월에 위성에서 관측된 radiance를 각각 이용하였고 비교된 OLR은 위도와 경도가 각각 2.5$^{\circ}$ 간격으로 구분된 grid내에서 일치(collocate)시켜 지역별(zonal), 그리고 전지구(global)규모 로 비교되었다. ERBE와 AVHRR에 의하여 각각 추정된 OLR값의 차(ERBE minus AVHRR)에 의한 분석 결과는 주간의 경우 -1~2 W/m$^2$의 값과 야간의 경우 4~7 W/m$^2$의 값으로 비교적 좋은 일치를 보였지만 이들의 RMS는 하절(6월)에 12 W/m$^2$와 동절(12월)에 5 W/m$^2$의 값으로 다소 높은 차이를 보였다. 한편, 이들 OLR값을 관측지역에 따라 큰 차 이를 나타내어 사막지역과 아열대고기압(subtropical ocean)대에서는 상반된 결과를 보였다. 이들 지역에 대한 차이는 지역적 기온구조와 지표온도의 영향을 다 고려하지 못하고 OLR 측정치를 도 출하는 대기복사모델(radiation model)의 regional systematic bias에서 기인된 것으로 해석된다. 즉 사막의 지표역전층에 대한 AVHRR과 해면의 대기구조에 대한 ERBE의 OLR은 상반된 영향을 보였다.

An Adjustment for a Regional Incongruity in Global land Cover Map: case of Korea

  • Park Youn-Young;Han Kyung-Soo;Yeom Jong-Min;Suh Yong-Cheol
    • Korean Journal of Remote Sensing
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    • v.22 no.3
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    • pp.199-209
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    • 2006
  • The Global Land Cover 2000 (GLC 200) project, as a most recent issue, is to provide for the year 2000 a harmonized land cover database over the whole globe. The classifications were performed according to continental or regional scales by corresponding organization using the data of VEGETATION sensor onboard the SPOT4 Satellite. Even if the global land cover classification for Asia provided by Chiba University showed a good accuracy in whole Asian area, some problems were detected in Korean region. Therefore, the construction of new land cover database over Korea is strongly required using more recent data set. The present study focuses on the development of a new upgraded land cover map at 1 km resolution over Korea considering the widely used K-means clustering, which is one of unsupervised classification technique using distance function for land surface pattern classification, and the principal components transformation. It is based on data sets from the Earth observing system SPOT4/VEGETATION. Newly classified land cover was compared with GLC 2000 for Korean peninsula to access how well classification performed using confusion matrix.

A Hill-Sliding Strategy for Initialization of Gaussian Clusters in the Multidimensional Space

  • Park, J.Kyoungyoon;Chen, Yung-H.;Simons, Daryl-B.;Miller, Lee-D.
    • Korean Journal of Remote Sensing
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    • v.1 no.1
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    • pp.5-27
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    • 1985
  • A hill-sliding technique was devised to extract Gaussian clusters from the multivariate probability density estimates of sample data for the first step of iterative unsupervised classification. The underlying assumption in this approach was that each cluster possessed a unimodal normal distribution. The key idea was that a clustering function proposed could distinguish elements of a cluster under formation from the rest in the feature space. Initial clusters were extracted one by one according to the hill-sliding tactics. A dimensionless cluster compactness parameter was proposed as a universal measure of cluster goodness and used satisfactorily in test runs with Landsat multispectral scanner (MSS) data. The normalized divergence, defined by the cluster divergence divided by the entropy of the entire sample data, was utilized as a general separability measure between clusters. An overall clustering objective function was set forth in terms of cluster covariance matrices, from which the cluster compactness measure could be deduced. Minimal improvement of initial data partitioning was evaluated by this objective function in eliminating scattered sparse data points. The hill-sliding clustering technique developed herein has the potential applicability to decomposition of any multivariate mixture distribution into a number of unimodal distributions when an appropriate diatribution function to the data set is employed.

Structural Determination of Fatty Acyl Groups of Phospholipids by Fast Atom Bombardment Tandem Mass Spectrometry of Sodium Adduct Molecular Ions

  • 김영환;유종신;김명수
    • Bulletin of the Korean Chemical Society
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    • v.18 no.8
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    • pp.874-880
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    • 1997
  • Various classes of phospholipids were investigated for the structural determination of fatty acyl groups by fast atom bombardment tandem mass spectrometry (FAB-MS/MS). Phospholipids were desorbed by FAB as molecules chelated with sodium ion (or ions). Collision-induced dissociation (CID) of intact sodium adduct molecular ions ([M+Na]+, [M-H+2Na]+ or [M+Na-2H]-) produced a series of homologous fragment ions via the charge-remote fragmentation along the fatty acid chains. These ions were found useful to locate the double bond positions even for the polyunsaturated fatty acid chains. The regiospecificity of the acyl chain linkages in phosphatidylcholine (PC) could also be determined based on the ratio of relative abundance of the product ions (i.e., [M+Na-85-R2COOH]+ vs [M+Na-85-R1COOH]+) in CID-MS/MS of [M+Na]+. These are generated by the loss of fatty acyl groups at sn-1 and sn-2, respectively, together with the choline group. In all the phospholipid compounds investigated, loss of the fatty acid at the sn-2 position was dominant. The present method was applied to the structural determination of molecular species of phosphatidylglycerols (PG) isolated from cyanobacterium Synechocystis sp. PCC 6803.